Dietary organic acids blend alone or in combination with an essential oil on the survival,growth, gut/liver structure and de hemato‐immunological in Nile tilapia Oreochromis niloticus     

Kennya Gomes Silva Addam  Scheila Anelise Pereira  Gabriel Fernandes Alves Jesus  Lucas Cardoso  Nichola Syracuse  Gustavo Ruschel Lopes  Nicollas Breda Lehmann  Bruno Correa da Silva  Lúvia Souza de S  Francisco Clio Maia Chaves  Maurício Latera Martins  Jos Luiz Pedreira Mourio 《Aquaculture Research》2019,50(10):2960-2971

This study evaluated the effects of dietary supplementation of organic acids blend (OAB) alone or in combination with essential oil, Lippia origanoides (OAE) for Nile tilapia fed supplemented diets for 30 days. Fish (1.1 ± 0.04 g) were fed control (Control), or OAB 0.5% or OAB 0.5% + essential oil 0.125% (OAE) respectively. At the end of the experiment, samples were collected for de hemato‐immunological, histological analysis of the intestine and liver, as well as microbiology of the intestine. The pH of the diets supplemented with OAB and OAE reduced 0.92 and 0.19 respectively. The growth and FCR were unaffected by the treatments, but survival was significantly higher in the OAB treatment. Fish fed the OAB diet showed reduced concentration of total heterotrophic bacteria and Pseudomonas sp. in the intestine. Increased glucose in fish fed OAB and high number of circulating monocytes in fish fed OAE diet were observed. The anterior intestine of fish fed OAE diet showed larger number of goblet cells and increased villi height. The diet supplemented with OAB, mainly, improved the intestinal health and survival of tilapia juveniles and can be used in juvenile production.  相似文献   

Effects of an Enterococcus faecium‐based probiotic on growth performance and health of Pirarucu,Arapaima gigas     

Natalino da Costa Sousa  Mrcia Valria Silva do Couto  Higo Andrade Abe  Peterson Emmanuel Guimares Paixo  Carlos Alberto Martins Cordeiro  Emilly Monteiro Lopes  Jonathan Stuart Ready  Gabriel Fernandes Alves Jesus  Mauricio Latera Martins  Jos Luiz Pereira Mourio  Paulo Csar Falanghe Carneiro  Alexandre Nizio Maria  Rodrigo Yudi Fujimoto 《Aquaculture Research》2019,50(12):3720-3728

Intensive fish farming has resulted in an increased concern for disease outbreaks. Probiotic use is one of the strategies being developed to improve fish health and productivity. Measures of probiotic colonization, growth performance, haematological characteristics and parasite load were used to evaluate the effect of diets supplemented with Enterococcus faecium on growth and health of Arapaima gigas juveniles. A completely randomized design with four treatments (diet with E. faecium at 1 × 10⁶ CFU/g and 1 × 10⁸ CFU/g, control diet and diet with the culture medium MRS) and three replicates was used. Ninety‐six Arapaima juveniles were distributed in 12 cages fed with the specified diet for 68 days. Colonization of the intestinal tract by lactic acid bacteria reduced the total number of heterotrophic bacteria in fish fed with probiotics compared to controls. Fish fed a supplemented diet containing 1 × 10⁸ CFU/g presented higher values of weight gain, survival and fish growth uniformity, and lower values of feed conversion ratio. The prevalence of Trichodina sp. could have affected the survival of fish in the control group. Reduction in parasite load and an increase in haematocrit, the number of erythrocytes, thrombocytes, neutrophils and monocytes were also observed in fish fed the diet containing 1 × 10⁸ CFU/g. Enterococcus faecium presented a probiotic effect in A. gigas juveniles and can be recommended for use at a concentration of 1 × 10⁸ CFU/g to modify the gut microbiota, improve growth performance and haematology and reduce parasitic load.  相似文献   

Characterization of the anthracnose resistance gene present in Ouro Negro (Honduras 35) common bean cultivar     

Ana Lilia Alzate-Marin  Marcia Regina Costa  Klever Marcio Arruda  Everaldo Gonçalves de Barros  Maurilio Alves Moreira 《Euphytica》2003,133(2):165-169

