Histopathological Changes in Third-Instar and Adult Anastrepha ludens (Diptera: Tephritidae) After in vitro Heat Treatment     

Lorena Caro-Corrales  Jose Caro-Corrales  Angel Valdez-Ortiz  Jose Lopez-Valenzuela  Hector Lopez-Moreno  Daniel Coronado-Velazquez  Emilio Hernandez-Ortiz  Jose Rendon-Maldonado 《Journal of insect science (Online)》2015,15(1)

The Mexican fruit fly, Anastrepha ludens Loew (Diptera: Tephritidae), is one of the most harmful pests of mango causing direct damage by oviposition on the fruit pulp. Mango for export is subjected to hydrothermal treatment as a quarantine method for the control of this pest, but exposure to heat for long periods of time reduces considerably the quality and shelf-life of treated fruit. The aim of this work was to study morphological changes of third-instar larvae and adults of A. ludens after in vitro exposure to high temperature at sublethal times. A heating block system was used to expose larvae at 46.1°C for 19.6 and 12.9 min, producing 94.6 and 70% mortality, respectively. Treated larvae were processed for optical microscopy. A fraction of surviving treated larvae was separated into containers with artificial diet to allow development into adults. Adult sexual organs were dissected and processed for transmission electron microscopy analysis. Results showed that 94.6% of the treated larvae died at 46.1°C for 19.6 min and none of the surviving larvae eclosed to adulthood, as they developed as malformed puparia. For the in vitro treatment at 46.1°C during 12.9 min, 70% of the treated larvae died and only 3.75% reached the adult stage, but ultrastructural damage in the male testes and in the female ovaries was observed. Additionally, 11.1% of the adult flies from the in vitro treatment also showed wing malformation and were incapable of flying. The analysis showed that surviving flies were unable to reproduce.  相似文献   

Determination of organic acids in tissues and exudates of maize, lupin, and chickpea by high-performance liquid chromatography-tandem mass spectrometry     

Erro J  Zamarreño AM  Yvin JC  Garcia-Mina JM 《Journal of agricultural and food chemistry》2009,57(10):4004-4010

This article describes a fast and simple methodology for the extraction and determination of organic acids in tissues and root exudates of maize, lupin, and chickpea by LC/MS/MS. Its main advantage is that it does not require sample prepurification before HPLC analysis or sample derivatization to improve sensibility. The results obtained showed good precision and accuracy, a recovery close to 100%, and no significant matrix effect. Moreover, the sensibility of the method is in general better than that of previously described methodologies, with detection limits between 15 and 900 pg injected.  相似文献   

Fine-Scale Spatial Genetic Structure in Perennial Grasses in Three Environments     

Steven E. Smith  Tulio Arredondo  Martín Aguiar  Elisabeth Huber-Sannwald  Angel Alpuche  Armando Aguado  Oscar A. Grageda  Kandres Halbrook  Cecilia Bottini 《Strength and Conditioning Journal》2009,62(4):356-363

Past research has shown that changes in grazing-resistance traits may be associated with genetic changes in plant populations. Little is known about spatial genetic relationships within plant populations (spatial genetic structure) and any grazing effects on these relationships. Here we present observations of the fine-scale spatial genetic structure in three grass species in semiarid environments (Arizona, Mexico, and Argentina). In each environment, populations of a dominant grass species were sampled from two sites with contrasting livestock grazing histories. Plant genotypes were described with the use of amplified fragment length polymorphism markers. In Arizona, populations of sideoats grama (Bouteloua curtipendula var. caespitosa Gould and Kapadia) differed in that one has never experienced livestock grazing, whereas cattle have grazed the other. In the other two environments, populations exposed to long-term heavy grazing were examined, along with those that experienced either moderate grazing (Mexico, blue grama [Bouteloua gracilis {Willd. ex Kunt} Lag. ex Griffiths]) or extended exclusion of livestock (Argentina [Poa ligularis Nees ex Steud.]). Based on independent analysis of each population, we observed no differences in average gene diversity between populations of each species. With the use of analysis of molecular variance we found slight but significant genetic differentiation between populations with different grazing histories in Arizona and Argentina. Significant genetic structure was present in all populations and indicated an inverse relationship between spatial and genetic distance. Interestingly, this relationship was most pronounced in the cattle-free sideoats grama population, suggesting larger genetic neighborhood areas in the absence of livestock. Less distinct differences in spatial genetic structure associated with grazing history were evident in the other two species. We hypothesize that livestock grazing may lead to increased homogeneity in genetic structure at the landscape scale. Effectively examining this hypothesis presents many experimental challenges.  相似文献   

Results from an indirect fluorescent antibody test using three different strains of Ehrlichia canis     

