Effect of inoculation with wild type Azospirillum brasilense and A. irakense strains on development and nitrogen uptake of spring wheat and grain maize     

Sofie Dobbelaere  Anja Croonenborghs  Amber Thys  David Ptacek  Yaacov Okon  Jos Vanderleyden 《Biology and Fertility of Soils》2002,36(4):284-297

Under the controlled conditions of the greenhouse and by varying some biotic and abiotic factors, we tried to identify some of the factors critical to obtain successful Azospirillum inoculation. Spring wheat and grain maize were inoculated with different concentrations of the wild type strains A. brasilense Sp245 and A. irakense KBC1, and grown in a substrate with varying concentrations of organic matter (OM) and N fertiliser. The inoculum concentration was one of the factors that influenced most the outcome of an inoculation experiment on wheat, with lower inoculum concentrations (10⁵-10⁶ cfu plant^-1) stimulating root development and plant dry weight and higher inoculum concentrations (10⁷-10⁸ cfu plant^-1) having no effect or sometimes even inhibiting root development. The effect of inoculation was most pronounced at low to intermediate N fertilisation levels, while the OM content of the substrate had no effect. Inoculation was found to affect early plant and root development, plant and root dry weight, grain yield and the N-uptake efficiency of plants. However, inoculation did not change the N concentration in plants or grains. In addition, a difference in the ability of both strains to stimulate plant growth and N uptake of wheat and maize was observed, with A. brasilense Sp245 having most effect on spring wheat and A. irakense KBC1 being more effective on grain maize. The significance of the obtained results for agriculture is discussed.  相似文献   

The influence of diet on Lawsonia intracellularis colonization in pigs upon experimental challenge     

Boesen HT  Jensen TK  Schmidt AS  Jensen BB  Jensen SM  Møller K 《Veterinary microbiology》2004,103(1-2):35-45

The objective of this investigation was to study if different feeding strategies influence experimental infections of pigs with Lawsonia intracellularis, the causative agent of proliferative enteropathy. In three sequential trials, a total of 144 weaned pigs were fed five different diets all made from a standard diet based on wheat and barley as carbohydrate source and soybean as protein source. The five diets were: a standard diet (fine ground and pelleted), the standard diet fed as fermented liquid feed, the standard diet added 1.8% formic acid, the standard diet added 2.4% lactic acid and a diet similar to the standard diet (made from the same ingredients), but fed coarse ground. Twenty-four pigs on each diet were orally inoculated with L. intracellularis and growth performance and faecal excretion of bacteria were monitored. Twenty-four pigs fed the standard diet were included as not experimentally infected controls. Pigs in the first two trials were sacrificed 4 weeks post-inoculation, whereas animals in the third trial were sacrificed after 5 weeks. Pigs in all experimentally infected groups excreted L. intracellularis. The fermented liquid diet delayed the excretion of L. intracellularis and furthermore, pigs fed the standard diet supplemented with lactic acid had limited pathological lesions when the intestines were examined 4 weeks after inoculation. The growth performance was reduced in pigs experimentally challenged with L. intracellularis, however the prevalence and severity of diarrhea was limited.  相似文献   

Escherichia coli Shiga toxin 1 enhances il-4 transcripts in bovine ileal intraepithelial lymphocytes     

Moussay E  Stamm I  Taubert A  Baljer G  Menge C 《Veterinary immunology and immunopathology》2006,113(3-4):367-382

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Diabetes: what about patterns, fruits, and vegetables?     

Kroke A 《Public health nutrition》2006,9(2):276

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Aneuploidy and isochromosome formation in drug-resistant Candida albicans     

Selmecki A  Forche A  Berman J 《Science (New York, N.Y.)》2006,313(5785):367-370

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Clinical efficacy of the acyclic nucleoside phosphonate 9-(2-phosphonylmethoxypropyl)-2,6-diaminopurine (PMPDAP) in the treatment of feline immunodeficiency virus-infected cats     

Hartmann AD  Wilhelm N  Balzarini J  Hartmann K 《Journal of Feline Medicine and Surgery》2012,14(2):107-112

In in vitro studies, the acyclic nucleoside phosphonate 9-(2-phosphonylmethoxypropyl)-2,6-diaminopurine (PMPDAP) inhibited the replication of feline immunodeficiency virus (FIV). No information about its clinical efficacy is available so far. The aim of this prospective placebo-controlled, double-blinded study was to evaluate the antiviral efficacy of PMPDAP in cats naturally infected with FIV. Twenty cats were randomly assigned to two treatment groups receiving either PMPDAP (25 mg/kg) or placebo twice per week subcutaneously for 6 weeks. The general health status (Karnofsky's score), clinical signs, laboratory, immunological, and surrogate parameters were evaluated. No significant differences were found between PMPDAP- and placebo-treated cats, although cats treated with PMPDAP showed a tendency for improvement in their Karnofsky's score and clinical signs. Haematological side effects were noted in the PMPDAP-treated cats. Thus, PMPDAP may be an option in treating cats if it becomes available for veterinarians, but side effects have been monitored.  相似文献   

PMN-mediated immune reactions against Eimeria bovis     

Behrendt JH  Hermosilla C  Hardt M  Failing K  Zahner H  Taubert A 《Veterinary parasitology》2008,151(2-4):97-109

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Assessment of in vitro interferon-gamma responses from peripheral blood mononuclear cells of cattle infected with Mycoplasma mycoides ssp. mycoides small colony type     

Jores J  Nkando I  Sterner-Kock A  Haider W  Poole J  Unger H  Muriuki C  Wesonga H  Taracha EL 《Veterinary immunology and immunopathology》2008,124(1-2):192-197

Contagious bovine pleuropneumonia (CBPP) is a lung disease caused by the bacterial pathogen Mycoplasma mycoides ssp. mycoides small colony type (MmmSC). It has been spreading due to a number of factors including poor vaccine efficacy and poor sensitivity of current diagnostic tests. The purpose of this study was to assess interferon gamma (IFN-gamma) release after stimulation of peripheral blood mononuclear cells (PBMC) from experimentally infected cattle. PBMC collected from 15 artificially infected animals were incubated with different concentrations of total MmmSC antigen. After 72h of incubation the IFN-gamma release was measured and found to be elevated in 11 animals. We did not observe a correlation between IFN-gamma release of animals with and without pathomorphological gross lesions. Therefore, our data do not confirm a role for CD4 T-lymphocytes in protection, since there is no correlation between IFN-g secretion (supposed to be mainly derived from CD4 T-cells) and disease severity. Additionally, we applied immunocytochemistry on affected lung tissue and detected no build up of T-lymphocytes (CD4 T-cells, CD8 T-cells) but a high presence of myeloid cells.  相似文献   

Retrospective analysis of ocular neuropathies in diabetic dogs following cataract surgery     

Braidee C. Foote  Tammy M. Michau  Anja Welihozkiy  Jessica M. Stine 《Veterinary ophthalmology》2019,22(3):284-293

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Retinal detachment and glaucoma in the Boston Terrier and Shih Tzu following phacoemulsification (135 patients): 2000–2014          下载免费PDF全文

Braidee C. Foote  Samantha L. Pederson  Anja Welihozkiy  Jessica M. Stine  Susan M. Carastro  Stacy E. Andrew  Tammy M. Michau 《Veterinary ophthalmology》2018,21(3):240-248

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