Infectivity and Effectivity of Some Frankia Strains from the Rhamnaceae Family     

Margarita Caru  Angel Cabello 《Arid Land Research and Management》2013,27(1):53-59

The nodulation ability was determined of native Frankia strains (ChI1, ReI4, ReI6, TtI42, and TqI15) isolated from rhamnaceous species indigenous to Chile. All strains tested nodulated other Rhamnaceae host species within one or more genera, i.e., TtI42 nodulated seedlings of Trevoa trinervis, Retanilla ephedra, and Talguenea quinquenervia. Trees from other families such as Casuarina (Casuarinaceae) were not nodulated by the Frankia strains mentioned above. These results suggest that the five strains isolated from rhamnales could belong to the same cross inoculation group because they exhibited the same level of promiscuity. The effectivity of Frankia for nitrogen fixation was established by measurement of acetylene reduction activity in planta. Two strains (ReI6 and TtI42) were found more effective than the others and improved the total dry weight and shoot height of Retanilla ephedra seedlings. These studies may facilitate the choice of selected rhamnaceous Frankia pairs for arid soil afforestation, since these plants are naturally adapted to this environment in Chile.  相似文献   

Systemic hypertension in dogs with leishmaniasis: prevalence and clinical consequences     

Cortadellas O  del Palacio MJ  Bayón A  Albert A  Talavera J 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2006,20(4):941-947

A prospective study was performed (November 1998 to December 2003) to determine the prevalence of systemic hypertension (SH) in dogs with glomerular disease secondary to leishmaniasis. One hundred and five dogs with leishmaniasis were screened and staged for the presence of renal disease (RD) and SH. For the purpose of the study, RD was defined as serum creatinine concentration > or = 1.4 mg/dL, a urine protein/creatinine ratio > or = 0.5, or both. SH was defined as a systolic blood pressure (SBP) > or =180 mm Hg or an SBP between 150 and 179 mm Hg in the presence of clinical manifestations of SH. Fifty-two (49.5%) of the dogs had some degree of RD, and 32 (61.5%) of these dogs were diagnosed with SH. Moreover, SH also was diagnosed in 3 dogs without RD. Left ventricular hypertrophy (LVH), estimated by echocardiography, was the most frequently observed systemic consequence of hypertension, being present in 32 (91.4%) of the hypertensive dogs. Echocardiographic abnormalities were not detected in any of the 33 dogs with leishmaniasis without RD, which were used as controls. Ocular consequences of SH were observed in only 2 (5.7%) of the dogs with hypertension. We conclude that SH is prevalent in dogs with RD secondary to leishmaniasis, not only in the more severe stages but also in the early course of the illness before azotemia becomes apparent. Canine leishmaniasis may be a useful natural model to study SH secondary to glomerular disease.  相似文献   

COX-2 and sPLA2 inhibitory activity of aqueous extract and polyphenols of Rhizophora mangle (red mangrove)     

Marrero E  Sánchez J  de Armas E  Escobar A  Melchor G  Abad MJ  Bermejo P  Villar AM  Megías J  Alcaraz MJ 《Fitoterapia》2006,77(4):313-315

The aqueous extract of Rhizophora mangle bark and its polyphenolic fractions showed remarkable in vitro antiinflammatory activity in a preliminary study. The low molecular weight fraction exhibited cyclooxygenase-2 inhibitory activity while the total aqueous extract and the low molecular weight fraction showed secretory phospholipase A(2) inhibitory activity.  相似文献   

Sensitivity of CERES-Maize simulated yields to uncertainty in soil properties and daily solar radiation     

Federico E. Bert  Carlos E. Laciana  Guillermo P. Podestá  Emilio H. Satorre  Angel N. Menéndez 《Agricultural Systems》2007

The sensitivity of CERES-Maize yield predictions to uncertainty in a set of soil-related parameters and solar radiation was evaluated in Pergamino, in the Argentine Pampas. Maize yields were simulated for Pergamino using a 31 years climatic record for a range of values of a group of important model input variables. The input variables considered (and the range evaluated) were: soil nitrogen content at sowing (from 20 to 80 Kg ha⁻¹), soil organic matter content (from 1.75% to 4%), soil water storage capacity (from 150 to 200 mm), soil water content at sowing (from 50% to 100% of total available water), soil infiltration curve number (from 76 to 82) and daily solar radiation (from −20% to 12% of the historical values). Then, a sensitivity analysis using a combination of mathematical and graphical approaches was performed to evaluate the model response to changes in the values of the input variables considered. Moreover, a simplified method based on the evaluation of the model sensitivity at extreme values of the input variables is proposed to evaluate the model non-linear responses with a reduced number of runs. Under the scenario evaluated, representative of the typical maize productions systems of the Argentine Pampas, the model results showed higher sensitivity to changes in radiation (normalized sensitivity were −0.69 and 0.45 for rainfed and irrigated conditions, respectively) than for the soil variables (normalized sensitivity ranged from 0.20 to 0.28). The CERES-Maize model was found to have similar sensitivity for the different soil inputs. Furthermore, some of the variables evaluated (soil curve number, soil water content at sowing and radiation under rainfed conditions) showed an important non-linear response.  相似文献   

