Patterns of gene expression during Drosophila mesoderm development     

Furlong EE  Andersen EC  Null B  White KP  Scott MP 《Science (New York, N.Y.)》2001,293(5535):1629-1633

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Another phocine distemper outbreak in Europe     

Jensen T  van de Bildt M  Dietz HH  Andersen TH  Hammer AS  Kuiken T  Osterhaus A 《Science (New York, N.Y.)》2002,297(5579):209

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Astrophysics goes South     

Andersen PH 《Science (New York, N.Y.)》1991,252(5012):1494-1495

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Bioaccumulation of melamine in catfish muscle following continuous, low-dose, oral administration     

Andersen WC  Turnipseed SB  Karbiwnyk CM  Evans E  Hasbrouck N  Mayer TD  Gieseker CM  Nochetto C  Stine CB  Reimschuessel R 《Journal of agricultural and food chemistry》2011,59(7):3111-3117

In this study, catfish muscle was analyzed for melamine (MEL) and cyanuric acid (CYA) residues following experimental feeding with low doses of MEL and MEL and CYA (MEL+CYA) and with the insoluble melamine-cyanurate complex (MEL=CYA). Catfish were daily fed 0.1 mg/kg BW of MEL for 15, 28, or 42 days, 0.1 mg/kg BW of MEL+CYA for 28 days, 2.5 mg/kg BW of MEL+CYA for 14 days, or 400 mg/kg BW of MEL=CYA for 3 days. Residues in the tissue were determined by LC-MS/MS. MEL was extracted with acidic acetonitrile, followed by defatting with dichloromethane, and isolated with cation exchange solid phase extraction (SPE). For CYA analysis, fish were extracted with dilute acetic acid, defatted with hexane, and cleaned up with a graphitic carbon SPE. Catfish fed 0.1 mg/kg BW of MEL reached a maximum muscle residue concentration of 0.33 ± 0.04 mg/kg (ppm) after 28 days of continuous feeding. The same concentration was found for MEL+CYA feeding at the 0.1 mg/kg BW level for 28 days. Feeding at 2.5 mg/kg BW of MEL+CYA yielded muscle concentrations above the 2.5 mg/kg level of concern for most of the study fish. Finally, catfish fed high levels of the MEL=CYA complex (400 mg/kg BW) accumulated relatively little MEL in the muscle (0.14 ± 0.07 mg/kg) and, unlike treatment with MEL+CYA, did not form renal melamine-cyanurate crystals. Appreciable concentrations of CYA were not detected in any of the muscles tested. These studies provide data to model the bioaccumulation of triazine residues into edible fish tissue as a result of the continuous consumption of adulterated feed.  相似文献   

Ascorbic acid and ascorbic acid 6-palmitate induced oxidation in egg yolk dispersions     

Nielsen JH  Kristiansen GH  Andersen HJ 《Journal of agricultural and food chemistry》2000,48(5):1564-1568

The oxidation in aqueous dispersions of egg yolk powder and the influence of addition of the proposed antioxidants ascorbic acid and ascorbic acid 6-palmitate indicate that both ascorbic acid and ascorbic acid 6-palmitate propagated the oxidation of egg yolk powder dispersions. Ascorbic acid 6-palmitate was found to be more prooxidative than ascorbic acid. Moreover, it was found that addition of ascorbic acid or ascorbic acid 6-palmitate gave rise to an increase in the amount of free iron Fe(II) in the egg yolk dispersions. It is proposed that ascorbic acid and ascorbic acid 6-palmitate react with the phosvitin-Fe(III) complex found in egg yolk and release Fe(II), which subsequently propagates lipid oxidation. It appears that less oxidation occurs in egg yolk dispersions exposed to high concentrations of peroxy radicals with added ascorbic acid than egg yolk dispersions with added ascorbic acid without exposure to peroxy radicals.  相似文献   

Distribution and fractionation of heavy metals in pairs of arable and afforested soils in Denmark   总被引：1，自引：0，他引：1  

M. K. Andersen    K. Raulund  -Rasmussen  H. C.B. Hansen  & B. W. Strobel 《European Journal of Soil Science》2002,53(3):491-502

Afforestation of sandy arable soils in northern Europe is likely to lead to an increase in the soil's acidity and changes in the behaviour of the organic matter, and this might affect the ability of the soil to retain heavy metals. It is important to assess the impact of such a change in the land use on the solubility of the heavy metals and to assess the risk of leaching to surface‐ and groundwater and the possible entrapment of heavy metals in the tree canopy. The impact of afforestation was assessed by excavating soil profiles in adjacent 34‐year‐old Norway spruce stands and arable plots at four different sites. We found that after 34 years the pH had decreased and cations were depleted in the topsoil under forest. The aqua regia‐extractable heavy metals were determined, and the heavy metal binding within the soil was assessed using a modified version of the BCR (Community Bureau of Reference) sequential extraction procedure. Higher contents of heavy metal were found in the arable plots in the loamy sand soils. Cadmium was found only in the most mobile fractions. The content of Pb in the subsoil was strongly correlated with the clay content, but not in the topsoil, which suggested that Pb had been added to the topsoil. We found strong correlations between the clay content and the Cu, Ni and Zn in the residual fraction, leading us to conclude that much of the Cu, Ni and Zn is of geological origin. No significant differences in the heavy metal fractionation between forest and arable soil were found, presumably because 34 years of different land use is not long enough to produce such differences.  相似文献   

