Agglutinins to Brucella canis in stray dogs from certain counties in Illinois and Wisconsin.     

F W Boebel  F A Ehrenford  G M Brown  R D Angus  C O Thoen 《Journal of the American Veterinary Medical Association》1979,175(3):276-277

In a serologic and blood culture survey for Brucella canis in 2,572 pound dogs from eight Illinois and Wisconsin counties, 173 (6.7%) were reactive in a presumptive slide test, 41 (1.5%) were reactive in a tube agglutination test, and 6 (0.2%) were positive in blood culture.  相似文献   

Otic cytology in health and disease.   总被引：1，自引：0，他引：1  

John C Angus 《Veterinary Clinics of North America: Small Animal Practice》2004,34(2):411-424

Accurate characterization of the primary cause and perpetuating factors is essential for successful management of ear disease in dogs and cats. Cytology is a simple, rapid, and practical diagnostic test that should be performed routinely on any and all patients presented for clinical signs consistent with otitis externa. In combination with clinical signs, otoscopic evaluation, and diagnostic testing of primary disease, serial cytology enhances the ability of veterinarians to diagnose secondary infections, monitor progression of disease, evaluate response to therapy, and make appropriate management decisions. Cytologic specimens should be evaluated for the presence, numbers, and characteristics of three key features: yeast, bacteria, and leukocytes. More than five yeast organisms or more than 25 bacteria per high-powered field is suggestive of significant microbial activity warranting therapeutic intervention. The presence of leukocytes, particularly with phagocytized bacteria, indicates "true infection" rather than overgrowth; if suppurative discharge is present, systemic therapy is needed. Cytology combined with culture and susceptibility is the best method for identification of bacterial overgrowth and infection; however, if only one test can be performed, always choose cytology. Culture results assist in the selection of appropriate antibiotic therapy, but cytology determines whether systemic antibiotics are indicated, which organisms are most significant, and when therapy can be discontinued.  相似文献   

A practical approach to calculate sample size for herd prevalence surveys   总被引：1，自引：0，他引：1  

Humphry RW  Cameron A  Gunn GJ 《Preventive veterinary medicine》2004,65(3-4):173-188

When designing a herd-level prevalence study that will use an imperfect diagnostic test, it is necessary to consider the test sensitivity and specificity. A new approach was developed to take into account the imperfections of the test. We present an adapted formula that, when combined with an existing piece of software, allows improved planning. Bovine paratuberculosis is included as an example infection because it originally stimulated the work. Examples are provided of the trade-off between the benefit (low number of herds) and the disadvantage (large number of animals per herd and exclusion of small herds) that are associated with achieving high herd-level sensitivity and specificity. We demonstrate the bias in the estimate of prevalence and the underestimate of the confidence range that would arise if we did not account for test sensitivity and specificity.  相似文献   

FELLOWS OF THE AUSTRALIAN VETERINARY ASSOCIATION     

Angus Packham  Donald Thomas Oxer 《Australian veterinary journal》1969,45(9):428-429

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Development of immunity to incoming radiolabelled larvae in lambs continuously infected with Ostertagia circumcincta   总被引：2，自引：0，他引：2  

D S Seaton  F Jackson  W D Smith  K W Angus 《Research in veterinary science》1989,46(2):241-246

Infective larvae of Ostertagia circumcincta were radiolabelled with 75selenium by a method which did not affect their viability. Three groups of five-month-old lambs were infected daily with 1000 unlabelled infective larvae for four, eight and 12 weeks, respectively. After each period one of these groups and a group of worm-free controls were challenged with three consecutive daily doses of 1000 radiolabelled third stage larvae. The lambs were killed 13 days after the first dose of challenge larvae and their worm burdens examined. The first indication of immunity was retardation of developing worms observed at four weeks. Resistance to the establishment of incoming worms developed between four and eight weeks and a brief period of population turnover probably took place at this time. Simultaneously a greater inhibition of worm development occurred resulting in an increase in the number of parasites recovered as early fourth stage larvae. By 12 weeks the animals were almost completely immune to incoming worms. The development of resistance to incoming worms correlated with a rise in serum antibody titre and an increase in the number of intraepithelial globule leucocytes in the gastric mucosa.  相似文献   

Kinetics of in vitro bovine lymphocyte immunostimulation with a Brucella abortus antigen.     

