Diphenylamine residues in apples caused by contamination in fruit storage facilities     

Robatscher P  Eisenstecken D  Sacco F  Pöhl H  Berger J  Zanella A  Oberhuber M 《Journal of agricultural and food chemistry》2012,60(9):2205-2211

The potential of fruit storage facilities that are contaminated with the widely used chemical antioxidant diphenylamine to cross-contaminate untreated apples (Malus × domestica Borkh.) was studied. A new sample preparation method identified the storage room paint, contaminated from past treatments, as the major source of cross-contamination in the analyzed facilities. Diphenylamine amounts of up to 917 g were found in a single storage room and were shown to correlate with the extent of cross-contamination on stored apples. Our data support a diffusion-based mechanism where the wall paint releases the antioxidant to the storage room atmosphere even years after the last treatment. Given the extent of cross-contamination found in our model experiments and under commercial storage conditions, we deduce a significant risk of exceeding the potentially upcoming maximum residue level of 0.01 mg kg(-1) on stored fruit in contaminated rooms even years after the last diphenylamine treatment.  相似文献   

Benefits under the Sea: The Role of Marine Compounds in Neurodegenerative Disorders     

Mariano Catanesi  Giulia Caioni  Vanessa Castelli  Elisabetta Benedetti  Michele d&#x;Angelo  Annamaria Cimini 《Marine drugs》2021,19(1)

Marine habitats offer a rich reservoir of new bioactive compounds with great pharmaceutical potential; the variety of these molecules is unique, and its production is favored by the chemical and physical conditions of the sea. It is known that marine organisms can synthesize bioactive molecules to survive from atypical environmental conditions, such as oxidative stress, photodynamic damage, and extreme temperature. Recent evidence proposed a beneficial role of these compounds for human health. In particular, xanthines, bryostatin, and 11-dehydrosinulariolide displayed encouraging neuroprotective effects in neurodegenerative disorders. This review will focus on the most promising marine drugs’ neuroprotective potential for neurodegenerative disorders, such as Parkinson’s and Alzheimer’s diseases. We will describe these marine compounds’ potential as adjuvant therapies for neurodegenerative diseases, based on their antioxidant, anti-inflammatory, and anti-apoptotic properties.  相似文献   

Metal Tolerance, Accumulation and Translocation in Poplar and Willow Clones Treated with Cadmium in Hydroponics   总被引：3，自引：0，他引：3  

Massimo Zacchini  Fabrizio Pietrini  Giuseppe Scarascia Mugnozza  Valentina Iori  Lucia Pietrosanti  Angelo Massacci 《Water, air, and soil pollution》2009,197(1-4):23-34

To evaluate the phytoremediation capability of some poplar and willow clones a hydroponic screening for cadmium tolerance, accumulation and translocation was performed. Rooted cuttings were exposed for 3 weeks to 50 μM cadmium sulphate in a growth chamber and morpho-physiological parameters and cadmium content distribution in various parts of the plant were evaluated. Total leaf area and root characteristics in clones and species were affected by cadmium treatment in different ways. Poplar clones showed a remarkable variability whereas willow clones were observed to be more homogeneous in cadmium accumulation and distribution. This behaviour was further confirmed by the calculation of the bio-concentration factor (BCF) and the translocation factor (Tf). Mean values of all the clones of the two Salicaceae species showed that willows had a far greater ability to tolerate cadmium than poplars, as indicated by the tolerance index (Ti), calculated on the dry weight of roots and shoots of plants. As far as the mean values of Tf was concerned, the capacity of willows to translocate was double that of poplars. On the contrary, the mean values of total BCF in poplar clones was far higher with respect to those in willows. The implications of these results in the selection of Salicaceae clones for phytoremediation purposes were discussed.  相似文献   

Pseudomonas syringae pv. Actinidiae isolated from non-kiwifruit plant species in China     

Pu Liu  Shizhou Xue  Rong He  Jiayong Hu  Xiaojie Wang  Bing Jia  Lorenzo Gallipoli  Angelo Mazzaglia  Giorgio M. Balestra  Liwu Zhu 《European journal of plant pathology / European Foundation for Plant Pathology》2016,145(4):743-754

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Early adult learning affects host preferences in the tephritid parasitoid Psyttalia concolor (Hymenoptera: Braconidae)     

Giulia Giunti  Giovanni Benelli  Russell H. Messing  Angelo Canale 《Journal of pest science》2016,89(2):529-537

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Metschnikowia pulcherrima strain MACH1 outcompetes Botrytis cinerea, Alternaria alternata and Penicillium expansum in apples through iron depletion     

Duraisamy Saravanakumar  Annalisa Ciavorella  Davide Spadaro  Angelo Garibaldi  M. Lodovica Gullino 《Postharvest Biology and Technology》2008,49(1):121-128