Ouro Negro (Honduras 35) is a highly productive Mesoamerican black seeded bean cultivar that possesses a major dominant gene conferring resistance to anthracnose (causal organism Colletotrichum lindemuthianum). In this work the anthracnose resistance gene present in Ouro Negro was characterized by studying allelic relationships to the following previously characterized anthracnose resistance genes (cultivars): Co-1 (MDRK), Co-1 ² (Kaboon), Co-1 ³ (Perry Marrow), Co-2 (Cornell 49-242), Co-3 (Mexico 222), Co-4 (TO), Co-4 ² (SEL 1308), Co-5 (SEL1360), Co-6 (AB 136), and the resistance genes present in PI 207262 and Widusa. In addition, we determined the resistance spectrum of Ouro Negro in relation to 19 pathotypes of C. lindemuthianum. The allelism tests confirmed that the dominant anthracnose resistance gene present in Ouro Negro is positioned at a locus distinct from those with which it was compared. We propose that this new gene be named Co-10. The inoculation of Ouro Negro with the 19 pathotypes of C. lindemuthianum demonstrated that Co-10 confers resistance to pathotypes 23, 64, 67, 73, 81, 83, 87, 89, 95, 102, 117, 119, 343, 453, 1033, 1545 and 1600. The identification of Co-10 is an important contribution to bean breeding programs that are in constant need of new sources of resistance to anthracnose. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

Improving the estimation of soil water evaporation based on days after wetting     

Élvis da Silva Alves  Lineu Neiva Rodrigues  Fernanda Laurinda Valadares Ferreira 《Journal of Agronomy and Crop Science》2023,209(1):176-187

Soil evaporation constitutes a major pathway of water loss in agriculture. Understanding its dynamics in the face of drying and soil cover is fundamental to improve both simulation models and the sustainability of production systems. Thus, the objective of this study was to estimate soil evaporation as a function of drying and percentage of soil cover. Three experiments were carried out in three different periods. In each of the periods, in parallel and in an adjacent area, an experiment was carried out to evaluate the influence of weighing micro-lysimeter (ML) height on the estimation of direct soil evaporation. The experiments were installed with a completely randomized design. For the experiments that measured evaporation as a function of cover, the treatments consisted of six different percentages of cover of the internal area of the ML (0%, 10%, 25%, 50%, 75% and 100%), with four replicates, using artificial plants to cover the soil. The experiments that estimated evaporation as a function of ML height were conducted with eight repetitions and three different ML heights (100, 200 and 300 mm). It was observed that the accumulated evaporation up to 25 days after wetting was 52% and 53% lower in micro-lysimeters with 100 mm height, compared to those with 200 and 300 mm height, respectively, for all experiments. The new model developed to calculate soil evaporation as a function of the days after wetting and percentage of soil cover showed excellent performance (NSE > 0.95).  相似文献   

Thermal comfort indices assessed in integrated production systems in the Brazilian savannah     

Caroline Carvalho de Oliveira  Fabiana Villa Alves  Roberto Giolo de Almeida  Érick Lemes Gamarra  Severino Delmar Junqueira Villela  Paulo Gustavo Macedo de Almeida Martins 《Agroforestry Systems》2018,92(6):1659-1672

The objective was to evaluate the animal thermal comfort indices from two integrated crop-livestock-forestry (ICLF) systems. For this, temperature–humidity index (THI), black globe temperature and humidity index (BGHI), and the radiant thermal load (RTL) were assessed. Two ICLF (ICLF-1 and ICLF-2) systems and one control system were established. On the ICLF systems, the arboreal component was the eucalyptus tree (Eucalyptus grandis × Eucalyptus urophylla; H13 clone), planted in simple wide-spaced rows. The ICLF-1 system had a tree spacing of 14 × 2 m with 357 trees per hectare, and the ICLF-2 had a tree spacing of 22 × 2 m with 227 trees per hectare. The control system had five scattered native trees per hectare, pertaining to Gochnatia and Dipteryx species. The forage component in all three systems was piatã-grass (Brachiaria brizantha cv. BRS Piatã). The experimental design was a randomized block in a sub-subplot design scheme with four replications. The presence of shade provided by the trees offered better conditions of animal comfort when compared with the condition of full sun. The ICLF-1 system, with higher tree density, provided better indicators for thermal comfort, THI, BGHI, and RTL when compared with the condition of full sun, while ICLF-2 was no different than ICLF-1 for BGHI.  相似文献   

