Insecticide resistance status in the major West Nile virus vector Culex pipiens from Greece     

Ilias Kioulos  Anastasia Kampouraki  Evangelia Morou  George Skavdis  John Vontas 《Pest management science》2014,70(4):623-627

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Endocrine and ovarian responses in water buffalo cows immunized against inhibin and subjected to the Ovsynch protocol     

Abdalla Bahareldin-Ali  QIN Guang-sheng  GUO Ri-hong  Anastasia Tsigkou  TAN Zheng-zhun  HUANG Jian  LI Hui  LI Hui  SHI Zhen-dan 《农业科学学报》2015,14(9):1827-1837

The aim of this study was to investigate the feasibility of stimulating ovarian follicle development in order to improve fertility in water buffalo cows by immunization against inhibin. The experiment was carried out in early summer(May) and included 24 multi-parity crossbred Murrah-Swamp buffaloes that were divided into immunized(n=11) and control(n=13) groups. Each immunized cow was administered with a 2-m L immunogen of mineral oil adjuvant containing 2 mg of recombinant inhibin α-subunit fusion protein. The controls were treated with the adjuvant only. All animals received Ovsynch protocol treatment, starting on the day of the antigen administration, and they were artificially inseminated upon behavioral estrus. As a result, all of the immunized buffaloes generated antibodies against inhibin during the experimental period and had higher plasma concentrations of follicle-stimulating hormone(FSH), activin, and estradiol(E2) related to estrous expression. A higher proportion of immunized animals expressed estrus behavior than did the controls(72% vs. 30%, P0.05). On average, inhibin-immunized buffaloes had significantly more large follicles(≥9 mm in diameter) than the controls(mean±SEM; 1.2±0.1 vs. 0.84±0.1, respectively; P0.05) and a slightly higher mean total number of follicles(≥2 mm; 11.4±0.7 vs. 9.0±1.1, respectively; P=0.09) and small(2–4 mm) follicles(8.81±0.6 vs. 6.84±1.0, respectively; P=0.12). A higher percentage of cows ovulated in the immunized group than in the control group(91%(10/11) vs. 54%(7/13), respectively; P0.05). Moreover, inhibin-immunized cows had slightly larger corpus luteum(CL) than the controls 9 days after ovulation and significantly higher(P0.01) post-ovulation peak plasma progesterone(P4) concentrations. Immunization against inhibin also marginally increased the conception rate 42 days after insemination(45.8% vs. 15.4%; P0.05). These results demonstrate that immunization against inhibin, coupled with the treatment with the Ovsynch protocol, can constitute a new technique to increase fertility in water buffalo cows.  相似文献   

Evolution of imidacloprid resistance in Myzus persicae in Greece and susceptibility data for spirotetramat          下载免费PDF全文

Costas Ch Voudouris  Martin S Williamson  Panagiotis J Skouras  Amalia N Kati  Anastasia J Sahinoglou  John T Margaritopoulos 《Pest management science》2017,73(9):1804-1812

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Photocatalytic Degradation of Lindane in Aqueous Solution     

Anastasia Hiskia  Athanasios Mylonas  Despina Tsipi  Elias Papaconstantinou 《Pest management science》1997,50(2):171-174

Photolysis of lindane in aqueous solution, using near-visible and UV light (λ >320 nm) and in the presence of the polyoxometallate PW₁₂O₄₀^3-, results in its conversion to CO₂ and HCl. Initial photodecomposition takes place within a few minutes both in the presence and absence of dioxygen. The effective mineralization in the absence of dioxygen suggests that OH radicals act as the primary oxidant in this case.  相似文献   

A Collagen Basketweave from the Giant Squid Mantle as a Robust Scaffold for Tissue Engineering     

Anastasia Frolova  Nadezhda Aksenova  Ivan Novikov  Aitsana Maslakova  Elvira Gafarova  Yuri Efremov  Polina Bikmulina  Vadim Elagin  Elena Istranova  Alexandr Kurkov  Anatoly Shekhter  Svetlana Kotova  Elena Zagaynova  Peter Timashev 《Marine drugs》2021,19(12)