Aguirre E  Ayllón T  Sainz A  Amusategui I  Villaescusa A  Rodríguez-Franco F  Tesouro MA 《Veterinary journal (London, England : 1997)》2009,182(2):301-305

An indirect fluorescent antibody (IFA) test is usually performed to detect antibodies in dogs naturally infected by Ehrlichia canis. In this work, results obtained using three different E. canis strains as antigen (a commercial antigen, the E. canis Oklahoma strain and the E. canis Madrid strain) were compared. One hundred and forty-nine serum samples obtained from dogs living in the centre of Spain were analysed. When qualitative results were evaluated, identical results were detected in 87.2% of samples for the three antigens tested. When comparing antibody titre results, differences between the Madrid strain and the commercial antigen, and between the Madrid and Oklahoma strains were statistically significant (P < 0.0001). No differences were found when comparing the Oklahoma strain with the commercial antigen (P = 0.562). Subtle intra-laboratory variations shown in this study suggest a higher sensitivity of the IFA test when an autochthonous strain is used as antigen.  相似文献   

Toxicity of cultured bullseye puffer fish Sphoeroides annulatus     

Nuñez-Vazquez EJ  Garcia-Ortega A  Campa-Cordova AI  Abdo de la Parra I  Ibarra-Martinez L  Heredia-Tapia A  Ochoa JL 《Marine drugs》2012,10(2):329-339

The toxin content in various life cycle stages of tank-cultivated bullseye puffer (Sphoeroides annulatus) were analyzed by mouse bioassay and ESI-MS spectrometry analysis. The presence of toxin content was determined in extracts of sperm, eggs, embryo, larvae, post-larvae, juvenile, pre-adult, and adult fish, as well as in food items used during the cultivation of the species. Our findings show that only the muscle of juveniles, the viscera of pre-adults, and muscle, liver, and gonad of adult specimens were slightly toxic (<1 mouse unit). Thus, cultivated S. annulatus, as occurs with other cultivated puffer fish species, does not represent a food safety risk to consumers. This is the first report of toxin analysis covering the complete life stages of a puffer fish under controlled conditions.  相似文献   

Phlorotannin Extracts from Fucales Characterized by HPLC-DAD-ESI-MSn: Approaches to Hyaluronidase Inhibitory Capacity and Antioxidant Properties     

Federico Ferreres  Graciliana Lopes  Angel Gil-Izquierdo  Paula B. Andrade  Carla Sousa  Teresa Mouga  Patrícia Valent?o 《Marine drugs》2012,10(12):2766-2781

Purified phlorotannin extracts from four brown seaweeds (Cystoseira nodicaulis (Withering) M. Roberts, Cystoseira tamariscifolia (Hudson) Papenfuss, Cystoseira usneoides (Linnaeus) M. Roberts and Fucus spiralis Linnaeus), were characterized by HPLC-DAD-ESI-MSⁿ. Fucophloroethol, fucodiphloroethol, fucotriphloroethol, 7-phloroeckol, phlorofucofuroeckol and bieckol/dieckol were identified. The antioxidant activity and the hyaluronidase (HAase) inhibitory capacity exhibited by the extracts were also assessed. A correlation between the extracts activity and their chemical composition was established. F. spiralis, the species presenting higher molecular weight phlorotannins, generally displayed the strongest lipid peroxidation inhibitory activity (IC₅₀ = 2.32 mg/mL dry weight) and the strongest HAase inhibitory capacity (IC₅₀ = 0.73 mg/mL dry weight). As for superoxide radical scavenging, C. nodicaulis was the most efficient species (IC₅₀ = 0.93 mg/mL dry weight), followed by F. spiralis (IC₅₀ = 1.30 mg/mL dry weight). These results show that purified phlorotannin extracts have potent capabilities for preventing and slowing down the skin aging process, which is mainly associated with free radical damage and with the reduction of hyaluronic acid concentration, characteristic of the process.  相似文献   

High Antioxidant Activity Mixture of Extruded Whole Quality Protein Maize and Common Bean Flours for Production of a Nutraceutical Beverage Elaborated with a Traditional Mexican Formulation     

Cuauhtémoc Reyes-Moreno  Oscar D. Argüelles-López  Jesús J. Rochín-Medina  Jorge Milán-Carrillo  Javier Rivera-López  Angel Valdez-Ortiz  José A. López-Valenzuela  Roberto Gutiérrez-Dorado 《Plant foods for human nutrition (Dordrecht, Netherlands)》2012,67(4):450-456

