Improved Microbial and Chemical Reduction of Direct Blue 71 Using Anthraquinone-2,6-disulfonate Immobilized on Granular Activated Carbon     

Luis H. Alvarez  Yair A. Del Angel  Bernardo García-Reyes 《Water, air, and soil pollution》2017,228(1):38

The aim of this study was to evaluate the redox mediating capacity of anthraquinone-2,6-disulfonate (AQDS) immobilized on granular activated carbon (GAC) during the reductive decolorization of direct blue 71 (DB71) under microbial and chemical conditions. The immobilization of AQDS on GAC was conducted by adsorption, and it has obtained an uptake capacity of 0.227 mmol g^?1. The anchorage of AQDS on GAC improved its electron transfer capacity (ETC) up to 2.05 times higher than the raw material. Similarly, the addition of GAC-AQDS increased up to 1.75- and 1.16-fold the rate of decolorization (k _d ) of DB71 under microbial and chemical conditions, respectively, in comparison to the unmodified GAC. Surprisingly, a higher k _d value was achieved in incubations without either GAC or GAC-AQDS because of the generation of aromatic amines, from the reduction DB71, taking into account that these species may act as a catalyst in the DB71 reduction process. In contrast, adsorption of aromatic amines on either GAC or GAC-AQDS decreased its redox mediating capacity as evidenced by spectrophotometric screenings of the decolorized solution and the supporting material. The development of materials with enhanced both redox and adsorption properties, as the GAC used in this study, offers a promising way to increase the redox conversion of recalcitrant pollutants commonly found in industrial wastewaters.  相似文献   

Electrophilic derivatives antagonise pheromone attraction in Cydia pomonella     

Ares Mingot  Wanda García  Dolors Bosch  Josep Coll  Gloria Rosell  M Pilar Bosch  Magí Riba  Angel Guerrero 《Pest management science》2013,69(11):1280-1290

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Use of a graft of demineralized bone matrix along with TGF-β1 leads to an early bone repair in dogs     

Servin-Trujillo MA  Reyes-Esparza JA  Garrido-Fariña G  Flores-Gazca E  Osuna-Martinez U  Rodriguez-Fragoso L 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(9):1151-1161

Tibia fractures are common in small animal practice. Over the past decade, improvements to animal internal fracture fixation have been developed. TGF-β1 has been shown to be crucial in the development, induction and repair of bone. In present study, we investigate the effect of local application of a graft demineralized bone matrix (DBM) along with TGF-β1 in a model of open osteotomy induced experimentally in dogs. Tibia fracture was brought about by using an open osteotomy model in young male dogs. Fracture repair was evaluated by a histological and biochemical analysis. Collagen content, proteolytic activity and urokinase-type plasminogen activator (uPA) expression were analyzed at the end of the study. Radiographic analysis, alkaline phosphatase and hematological evaluation were performed weekly. At the fifth week, there was an improvement and restoration of bone architecture in animals treated with a graft containing TGF-β1 (5 ng/ml) compared with the control and graft groups, as was evidenced by the presence of an early formation of wide callus and bone regeneration. In addition, local application of TGF-β1 led to an increase in collagen and proteolytic activity. More immunopositive osteoclast and mesenchymal cells were found in bone tissue from animals treated with TGF-β1 as compared with the control group. No changes in alkaline phosphatase, hematological and clinical parameters were observed. This study shows that the combined use of DBM along with TGF-β1 is able to improve and accelerate the bone repair.  相似文献   

Role of wild ruminants in the epidemiology of bluetongue virus serotypes 1, 4 and 8 in Spain     

García-Bocanegra I  Arenas-Montes A  Lorca-Oró C  Pujols J  González MA  Napp S  Gómez-Guillamón F  Zorrilla I  Miguel ES  Arenas A 《Veterinary research》2011,42(1):88

ABSTRACT: Although the importance of wild ruminants as potential reservoirs of bluetongue virus (BTV) has been suggested, the role played by these species in the epidemiology of BT in Europe is still unclear. We carried out a serologic and virologic survey to assess the role of wild ruminants in the transmission and maintenance of BTV in Andalusia (southern Spain) between 2006 and 2010.A total of 473 out of 1339 (35.3%) wild ruminants analyzed showed antibodies against BTV by both ELISA and serum neutralization test (SNT). The presence of neutralizing antibodies to BTV-1 and BTV-4 were detected in the four species analyzed (red deer, roe deer, fallow deer and mouflon), while seropositivity against BTV-8 was found in red deer, fallow deer and mouflon but not in roe deer. Statistically significant differences were found among species, ages and sampling regions. BTV RNA was detected in twenty-one out of 1013 wild ruminants (2.1%) tested. BTV-1 and BTV-4 RNA were confirmed in red deer and mouflon by specific rRT-PCR.BTV-1 and BTV-4 seropositive and RNA positive wild ruminants, including juveniles and sub-adults, were detected years after the last outbreak was reported in livestock. In addition, between the 2008/2009 and the 2010/2011 hunting seasons, the seroprevalence against BTV-1, BTV-4 and BTV-8 increased in the majority of provinces, and these serotypes were detected in many areas where BTV outbreaks were not reported in domestic ruminants. The results indicate that wild ruminants seem to be implicated in the dissemination and persistence of BTV in Spain.  相似文献   

