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JA Ruben Dal Sasso C NR Geist WJ Hillenius TD Jones M Signore 《Science (New York, N.Y.)》1999,283(5401):514-516
Ultraviolet light analysis of a fossil of the theropod dinosaur Scipionyx samniticus revealed that the liver subdivided the visceral cavity into distinct anterior pleuropericardial and posterior abdominal regions. In addition, Scipionyx apparently had diaphragmatic musculature and a dorsally attached posterior colon. These features provide evidence that diaphragm-assisted lung ventilation was present in theropods and that these dinosaurs may have used a pattern of exercise physiology unlike that in any group of living tetrapods. 相似文献
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Generation of embryonic stem‐like cells from in vivo‐derived porcine blastocysts at a low concentration of basic fibroblast growth factor 
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下载免费PDF全文 NR Han H‐Y Kim S Baek S‐H Lee J Lee E Lee C‐K Park ST Lee 《Reproduction in domestic animals》2018,53(1):176-185
Although basic fibroblast growth factor (bFGF) is an essential factor supporting the maintenance of porcine embryonic stem (ES) cell self‐renewal and pluripotency, its high cost has limited previous studies, and the development of a low‐cost culture system is required. For these systems, in vivo blastocysts were progressively cultured under various conditions consisting of different culture mediums and/or different feeder cell numbers at a low concentration of bFGF. As the results, the sequential culture of in vivo‐derived porcine blastocysts on 5.0 × 105 mouse embryonic fibroblast (MEF) feeder cells in alpha minimum essential medium‐based medium for primary culture, on 2.5 × 105 MEF feeder cells in Mixture medium for the 1st subpassage, and on 2.5 × 105 MEF feeder cells in DMEM/Ham's F10‐based medium for the post‐2nd subpassage could support the establishment and maintenance of porcine ES‐like cells at the low concentration of bFGF. The established porcine ES‐like cells showed ES cell‐specific characteristics such as self‐renewal and pluripotency. We confirmed that porcine ES‐like cells could be generated from in vivo‐derived porcine blastocysts at a low concentration of bFGF. 相似文献
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The concentrations of cadmium (Cd), lead (Pb), chromium (Cr), copper (Cu), zinc (Zn), and iron (Fe) in water and selected fish species (Oreochromis nilzinc (oticus and Clarias gariepinus) from Benue-Niger River Confluence, Nigeria were investigated. The water and fish sample digests were quantified using an atomic absorption spectrophotometer. The study aimed to assess any possible human health risk associated with water and fish consumption from the selected geographical area. The concentration of the metals in the surface water ranged from 0.007 to 0.039, 0.106 to 0.240, 0.092 to 0.0164, 0.682 to 0.878, 0.058 to 0.309, and 0.136 to 1.645 mg/L for Cd, Cr, Cu, Fe, Pb, and Zn, respectively, while the concentrations of the metals, Cd, Pb, Cr, Cu, Zn, and Fe in these fish species ranged from 0.034 to 0.679, 0.500 to 1.75, 0.152 to 0.351, 0.36 to 1.01, 5.32 to 7.41, and 4.25 to 9.17 mg/kg, respectively. Cd, Cr, and Pb levels in fish were higher than the provisional tolerable weekly intake of 7, 1.5, and 25 μg/kg bw, respectively, according to the World Health Organization. Dry season values of the metals in water samples were higher, and sharptooth catfish had higher amounts of the metals. The hazard quotient (HQ) for Cd indicated a possible potential health concern with fish consumption from this area. 相似文献
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Comparison of the effects of topical application of UMF20 and UMF5 manuka honey with a generic multifloral honey on wound healing variables in an uncontaminated surgical equine distal limb wound model 下载免费PDF全文
下载免费PDF全文 AS Tsang AJ Dart A Sole‐Guitart CM Dart NR Perkins LB Jeffcott 《Australian veterinary journal》2017,95(9):333-337