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41.
Scagliarini A Dal Pozzo F Gallina L Guercio A Vaccari F Battilani M Ciulli S Prosperi S 《Veterinary microbiology》2006,114(1-2):142-147
Proliferative orf virus infections in adult sheep have increased in Italy in the past few years: these extreme cases are frequently fatal and difficult to differentiate from other infectious diseases of sheep such as blue tongue. A probable explanation for the proliferative and highly vascularized nature of the lesions was found in the expression of the VEGF-E gene encoded by the orf virus. To investigate a possible role of the viral VEGF in the pathogenesis of severe persistent orf virus lesions, the activity of four VEGF-E variants was compared by an angiogenesis in vitro model. Similar angiogenic activity was found between strains isolated from the classical and the proliferative forms of the disease, even if the latter was able to develop a higher number of vessels during the first 24 h of infection. Our in vitro findings seems to exclude that the VEGF variants encoded by the strain isolated from the atypical form of the disease could be the responsible for the histopathological aspect of the proliferative lesions. 相似文献
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Braconi D Sotgiu M Millucci L Paffetti A Tasso F Alisi C Martini S Rappuoli R Lusini P Sprocati AR Rossi C Santucci A 《Journal of agricultural and food chemistry》2006,54(8):3163-3172
Herbicides are released to the environment with potential ecotoxicological risks for mammals. Yeast is a good model to elucidate toxicity mechanisms. We investigated how three commercial herbicides (Proper Energy, Pointer, and Silglif) and their active ingredients (respectively, fenoxaprop-P-ethyl, tribenuron methyl, and glyphosate) can affect biological activities of an oenological Saccharomyces cerevisiae strain, which may be resident on grape vineyards of the same geographical areas where herbicides are used. The use of commercial grade herbicides employed in Italy allowed us to reproduce the same conditions applied in crops; at the same time, assaying pure single active compounds made it possible to compare the effects obtained with commercial formulations. Interestingly, we found that while pure active compounds affect cell growth and metabolism at a lower extent, commercial preparations have a significant major negative influence on yeast biology. 相似文献
43.
Heimler D Isolani L Vignolini P Tombelli S Romani A 《Journal of agricultural and food chemistry》2007,55(5):1724-1729
Ten genotypes belonging to Lactuca sativa, Cicorium intybus, Plantago coronopus, Eruca sativa, and Diplotaxis tenuifolia and used in fresh mixed salads were investigated for their polyphenol contents. Flavonoids and hydroxycinnamic acids were characterized by high-performance liquid chromatography (HPLC)/diode array detection/mass spectrometry. Quercetin, kaempferol, luteolin, apigenin, and crysoeriol derivatives were identified; hydroxycinnamic acids were all caffeoyl derivatives. The total polyphenol content was obtained through the Folin-Ciocalteu test and from the HPLC data. The amounts ranged between 0.9 and 4.7 mg/g fresh weight. The antiradical activity was determined by the reaction with the stable DPPH* radical. The Fe2+ chelating activity was determined with a spectrophotometric test. From the complex of data, a quite complete picture of the characteristics of the vegetables emerges. A cultivated C. intybus cultivar exhibited the highest polyphenol content, while a wild C. intybus genotype exhibited the highest antiradical activity. In every case, the characteristics of the different salads as functional foods have been pointed out. 相似文献
44.
Romani A Lapucci C Cantini C Ieri F Mulinacci N Visioli F 《Journal of agricultural and food chemistry》2007,55(4):1315-1320
We characterized "Olivastra Seggianese" extra virgin olive oil (EVOO) and evaluated its chemical and sensory characteristics and antioxidant and antiradical activities during storage under novel conditions. Two oils (A and B) were analyzed for the commodity characteristics at blending (t0) and after 9, 12, and 18 months; panel tests were performed and minor polar compounds (MPC) content was assessed at blending (t0) and after 6, 9, 12, and 18 months. Antioxidant and antiradical activities in vitro were evaluated at t0 and after 12 months, by human low density lipoprotein (LDL) and 1,1-diphenyl-2-picrylhydrazil radical (DPPH*) tests. Oil A, which had an initially higher MPC content, possessed "harder" organoleptic characteristics than oil B, which had a lower MPC content and was endowed with a "smoother" taste profile. Statistical analyses showed that secoiridoids, particularly deacetoxy-oleuropein aglycone, should be quantified to evaluate EVOO stability during storage. The antioxidant activity toward human LDL was linked to MPC content and to storage time. The tests on the stable free radical DPPH* confirmed the results on human LDL. We propose this as an additional parameter to evaluate olive oil quality and stability over time. 相似文献
45.
