Easy access to aroma active unsaturated γ-lactones by addition of modified titanium homoenolate to aldehydes     

Frerot E  Bagnoud A 《Journal of agricultural and food chemistry》2011,59(8):4057-4061

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Exploration of plant genomes in the FLAGdb++ environment     

Sandra Dèrozier  Franck Samson  Jean-Philippe Tamby  Cécile Guichard  Véronique Brunaud  Philippe Grevet  Séverine Gagnot  Philippe Label  Jean-Charles Leplé  Alain Lecharny  Sébastien Aubourg 《Plant methods》2011,7(1):1-10

Background

Efficient high throughput screening systems of useful mutants are prerequisite for study of plant functional genomics and lots of application fields. Advance in such screening tools, thanks to the development of analytic instruments. Direct analysis in real-time (DART)-mass spectrometry (MS) by ionization of complex materials at atmospheric pressure is a rapid, simple, high-resolution analytical technique. Here we describe a rapid, simple method for the genetic discrimination of intact Arabidopsis thaliana mutant seeds using metabolic profiling by DART-MS.

Results

To determine whether this DART-MS combined by multivariate analysis can perform genetic discrimination based on global metabolic profiling, intact Arabidopsis thaliana mutant seeds were subjected to DART-MS without any sample preparation. Partial least squares-discriminant analysis (PLS-DA) of DART-MS spectral data from intact seeds classified 14 different lines of seeds into two distinct groups: Columbia (Col-0) and Landsberg erecta (Ler) ecotype backgrounds. A hierarchical dendrogram based on partial least squares-discriminant analysis (PLS-DA) subdivided the Col-0 ecotype into two groups: mutant lines harboring defects in the phenylpropanoid biosynthetic pathway and mutants without these defects. These results indicated that metabolic profiling with DART-MS could discriminate intact Arabidopsis seeds at least ecotype level and metabolic pathway level within same ecotype.

Conclusion

The described DART-MS combined by multivariate analysis allows for rapid screening and metabolic characterization of lots of Arabidopsis mutant seeds without complex metabolic preparation steps. Moreover, potential novel metabolic markers can be detected and used to clarify the genetic relationship between Arabidopsis cultivars. Furthermore this technique can be applied to predict the novel gene function of metabolic mutants regardless of morphological phenotypes.  相似文献   

The reproductive potential of the root-knot nematode <Emphasis Type="Italic">Meloidogyne incognita</Emphasis> is affected by selection for virulence against major resistance genes from tomato and pepper     

Caroline Djian-Caporalino  Sergio Molinari  Alain Palloix  Aurelio Ciancio  Ariane Fazari  Nathalie Marteu  Nicolas Ris  Philippe Castagnone-Sereno 《European journal of plant pathology / European Foundation for Plant Pathology》2011,131(3):431-440

The emergence of virulent root-knot nematode populations, able to overcome the resistance conferred by some of the resistance genes (R-genes) in Solanaceous crops, i.e., Mi(s) in tomato, Me(s) in pepper, may constitute a severe limitation to their use in the field. Research has been conducted to evaluate the durability of these R-genes, by comparing the reproduction of several laboratory-selected and wild virulent Meloidogyne incognita isolates, on both susceptible and resistant tomatoes and peppers. We first show that the Me1 R-gene in pepper behaves as a robust R-gene controlling avirulent and virulent Me3, Me7 or Mi-1 isolates. Although the reproductive potential of the virulent isolates was highly variable on susceptible and resistant plants, we also confirm that virulence is highly specific to a determined R-gene on which selection has occurred. Another significant experimental result is the observation that a reproductive fitness cost is associated with nematode virulence against Mi-1 in tomato and Me3 and Me7 in pepper. The adaptative significance of trade-offs between selected characters and fitness-related traits, suggests that, although the resistance can be broken, it may be preserved in some conditions if the virulent nematodes are counter-selected in susceptible plants. All these results have important consequences for the management of plant resistance in the field.  相似文献   

The bryophyte layer in a calcareous grassland after a decade of contrasting mowing regimes     

Alain Vanderpoorten  Louis-Marie Delescaille 《Biological conservation》2004,117(1):11-18

Analysis of variance and redundancy analysis were used to study the effects of mowing regime and herb canopy structure on the bryophyte layer of a calcareous grassland in southern Belgium. Species richness in mown and control plots did not significantly differ. In control plots, a number of grassland species were able to survive within a grassy-shrubby fringe 15 years after shrub cutting. This suggests that there might be a time-lag of at least 20 years before cessation of mowing results in a significant decrease in species number per m². Mown plots were mostly characterized by the almost complete cover of large pleurocarps. Mowing resulted in almost continuous herb and moss layers that were unfavourable for a number of annual bryophytes, many of which are of high conservation interest. It is suggested that grazing, which increases the number of species, especially the number of pioneers, is likely to be more beneficial to bryophytes.  相似文献   

Heterocyclic acetals from glycerol and acetaldehyde in Port wines: evolution with aging     

da Silva Ferreira AC  Barbe JC  Bertrand A 《Journal of agricultural and food chemistry》2002,50(9):2560-2564