Autochthonous probiotic as growth promoter and immunomodulator for Astyanax bimaculatus cultured in water recirculation system          下载免费PDF全文

Andressa Vieira de Moraes  Marina de Oliveira Pereira  Klayton Natan Moraes  Jorge P Rodrigues‐Soares  Gabriel Fernandes Alves Jesus  Adolfo Jatobá 《Aquaculture Research》2018,49(8):2808-2814

This work aimed to evaluate the probiotic Lactobacillus spp. in the microbiota of Astyanax bimaculatus reared in a water recirculation system. The experiment was randomized, using 800 postlarvae distributed into eight polyethylene containers, separated into two treatments, in quadruplicate: supplementation with Lactobacillus spp. (Probiotic) and no supplementation (Control). After 90 days of culture, 13 fish per container were anaesthetized. Three of these underwent microbiological evaluation, and haematological analyses were carried out on five. Immunological assessment was performed on another five fish. Zootechnical parameters were evaluated for all animals. In the microbiological evaluation, the probiotic group presented higher counts of lactic acid bacteria, but lower counts of Vibrio spp., Pseudomonas spp., and Staphylococcus spp., when compared with control. After haematological analysis, the supplemented group presented low haematimetric indices of Mean Corpuscular Haemoglobin Concentration and Mean Corpuscular Haemoglobin (MCHC and MCH, respectively) and higher amounts of total leucocytes, thrombocytes and circulating monocytes when compared with control. The immunological profile did not differ between treatments. In the zootechnical performance, probiotic group presented higher productivity, survival and apparent food efficiency when compared with control. Therefore, when Lactobacillus spp. was supplemented in the diet of A. bimaculatus, immunocompetence and the zootechnical performance of animals increased.  相似文献   

Availability of minerals in rendered meat and bone meal for Nile tilapia: Preliminary observations          下载免费PDF全文

L. B. de Moura  T. O. Xavier  D. A. V. Campelo  M. Michelato  F. L. Alves de Almeida  L. V. O. Vidal  W. M. Furuya 《Aquaculture Nutrition》2018,24(3):991-997

A digestibility trial was conducted with 180 Nile tilapia (32.65 ± 4.52 g) fed a reference diet containing 350 g/kg crude protein and 4,500 kcal/kg gross energy. Five different meat and bone meals (MBMs) were evaluated. The test diets compound by 70% of reference diet and 30% of each MBM were elaborated and classified according to ash content: MBM‐430, MBM‐400, MBM‐370, MBM‐340 and MBM‐310. The apparent availability coefficient (AAC) of minerals and the apparent digestibility coefficient (ADC) of dry matter (DM) were subjected to one‐way analysis of variance (p < .05). Differences among ADC and AAC were determined by Tukey's multiple range test (p < .05). When appropriate, a polynomial regression analysis was performed. Linear correlation analyses between MBM chemical composition and the AAC and ADC were performed (p < .01). Higher values of ADC_DM, AAC_Ca and AAC_P were observed in MBM‐310, while low values were observed in MBM‐430. Quadratic effects of ash, P and Ca contents on AAC_Ca, AAC_P and ADC_DM were observed. The AAC_Ca, AAC_P and ADC_DM indicated a negative correlation with ash, P and Ca contents. MBM‐310 exhibited the highest mineral AAC, which could be used as a mineral source for Nile tilapia.  相似文献   

Could the Supply of Boron and Zinc Improve Resistance of Potato to Early Blight?     

Patrícia Pereira Machado  Fábio Steiner  Alan Mario Zuffo  Rafael Alves Machado 《Potato Research》2018,61(2):169-182

