Mycoplasma mastitis in dairy cattle herds in Saudi Arabia     

Al-Abdullah HA  Fadl EA 《The Veterinary record》2006,159(3):88-89

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A new iridoid glycoside from Linaria genestifolia     

Ahmad VU  Kousar F  Zubair M  Khan A  Ali MS  Choudhary MI  Sener B 《Fitoterapia》2006,77(1):12-14

A new iridoid glycoside named genestifolioside (1) was isolated from the ethanol extract of Linaria genestifolia. Its structure was defined by spectral analysis.  相似文献   

Caspases 3 and 7: key mediators of mitochondrial events of apoptosis     

Lakhani SA  Masud A  Kuida K  Porter GA  Booth CJ  Mehal WZ  Inayat I  Flavell RA 《Science (New York, N.Y.)》2006,311(5762):847-851

The current model of apoptosis holds that upstream signals lead to activation of downstream effector caspases. We generated mice deficient in the two effectors, caspase 3 and caspase 7, which died immediately after birth with defects in cardiac development. Fibroblasts lacking both enzymes were highly resistant to both mitochondrial and death receptor-mediated apoptosis, displayed preservation of mitochondrial membrane potential, and had defective nuclear translocation of apoptosis-inducing factor (AIF). Furthermore, the early apoptotic events of Bax translocation and cytochrome c release were also delayed. We conclude that caspases 3 and 7 are critical mediators of mitochondrial events of apoptosis.  相似文献   

A Recessive Allele (tgr-1) Conditioning Tomato Resistance to Geminivirus Infection Is Associated with Impaired Viral Movement     

Bian XY  Thomas MR  Rasheed MS  Saeed M  Hanson P  De Barro PJ  Rezaian MA 《Phytopathology》2007,97(8):930-937

ABSTRACT Begomoviruses (the family Geminiviridae) are transmitted by the whitefly Bemisia tabaci and contain monopartite or bipartite circular single-stranded (ss)DNA genomes. They have emerged as severe problems in the production of agricultural and horticultural crops worldwide. Here, we report the identification of a tomato breeding line, FLA653, that confers a high level of resistance to Tomato leaf curl virus (TLCV, monopartite). Genetic analysis indicated that the resistance is controlled by a single recessive allele named tgr-1, which is in contrast to previous reports that multiple genetic factors are involved in tomato resistance to begomoviruses. Particle bombardment of an infectious TLCV DNA construct into the detached leaves of FLA653 resulted in the viral replication, but the viral ssDNA accumulated at a much lower level than that in susceptible controls. In situ localization of TLCV in the bombarded leaves suggests that tgr-1 impaired TLCV movement, raising the possibility that it may specify a host factor essential for viral systematic infection. This makes tgr-1 a strong candidate for developing resistance in major crops carrying the gene homologue.  相似文献   

Predicting perchlorate exposure in milk from concentrations in dairy feed     

Rice CP  Baldwin Vi RL  Abbott LC  Hapeman CJ  Capuco AV  Le A  Bialek-Kalinski K  Bannerman DD  Hare WR  Paape MJ  McCarty GW  Kauf AC  Sadeghi AM  Starr JL  McConnell LL  Van Tassell CP 《Journal of agricultural and food chemistry》2007,55(21):8806-8813

Perchlorate has been detected in U.S. milk samples from many different states. Applying data from a recently reported 9-week experiment in which 16 Holstein dairy cows were administered perchlorate allowed us to derive an equation for the dose-response relationship between perchlorate concentrations in feed/drinking water and its appearance in milk. Examination of background concentrations of perchlorate in the total mixed ration (TMR) fed in addition to the variable dose supplied to treated cows as a ruminal infusate revealed that cows receive significant and variable exposure to perchlorate from the TMR. Weekly examination of the TMR disclosed that a change in ingredients midway through the experiment caused a significant (78%) change in TMR perchlorate concentration. Analyses of the ingredients comprising the TMR revealed that 41.9% of the perchlorate came from corn silage, 22.9% came from alfalfa hay and 11.7% was supplied by sudan grass. Finally, USDA Food and Nutrition Survey data on fluid milk consumption were used to predict potential human exposure from milk that contained concentrations of perchlorate observed in our previous dosing study. The study suggests that reducing perchlorate concentration in dairy feed may reduce perchlorate concentrations in milk as well as the potential to reduce human exposure to perchlorate in milk.  相似文献   