Duranti M Di Cataldo A Sessa F Scarafoni A Ceciliani F 《Journal of agricultural and food chemistry》2002,50(7):2029-2033
The susceptibility to trypsin of conglutin gamma, a lupin seed glycoprotein affected by this enzyme only when in a non-native conformation, was used to study the effect of Zn(2+) and other metal ions on the structural dynamics of the protein. When acid-treated trypsin-susceptible conglutin gamma was incubated at neutral pH in the presence of Zn(2+), it became resistant to tryptic attack, contrary to the protein treated in the absence of Zn(2+). The time course of this refolding event has been quantitatively evaluated by SDS-PAGE. Amino acid sequencing of the major polypeptide fragments, produced by trypsin before completion of the refolding process, indicated that only a few cleavable bonds were accessible to the enzyme. This suggested that the presence of metal ions affected the pathway of degradation of the protein, by inducing its folding. Among the other metal ions tested, Ni(2+) also promoted the adoption of a trypsin-resistant conformation of conglutin gamma, whereas Mn(2+) and Ca(2+) had only much lower effects. The relevance of these findings for a deeper understanding of the in vivo degradation of plant food proteins and how it is affected by metal ions are discussed. 相似文献
46.
De Girolamo A Solfrizzo M Vitti C Visconti A 《Journal of agricultural and food chemistry》2004,52(21):6478-6484
The occurrence of 6-methoxymellein (6-MM) in fresh and conventionally processed carrot products (for a total of 176 samples) marketed in European locations and the effect of Alternaria spp. infection and storage conditions on 6-MM accumulation were investigated. 6-MM was found in 78% of tested samples with levels ranging from 0.02 to 76.00 microg/g, with only 1 of 79 fresh carrots exceeding the "just noticeable difference" level for 6-MM. Storage of carrots at 1 degree C was suitable to maintain low levels of 6-MM for a period of at least 17 weeks. No effect of Alternaria spp. infection was observed on 6-MM occurrence. The fate of 6-MM during carrot juice processing was also investigated by using different enzyme formulations for maceration and blanching procedures. Levels of 6-MM in blanched carrots obtained by boiling water or steam treatment were reduced by 69 or 33%, respectively, as compared to fresh carrots. No decrease in 6-MM levels was observed after maceration with pectinolytic enzyme preparations (Rapidase Carrot Juice and Ultrazym AFP-L). A reduction of 6-MM by 85 or 94% was obtained after the entire cycle of carrot juice processing, depending on the blanching procedure used. 相似文献
47.
Effects of land use and landscape on spatial distribution and morphological features of gullies in an agropastoral area in Sardinia (Italy) 总被引：3，自引：0，他引：3
According to the United Nations Convention to Combat Desertification (UNCCD), soil erosion is one of the main causes of desertification in the Northern Mediterranean. Recently, the role of channelled erosion in sediment delivery has been underlined by several studies. The aim of this paper is to describe a simplified experimental methodology settled up for studying the relationships between gully erosion and land use.The experimental site, which is wide about 720 ha, is located in a hilly area threatened by desertification in Central Eastern Sardinia (Italy). This area is characterised by irregular morphology with steep slopes, where agropastoral activities, particularly tillage with heavy machinery and overgrazing, are causing severe soil erosion and compromising agricultural productivity. Previous studies carried out in the same area highlighted the need to quantify and monitor soil erosion in order to outline management guidelines for farmers. In this study, an integrated methodology that uses aerial photographs and field surveys was applied to inventory the gullies of the area. The degree of activity and morphological characteristics of gullies were assessed in the field by measuring the position and shape of gully heads, and their width and depth. Total gully volume was also estimated. Relationships among distribution, density and morphological characteristics of gullies, and geomorphological features, land use and land use historical dynamics of the area have been studied. The results show the very significant impact of agricultural activities on gully activity and density. 相似文献
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The evaluation criteria applied on a 34 set of minor and main olive varieties of a restricted and climatically disfavoured region of Italy have allowed to clearly identify some local varieties with valuable agro-ecological adaptation and outstanding oil quality traits. These traits are necessary for the establishment and/or improvement of olive culture in a disadvantaged cultivation area. The molecular and morphological analyses have allowed to establish the identity of local unknown ecotypes, establishing their relationships either with other local varieties or with wide-spread cultivars, while the evaluation of oil quality parameters ranked their suitability for further exploitation. 相似文献
50.
Effect of aromatable androgen (17‐methyltestosterone) on induced maturation of silver European eels (Anguilla Anguilla): Oocyte performance and synchronization 
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下载免费PDF全文 Oliviero Mordenti Pietro Emmanuele Antonio Casalini Pieter Mark Lokman Annalisa Zaccaroni Andrea Di Biase Albamaria Parmeggiani 《Aquaculture Research》2018,49(1):442-448
The reproductive performances of silver European eel in term of gonad development and egg production, employing slow‐release implants with the androgen 17‐MT (1 mg) in combination with traditional weekly injection of carp pituitary extract (CPE) was evaluated. Wild female European eels (Anguilla anguilla) underwent a standard induction protocol with CPE and were randomly divided into three groups (N‐group, no implant; Y‐group, with implant; and control, C‐group, no treatment). The results showed that 17‐MT‐treated females (Y‐group) reproduced spontaneously about 6 weeks earlier than the N‐group females with a saving of almost 40% in CPE and time of induction. Concerning artificial induction of maturation in female silver eels, our study demonstrated that they positively respond to androgen exposure also in terms of eggs productivity. Indeed, Y‐group was more productive than N‐group: in Y‐group, 11 eels ensured an eggs production that exceeded 50% of initial body weight (BW), whereas in N‐group only three eels have exceeded this value. The results suggest that 17‐MT should be considered in future protocols for the improvement of the artificial reproduction of female silver European eels. 相似文献