The adequate supply of boron (B) and zinc (Zn) can improve the structural integrity and permeability of cell membranes, which is a defence mechanism against fungi infection. A greenhouse experiment was conducted to investigate the effect of the application of B and Zn on plant growth and the incidence and severity of potato early blight, a destructive foliar disease of potato crop caused by Alternaria grandis E.G. Simmons. Potato plants (Solanum tuberosum L. cv. Ágata) were grown in 11-l pots filled with sandy soil, unfertilized or fertilized with 5 mg kg^?1 B and 5 mg kg^?1 Zn (either alone or in combination). Potato plants were inoculated with isolates of Alternaria grandis at 40 days after planting. Early blight incidence and severity were evaluated visually 7 days after inoculation with A. grandis isolates. Disease incidence ranged from 16 to 41% infected leaves, and was significantly highest in the treatment with the application of B, followed by the control, and lowest with application of Zn and B + Zn. Early blight severity ranged from 2.5 to 25%, and was significantly higher in the unfertilized treatment than in those fertilized with Zn either alone or in combination with B. These findings suggest that Zn plays a critical role in potato tolerance to early blight and should be considered as a preventive measure in the disease management plan, since application of zinc reduced the incidence and severity of potato early blight. However, the physiological basis of this remains unknown.  相似文献   

Characterization of field‐evolved resistance to Bacillus thuringiensis‐derived Cry1F δ‐endotoxin in Spodoptera frugiperda populations from Argentina          下载免费PDF全文

Desmi I Chandrasena  Ana M Signorini  Gustavo Abratti  Nicholas P Storer  Magdalena L Olaciregui  Analiza P Alves  Clinton D Pilcher 《Pest management science》2018,74(3):746-754

BACKGROUND

Transgenic maize (Zea mays L.) event TC1507 (Herculex^® I insect protection), expressing Cry1F δ‐endotoxin derived from Bacillus thuringiensis var. aizawai, was commercialized in 2003 in the Americas. Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) susceptibility to Cry1F was monitored annually across several regions in Argentina using diagnostic concentration bioassays. Reduced performance of TC1507 maize against S. frugiperda was reported in 2013. A resistant population was established in the laboratory and the dominance of Cry1F resistance was characterized.

RESULTS

During 2012–2015, high‐survivorship of several populations was observed in the resistance monitoring program. Reciprocal crosses of a Cry1F‐resistant population with a Cry1F‐susceptible population were evaluated to calculate effective dominance (D_ML) based on mortality levels observed at 100 µg/ml Cry1F. Two additional dominance levels (D_LC and D_EC) were calculated using lethal (LC₅₀) or effective concentration (EC₅₀) derived from concentration–response bioassays. Estimates indicated that Cry1F resistance in S. frugiperda in Argentina was either highly recessive (D_ML = 0.005) or incompletely recessive (D_LC < 0.26 and D_EC < 0.19).

CONCLUSION

This study is the first documented confirmation and characterization of S. frugiperda Cry1F field‐evolved resistance in Argentina. The resistance to Cry1F in S. frugiperda populations collected in Argentina, is autosomal and incompletely recessive similar to the resistance reported in Brazil. © 2017 The Authors. Pest Management Science published by John Wiley © Sons Ltd on behalf of Society of Chemical Industry.  相似文献   

Sodium fluorescein as an internal tracer on the location of bovine urine patches in pastures          下载免费PDF全文

M. R. Gusmão  T. C. Alves  A. P. Lemes  G. M. Bettiol  A. de F. Pedroso  W. Barioni Junior  P. P. A. Oliveira  C. R. Grego 《Grass and Forage Science》2016,71(2):305-314

Sodium fluorescein (SF) was evaluated and validated as an internal marker in cattle for the location of urine patches in pastures. Three trials were carried out aiming at the following: evaluating the effect of dilution and volume of application on SF fluorescence on the environment; testing dosages and duration of excretion of SF administered orally to cattle; and validating the methodology for SF use on the location of cattle urine patches in pastures. The marker was tested in beef cattle kept on pastures under three grazing systems: degraded pasture under extensive management (De), intensively managed dryland pasture with high stocking rate (Id) and intensively managed irrigated pasture with high stocking rate (Ii). Besides the localization of urination sites, the number of urinations/animal/d and the area covered by urine were also determined. The residue of SF remained fluorescent in the pasture foliage up to 34 h after urination events, allowing the location of urine patches by a black‐light flashlight. There was no difference between grazing systems in the number of urine patches/animal/d during the rainy and dry seasons. The average number of urine patches was approximately 11/animal/d. As expected, the estimated volume and area covered by urine varied according to the stocking density. The chosen SF dose (50 mg kg^?1 LW) did not adversely affect the animals when administered once daily during 2 d. However, the same SF dose administered during four consecutive days caused urinary disorder in the animal. The distribution of urine patches was spatially dependent on specific characteristics of the paddock.  相似文献