Tanglehead in Southern Texas: A Native Grass with an Invasive Behavior     

David B. Wester  Fred C. Bryant  Aaron D. Tjelmeland  Joshua L. Grace  Scott L. Mitchell  John T. Edwards  Fidel Hernández  Robert K. Lyons  Megan K. Clayton  Sandra Rideout-Hanzak  Richard V. Machen  J. Alfonso Ortega-S. 《Herpetological Monographs》2018,40(2):37-44

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Identification and quantification of xanthophyll esters, carotenes, and tocopherols in the fruit of seven Mexican mango cultivars by liquid chromatography-atmospheric pressure chemical ionization-time-of-flight mass spectrometry [LC-(APcI(+))-MS     

Ornelas-Paz Jde J  Yahia EM  Gardea-Bejar A 《Journal of agricultural and food chemistry》2007,55(16):6628-6635

A liquid chromatography-mass spectrometry (LC-MS) method was developed to simultaneously identify and quantify carotenoids and tocopherols in the fruit of seven mango cultivars grown in Mexico. Fruit maturity was characterized objectively, and carotenoids and tocopherols were isolated by solvent extraction and analyzed by HPLC coupled to a C30 stationary phase and diode array, fluorescence, and mass (time-of-flight) detectors. All cultivars had a similar carotenoid pattern, in which all-trans-beta-carotene and dibutyrates of all-trans-violaxanthin and 9-cis-violaxanthin were the most abundant. The content of all-trans-beta-carotene ranged between 0.4 and 2.8 mg/100 g, and 'Haden' and 'Ataulfo' mangoes had the highest amount. The amounts of all-trans-violaxanthin and 9-cis-violaxanthin (as dibutyrates) ranged between 0.5 and 2.8 mg/100g and between 0.4 and 2.0 mg/100 g, respectively. The content of alpha-tocopherol was low (200-500 microg/100 g). The results of this study indicate that all-trans-beta-carotene, all-trans-violaxanthin, and 9-cis-violaxanthin are the most abundant carotenoids in mango grown in Mexico.  相似文献   

The identification of a VDAC-like protein involved in the interaction of Babesia bigemina sexual stages with Rhipicephalus microplus midgut cells     

Rodríguez-Hernández E  Mosqueda J  Alvarez-Sánchez ME  Neri AF  Mendoza-Hernández G  Camacho-Nuez M 《Veterinary parasitology》2012,186(3-4):538-541

Oestrosis is highly prevalent in Mediterranean countries. Understanding the life cycle of Oestrus ovis is crucial to design effective control measures of this myiasis, largely based on the use of macrocyclic lactones. We carried out a survey of ovine oestrosis in the island of Majorca (Spain) and found that 46.03% of animals were infested in a 13-month period. Interestingly, we found significant differences in oestrosis prevalences in winter and autumn when separating the animals by group of age (P<0.001). Pearson correlation analysis showed that prevalence in lambs younger than 4 months was significantly affected by changes in air temperature (P<0.05), but this association was not significant in adult sheep (P=0.081). Chronic infestations or unsystematic treatments may explain confusing results in adult sheep. Observing the evolution of the disease in young lambs, we determined that the hypobiotic period took place from October to February and the beginning of fly activity occurred between May and June. Interannual variations in oestrosis prevalence indicate the need of monitoring the disease to establish the appropriate timing of treatments. We hypothesize that lambs are better indicators of the seasonality of oestrosis than their older counterparts. Furthermore, we propose that observing O. ovis infestations in young lambs can be used as an efficient early warning system of fly activity, to be applied in future control programs.  相似文献   

Serological proteome analysis of Staphylococcus aureus isolated from sub-clinical mastitis     

Tedeschi G  Taverna F  Negri A  Piccinini R  Nonnis S  Ronchi S  Zecconi A 《Veterinary microbiology》2009,134(3-4):388-391

Staphylococcus aureus is the most common aetiologic agent of contagious bovine mastitis. Studies of the molecular epidemiology of S. aureus strongly suggest that some genetic subsets of strains are particularly well adapted for causing infections in cattle. This communication reports the setup of experimental protocols to identify the immunogenic proteins expressed by one of the most common field isolated strain of S. aureus responsible for sub-clinical mastitis cases. The serological proteome analysis (SERPA) approach applied consists of three main steps: two-dimensional electrophoresis-based separation of the proteins contained in field isolated S. aureus extracts enriched for surface proteins, detection of immunogenic spots using anti-serum collected from sub-clinical mastitis cases and identification of antigens by mass spectrometric-based methodologies. The study allowed to identify three immunogenic proteins: DNAase translocase FtsK, ribosomal proteins S1 and a Tell-like protein.  相似文献   

