Enhanced skeletal growth of sheep heterozygous for an inactivated fibroblast growth factor receptor 3     

Smith LB  Dally MR  Sainz RD  Rodrigue KL  Oberbauer AM 《Journal of animal science》2006,84(11):2942-2949

Normal fibroblast growth factor receptor 3 (FGFR3) acts as a negative bone growth regulator by restricting chondrocyte proliferation and endochondral bone elongation. In sheep, a heritable mutation that inactivates FGFR3 produces skeletal overgrowth when homozygous, this condition is commonly referred to as spider lamb syndrome (SLS). We hypothesized that sheep heterozygous for the inactivated FGFR3 mutation (FGFR3(SLS/+)) would exhibit enhanced long bone growth and greater frame size; additionally, the isolated effects of increased bone growth would translate into greater BW and larger LM area relative to normal lambs at harvest. The current study investigated bone length and LM area of FGFR3(SLS/+) sheep at maturity and during growth. At maturity, FGFR3(SLS/+) ewes exhibited a larger frame size and longer bones than normal FGFR3(+/+) ewes (P < 0.05). Similarly, FGFR3(SLS/+) lambs had greater frame sizes than normal FGFR3(+/+) lambs, as indicated by increased metacarpal III length and height at withers (P < 0.05). The FGFR3(SLS/+) lambs took longer than the normal FGFR3(+/+) lambs to reach the 60-kg common BW harvest end point (P < 0.05). The FGFR3(SLS/+) lambs showed no difference in BW, ADG, or LM area at any age compared with normal FGFR3(+/+) lambs (P > 0.2). A similar LM area produced in the context of a greater frame size and skeletal length produces a greater muscle volume, thereby potentially increasing meat yield. The results of this study suggest that FGFR3(SLS/+) animals exhibit a relaxation of the normal inhibition of chondrocyte proliferation, resulting in an increase in the overall frame size. The sheep industry could utilize the naturally occurring genetic mutation in FGFR3 to potentially increase meat yields with enhanced skeletal growth as an alternative to exogenous growth promotants.  相似文献   

Effects of age on body condition and production parameters of multiparous beef cows     

Renquist BJ  Oltjen JW  Sainz RD  Calvert CC 《Journal of animal science》2006,84(7):1890-1895

Pregnancy rate, calving interval, birth weight, weaning weight, and quarterly BCS were collected for 5 consecutive years on 454 fall-calving multiparous British crossbred cattle (3 to 10 yr of age) to evaluate associations of age with BCS and production parameters. Body weight and BCS were collected pre-calving, prebreeding, at weaning, and midway through the second trimester of pregnancy (August). Body condition score was correlated with age during all seasons (P < 0.01). At calving, breeding, and in August, 3-yr-old cows had the lowest BW and BCS, whereas 8-yr-old cows had the greatest. At weaning, these values were maximal in 10-yr-old cows. Pregnancy rate was near 80% up to 9 yr of age but decreased to 57% in 10-yr-old cows. The relationship of pregnancy rate with age appears to be correlated with the BCS decrease at breeding in the older cows, supported by the fact that inclusion of BCS at breeding in the statistical model eliminated the effect of age on pregnancy rate (P = 0.42). Calving interval was longer in 3-yr-old cows compared with 4- to 9-yr-old cows (P = 0.02); however, among older cows, there was little change in the calving interval. Birth weight reached a maximum at 8 yr of age (35 +/- 0.9 kg) and a minimum in 3-yr-old cows (32 +/- 0.7 kg). Birth weights of calves born to both 3- and 4-yr-old cows were lower than for those born to 5-, 6-, 7-, or 8-yr-old cows (P < 0.05). Ten-year-old cows weaned lighter calves (205-d adjusted weaning weight) than younger dams. Furthermore, 3-yr-old cows weaned calves 9 +/- 2.1 and 14 +/- 2.4 kg lighter than 4- and 5-yr-old cows, respectively (P < 0.001). Interpretation of the age analyses of calving interval, birth weight, and weaning weight was independent of the inclusion of BCS in the model. This study documents the effects of age on calving interval, birth weight, and weaning weight that are independent of BCS.  相似文献   

Total and water-soluble phosphorus excretion from swine fed low-phytate soybeans   总被引：1，自引：0，他引：1  

Powers WJ  Fritz ER  Fehr W  Angel R 《Journal of animal science》2006,84(7):1907-1915