Estimating feed efficiency: evaluation of mathematical models to predict individual intakes of steers fed in group pens     

Cruz GD  Trovo JB  Oltjen JW  Sainz RD 《Journal of animal science》2011,89(5):1640-1649

To evaluate feed efficiency using residual feed intake (RFI), it is necessary to measure and record daily feed intake for each animal. This can be accomplished by housing them in individual pens or by using sophisticated electronic feeders in group pens. All the available options are very expensive and very laborious; therefore, several researchers have developed methods to predict individual DMI of cattle fed in group pens. Three intake models were tested with a data set of 60 Angus × Hereford steers fed a corn-based finishing diet in both group and individual pens. After the first 60 d (period 1) of the study, animals were switched from group to individual pens, and then vice versa for another 60 d (period 2); thus, the entire feeding trial was 120 d long. No difference was observed in DMI between periods for steers fed individually (period 1 = 10.9 kg/d and period 2 = 11.2 kg/d, P = 0.44), but a difference was observed in group pens (period 1 = 12.7 kg/d and period 2 = 10.9 kg/d, P < 0.01). In addition, no difference (P ≥ 0.15) was observed in carcass characteristics, such as HCW, dressing percentage, quality grade, LM area, KPH percentage, yield grade, or backfat between RFI groups (low, medium, and high). Average daily gain and G:F were not different between RFI groups within each period (P ≥ 0.06), but there were period differences (P < 0.001). Models 1 and 2 were based on growth, carcass composition, and nutrient requirements, whereas model 3 was based on the heterogeneity of pen intakes when cattle were rotated through the pens on a daily basis. Models 1 and 2 were forced through the mean observed DMI, so the mean bias was zero, but they were not precise, with a slope bias greater than 50%. Model 3 showed low accuracy (mean bias = 20%), but it was precise, with a slope bias of 21%. Because RFI is the error of the DMI equation, any inaccuracy when estimating intake will lead to a bias in the prediction of RFI. In conclusion, these models could be used to predict mean DMI, but they were not adequate for estimating RFI.  相似文献   

Arsenic uptake,distribution, and accumulation in bean plants: Effect of Arsenite and salinity on plant growth and yield     

Angel A. Carbonell‐Barrachina  Francisco Burló‐Carbonell  Jorge Mataix‐Beneyto 《Journal of plant nutrition》2013,36(10):1419-1430

The response of bean plants (Phaseolus vulgaris L.) to different levels of arsenic (As) and salinity was investigated, including the processes of uptake, distribution, and accumulation of As and the effect of arsenite and salinity on plant growth and fruit production. The experiment was performed in soilless culture at two levels of As: 2 and 5 mg As L^‐1 [added as sodium arsenite (NaAsO₂)], and three saline levels [only sodium chloride (NaCl) was added]: 1,000,2,000, and 4,000 μS#lbcM^‐1. Arsenic uptake and concentration in root increased upon increased NaAsO₂ concentration in the nutrient solution. However, the increase in the As root content was not proportional to the As level in the nutrient solution. High levels of salinity in solution decreased As uptake and the concentration of As in root, stem, and leaf. Upon uptake, As was readily translocated to the aerial organs and approximately half of the absorbed As was transported to the upper parts of the bean plants. The As concentration in fruit always remained below the recommended limit for As content in fruit and edible vegetal products. While salinity did not significantly affect plant growth, arsenite was found to be phytotoxic to the bean plants.  相似文献   