The growing applications of tissue engineering technologies warrant the search and development of biocompatible materials with an appropriate strength and elastic moduli. Here, we have extensively studied a collagenous membrane (GSCM) separated from the mantle of the Giant squid Dosidicus Gigas in order to test its potential applicability in regenerative medicine. To establish the composition and structure of the studied material, we analyzed the GSCM by a variety of techniques, including amino acid analysis, SDS-PAGE, and FTIR. It has been shown that collagen is a main component of the GSCM. The morphology study by different microscopic techniques from nano- to microscale revealed a peculiar packing of collagen fibers forming laminae oriented at 60–90 degrees in respect to each other, which, in turn, formed layers with the thickness of several microns (a basketweave motif). The macro- and micromechanical studies showed high values of the Young’s modulus and tensile strength. No significant cytotoxicity of the studied material was found by the cytotoxicity assay. Thus, the GSCM consists of a reinforced collagen network, has high mechanical characteristics, and is non-toxic, which makes it a good candidate for the creation of a scaffold material for tissue engineering.  相似文献   

Antiparasitic Effects of Sulfated Polysaccharides from Marine Hydrobionts     

Natalya N. Besednova  Tatyana S. Zaporozhets  Boris G. Andryukov  Sergey P. Kryzhanovsky  Svetlana P. Ermakova  Tatyana A. Kuznetsova  Anastasia N. Voronova  Mikhail Y. Shchelkanov 《Marine drugs》2021,19(11)

This review presents materials characterizing sulfated polysaccharides (SPS) of marine hydrobionts (algae and invertebrates) as potential means for the prevention and treatment of protozoa and helminthiasis. The authors have summarized the literature on the pathogenetic targets of protozoa on the host cells and on the antiparasitic potential of polysaccharides from red, brown and green algae as well as certain marine invertebrates. Information about the mechanisms of action of these unique compounds in diseases caused by protozoa has also been summarized. SPS is distinguished by high antiparasitic activity, good solubility and an almost complete absence of toxicity. In the long term, this allows for the consideration of these compounds as effective and attractive candidates on which to base drugs, biologically active food additives and functional food products with antiparasitic activity.  相似文献   

Effects of short photoperiod on energy intake,thermogenesis, and reproduction in desert hamsters (Phodopus roborovskii)          下载免费PDF全文

Xueying ZHANG  Zhijun ZHAO  Nina VASILIEVA  Anastasia KHRUSHCHOVA  Dehua WANG 《Integrative zoology》2015,10(2):207-215

Desert hamsters (Phodopus roborovskii) are the least known species in the genus Phodopus with respect to ecology and physiology, and deserve scientific attention, particularly because of their small body size. Here, the responses of energy metabolism and reproductive function to short photoperiods in desert hamsters were investigated. Male and female desert hamsters were acclimated to either long day (LD) (L:D 16:8 h) or short day (SD) photoperiods (L:D 8:16 h) for three months, and then the females were transferred back to an LD photoperiod for a further five months, while at the end of the SD acclimation the males were killed and measurements were taken for serum leptin as well as molecular markers for thermogenesis. We found that like the other two species from the genus Phodopus, the desert hamsters under SD decreased body mass, increased adaptive thermogenesis as indicated by elevated mitochondrial protein content and uncoupling protein‐1 content in brown adipose tissue, and suppressed reproduction compared to those under LD. However, different from the other two species, desert hamsters did not show any differences in energy intake or serum leptin concentration between LD and SD. These data suggest that different species from the same genus respond in different ways to the environmental signals, and the desert adapted species are not as sensitive to change in photoperiod as the other two species.  相似文献   

Antimicrobial properties of Quercus ilex L. proanthocyanidin dimers and simple phenolics: evaluation of their synergistic activity with conventional antimicrobials and prediction of their pharmacokinetic profile     

Karioti A  Sokovic M  Ciric A  Koukoulitsa C  Bilia AR  Skaltsa H 《Journal of agricultural and food chemistry》2011,59(12):6412-6422