J M Kaneene  D W Johnson  R K Anderson  R D Angus  D E Pietz  C C Muscoplat 《American journal of veterinary research》1978,39(2):235-239

A Brucella abortus-soluble antigen was investigated, using in vitro assay of lymphocyte immunostimulation, to determine which concentration of this antigen and which period of incubation of the lymphocyte cultures would induce maximum specific lymphocyte immunostimulation as an additional method for further study of B abortus infection in cattle. Soluble antigen was prepared from autoclaved cells of B abortus strain 1119-3. Peripheral blood lymphocytes were obtained from cattle infected with B abortus and from healthy control cattle not infected with B abortus. The lymphocytes were prepared by the Ficoll-Hypaque density gradient technique, suspended in RPMI 1640 medium (1.5 X 10(6)/ml), cultured with several dilutions of soluble antigen, and incubated. Prior to termination of incubation, cultures were labeled with 1 muCi of [3H]thymidine and, after harvesting, assayed for [3H]thymidine incorporation in DNA by a liquid scintillation spectrometer. Maximum specific immunostimulation of lymphocytes from B abortus-infected cattle was induced in this assay system with 6 days' incubation and 22 microgram of protein/ml/1.5 X 10(6) lymphocytes, using protein content to express concentration of soluble antigen in this system.  相似文献   

Effects of land-cover change on spatial pattern of forest communities in the Southern Appalachian Mountains (USA)   总被引：8，自引：0，他引：8  

Turner  Monica G.  Pearson  Scott M.  Bolstad  Paul  Wear  David N. 《Landscape Ecology》2003,18(5):449-464

Landscape Ecology - Understanding the implications of past, present and future patterns of human land use for biodiversity and ecosystem function is increasingly important in landscape ecology. We...  相似文献   

Behavioral and clinical signs of Chiari‐like malformation‐associated pain and syringomyelia in Cavalier King Charles spaniels     

Clare Rusbridge  Angus K. McFadyen  Susan P. Knower 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2019,33(5):2138-2150

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Compliance between clinical and genetic diagnosis of choroidal hypoplasia in 103 Norwegian Border Collie puppies          下载免费PDF全文

Siv Grosås  Frode Lingaas  Kristin Wear Prestrud  Ernst‐Otto Ropstad 《Veterinary ophthalmology》2018,21(4):371-375

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Microbiological and histopathological features of canine acral lick dermatitis     

Shumaker AK  Angus JC  Coyner KS  Loeffler DG  Rankin SC  Lewis TP 《Veterinary dermatology》2008,19(5):288-298

The purpose of this study was to investigate microbiological and histopathological features of canine acral lick dermatitis (ALD). Microbial characteristics of ALD are poorly described in current literature. If infection is recognized, antimicrobial selection is usually empirical, based on appearance, cytology or surface culture, rather than deep tissue culture. It was hypothesized that cultures obtained from deep tissue would yield different results than predicted by surface culture and cytology, and that isolates from ALD have unpredictable susceptibility patterns showing resistance to antibiotics routinely administered for canine pyoderma. Biopsies were obtained from 31 lesions and submitted for aerobic, anaerobic and fungal culture, and histopathological evaluation. Surface aerobic culture and susceptibility and cytology were obtained for comparison in 22 dogs. Skin scrapings and dermatophyte culture were performed. Bacteria were isolated in 30 of 31 cases. Staphylococcus intermedius was isolated in 58% of deep cultures. Twenty per cent of deep isolates were methicillin-resistant Staphylococcus species. Forty-eight per cent of cases yielded organisms defined as multidrug resistant on deep culture. Only 57% and 55% of bacteria isolated from tissue culture were sensitive to amoxicillin-clavulanic acid and cefazolin, respectively. Cytology and superficial cultures did not correlate well with deep cultures. Surface culture predicted deep tissue isolates in eight of 22 cases. Microsporum gypseum was isolated from one dog. Histopathological features included acanthosis, follicular elongation, lymphoplasmacytic dermal inflammation, folliculitis, furunculosis, perihidradenitis, hidradenitis and vertical streaking fibrosis. Lesions associated with ALD warrant tissue bacterial cultures as the majority of cases yielded positive growth of bacteria differing from superficial culture and often resistant to empirical drugs.  相似文献