A study was conducted to evaluate the impact of feeding soybean meal (SBM) from low-phytate (LP) or traditional phytate (TP) soybeans on performance and excretions from growing swine. Ninety-six crossbred barrows (initial BW = 18 +/- 0.3 kg) were allocated by BW to 24 pens and fed 1 of 4 treatment diets: TP SBM without supplemental phytase; TP SBM plus 500 phytase units of phytase/kg, as-fed basis [Ronozyme P (CT) 2500; DSM Nutritional Products, Basel, Switzerland]; LP SBM (USDA-ARS breeding line CX1834-1) without supplemental phytase, and LP SBM plus phytase. All diets within a feeding phase were formulated to be isocaloric and have similar available Lys and nonphytin P content. Pens were assigned randomly to treatments at the beginning of each of the 4 feeding phases. An indigestible marker was added to the mash feed. Individual pig weights and fecal samples were collected, and feed disappearance by pen was recorded weekly. No phytase inclusion or SBM source effects were observed for pen ADG, ADFI, or G:F (P > 0.05). Total tract apparent digestibility of DM and OM was not different among treatment groups (P > 0.05). Apparent digestibility of P was greater for pigs fed diets containing the LP SBM (48.9 vs. 42.4%; P < 0.01) and less when diets included phytase (44.3 vs. 47.0%; P < 0.0001). Total P (tP) and water-soluble P (WSP) excreted were affected by dietary treatment (tP: 20.0, 18.0, 16.8, and 13.8 g/kg of feces DM, P < 0.01; and WSP: 10.9, 10.1, 9.1, and 8.5 g/kg, P < 0.01, for TP SBM without supplemental phytase, TP SBM plus 500 phytase units of phytase/kg, LP SBM without supplemental phytase, and LP SBM plus phytase diets, respectively). Inclusion of phytase decreased tP and WSP excreted (P < 0.01), as did use of LP SBM (P < 0.01). Diet effects on the fraction of excreted tP that was WSP were observed (P < 0.01); however, there was not a significant effect of SBM source. Inclusion of exogenous phytase in diets increased the proportion of tP that was excreted as WSP from 55% in diets without phytase to 59% in diets containing phytase. The findings suggest that there is a need for LP soybeans as a dietary component to minimize environmental impacts.  相似文献   

Gradients of abundance of fish across no‐take marine reserve boundaries: evidence from Philippine coral reefs     

Rene A. Abesamis  Garry R. Russ  Angel C. Alcala 《水产资源保护:海洋与淡水生态系统》2006,16(4):349-371

• 1. An abundance gradient from high inside to low outside a no‐take marine reserve may indicate net emigration of adult fish from the reserve (‘spillover’).
• 2. We examined spatial patterns of abundance of fish across two ～900 m long sections of coral reef slope at each of two small Philippine islands (Apo and Balicasag). One section sampled the entire length of a no‐take reserve and extended 200–400 m outside the two lateral reserve boundaries. The other section, without a reserve, was a control. The reserves had had 20 (Apo) and 15 (Balicasag) years of protection when sampled in 2002.
• 3. Significant spatial gradients of decreasing abundance of target fish occurred across only one (Apo Reserve northern boundary = ARNB) of four real reserve boundaries, and across none of the control ‘boundaries’. Abundance of non‐target fish did not decline significantly across reserve boundaries.
• 4. Abundance of target fish declined sharply 50 m outside the ARNB, but enhanced abundance extended 100–350 m beyond this boundary, depending on fish mobility.
• 5. Density of sedentary target fish declined 2–6 times faster than density of highly vagile and vagile target fish across the ARNB.
• 6. Habitat factors could not account for these ARNB results for target fish, but did influence abundance patterns of non‐target fish.
• 7. The lack of abundance gradients of target fish at Balicasag may reflect reduced fishing outside the reserve since it was established.
• 8. Apo Reserve had a gradient of abundance of target fish across at least one boundary, a result consistent with spillover.

Copyright © 2006 John Wiley & Sons, Ltd.  相似文献   

Monounsaturated fatty acids, olive oil and blood pressure: epidemiological, clinical and experimental evidence     

Alonso A  Ruiz-Gutierrez V  Martínez-González MA 《Public health nutrition》2006,9(2):251-257

Diet has an important role in the prevention and treatment of hypertension. In early epidemiological studies, conducted mainly in the USA, monounsaturated fatty acids showed a deleterious association with blood pressure or no relationship at all. However, more recent studies, conducted in Mediterranean countries, have shed new light on this issue. In the present review we summarise the main results of epidemiological studies and feeding trials, and explain the possible mechanisms through which monounsaturated fatty acids, and specifically olive oil as the major dietary source of this type of fat in Mediterranean countries, could exert a favourable effect on blood pressure.  相似文献   

Molecular analyses of evolution and population structure in a worldwide almond [Prunus dulcis (Mill.) D.A. Webb syn. P. amygdalus Batsch] pool assessed by microsatellite markers     

