苦荞麦毛状根的诱导研究     

孙雁霞  王跃华  陈燕  张红玉  赵钢  邬晓勇 《西北农业学报》2012,21(7):91-94

利用发根农杆菌Ri1601浸染苦荞植物预培养2d的叶片外植体,经不同时间的共培养和除菌培养后获得毛状根。经硅胶薄层色谱板检测表明,发根农杆菌Ri1601和苦荞叶片上未经转化产生的不定根都不含有冠瘿碱;而经发根农杆菌Ri1601遗传转化后所产生的毛状根有冠瘿碱的存在。因此,可确定苦荞叶片上产生的毛状根为发根农杆菌Ri1601转化所得。建立了用发根农杆菌Ri1601诱导苦荞植物叶片产生毛状根的有效方法。  相似文献   

豌豆品种晋豌5号在晋北地区的春播高产栽培技术   总被引：3，自引：0，他引：3  

刘飞  冯高  刘支平  陈燕妮  杨芳 《内蒙古农业科技》2012,(4):112

文章介绍了豌豆品种晋豌5号优质高产栽培技术,包括品种的主要特征特性、种子处理、施肥、播种、田间管理、病虫害防治、收获与贮藏等方面内容。  相似文献   

不同方法提取野生龙眼基因组DNA的效果比较     

刘锴栋  袁长春  黎海利  陈燕  刘金祥 《广东农业科学》2012,39(20):131-133

以广东、海南的3种野生龙眼幼嫩叶片为试验材料,采用常规SDS法、常规CTAB法、改良SDS法和改良2xCTAB法提取龙眼叶片基因组DNA,探索从富含单宁、多酚、多糖和色素等次生代谢物质的野生龙眼叶片中获得高质量DNA的提取方法.结果表明:采用改良的2xCTAB法提取的龙眼叶片基因组DNA纯度最高、质量最佳,可直接用于龙眼RAPD分析和叶绿体DNAtrnL-F基因的PCR扩增分析,且扩增后条带清晰.说明改良的2xCTAB方法获得的DNA纯度和产率较高,提取效果较好.  相似文献   

Research and development of the real time remote monitoring control system of hybrid electric vehicles     

YANG Ya Lian  ZHENG Yang  SONG An Xing  LU Tong 《保鲜与加工》2012,(6):1-8

This paper develops a vehicle carried remote data acquisition terminal based on GPS/GPRS integrated DTU(data transfer unit) and CAN Bus data acquisition module, and built a parameter remote monitoring system of HEV with GPRS and Internet. By using PIC18F4580 with built in CAN control module and industrial DTU MD610G, we transmitted the parameter data and GPS information of the HEV(Hybrid Electric Vehicle) terminals to the remote data control center through GPRS(General Packet Radio Service) and INTERNET. Taking VC++ as the development tool, we programmed a monitoring software of data exchanging, data processing and monitoring. The software also has the function of CANoe data playbacking and strengthened the system versatility. Eventually, the parameter remote monitoring system of HEV is developed. The experiment results show that the system works well.  相似文献   

微波辅助提取无花果多糖的工艺研究     

赵群  童军茂  田金虎  陈燕 《农产品加工．学刊》2012,(8):58-60,70

研究微波辅助提取无花果多糖的方法,通过单因素和正交试验确定无花果多糖的最佳提取条件。结果表明,无花果多糖的最佳提取工艺为:料液比为1∶50,浸泡时间为60 min,微波功率为640 W,微波时间为3 min。在该条件下,无花果多糖得率为4.65%。  相似文献   

Phenotypic characterization and random amplified polymorphic DNA (RAPD) analysis of Pasteurella multocida isolated from Korean pigs     

Lee KE  Jeoung HY  Lee JY  Lee MH  Choi HW  Chang KS  Oh YH  An DJ 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(5):567-573

Pasteurella multocida causes various respiratory disease symptoms in pigs, including atrophic rhinitis and pneumonia. In the present study, 69 strains of P. multocida were isolated from 443 pigs with respiratory clinical symptoms at 182 farms located throughout South Korea from 2009 to 2010. A multiplex capsular PCR typing assay revealed that 69 strains of P. multocida isolated in this study had the biosynthetic locus of the capsules of either serogroup A (47 strains, 68.1%) or serogroup D (22 strains, 31.9%). The 22 strains positive for serogroup D-specific primers were divided into four clusters and the 47 strains positive for serogroup A-specific primers were divided into 12 clusters according to the results of Random Amplified Polymorphic DNA (RAPD) analysis. P. multocida strains in the present study were susceptible to most of the antimicrobial agents used. An analysis of antimicrobial resistance and virulence gene pattern combined with RAPD indicated that a certain P. multocida strain appeared to be genetically identical, implying the persistence of the strain within a single farm.  相似文献   

Association of a missense mutation in the luteinizing hormone/choriogonadotropin receptor gene （LHCGR） with superovulation traits in Chinese Holstein heifers     

Yong Yu  Yunwei Pang  Haichao Zhao  Xiaoling Xu  ZhonghongWu Lei  An Jianhui  Tian 《畜牧与生物技术杂志(英文版)》2012,3(4):188-192