The antibacterial and antifungal activities of an ample number of phenolic compounds isolated from Quercus ilex leaves, belonging to the classes of flavonoids, proanthocyanidins, and phenolic acids, are discussed. The isolation of A type proanthocyanidin, (+)-epigallocatechin-(2β→O→7, 4β→8)-(+)-catechin is reported for the first time. Its structure was established by means of highfield NMR (correlation spectroscopy, heteronuclear single quantum correlation, heteronuclear multiple bond correlation, and rotating frame Overhauser effect spectroscopy) and MS spectral analyses, while its absolute configuration was determined by circular dichroism measurements. The isolated compounds were tested for their antimicrobial effects against eight human bacterial species and 14 fungal species. In a second step, the most potent compounds were tested in combination with the conventional fungicides, bifonazole and ketoconazole, to evaluate possible synergistic effects. Results showed that proanthocyanidins 3 and 4 when combined with bifonazole and ketoconazole increase the activity of both of these conventional fungicides. Moreover, the pharmacokinetic profile of the isolated compounds was investigated using computational methods.  相似文献   

Phylogenetic relationship and variation of alarm call traits of populations of red‐cheeked ground squirrels (Spermophilus erythrogenys sensu lato) suggest taxonomic delineation     

Vera A. MATROSOVA  Anastasia D. IVANOVA  Elena V. VOLODINA  Ilya A. VOLODIN  Dmitry Yu. ALEXANDROV  Olga V. SIBIRYAKOVA  Oleg A. ERMAKOV 《Integrative zoology》2019,14(4):341-353

Distribution area and taxonomic borders within the species complex Spermophilus erythrogenys sensu lato remain questionable. Early evidence suggests that red‐cheeked ground squirrels of Southeast Kazakhstan are remarkably different in terms of the acoustic structure of their alarm calls from the red‐cheeked ground squirrels of the Kurgan region in Russia. In this study, we analyzed the differences in the acoustic structure of the alarm call and mitochondrial DNA (complete control region, 1005–1006 bp and complete cytochrome b gene, 1140 bp) in 3 populations of red‐cheeked ground squirrels (Tara, Altyn‐Emel and Balkhash), all located within areas isolated by geographical barriers in Southeast Kazakhstan. We found that the alarm call variables were similar between the 3 study populations and differed by the maximum fundamental frequency (8.46 ± 0.75 kHz) from the values (5.62 ± 0.06 kHz) reported for the red‐cheeked ground squirrels from the Kurgan region of Russia. Variation in mtDNA control region was only 3% and variation in cytochrome b gene was only 2.5%. Phylogenetic trees based on cytochrome b gene polymorphism of 44 individuals from the study area and adjacent territories indicated 3 clades with high (98–100%) bootstrap support: “intermedius,” “brevicauda” and “iliensis”). We conclude that the 3 study populations in Southeast Kazakhstan belong to the clade intermedius and suggest a taxonomical revision of the species complex Spermophilus erythrogenys sensu lato, including analyses of nuclear DNA and alarm calls for populations of the brevicauda and iliensis clades.  相似文献   

A survey of seasonal temperatures and vineyard altitude influences on 2-methoxy-3-isobutylpyrazine, C13-norisoprenoids, and the sensory profile of Brazilian Cabernet Sauvignon wines     

Falcão LD  de Revel G  Perello MC  Moutsiou A  Zanus MC  Bordignon-Luiz MT 《Journal of agricultural and food chemistry》2007,55(9):3605-3612

This report has investigated the seasonal temperatures influences (winter and summer) of five vineyards at different altitudes on the concentrations of 2-methoxy-3-isobutylpyrazine (MIBP), alpha- and beta-ionone, and beta-damascenone in 2004 or 2005 vintages of Cabernet Sauvignon wines from Santa Catarina State, Brazil. Sensorial analyses were also carried out on the wine samples and compared to altitude and climate. Significant regression was observed between MIBP concentrations and the vineyard's altitude. No significant relation was observed between alpha- and beta-ionone and beta-damascenone with the vineyard's altitude. Principal component analysis positively correlated wines from higher altitudes with a "bell pepper" aroma. Conversely, the wines made with grapes from lower altitudes were correlated with "red fruits" and "jam" aromas. An important relation between the bell pepper aroma and the lower winter temperature was observed. A strong negative correlation was also observed between seasonal temperatures and vineyard altitude as well as between MIBP content and seasonal temperature of growing grapevines.  相似文献