Engineering yeast transcription machinery for improved ethanol tolerance and production     

Alper H  Moxley J  Nevoigt E  Fink GR  Stephanopoulos G 《Science (New York, N.Y.)》2006,314(5805):1565-1568

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Evaluation of IGF-I levels and serum protein profiles of diabetic cats and dogs     

Gulay Ciftci  Gul Fatma Yarim 《Journal of veterinary science (Suw?n-si, Korea)》2011,12(4):325-331

In this study, we measured the insulin-like growth factor (IGF)-I levels and evaluated the serum protein profiles of diabetic, insulin-treated, and healthy cats and dogs. The total IGF-I concentrations were 33.74 ± 3.4 ng/mL for normal, 25.8 ± 4.5 ng/mL for diabetic, and 180.4 ± 31.4 ng/mL for insulin-treated cats. IGF-I concentrations were 46.4 ± 6.6 ng/mL for normal, 25.1 ± 4.1 ng/mL for diabetic, and 303.0 ± 61.3 ng/mL for insulin-treated dogs. Total serum protein profiles were analyzed by SDS-PAGE. Fourteen bands ranging from 25 to 240 kDa in size were observed for cats, and 17 bands ranging from 25 to 289 kDa were observed for dogs. The densities of the bands differed among control, diabetic, and insulin-treated animals. In conclusion, we found that serum protein profiles and IGF-I concentrations were altered in both diabetic and insulin-treated animals. When judiciously interpreted in the light of other clinical and laboratory data, the techniques used in our study provide a valuable modality for measuring the severity of diabetes mellitus in dogs and cats.  相似文献   

The microchip microbe hunters     

J Alper 《Science (New York, N.Y.)》1990,247(4944):804-806

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Archimedes, Plato Make Millions for Big Oil!: A University of Chicago geochemist draws on the Classics to understand zeolite structure     

Alper J 《Science (New York, N.Y.)》1990,248(4960):1190-1191

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Batteries. Inside a battery     

Alper J 《Science (New York, N.Y.)》2002,296(5571):1225

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Batteries. The battery: not yet a terminal case     

Alper J 《Science (New York, N.Y.)》2002,296(5571):1224-1226

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Protein structure. Stretching the limits     

Alper J 《Science (New York, N.Y.)》2002,297(5580):329-331

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Evaluation of PCR methods for detection of <Emphasis Type="Italic">Brucella</Emphasis> strains from culture and tissues     

Alper Çiftci  Tuba İça  Serap Savaşan  Barış Sareyyüpoğlu  Mehmet Akan  Kadir Serdar Diker 《Tropical animal health and production》2017,49(4):755-763

The genus Brucella causes significant economic losses due to infertility, abortion, stillbirth or weak calves, and neonatal mortality in livestock. Brucellosis is still a zoonosis of public health importance worldwide. The study was aimed to optimize and evaluate PCR assays used for the diagnosis of Brucella infections. For this aim, several primers and PCR protocols were performed and compared with Brucella cultures and biological material inoculated with Brucella. In PCR assays, genus- or species-specific oligonucleotide primers derived from 16S rRNA sequences (F4/R2, Ba148/928, IS711, BruP6-P7) and OMPs (JPF/JPR, 31ter/sd) of Brucella were used. All primers except for BruP6-P7 detected the DNA from reference Brucella strains and field isolates. In spiked blood, milk, and semen samples, F4-R2 primer-oriented PCR assays detected minimal numbers of Brucella. In spiked serum and fetal stomach content, Ba148/928 primer-oriented PCR assays detected minimal numbers of Brucella. Field samples collected from sheep and cattle were examined by bacteriological methods and optimized PCR assays. Overall, sensitivity of PCR assays was found superior to conventional bacteriological isolation. Brucella DNA was detected in 35.1, 1.1, 24.8, 5.0, and 8.0% of aborted fetus, blood, milk, semen, and serum samples by PCR assays, respectively. In conclusion, PCR assay in optimized conditions was found to be valuable in sensitive and specific detection of Brucella infections of animals.  相似文献   

Assessment of spatial and seasonal changes in groundwater nitrate pollution of agricultural lands through ordinary and indicator kriging techniques     

Hakan Arslan  Nazlı Ayyıldız Turan  Yusuf Demir  Alper Güngör  Bilal Cemek 《Archives of Agronomy and Soil Science》2017,63(7):907-917

Geostatistical approaches (ordinary kriging (OK) and indicator kriging (IK)) were used in this study to investigate the spatial and temporal variations in groundwater nitrate concentrations in Çar?amba plain of Turkey. Groundwater samples were taken in April 2012, July 2012, September 2012 and March 2013 from 78 groundwater wells. The experimental semivariograms were often fitted well by a Gauss model for April 2012 and September 2012, whereas a spherical model was fitted to experimental semivariograms for July 2012 and March 2013. Spatial distribution maps revealed that groundwater nitrate concentrations were above the threshold value of 50 mg L^?1 specified for drinking water in 4.3% of the study area in April 2012, 40.8% in July 2012, 32.8% in September 2012 and 19.1% in March 2013. Probability maps created with IK showed that 3.1% and 3.2% of the total area had very strong probability (0.8–1.0) of exceeding the threshold nitrate concentration in July 2012 and September 2012, respectively. Current findings revealed that groundwater nitrate concentrations changed seasonally and increased much more in summer. It was concluded that OK and IK may yield significant outcomes for groundwater management, identification of risky sites for potential pollution and identification of the sites with excessive fertilizer uses.  相似文献   

Revealing of resistant sources in <Emphasis Type="Italic">Cicer</Emphasis> species to chickpea leaf miner, <Emphasis Type="Italic">Liriomyza cicerina</Emphasis> (Rondani)     

Fatma Oncu Ceylan  Hatice Sari  Duygu Sari  Alper Adak  Fedai Erler  Cengiz Toker 《Phytoparasitica》2018,46(5):635-643

The chickpea leaf miner, Liriomyza cicerina (Rondani) (Diptera: Agromyzidae), is an important pest of cultivated chickpea (Cicer arietinum L.). A 2-year field study was carried out to screen a total of 126 Cicer germplasm for resistance to the leaf miner during the 2012 and 2013 growing seasons. Resistance was evaluated using a visual scale of 1–9, where 1?=?highly resistant and 9?=?very highly susceptible under natural infestation conditions. The results showed that two C. arietinum accessions, ILC 3397 and Sierra, had a score of 9 on the scale, being very highly susceptible. Three germplasm, one mutant (3304) and two breeding lines (LMR 140 and LMR 160) of C. arietinum, were found to be highly resistant with the scores ranging from 1.5 to 2 for resistance to the leaf miner. The mutant, 3304, was detected for the first time in this study as a highly leaf miner-resistant mutant of the cultivated chickpeas while the other two breeding lines had been previously reported as highly resistant against the leaf miner. In addition, two mutants and 14 breeding lines of C. arietinum and two mutants and one germplasm of C. reticulatum were identified as resistant having the scores from 2.1 to 3 on the 1–9 scale. The results suggest that these resistant germplasm may add a new dimension to chickpea breeding programs because they possess valuable traits for resistance against the pest. The resistant chickpeas that can be grown without using pesticides are important as environmental protection and reliable food source for human health.  相似文献