Angel Fernández i Martí  Carolina Font i Forcada  Kazem Kamali  María J. Rubio-Cabetas  Michelle Wirthensohn  Rafel Socias i Company 《Genetic Resources and Crop Evolution》2015,62(2):205-219

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Application of relative quantification TaqMan real-time polymerase chain reaction technology for the identification and quantification of Thunnus alalunga and Thunnus albacares     

Lopez I  Pardo MA 《Journal of agricultural and food chemistry》2005,53(11):4554-4560

A novel one-step methodology based on real-time Polymerase Chain Reaction (PCR) technology has been developed for the identification of two of the most valuable tuna species. Nowadays, species identification of seafood products has a major concern due to the importing to Europe of new species from other countries. To achieve this aim, two specific TaqMan systems were devised to identify Thunnus alalunga and Thunnus albacares. Another system specific to Scombroidei species was devised as a consensus system. In addition, a relative quantification methodology was carried out to quantify T. alalunga and T. albacares in mixtures after the relative amount of the target was compared with the consensus. This relative quantification methodology does not require a known amount of standard, allowing the analysis of many more samples together and saving costs and time. The utilization of real-time PCR does not require sample handling, preventing contamination and resulting in much faster and higher throughput results.  相似文献   

Identification of a genomic clone to ACC oxidase from papaya (Carica papaya L.) and expression studies     

López-Gómez R  Morales-Domínguez F  Mendoza Alcázar O  Gómez-Lim MA 《Journal of agricultural and food chemistry》2004,52(4):794-800

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Generation of furosine and color in infant/enteral formula-resembling systems     

Rufián-Henares JA  García-Villanova B  Guerra-Hernández E 《Journal of agricultural and food chemistry》2004,52(17):5354-5358

The extent of the Maillard reaction was studied by measuring furosine and color formation in infant and enteral formula-resembling model systems prepared by mixing calcium caseinate, laboratory-obtained or commercial whey protein with lactose or dextrinomaltose (ingredients similar to those used in infant and enteral formula manufacture) and heating the mixture at 100, 120, or 140 degrees C for 0-30 min. The furosine determination was performed by HPLC and the color determination by measuring colorimetric parameters L, a, and b in a reflection photometer. The first steps of the Maillard reaction could be followed by furosine determination when initial ingredients had low thermal damage. Hence, furosine may be an indicator of low thermal damage in ingredients with <100 mg/100 g of protein. At the concentrations used in these model systems, similar to those in infant and enteral formulas, furosine values (indirect measure of lysine losses) were higher in lactose than in dextrinomaltose systems, in which only glucose, maltose, maltotriose, and maltotetraose among all of the sugars present showed reactivity with casein. Finally, the advanced steps could be followed by color determination when the initial ingredients had high thermal damage or the model systems were heated at high temperature or for a long time. Among the parameters assayed, b was the most sensitive.  相似文献   

Soil penetrometer resistance spatial variability in a Ferralsol at several soil moisture conditions     

Angel Utset  Greco Cid 《Soil & Tillage Research》2001,61(3-4):193-202

The penetrometer resistance (PR) spatial variability in a Ferralsol under different soil moisture conditions was characterized through statistical and geostatistical methods. PR measurements were made in a 10-row, 10-columns, 3-m spacing grid at 20–30 cm depth using a hand-pushing penetrometer. Measurements were made for dry soil conditions (before irrigation) as well as 2 and 24 h after irrigation. The soil bulk density (BD) and the relative topographical altitudes (RT) were measured at the same locations. The PR spatial variability is normally distributed for dry soil conditions and after irrigation-water redistribution. A normal distribution fits also to the BD spatial variability. PR mean and coefficients of variations are lower for wet soil conditions and vary notably according to the soil moisture condition. The PR semivariance values are much higher for dry soil conditions than those found for wet soil conditions. However, the semivariogram of PR before irrigation shows almost a pure nugget effect. Irrigation yields a spatial structure in PR measurements. Despite the differences in the shown spatial structure, the range of the PR semivariograms before and 24 h after irrigation is about 8–10 m, which is very similar to the range of the BD semivariogram. The correlation coefficients between PR and the other measured variables are very small before and after irrigation, which could lead to the conclusion that those variables are not related at all. However, according to the co-dispersion coefficients plotted as a function of the separation lag, the correlation between those variables changes according to the separation distance. Particularly, PR and the RT show correlation 24 h after irrigation only for lags between 5 and 12 m. The results pointed out that PR spatial variability depends on soil moisture condition, soil BD and on several local features as microtopography.  相似文献