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11.
Bertoli A Giovannini A Ruffoni B Guardo AD Spinelli G Mazzetti M Pistelli L 《Journal of agricultural and food chemistry》2008,56(13):5078-5082
A wild strain of Agrobacterium rhizogenes was used to regenerate twelve in vitro plant lines from different hairy roots of H. perforatum (St. John's Wort). The production of the main bioactive constituents was observed even though their yields varied in the different plant lines. Two lines were selected for the hyperoside production (4.9-4.6 mg/gdw) while nine were characterized by significant yields of chlorogenic acid (ranged from 0.47 to 1.09 mg/gdw). Furthermore, one out of twelve lines showed a 10-fold higher hypericin content (0.25 mg/gdw) than that reported for the in vitro shoots in the literature. Morphological and phytochemical features were determined in order to select H. perforatum genotypes enriched in valuable bioactive compounds. 相似文献
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Anna Esposito Daniele D&#x;Alonzo Stefano D&#x;Errico Eliana De Gregorio Annalisa Guaragna 《Marine drugs》2020,18(11)
In the effort to improve the antimicrobial activity of iminosugars, we report the synthesis of lipophilic iminosugars 10a–b and 11a–b based on the one-pot conjugation of both enantiomeric forms of N-butyldeoxynojirimycin (NBDNJ) and N-nonyloxypentyldeoxynojirimycin (NPDNJ) with cholesterol and a succinic acid model linker. The conjugation reaction was tuned using the established PS-TPP/I2/ImH activating system, which provided the desired compounds in high yields (94–96%) by a one-pot procedure. The substantial increase in the lipophilicity of 10a–b and 11a–b is supposed to improve internalization within the bacterial cell, thereby potentially leading to enhanced antimicrobial properties. However, assays are currently hampered by solubility problems; therefore, alternative administration strategies will need to be devised. 相似文献
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Romani A Vignolini P Isolani L Ieri F Heimler D 《Journal of agricultural and food chemistry》2006,54(4):1342-1346
Flavonoids and hydroxycinnamic derivatives of turnip tops (Brassica rapa L. subsp. sylvestris L.) were characterized for the first time in four samples from different origins. Turnip tops exhibit a high polyphenols content (ranging from 107 to 191 mg/100 g, fresh weight) and a good antiradical activity, determined with the DPPH* test. After a liquid-liquid extraction and fractionation procedures, most flavonoids (isorhamnetin, kaempferol, and quercetin glycosides) and hydroxycinnamic derivatives were identified by means of HPLC-DAD/MS techniques. Isorhamnetin glycosides were the main flavonoid derivatives, differing from that found in the vegetables belonging to the Brassica oleracea group. 相似文献
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Elena Fringuelli Lorena Urbanelli Omar Tharuni Patrizia Casagrande Proietti Annalisa Bietta Irit Davidson Maria Pia Franciosini 《Research in veterinary science》2010,89(3):367-372
This is the first paper describing the cloning of pigeon IFN-γ gene (PiIFN-γ) and the analysis of the in vitro expressed recombinant protein. The PiIFN-γ gene was identified by RT-PCR as a 498 bp, fragment coding for a precursor protein of 165 amino acids instead of 164 amino acids, as observed in the other avian species. The recombinant protein was expressed in vitro by an eukaryotic system and the biological properties of the cytokine were tested using a chicken macrophage cell line. The high degree of amino acid and nucleotide identity, shared with the ChIFN-γ, and the fact that the pigeon protein was functional on chicken cells, indicates a cross-reactivity between pigeon and chicken IFN-γ. The detection of the PiIFN-γ could represent an useful instrument in understanding the role played by this cytokine in immune response related to vaccinations and infectious diseases in the pigeon. 相似文献
15.