Electrochemotherapy for the treatment of squamous cell carcinoma in cats: a preliminary report     

Spugnini EP  Vincenzi B  Citro G  Tonini G  Dotsinsky I  Mudrov N  Baldi A 《Veterinary journal (London, England : 1997)》2009,179(1):117-120

Squamous cell carcinomas (SCC) of the skin are commonly described in cats. Reported treatments include surgery, radiation therapy and photodynamic therapy. This preliminary study reports on the management of these lesions combining the local administration of bleomycin (plus hyaluronidase for a more uniform distribution) with permeabilizing biphasic electric pulses. Nine cats with SCC graded T(2)-T(4) were treated over a 5 year period, and each cat received two sessions of electrochemotherapy (ECT) 1 week apart. The side effects of this treatment were minimal and limited to mild erythema of the nose. Seven of the cats (77.7%) had a complete response lasting up to 3 years. ECT seems to be a safe and effective option for the treatment of feline sun-induced squamous cell carcinomas and warrants further investigation.  相似文献   

Distribution of Crenosoma vulpis and Eucoleus aerophilus in the lung of free-ranging red foxes (Vulpes vulpes).     

Alicia Nevárez  Alfonso López  Gary Conboy  William Ireland  David Sims 《Journal of veterinary diagnostic investigation》2005,17(5):486-489

Crenosoma vulpis and Eucoleus aerophilus are nematode parasites that can cause verminous pneumonia in wild carnivores. There is a paucity of information regarding the distribution of parasites in the lungs and the relationship between histopathological and parasitological diagnoses in naturally infected foxes. The objectives of this study were: first, to study the lobar and airway distribution of C. vulpis and E. aerophilus in wild red foxes and second, to investigate the relationship between fecal and histopathological diagnoses. Samples from 6 sites of the lung and fecal contents were obtained from 51 wild foxes in Prince Edward Island. By fecal examination, 78.4% of wild foxes tested positive for C. vulpis and 68.6% for E. aerophilus. In contrast, 66.6% and 49% of foxes had histopathological evidence of C. vulpis and E. aerophilus in the lungs, respectively. Anatomically, C. vulpis was observed in the small bronchi and bronchioles of all pulmonary lobes whereas E. aerophilus was restricted to the large bronchi and the caudal lobes. Affected airways exhibited severe epithelial glandular hyperplasia and bronchiolar mucous metaplasia. It was concluded that C. vulpis is widely distributed in airways of all pulmonary lobes, whereas E. aerophilus is mainly restricted to the bronchi of caudal lobes. Also, this study showed that histological examination of lung underestimates the infection with E. aerophilus.  相似文献   

Priapism secondary to penile metastasis in a dog          下载免费PDF全文

Rogers L  López A  Gillis A 《The Canadian veterinary journal. La revue veterinaire canadienne》2002,43(7):547-549

A 4-year-old, male Newfoundland cross was presented for lethargy, anorexia, and dysuria. The main clinical finding was an enlarged and painful prostate gland. While the dog was hospitalized, priapism developed. Following euthanasia, microscopic examination revealed that a carcinoma involving both bladder and prostate gland had widely metastasized to the penile vasculature.  相似文献   

Quantitative modification of the testicular structure in pigs fed with anabolic doses of clenbuterol     

Blanco A  Artacho-Pérula E  Flores-Acuña R  Agüera E  Monterde JG 《Veterinary research》2002,33(1):47-53