The objective of this study was to determine the best combination of extrusion process variables for the production of whole quality protein maize (EQPMF) and common bean (ECBF) flours to prepare a high antioxidant activity mixture (EQPMF + ECBF) suitable to produce a nutraceutical beverage with high acceptability elaborated with a traditional Mexican formulation. Processing conditions were obtained from a factorial combination of barrel temperature (BT?=?120–170 °C) and screw speed (SS?=?120–200 rpm). Response surface methodology was applied to obtain maximum values for antioxidant activity (A _ox A) of the flour mixture (EQPMF + ECBF) and acceptability (A) of the nutraceutical beverage. The best combinations of extrusion process variables for EQPMF and ECBF to prepare an optimized mixture (60%EQPMF?+?40%ECBF) were BT?=?98 °C/SS?=?218 rpm and BT?=?105 °C/SS?=?83 rpm, respectively. The optimized mixture had A _ox A?=?14,320 μmol Trolox equivalent (TE)/100 g sample dry weight (dw) and a calculated protein efficiency ratio (C-PER) of 2.17. A 200 ml portion of a beverage prepared with 25 g of the optimized flour mixture had A _ox A?=?3,222 μmol TE, and A?=?89 (level of satisfaction “I like it extremely”). This nutraceutical beverage could be used as an alternative to beverages with low nutritional/nutraceutical value, such as those prepared with water, simple sugars, artificial flavoring and colorants, which are widely offered in the market.  相似文献   

Improved Microbial and Chemical Reduction of Direct Blue 71 Using Anthraquinone-2,6-disulfonate Immobilized on Granular Activated Carbon     

Luis H. Alvarez  Yair A. Del Angel  Bernardo García-Reyes 《Water, air, and soil pollution》2017,228(1):38

The aim of this study was to evaluate the redox mediating capacity of anthraquinone-2,6-disulfonate (AQDS) immobilized on granular activated carbon (GAC) during the reductive decolorization of direct blue 71 (DB71) under microbial and chemical conditions. The immobilization of AQDS on GAC was conducted by adsorption, and it has obtained an uptake capacity of 0.227 mmol g^?1. The anchorage of AQDS on GAC improved its electron transfer capacity (ETC) up to 2.05 times higher than the raw material. Similarly, the addition of GAC-AQDS increased up to 1.75- and 1.16-fold the rate of decolorization (k _d ) of DB71 under microbial and chemical conditions, respectively, in comparison to the unmodified GAC. Surprisingly, a higher k _d value was achieved in incubations without either GAC or GAC-AQDS because of the generation of aromatic amines, from the reduction DB71, taking into account that these species may act as a catalyst in the DB71 reduction process. In contrast, adsorption of aromatic amines on either GAC or GAC-AQDS decreased its redox mediating capacity as evidenced by spectrophotometric screenings of the decolorized solution and the supporting material. The development of materials with enhanced both redox and adsorption properties, as the GAC used in this study, offers a promising way to increase the redox conversion of recalcitrant pollutants commonly found in industrial wastewaters.  相似文献   

Electrophilic derivatives antagonise pheromone attraction in Cydia pomonella     

Ares Mingot  Wanda García  Dolors Bosch  Josep Coll  Gloria Rosell  M Pilar Bosch  Magí Riba  Angel Guerrero 《Pest management science》2013,69(11):1280-1290

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Use of a graft of demineralized bone matrix along with TGF-β1 leads to an early bone repair in dogs     

Servin-Trujillo MA  Reyes-Esparza JA  Garrido-Fariña G  Flores-Gazca E  Osuna-Martinez U  Rodriguez-Fragoso L 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(9):1151-1161

Tibia fractures are common in small animal practice. Over the past decade, improvements to animal internal fracture fixation have been developed. TGF-β1 has been shown to be crucial in the development, induction and repair of bone. In present study, we investigate the effect of local application of a graft demineralized bone matrix (DBM) along with TGF-β1 in a model of open osteotomy induced experimentally in dogs. Tibia fracture was brought about by using an open osteotomy model in young male dogs. Fracture repair was evaluated by a histological and biochemical analysis. Collagen content, proteolytic activity and urokinase-type plasminogen activator (uPA) expression were analyzed at the end of the study. Radiographic analysis, alkaline phosphatase and hematological evaluation were performed weekly. At the fifth week, there was an improvement and restoration of bone architecture in animals treated with a graft containing TGF-β1 (5 ng/ml) compared with the control and graft groups, as was evidenced by the presence of an early formation of wide callus and bone regeneration. In addition, local application of TGF-β1 led to an increase in collagen and proteolytic activity. More immunopositive osteoclast and mesenchymal cells were found in bone tissue from animals treated with TGF-β1 as compared with the control group. No changes in alkaline phosphatase, hematological and clinical parameters were observed. This study shows that the combined use of DBM along with TGF-β1 is able to improve and accelerate the bone repair.  相似文献