Patrizia Casagrande Proietti Annalisa Bietta Chiara Brachelente Elvio Lepri Irit Davidson Maria Pia Franciosini 《Journal of veterinary science (Suw?n-si, Korea)》2010,11(3):221-225
The aim of this study was to evaluate the presence of Helicobacter (H.) spp. in swine affected by gastric ulceration. Stomachs from 400 regularly slaughtered swine were subjected to gross pathological examination to evaluate the presence of gastric ulcers. Sixty-five samples collected from ulcerated pars esophagea and 15 samples from non-ulcerated pyloric portions were submitted to histopathological and molecular analyses, to detect Helicobacter spp., H. suis and H. pylori by PCR. Feces and saliva swabs were also collected from 25 animals in order to detect in vivo the presence of Helicobacter spp.. Gastric ulcers were detected in 373 cases (93%). The presence of ulcers in association with inflammatory processes was further confirmed by histological examination. Forty-nine percent (32/65) of the ulcerated esophageal portions as well as 53% (8/15) of the non-ulcerated pyloric portions were positive for Helicobacter spp. by PCR. The Helicobacter spp. positive samples were also positive for H. suis, while H. pylori was not detected. These results were confirmed by restriction enzyme analysis. With regard to feces and saliva samples, 15/25 (60%) and 16/25 (64%) were positive for Helicobacter spp. PCR, respectively but all were negative in H. suis and H. pylori specific PCR. 相似文献
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Gianluca De Rinaldis Antonella Leone Stefania De Domenico Mar Bosch-Belmar Rasa Slizyte Giacomo Milisenda Annalisa Santucci Clara Albano Stefano Piraino 《Marine drugs》2021,19(9)
Increasing frequency of native jellyfish proliferations and massive appearance of non-indigenous jellyfish species recently concur to impact Mediterranean coastal ecosystems and human activities at sea. Nonetheless, jellyfish biomass may represent an exploitable novel resource to coastal communities, with reference to its potential use in the pharmaceutical, nutritional, and nutraceutical Blue Growth sectors. The zooxanthellate jellyfish Cassiopea andromeda, Forsskål, 1775 (Cnidaria, Rhizostomeae) entered the Levant Sea through the Suez Canal and spread towards the Western Mediterranean to reach Malta, Tunisia, and recently also the Italian coasts. Here we report on the biochemical characterization and antioxidant activity of C. andromeda specimens with a discussion on their relative biological activities. The biochemical characterization of the aqueous (PBS) and hydroalcoholic (80% ethanol) soluble components of C. andromeda were performed for whole jellyfish, as well as separately for umbrella and oral arms. The insoluble components were hydrolyzed by sequential enzymatic digestion with pepsin and collagenase. The composition and antioxidant activity of the insoluble and enzymatically digestible fractions were not affected by the pre-extraction types, resulting into collagen- and non-collagen-derived peptides with antioxidant activity. Both soluble compounds and hydrolyzed fractions were characterized for the content of proteins, phenolic compounds, and lipids. The presence of compounds coming from the endosymbiont zooxanthellae was also detected. The notable yield and the considerable antioxidant activity detected make this species worthy of further study for its potential biotechnological sustainable exploitation. 相似文献
18.
Emergence of cutaneous neosporosis in a dog receiving immunosuppressive therapy: molecular identification and management 
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Annalisa M. Hernandez Graham E. A. Bilbrough Dennis B. DeNicola Celine Myrick Suzanne Edwards Jeremy M. Hammond Alex N. Myers Johanna C. Heseltine Karen Russell Marco Giraldi Mary B. Nabity 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2019,33(1):167-177
20.
Luca Pipino Marie-Christine Van Labeke Andrea Mansuino Valentina Scariot Annalisa Giovannini Leen Leus 《Euphytica》2011,178(2):203-214
Fertility of hybrid tea roses is often reduced due to their interspecific origin but also to intensive inbreeding. New genotypes
used as pollen donors represent an economic risk for a breeding programme, as their influence on seed production is unknown.
In this study 11 cut rose genotypes were selected from a company database as high fertile or low fertile male parents, according
to the number of seeds per hybridisation. Pollen morphology and in vitro germination of the selected genotypes were characterised.
Pollen was either small (mean diameter <30 μm), shrunken, and irregular (abnormal), or large (mean diameter >30 μm), elliptical
and crossed by furrows (normal). High correlations were found between the number of seeds produced per hybridisation and the
pollen diameter (r = 0.94) or the percentage of normal pollen (r = 0.96). In order to evaluate the predictive power of the models, we conducted regression analyses and performed a validation
experiment on genotypes not present in the database and without background information on fertility. Pollen diameter and percentage
of normal pollen were characterised and fitted in the regression models for seed set predictions. Validation with an independent
dataset gave a good prediction for 83.3% of the data. This indicates that using either the mean pollen diameter or the percentage
of normal pollen resulted in effective fertility prediction. This tool could enhance the genetic variability in crossings
between hybrid tea roses, thus creating possibilities for less economically risky exploitation of new tetraploid genotypes
as male parents. 相似文献