Background: Upon binding luteinizing hormone in the ovary, the luteinizing hormone/choriogonadotropin receptor (LHCGR) is necessary for follicular maturation and ovulation, as well as luteal function. We detected mutations in the LHCGR gene and evaluated their association with superovulation. Methods: Using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and DNA sequencing, we examined polymorphisms in LHCGR and the genotypes associated with superovulation traits in 127 Chinese Holstein heifers. Results: A G/T polymorphism (ss52050737) in exon 11 was significantly associated with the total number of ova and the number of transferable embryos. Conclusions: LHCGR may be a new predictor for superovulation in Chinese Holstein heifers.  相似文献   

益生菌制剂对早期断奶仔猪生长性能和免疫指标的影响   总被引：6，自引：0，他引：6  

刘虎传  张敏红  冯京海  姜海龙  杨晓岚  姜礼文  陈燕 《动物营养学报》2012,24(6):1124-1131

本试验旨在研究益生菌制剂对早期断奶仔猪生长性能和免疫指标的影响.选用48头平均体重为(6.39±0.42)kg的(21±2)日龄“杜×长×大”三元杂交断奶仔猪,按性别、体重和窝源基本一致的原则随机分为2组,每组4个重复,每个重复6头猪.试验1组为对照组,饲喂基础饲粮,试验2组为益生菌组,每kg基础饲粮中添加1×109 CFU活菌.试验期为21 d.结果表明:1)与对照组相比,添加益生菌制剂可显著提高断奶仔猪平均日增重(P＜0.05),并显著降低料重比和腹泻率(P＜0.05);2)与对照组相比,添加益生菌制剂可以显著提高仔猪断奶后第7天血清白介素-1β水平,显著提高断奶后第21天血清免疫球蛋白G含量、白介素-6和肿瘤坏死因子-α水平及回肠黏膜分泌性免疫球蛋白A含量(P＜0.05),空肠分泌性免疫球蛋白A含量稍高于对照组,但差异不显著(P＞0.05),粪便免疫球蛋白A含量及外周血T淋巴细胞CD3+、CD4+、CD8+及CD4+/CD8+无显著差异(P＞0.05).由结果可知,益生菌制剂可提高断奶仔猪的生长性能,增强早期断奶仔猪的免疫力,有效缓解早期断奶应激引起的仔猪免疫功能下降.  相似文献   

Seroprevalence of subtype H3 influenza A virus in South Korean cats     

Jeoung HY  Shin BH  Lee WH  Song DS  Choi YK  Jeong W  Song JY  An DJ 《Journal of Feline Medicine and Surgery》2012,14(10):746-750

To investigate the potential transmission of subtype H3 influenza virus to cats, a serological survey was carried out in South Korea. Serum samples (n = 1027) were obtained from 809 pet cats and 218 domesticated cats living in urban colonies (D-cats) from 2008 to 2010, and tested using an influenza anti-nucleoprotein (NP)-specific enzyme-linked immunosorbent assay (ELISA) and the haemagglutination inhibition (HI) test, which was recommended by the World Organization for Animal Health. Anti-influenza virus antibodies were detected in 3.12% and 2.43% of cat sera tested using the NP-specific ELISA and HI test, respectively. Anti-H3 antibodies were also identified when the HI assay was used for influenza virus serotyping. These data may indicate the sporadic transmission of subtype H3 influenza virus from other infected species to cats in South Korea.  相似文献   

水力侵蚀下砒砂岩区植被格局对微地形和侵蚀的影响     

朱志卓  李龙    张鹏  张尚轩  梁艳君  支杰  陈燕 《水土保持研究》2023,30(3):10-18,26

[目的]研究植被格局下微地形与侵蚀的关系,可为砒砂岩地区坡面侵蚀规律研究和水土流失防治提供理论参考。[方法]以砒砂岩区鲍家沟流域内的3种植被格局(均匀分布、聚集分布、随机分布)坡面为研究对象,通过自然降雨条件下采用野外径流小区实测结合三维激光扫描技术,分析了植被格局对微地形和侵蚀的影响。[结果]降雨后不同植被格局坡面的产流量、产沙量依次为裸坡坡面>聚集分布坡面>随机分布坡面>均匀分布坡面,与裸坡相比,均匀分布坡面的产流量、产沙量分别减少了64%,75%,其径流泥沙的拟合函数为Y=0.3619x^0.7309,R²=0.9866;不同植被格局坡面侵蚀—沉积的空间分布存在一定差异,与其他坡面相比,均匀分布格局坡面的土壤侵蚀强度最弱,侵蚀区面积最小,为12.38 m²,沉积区面积最大,为3.44 m²。不同植被格局坡面地形因子均随降雨呈现增大的趋势,且与裸坡坡面相比,聚集分布坡面地表粗糙度、地表起伏度、地表切割度、微坡度增幅最小,分别为5%,2%,0.5%,9%。聚集分布坡面地表粗糙度与产...  相似文献