Influence of flavonols as in vitro on low density lipoprotein glycation     

Ghaffari MA  Mojab S 《Iranian Biomedical Journal》2007,11(3):185-191

INTRODUCTION: The non-enzymatic glycation of Low density lipoprotein (LDL) is a naturally occurring chemical modification of apolipoprotein B as a result of condensation between lysine residues and glucose. Glycated LDL is poorly recognized by LDL receptors and initiates different processes that can be considered proatherogenic. Thus, LDL glycation may contribute in the increased atherosclerotic risk of patients with diabetes. The objective of this study was to investigate the effect of naturally occurring flavonols on LDL glycation in vitro. METHODS: In this study, LDL was isolated from EDTA-plasma by ultracentrifugation using a single step discontinuous gradient. Then, glucose was added to LDL and LDL glycation level was estimated in absence and presence of flavonols by sodium periodate assay. RESULTS: This study was showed that five flavonols: quercetin, myricetin, kaempferol, rutin and morin decreased LDL glycation in a dose-dependent manner. Also, it was demonstrated this nutrients decreased electrophoretic mobility of glycated LDL. CONCLUSION: The results of this investigation show that flavonols probably with their antioxidant properties inhibited LDL glycation and thus may have a role in ameliorating atherosclerotic risk of patients with diabetes mellitus.  相似文献   

Antibody seroprevalences against Peste des Petits Ruminants (PPR) virus in sheep and goats in Sudan     

Nussieba A. Osman  A. S. Ali  Mahasin E. A/Rahman  M. A. Fadol 《Tropical animal health and production》2009,41(7):1449-1453

Counter immnuo-electrophoresis (CIEP) and Competitive ELISA (C-ELISA) tests were employed for seroprevalence of Peste des Petits Ruminants (PPR) infection in Sudan. The result of both tests showed high prevalence of PPRV antibodies in sheep and goats sera collected from six different regions of Sudan. Of the 519 serum samples examined for the presence of PPRV antibodies 307(59.15%) were positive by CIEP while 263(50.67%) were positive by C-ELISA. CIEP technique was shown to be more sensitive than C-ELISA technique for detection of PPRV antibodies (Kappa statistics 0.259). C-ELISA allowed rapid, simple, specific, sensitive and differential sero-diagnosis of PPRV and RPV in sheep, goats and cattle. CIEP is, unlike competitive ELISA, is group-specific test and can not differentiate between PPR and RP infections. Despite its low specificity CIEP can be a useful indicative screening test for PPRV antibodies in flocks that neither been vaccinated nor otherwise exposed to PPR or RP virus. Results obtained suggest that CIEP, like the HI test, could be a useful screening test where it is not possible to use C-ELISA.  相似文献   

Peste des petits ruminants virus (PPRV) infection; Its association with species,seasonal variations and geography     

Muhammad Abubakar  Syed Muhammad Jamal  Muhammad Javed Arshed  Manzoor Hussain  Qurban Ali 《Tropical animal health and production》2009,41(7):1197-1202

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Exclusion of NEU1 and PPGB from candidate genes for a lysosomal storage disease in Japanese Black cattle     

Ali Akbar MASOUDI  Osamu YAMATO  Kazuhiro YONEDA  Takehito TSUJI  Osamu MIKAMI  Tetsuo KUNIEDA 《Animal Science Journal》2009,80(5):611-615

A case of lysosomal storage disease has been reported in a calf of Japanese Black cattle. Lysosomal storage diseases are hereditary diseases caused by deficiency of lysosomal hydrolases. The clinical and pathological features and accumulated substrates of the affected animal indicated a possibility of sialidosis or galactosialidosis caused by deficiency of neuraminidase (NEU1) or protective protein for β-galactosidase (PPGB). In the present study, we investigated nucleotide sequences of the genes encoding these two proteins to evaluate whether mutation of these genes is involved in this disease. We determined cattle genomic sequences of these two genes by using bovine EST sequences and the nucleotide sequences of all exons of these genes were compared between affected and normal animals. The results showed several nucleotide substitutions, but none of them was a functional mutation or specific to the affected animal. Furthermore, genotyping of the microsatellite markers in the vicinity of these two genes revealed no homozygosity of the chromosomal regions including these genes in the affected animal. These findings indicated that neither NEU1 nor PPGB gene is responsible for the lysosomal storage disease of Japanese Black cattle and therefore the disease is neither sialidosis nor galactosialidosis.  相似文献   

Establishing an efficient in vitro propagation system for sweet cherry rootstocks Gisela 12 and Maxma 14 and assessment of genetic homogeneity by ISSR markers     

Jafarlou  Ali Mehrban  Pirivatlo  Saeed Piri  Salehi  Behrouz  Mogbli  Amir Hoshang Hoseynzadeh 《Journal of Crop Science and Biotechnology》2021,24(4):449-460

Journal of Crop Science and Biotechnology - This survey is aimed at the assessment of the possible effect of different plant growth regulators (PGRs) on the micropropagation of commercial sweet...  相似文献