Distribution and secondary effects of EDDHA in some vegetable species     

H. Frits Bienfait  Josemaria Garcia-Mina  Angel Maria Zamareño 《Soil Science and Plant Nutrition》2013,59(7):1103-1110

Tomato (Lycopersicum esculentum), cucumber (Cucumis sativus), pepper (Capsicum annuum), and lettuce (Lactuca sativa) were grown on rockwool or perlite substrate with nutrient solution. Fe was administered as the Fe complex of the chelator ethylenediamine di-(o-hydroxyphenylacetic acid) (EDDHA) or Fe(NH₄)₂(SO₄)₂ in the nutrient solution or as inorganic iron in the substrate. Roots and leaves of plants grown on Fe-EDDHA contained EDDHA in quantities up to 0.27 × the amount of Fe, which is interpreted as an indication of the contribution of passive chelate absorption to Fe uptake. Fruits of tomato and pepper, and leaves of lettuce contained only traces of EDDHA. Breakdown of the chelator in leaves of pepper and tomato is estimated to have been between 0.5 and 2% per day. In tomato fruits, lycopene content was lowered in plants growing on Fe-EDDHA. Cucumber growing on Fe-EDDHA suffered from serious infection by the mildew Sphaerotheca fusca; the plants growing on an inorganic source of iron were resistant. These results exemplify physiological effects of EDDHA other than those directly associated with iron nutrition.  相似文献   

Factorial analysis of the effect of sugarbeet vinasses on agroecological indices in horticultural field     

Miguel Angel Diez-Rojo  ;Jose Antomo Lopez-Perez  ;Maria Rosa Gonzalez-Lopez  ;Ana Piedra Buend  ;Antonio Bello  ;Gonzalo Almendros 《农业科学与技术》2009,(8):31-39

The impact on soil of applying sugarbeet vinasses （V） was analyzed through a field experiment in horticulture greenhouse, arranged in a 23 factorial design. Two levels of three independent variables--application of V, use of polyethylene cover （PC） on the soil. and soil depth （D）---on various dependent variables were studied. Vinasses favoured crop yield and reduced the number of Meloidogyne incognita juveniles in soil. The concentrations ofN. P and K increased with the interaction VxD, with PC also increasing N concentration. The amounts of humic acids and humin decreased with D; fulvic acid concentration increased with V, but decreased with the interaction VxPC. Soil physical factors were improved mainly with D and V. Aromaticity of humic acid-like fractions increased ~＇itb V. In general, V showed significant effects mainly on the topsoil, suggesting low leaching risks. The results indicate that the levels of the independent factors improving a group of variables were not the same that those contributing to another group. Therefore, their best combination should be determined for each scenario to achieve optimum agroecological performance.  相似文献   

Soil organic carbon in cropping sequences with predominance of soya bean in the argentinean humid Pampas     

Juan P. Martinez  Cecilia Crespo  Hernán Sainz Rozas  Hernán Echeverría  Guillermo Studdert  Fernado Martinez  Graciela Cordone  Pablo Barbieri 《Soil Use and Management》2020,36(1):173-183

Soya bean (Glycine max (L.) Merr.) monoculture can lead to a decrease in labile fractions of soil organic carbon (SOC). This study sought to evaluate the effects of cover crops (CC), application of fertilizer, and crop rotation on SOC, particulate organic carbon (POC), and soil carbon input in soya bean-based crop sequences under a no-till cropping system in the Argentinean Humid Pampas. Five crop sequences at two sites differing in initial SOC were evaluated: continuous soya bean (Sb), continuous soya bean fertilized with phosphorus (P) and sulphur (S) (Sbf), grass CC / PS-fertilized soya bean (CC/Sbf), nitrogen (N)-fertilized CC / PS-fertilized soya bean (CCf/Sbf) and NPS-fertilized crop rotation with high intensification sequence index (ISI) (Rot). At 0–5 cm, SOC and POC were higher (p < .05) in the sequences with higher residue-C supply (CC/Sbf; CCf/Sbf and Rot) at both sites. Changes in SOC at 0–20 cm simulated by AMG model closely tracked measured results at 0–20 cm. Findings from this study suggest that the inclusion of CC or crop rotation with high ISI improved C balance in soils under crop sequences with soya bean predominance.  相似文献   

Long-segmented filamentous organisms observed in poults experimentally infected with stunting syndrome agent     

C R Angel  J L Sell  J A Fagerland  D L Reynolds  D W Trampel 《Avian diseases》1990,34(4):994-1001

One-day-old turkeys were inoculated per os with material shown previously to induce stunting syndrome (SS). Weight gain and feed efficiency of inoculated poults from 1 to 13 days of age were impaired (P less than 0.01) compared with uninoculated poults. Examination of the jejunal mucosa by scanning and transmission electron microscopy showed the presence of long-segmented filamentous organisms (LSFOs) in poults that had been inoculated with SS. These organisms were not seen in jejuna of uninoculated poults. Further research is needed to characterize LSFOs and to determine their involvement, if any, in the adverse effects associated with SS.  相似文献