Metal concentrations in agricultural and forestry soils in northwest Spain: implications for disposal of organic wastes on acid soils   总被引：2，自引：0，他引：2  

M.E. López-Mosquera  R. Barros  M.J. Sainz  E. Carral  S. Seoane 《Soil Use and Management》2005,21(3):298-305

Abstract In Galicia (northwest Spain) the application of organic wastes to agricultural land is a common practice, which may increase total and bioavailable metal contents in the soil. In this study, total metal concentrations were determined in acid soils under different use (pasture, cropland, woodland) in an agricultural area where agro-industrial sludges are frequently recycled as manure. The aim was to establish baseline metal levels which could be used to determine the capacity of soils to absorb organic wastes. The estimation of baseline metal concentrations was carried out by two methods, one based on the analysis of means and geometric deviations, and another based on a modal analysis. Results suggested that the modal analysis procedure might be preferable when analysing data sets with a heterogeneous frequency distribution. In general, there was no significant difference in total metal concentrations when comparing soils from different land uses. Baseline levels for each metal indicated that all soils were suitable for organic waste application under current European Union (EU) legislation. From 2015, more restrictive metal limit values have been proposed by the EU, potentially preventing the addition of metal-containing wastes to pasture, cropland and woodland soils. The dissolved metal values in each soil were also estimated by empirical equations relating total metal concentrations, pH and organic matter content. Results showed that only the pasture soils would be suitable for organic waste disposal under the proposed EU metal limits for 2015, due to liming and substantial organic matter content. Total metal concentrations were insufficient to discriminate environmental risk in acid soils of different land use. The determination of baseline levels in reference areas and the estimation of soil metal bioavailability are suggested to define permissible values in the developing legislation.  相似文献   

Mineral balance in milk heated using microwave energy     

de la Fuente MA  Olano A  Juárez M 《Journal of agricultural and food chemistry》2002,50(8):2274-2277

Milk heated to 75 and 85 degrees C in a water bath or in a microwave oven was assayed for changes in salt partitioning after cooling to room temperature. To properly to assess differences and draw valid comparisons, the two heating methods used in the experiment were applied to samples for identical exposure times, and the samples were heated to attain the same final temperatures. Although the soluble Ca and P(i) contents were lower in the heated milk samples, no significant differences in salt partitioning were found between microwave and conventional heating. Ionic calcium levels in the milk samples pasteurized using microwave energy were very close to the levels in the samples heated in a conventional water bath (approximately 90% of the level in the untreated milk samples). The microwave heating-induced changes were completely reversed after storage at 20 degrees C for 24 h. The coagulation properties of the heated milk samples were also examined, and the coagulation time was longer and the curd formation rate slower in the microwave-heated milk than in the raw milk. Still, the experimental results demonstrated that microwave heating was no more detrimental to the milk than conventional heating and could thus be used for pasteurization purposes.  相似文献   

Content of free phenolic compounds in cultivars of potatoes harvested in Tenerife (Canary Islands)     

Verde Méndez Cdel M  Rodríguez Delgado MA  Rodríguez Rodríguez EM  Díaz Romero C 《Journal of agricultural and food chemistry》2004,52(5):1323-1327

Determination of free phenolic compounds in potato samples was optimized using a high-performance liquid chromatographic (HPLC) method with on-line diode array detection. This method was applied to samples of four cultivars of potatoes harvested in Tenerife (Canary Islands). The free phenolic compounds found in the potato samples were (+)-catechin, chlorogenic acid, caffeic acid, p-coumaric acid, and ferulic acid. Potato samples belonging to Colorada cultivar, ssp. andigena, had mean concentrations of total phenolic compounds and chlorogenic acid higher than those found for Kerr's Pink and Cara cultivars, ssp. tuberosum, and for Negra cultivar, S. x chaucha. In contrast, p-coumaric acid was not detected in any potato samples of the Colorada cultivar. Traditional potatoes presented a higher mean concentration of ferulic acid than recently imported potatoes. A significant and negative correlation was established between (+)-catechin and p-coumaric acid. A considerable contribution to the daily intake of flavonoids was observed with the actual consumption of potatoes.  相似文献   

Effect of processing techniques at industrial scale on orange juice antioxidant and beneficial health compounds   总被引：15，自引：0，他引：15  

Gil-Izquierdo A  Gil MI  Ferreres F 《Journal of agricultural and food chemistry》2002,50(18):5107-5114