A new strain of Metschnikowia pulcherrima (MACH1) was studied for its efficacy as a biocontrol agent against Botrytis cinerea, Penicillium expansum and Alternaria alternata on apples stored for 8 months at 1 °C. The results of two semi-commercial trials demonstrated the efficacy of the biocontrol strain MACH1. In order to understand the mechanism of action involved, the yeast strain was investigated for its competitive ability for iron against postharvest pathogens of apple. M. pulcherrima strain MACH1 was cultivated on PDA with different concentrations of iron (supplemented as FeCl₃) against A. alternata and B. cinerea. The yeast strain MACH1 produced a wider pigmented inhibition zone against both pathogens in low iron amendments while less inhibition was measured with increased iron concentrations. At the coloured inhibition zone, B. cinerea and A. alternata conidia did not germinate and mycelial degeneration was observed. In addition, a high reduction in infection by both pathogens was recorded in apples treated with M. pulcherrima strain MACH1 supplemented with low iron amendments compared to higher iron concentrations. The same experiments were carried out in vivo and in vitro against P. expansum. M. pulcherrima strain MACH1 amended with low iron concentration (5 μg mL⁻¹ FeCl₃), showing modest lesion diameter reduction and no effect on P. expansum under increased iron and without iron amendments. This study illustrated that iron depletion by the yeast strain MACH1 under low iron conditions could reduce the growth of some postharvest pathogens in vitro and in vivo. Although, iron depletion seems to be a primary mode of action against the postharvest pathogens studied, other mechanisms of action cannot be excluded in the biocontrol employed by M. pulcherrima strain MACH1.  相似文献   

A recombinant flagellin fragment,which includes the epitopes flg22 and flgII-28, provides a useful tool to study flagellin-triggered immunity     

Serena Ciarroni  Christopher R. Clarke  Haijie Liu  Noam Eckshtain-Levi  Angelo Mazzaglia  Giorgio M. Balestra  Boris A. Vinatzer 《Journal of General Plant Pathology》2018,84(3):169-175

Plants and animals independently evolved the ability to recognize flagellin (also called FliC), the building block of the bacterial flagellum, as part of their innate immune response. While animals recognize a relatively large region of FliC, most plants recognize one or two short epitopes of FliC: flg22 and flgII-28. However, since most research in plants has focused on flg22 and flgII-28 and not the actual FliC protein, the importance of any FliC region beyond the two epitopes in plant immunity is poorly understood. Here we report cloning, overexpression, and purification of a Pseudomonas syringae FliC fragment from amino acid 1 to 143, which includes both FliC epitopes and the adjacent alpha helices. Exposing Arabidopsis thaliana leaves to FliC_1–143 did not reveal any additional FliC recognition capabilities beyond flg22. However, while the kiwifruit species Actinidia arguta did not respond to either flg22 or flgII-28, treatment of A. arguta leaves with FliC_1–143 triggered a significant reactive oxygen response, indicating recognition. This result suggests that in some plant species, recognition of FliC requires regions of FliC beyond the two well-known epitopes and that FliC_1–143 represents a useful tool in the study of plant immunity.  相似文献   

Cytology of the healthy canine and feline ocular surface: comparison between cytobrush and impression technique          下载免费PDF全文

Anna Perazzi  Federico Bonsembiante  Maria Elena Gelain  Marco Patruno  Enzo Di Iorio  Angelo Migliorati  Ilaria Iacopetti 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2017,46(1):164-171

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Natural antimicrobials in spray-dried microparticles based on cellulose derivatives as potential eco-compatible agrochemicals     

Cortesi  Rita  Quattrucci  Alessio  Esposito  Elisabetta  Mazzaglia  Angelo  Balestra  Giorgio M. 《植物病害和植物保护杂志》2017,124(3):269-278

Journal of Plant Diseases and Protection - It has been demonstrated that some aromatic substances synthesized by plants serve as plant defense mechanisms. However, natural extracts are difficult to...  相似文献   

Efficacy of different steam distribution systems against five soilborne pathogens under controlled laboratory conditions     

Pingxiang Lu  Davide Ricauda Aimonino  Giovanna Gilardi  Maria Lodovica Gullino  Angelo Garibaldi 《Phytoparasitica》2010,38(2):175-189

The efficacy of three steam application techniques (steam injection, iron pan and sheet steaming) was evaluated against five soilborne pathogens under controlled laboratory conditions. Injection and pan steam systems proved to be efficient and feasible alternatives to traditional sheet steaming for suppressing Fusarium oxysporum f. sp. basilici at 60% moisture field capacity in sandy-loam soil. Injecting steam was the best technique to suppress F. oxysporum f. sp. basilici, F. oxysporum f. sp. raphani, F. oxysporum f. sp. conglutinans, Rhizoctonia solani and Phytophthora capsici. The mycelia of R. solani and P. capsici were very sensitive to heat and were effectively killed by injection of steam and by the pan steam system at 80% and 40% moisture field capacity.  相似文献