In Port wine, isomers of glycerol and acetaldehyde acetals have been found at total contents ranging from 9.4 to 175.3 mg/L. During oxidative aging, the concentrations of the 5-hydroxy-2-methyl-1,3-dioxane and 4-hydroxymethyl-2-methyl-1,3-dioxolane isomers increased with time showing a linear correlation (r > 0.95). The flavor threshold for the mixture of the four isomers was evaluated in wine at 100 mg/L. Thus, it is expected that they contribute to "old Port wine" aroma in wines older than 30 years. Experiments with model solutions and wine clearly demonstrated that SO(2) combines with acetaldehyde and blocks the acetalization reaction.  相似文献   

Effects of hydrophilic plasticizers on mechanical, thermal, and surface properties of chitosan films   总被引：6，自引：0，他引：6  

Suyatma NE  Tighzert L  Copinet A  Coma V 《Journal of agricultural and food chemistry》2005,53(10):3950-3957

Chitosan films were plasticized with four hydrophilic compounds, namely, glycerol (GLY), ethylene glycol (EG), poly(ethylene glycol) (PEG), and propylene glycol (PG). Our objective was to investigate the effect of plasticizers on mechanical and surface properties of chitosan films. The stability of plasticized films was observed by storage for 3 and 20 weeks in an environmental chamber at 50 +/- 5% RH and 23 +/- 2 degrees C. Plasticization improves the chitosan ductility, and typical stress-strain curves of plasticized films have the features of ductile materials, except the film made with 5% PG that exhibits as a brittle polymer and shows an antiplasticization effect. In most cases, the elongation of plasticized films decreases with the storage time, which might be due to the recrystallization of chitosan and the loss of moisture and plasticizer from the film matrix. Although at the beginning the mechanical properties of films made with PG, at high plasticizer concentration, are comparable to those of films made with EG, GLY, and PEG, their stability is poor and they tend to become brittle materials. The surface properties, analyzed by contact angle measurement, reveal that plasticization increases film hydrophilicity. It is found that GLY and PEG are more suitable as chitosan plasticizers than EG and PG by taking into account their plasticization efficiency and storage stability. Furthermore, a plasticizer concentration of 20% (w/w) with GLY or PEG seemingly is sufficient to obtain flexible chitosan film with a good stability for 5 months of storage.  相似文献   

Variation in the distribution of monosaccharides in soil fractions in the mounds of termites with different feeding habits (Senegal)     

Saïdou Sall  Alain Brauman  Saliou Fall  Corinne Rouland  Edouard Miambi  Jean-Luc Chotte 《Biology and Fertility of Soils》2002,36(3):232-239

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Volatile constituents of Semnostachya menglaensis Tsui     

Naef R  Velluz A  Mayenzet F  Starkenmann C  Sun HD 《Journal of agricultural and food chemistry》2005,53(23):9161-9164

Semnostachya menglaensis Tsui (Acanthaceae) is a rare plant indigenous to Mengla in the tropical rainforest of the Xishuangbanna prefecture in the south of Yunnan province, People's Republic of China. When the leaves are crushed, a characteristic smell of basmati rice or pandan leaves develops. Their hexane extract, prepared from a specimen growing in a greenhouse of the botanical garden of the Kunming Institute of Botany, contains 1-(3,4,5,6-tetrahydro-2-pyridyl)-1-propanone (41.2%) and 1-(1,4,5,6-tetrahydro-2-pyridyl)-1-propanone (37.5%) which constitute the main part of the volatile compounds. Minor components are 1-(3,4,5,6-tetrahydro-2-pyridyl)-1-ethanone (4.9%), 1-(1,4,5,6-tetrahydro-2-pyridyl)-1-ethanone (4.8%), 1-(2-piperidyl)-1-propanone (5.2%), 1-octen-3-ol (3.2%), 1-octen-3-one (1.9%), and 3-octanol and 1-(2-pyridyl)-1-propanone in trace amounts.  相似文献   

A simple, rapid syncytial-inhibition test for antibodies to bovine leukemia virus.   总被引：1，自引：1，他引：0       下载免费PDF全文

A S Greig  S Chander  B Samagh    A M Bouillant 《Canadian journal of veterinary research》1978,42(4):446-451

Cocultivation of equal numbers of cells from a fetal lamb kidney line infected with bovine leukemia virus and African green monkey (Vero) cells results in the rapid production of syncytia. The effect was blocked or inhibited by serum containing antibodies to bovine leukemia virus. A serological test based on syncytial inhibition was compared to the agar gel immunodiffusion test and the modified direct complement fixation test for the detection of bovine leukemia virus antibodies in sera from leukosis-free cattle, cases of adult enzootic bovine lymphosarcoma and cattle from herds in contact with enzootic lymphosarcoma. The results showed the syncytial inhibition test to react positively with sera from all cases of adult enzootic lymphosarcoma, but to be much less sensitive than the other tests in detecting bovine leukemia virus antibodies in sera of exposed animals.  相似文献   

Serological, Molecular, and Pathotype Diversity of Pepper veinal mottle virus and Chili veinal mottle virus   总被引：1，自引：0，他引：1  

Moury B  Palloix A  Caranta C  Gognalons P  Souche S  Selassie KG  Marchoux G 《Phytopathology》2005,95(3):227-232

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