Phenolic compounds, vitamin C (L-ascorbic acid and L-dehydroascorbic acid), and antioxidant capacity were evaluated in orange juices manufactured by different techniques. Five processes at industrial scale (squeezing, mild pasteurization, standard pasteurization, concentration, and freezing) used in commercial orange juice manufacturing were studied. In addition, domestic squeezing (a hand processing technique) was compared with commercial squeezing (an industrial FMC single-strength extraction) to evaluate their influences on health components of orange juice. Whole orange juice was divided into soluble and cloud fractions after centrifugation. Total and individual phenolics were analyzed in both fractions by HPLC. Commercial squeezing extracted 22% more phenolics than hand squeezing. The freezing process caused a dramatic decrease in phenolics, whereas the concentration process caused a mild precipitation of these compounds to the juice cloud. In pulp, pasteurization led to degradation of several phenolic compounds, that is, caffeic acid derivatives, vicenin 2 (apigenin 6,8-di-C-glucoside), and narirutin (5,7,4'-trihydroxyflavanone-7-rutinoside) with losses of 34.5, 30.7, and 28%, respectively. Regarding vitamin C, orange juice produced by commercial squeezing contained 25% more of this compound than domestic squeezing. Mild and standard pasteurization slightly increased the total vitamin C content as the contribution from the orange solids parts, whereas concentration and freezing did not show significant changes. The content of L-ascorbic acid provided 77-96% of the total antioxidant capacity of orange juice. Mild pasteurization, standard pasteurization, concentration, and freezing did not affect the total antioxidant capacity of juice, but they did, however, in pulp, where it was reduced by 47%.  相似文献   

Validation of the Spanish version of the physical activity questionnaire used in the Nurses' Health Study and the Health Professionals' Follow-up Study     

Martínez-González MA  López-Fontana C  Varo JJ  Sánchez-Villegas A  Martinez JA 《Public health nutrition》2005,8(7):920-927

OBJECTIVE: The objective of this analysis was to test the validity of the estimates of energy expenditure and sedentary lifestyle obtained through a self-administered questionnaire of physical activity for Spanish-speaking people adapted from US questionnaires (Nurses' Health Study and Health Professionals' Follow-up Study) using a triaxial accelerometer (RT3 Triaxial Research Tracker) as the reference. DESIGN AND SETTING: Validation study, calculating the non-parametric correlation coefficients between the level of physical activity and sedentary lifestyle collected by the self-administered questionnaire and the triaxial accelerometer measurements. Percentage of misclassification and kappa coefficients were also calculated. SUBJECTS: The study population consisted of a sample of 40 obese women who were participants of the SUN (Seguimiento Universidad de Navarra) project (a prospective cohort study among Spanish university alumni). They were selected because of their peculiar metabolic characteristics, in the search for a sub-optimal scenario for validity. RESULTS: Physical activity during leisure time (estimated as MET-h week(-1)) derived from the self-administered questionnaire moderately correlated with kcal day(-1) assessed through the accelerometer (Spearman's rho = 0.507, 95% confidence interval (CI) 0.232, 0.707). The Spearman correlation between the ratio of sedentary lifestyle to physical activity obtained through the questionnaire and the direct estimation (RT3) was -0.578 (95% CI -0.754, -0.325). The kappa index was 0.25 (P = 0.002) when assessing the cross-classification into quintiles and 0.41 for the dichotomous estimation of a sedentary lifestyle. Only 2.5% of participants were misclassified by the questionnaire more than two quintiles apart from the estimates of the RT3. CONCLUSIONS: The moderate values obtained for correlation in a sub-optimal scenario for validity and the low percentage of extreme misclassification suggest the validity of the questionnaire to assess physical activity in Spanish-speaking women aged 20-50 years.  相似文献   

Identification of casein phosphopeptides released after simulated digestion of milk-based infant formulas     

Miquel E  Gómez JA  Alegría A  Barberá R  Farré R  Recio I 《Journal of agricultural and food chemistry》2005,53(9):3426-3433

Adapted, follow-up, probiotic follow-up, toddler, and probiotic toddler infant formulas were subjected to an in vitro enzymatic procedure simulating physiological digestion. The formation and identification of casein phosphopeptides (CPPs) in the milk-based infant formulas were studied using reversed phase high-performance liquid chromatography coupled on line to an ion trap mass spectrometer. Most CPPs formed contained the cluster sequence SpSpSpEE, a mineral binding site. Phosphopeptide alpha(s2)-CN(1-19)4P was present in all formulas analyzed. Probiotic formulas released CPPs not detected in nonprobiotic formulas and probably formed by bifidobacteria action. These observations suggest that physiological digestion of these products promotes the formation of bioactive peptides with mineral carrier properties in the gastrointestinal tract, which resist further proteolysis.  相似文献