Morphological and structural data of the testes of thirty male pigs were recorded in order to evaluate the effects of an anabolic clenbuterol treatment. Pigs aged 6 months were randomly allocated to one of three experimental groups. In two treated groups, the animals were fed with anabolic doses of clenbuterol (1 ppm); in the CLB group (n = 10) clenbuterol was given until they were slaughtered (treatment period = 3 months) whereas in the CLBW group (n = 10) the clenbuterol was withdrawn two weeks before slaughter (treatment period = 2.5 months); clenbuterol was not given to the pigs of the control group (n = 10). Stereological estimations of the tissular volume fraction and tubular volume density were applied to quantify the structural constituents of the testes. The results showed an increased volume fraction of the testicular interstitium especially in the Leydig cell population, as a result of the clenbuterol treatment. The increase in the nuclear volume fraction of the Leydig cells was the more persistent change in the variations recorded in both treated groups with respect to the control. A regression of the seminal epithelium was also recorded in the treated animals. The rest of the structural parameters were closer to the normal figures in the CLBW group, suggesting a recovery of the testicular structure when clenbuterol was withdrawn.  相似文献   

Comparison of Agar Dilution and E-test for antimicrobial susceptibility testing of Campylobacter coli isolates recovered from 80 Ontario swine farms          下载免费PDF全文

Norma P. Varela  Robert Friendship  Cate Dewey    Alfonso Valdivieso 《Canadian journal of veterinary research》2008,72(2):168-174

The primary aim of this study was to evaluate the level of agreement of the E-test for in vitro antimicrobial susceptibility testing of Campylobacter coli using the agar dilution technique, which is the approved method. A convenience sample of 80 Ontario swine farms was chosen for this study; each farm was visited from January to June 2004. A total of 233 isolates of C. coli were tested for susceptibility to 10 antimicrobials by agar dilution and the E-test. Performance of the tests was evaluated using 7 quality control strains: Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 29213, Campylobacter jejuni ATCC 33560, and Campylobacter coli ATCC 33559 for the E-test and E. coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, and C. jejuni ATCC 33560 for the agar dilution test. Weighted Cohen's kappa and prevalence-adjusted bias-adjusted kappa (PABAK) tests were used for statistical analysis. The E-test and agar dilution test results had a strong agreement when resistance to streptomycin and tetracycline were evaluated (weighted kappa: 0.68 and 0.66, respectively). However, marked disagreement was detected when testing susceptibility to nalidixic acid and ampicillin (0.15 and 0.22, respectively). Almost perfect agreement was detected by PABAK when testing susceptibility to gentamicin (0.99). Agreement was found to be moderate for ciprofloxacin, azithromycin, clindamycin, erythromycin, and chloramphenicol. Although the level of agreement between the E-test and agar dilution depended on the antimicrobial being tested, the E-test always detected a lower proportion of resistant isolates compared to agar dilution.  相似文献   

Sodium Chloride Effects on Water and Nutrient Uptake Efficiency in Sarcocornia fruticosa Containerized Production     

Pedro García-Caparrós  Alfonso Llanderal 《Communications in Soil Science and Plant Analysis》2016,47(20):2306-2314

The ability to produce native plants well adapted to the saline conditions without the production of nutrient-rich runoff will be a boon to nurseries hoping to reduce their environmental contamination impact and water use while at the same time producing quality plants to be used in the restoration of saline lands. Sarcocornia fruticosa plants were grown for 8 weeks in plastic containers with a source of sphagnum peat moss and perlite (80:20 v/v) to evaluate the effect of two salinity levels (2.0 (low-salinity treatment) and 7.5 dS m^?1 (high-salinity treatment)) on plant growth, nutrient concentration in leachate and water and nutrient uptake efficiency and their losses. Leachate was collected to determine the runoff volume and composition, which included nitrate-nitrogen (NO₃^–N), phosphate-phosphorus (PO₄^3–P) and potassium (K⁺) concentrations. Plant dry weight (DW) and nutrient content were determined in plants at the beginning and at the end of the experiment to establish the nutrient balance. Increasing salinity levels of irrigation water did not reduce either the plant DW or the water-use efficiency (WUE), but increased the volume of leachate per plant. The nutrient concentrations in leachates without significant differences between salt treatments exceeded the thresholds established by environmental guidelines, leading to a great risk of pollution. Based on nutrient balance, the irrigation with a higher salinity level reduced the plant nutrient uptake efficiency (10%, 18% and 12% for nitrogen (N), phosphorus (P) and potassium (K), respectively) and increased the nutrient losses (6% N, 7% P and 8% K), resulting in the recommendation to grow this species with the low salinity level based on the highest nutrient-use efficiency and the lowest levels of nutrient losses.  相似文献