Fluorescence of muscle and connective tissue from cod and salmon   总被引：1，自引：0，他引：1  

Andersen CM  Wold JP 《Journal of agricultural and food chemistry》2003,51(2):470-476

Autofluorescence of salmon and cod muscle was measured and compared with autofluorescence of collagen type I and type V. Similarities between fluorescence of fish muscle and collagen were found in that the same peaks were obtained around 390, 430, and 480 nm. These similarities are supported by principal component analyses. Texture and gaping score were predicted from the fluorescence spectra by partial least-squares regression. However, the predictions did not perform well. Relating fluorescence to the gaping score gave a prediction error of 0.91 and a correlation of 0.43 when measuring gaping on a scale from 0 to 5. There was no relation between texture and fluorescence spectra. Fluorescence of fish muscle could be related to the storage time. However, this relation seemed not to be induced by changes in collagen.  相似文献   

Elucidation of membrane destabilization in post-mortem muscles using an extracellular paramagnetic agent (Gd-DTPA): an NMR study     

Bertram HC  Stagsted J  Young JF  Andersen HJ 《Journal of agricultural and food chemistry》2004,52(20):6320-6325

The effect of Gd-DTPA on the development in NMR relaxation of skeletal rabbit muscles post-mortem was investigated by dynamic low-field (0.47 T) relaxation measurements from 4 min post-mortem and until 23 h post-mortem. Twelve rabbits were included in the study, and half of the animals were administered 0.2 mmol of Gd-DTPA iv 15 min before sacrifice, while the other half was administered an isotonic salt solution. A significant effect of Gd-DTPA treatment corresponding to a 25% reduction in the T(1) relaxation time was observed. T(2) relaxation was decomposed into two components reflecting intra- and extracellular components (T(2)()alpha and T(2)()beta, respectively), and Gd-DTPA treatment was found to affect both components. However, around 150 min post-mortem a dramatic increase in the difference between control and Gd-DTPA-treated rabbits was observed in the relaxation time of the intracellular water population (T(2)()alpha). Electrical stimulation of the muscles resulted in a significantly earlier onset of the increased effect of Gd-DTPA on the T(2)()alpha population. The increased effect of Gd-DTPA treatment on the T(2)()alpha component is believed to reflect leakage of water from the muscle cells due to membrane destabilization, known to be promoted by electrical stimulation. Accordingly, the present study demonstrates how Gd-DTPA can be used for probing membrane integrity in post-mortem muscles known to be of importance for subsequent water distribution and final water-holding capacity.  相似文献   

Lysosomal Fragility in Earthworms (Eisenia Veneta) Exposed to Heavy Metal Contaminated Soils from Two Abandoned Pyrite Ore Mines in Southern Norway     

Hønsi  Torunn G.  Stubberud  Hege E.  Andersen  Sjur  Stenersen  Jørgen 《Water, air, and soil pollution》2003,142(1-4):27-37

In this study we have investigated the toxicity of soils from thetwo abandoned pyrite mines Ertelien and Konnerud in the southernpart of Norway. Soil samples were collected close to the pitheadof the mines, and earthworms were exposed to different concentrations of the contaminated mine soil mixed with commercial plant soil for a period of 14 days. Life-cycle responses such as growth and survival, as well as the biomarkerresponse lysosomal fragility were measured. The body burdens ofthe four heavy metals Cu, Cd, Zn and Pb were detected in the worms, and the body concentration of the metals generally increased with increasing heavy metal concentration in the soil.The growth and mortality of the earthworms were not influenced when exposed to any of the mine soil concentrations used. The lysosomal fragility of the earthworm coelomocytes measured by the neutral red retention time (NRR-time) was found to be markedly reduced in all the exposed earthworms. This assay seemsto be a sensitive and dose dependent endpoint for the toxicity assessment of heavy metal mixtures in pyrite mine soils.  相似文献   

Decrease in Acid Deposition - Recovery in Norwegian Waters     

Brit Lisa Skjelkvåle  Kjetil Tørseth  Wenche Aas  Tom Andersen 《Water, air, and soil pollution》2001,130(1-4):1433-1438

Concentrations of sulphate in precipitation in southern Norway have decreased by 50–60% from 1980–1999. This has caused a decrease in sulphate concentrations in lakes of 30–40% from 1986–1999. Nitrogen in precipitation has decreased slightly over the last 10-years. In lakewater there has also been a significant but slight decrease. Concentrations of non-marine base cations in precipitation have decreased by 40% from 1980–1999. In lakewater, non-marine base cation concentrations have been at about the same level the last 10 years. This indicates that acid deposition has decreased sufficiently such that the pool of exchangeable base cations in the soil is now being replenished. The acidification situation in lakes in Norway has thus shown a clear improvement over the last 8–10 years. pH, alkalinity and ANC (acid neutralising capacity) have all increased. Concentrations of inorganic (toxic) aluminium species have decreased. The trends in H⁺ and Alⁿ⁺ do not follow the relation expected if Alⁿ⁺ concentrations were governed solely by a single solid phase of Al(OH)₃.  相似文献