Sampling design and its effect on population monitoring: How much monitoring do turtles really need?     

Andrew L. Jackson  Annette C. Broderick  Fiona Glen  Brendan J. Godley 《Biological conservation》2008,141(12):2932-2941

Marine turtles are a taxon of world-wide conservation concern. Effective long-term monitoring is hampered by the fact that populations are widely dispersed except during the breeding season. Thus most monitoring programmes focus on nesting beaches, necessitating resource intensive studies, often over months, that could conceivably, be less comprehensive and focus on more parsimonious sampling. We analyse 11 years of exhaustive monitoring data for two species of Mediterranean marine turtles (Chelonia mydas and Caretta caretta). We resample using a variety of plausible sub-sampling regimens to estimate the total annual nesting population. We project our dataset into the future applying a range of population change rates to explore how adopting a monitoring programme based on sub-sampling would, for example, affect our ability to detect population decline. We show that accurate annual population estimates can be achieved with as few as 14 days of survey effort providing monitoring spans the peak of nesting. Furthermore, the modelled impact of sampling-based monitoring suggests that the duration to detect population change is not increased greatly. Our findings have implications for all marine turtle monitoring and have applicability to other animal groups. It is often considered desirable to perform exhaustive monitoring, with aversion of basing policy recommendations on partial data. However, comprehensive long-term monitoring programmes, particularly in developing nations, although presenting a number of advantages, are often impossible. Accurate total annual censuses can be achieved through a variety of sub-sampling regimens without sacrificing the ability to detect changes in the population trends over time. In this example, a solid block of at least 3 weeks sampling that encompasses the peak of the nesting season is advised.  相似文献   

Body condition scoring (BCS) in corn snakes (Pantherophis guttatus) and comparison to pre-existing body condition index (BCI) for snakes     

Angela Gimmel  Sabine Öfner  Annette Liesegang 《Journal of animal physiology and animal nutrition》2021,105(Z2):24-28

In the veterinary profession, the body condition score (BCS) plays an important role in the assessment of patients. It is a subjective, tactile method of evaluating body fat and muscle mass and is used in numerous species. Recognizing obesity (or the contrary, emaciation) is important for veterinarians treating reptiles and could be facilitated by a BCS. An existing form of body condition assessment already used is the body condition index (BCI), where the residuals from a regression of body mass on body length are calculated. Therefore, the goal of this study was to provide practitioners with a BCS system for corn snakes (Pantherophis guttatus) and to test it against the BCI. A total of 22 corn snakes (Pantherophis guttatus), stationed at the “Auffangstation für Reptilien” in Munich (reptile rescue centre, RRC), were subject of this study. Each had the following measurements taken: body weight (BW), snout–tail tip length (STL), snout–vent length (SVL) and circumference in the middle (C). Manual palpation of spine, area between vertebral spinous and transverse process, ribs and neck of each snake was performed by three veterinarians and assigned to specific scores by each examiner. A BCS (mean of examiners’ scores) was given to each snake according to manual palpation. The BCS system was chosen to be out of 5 in 0.5-point steps with 2.5 considered as ideal BCS. In the studied snakes, the BCS ranged from 1.5 to 3.5, with a median of 2.5. The median BW was 309 g (75–967 g), the median STL was 123 cm (79–153 cm), the median SVL was 104 cm (73–133 cm) and the median C was 7.5 cm (4.3–11 cm). BCS and BCI were positively correlated. A BCS includes a manual palpation of the animal and thus gives the examiner additional information to the objectively measured/calculated index.  相似文献   

Ripening reduces the shrinkage of processed dredged material     

Oing  Katja  Gröngröft  Alexander  Eschenbach  Annette 《Journal of Soils and Sediments》2020,20(1):571-583

Purpose

The utilization of dredged material in dike construction as a substitute for traditionally used materials is considered as an option to preserve natural resources such as marsh sediments. As a prerequisite for this application, the equivalency with respect to soil physical and mechanical properties of the materials must be assessed. Previous investigations have shown pronounced differences in shrinkage behavior and desiccation cracking between sediments and dredged material. The key objective of the study was to assess whether shrinkage of processed dredged material can be reduced by further processing, i.e., dewatering, which can be referred to as ripening.

Materials and methods

To compare the shrinkage behavior of the materials, three different methods of different scales were applied. Small-scale methods conducted were the standard procedure for the determination of the shrinkage limit and the determination of the coefficient of linear extensibility (COLE_rod). Large-scale shrink-swell experiments were carried out in a specially constructed test system with 90 l capacity for a period of up to 385 days. Here the materials were ripened, i.e., air-dried, until shrinkage almost ceased, and a rewetting-air-drying cycle was conducted. Shrinkage and swelling were determined during the processes by measuring the changes in volume. On the ripened materials, COLE_rod was determined.

Results and discussion

The experiments show that the shrinkage behavior of processed dredged material can be ameliorated by ripening. COLE_rod of the ripened materials were about 20–80% lower than COLE_rod of the un-ripened materials. The large-scale shrink-swell experiments showed that shrinkage in the second drying cycle amounted to less volume than in the first drying cycle and that shrinkage behavior in contrast to the first drying cycle, where pronounced proportional shrinkage was observed, was dominated by structural and residual shrinkage in this cycle.

Conclusions

Ripening of processed dredged material is considered a useful pre-treatment option to ameliorate the shrink-swell behavior of processed dredged material and to obtain a better functional equivalency with traditionally used dike construction materials such as fine-grained marsh sediments.

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Association of Treponema spp. with canine periodontitis     

Nordhoff M  Rühe B  Kellermeier C  Moter A  Schmitz R  Brunnberg L  Wieler LH 《Veterinary microbiology》2008,127(3-4):334-342

To evaluate the association of oral Treponema (T.) spp. with severity of canine periodontitis, subgingival plaque samples of dogs of various breeds undergoing surgery were investigated. A wide range of oral Treponema spp. was analysed by a molecular and culture-independent approach applying DNA-DNA dot blot hybridization analysis and fluorescence in situ hybridization using Treponema specific oligonucleotide probes specific for phylogenetic groups I-VII of oral treponemes as well as probes specific for T. socranskii and T. denticola. To assess the periodontal status of affected dogs clinical parameters were measured and the periodontal status was classified from grade 0 (physiological periodont) to 3 (severe periodontitis). The periodontal status correlated significantly with an increasing concentration of volatile sulfur compounds (VSC, r=0.854) determined with a Halimeter, indicating a positive correlation between the presence of VSC-producing bacteria and periodontitis. In this study Treponema spp. of phylogenetic groups III, V-VII were not detected in any sample, whereas T. denticola-like treponemes were found only in 2 of 51 animals. However, treponemes belonging to phylogenetic groups I, II and IV of oral treponemes or T. socranskii were found in up to 64.84% of the dogs. The detection rate of Treponema spp. was significantly associated with an increased periodontal status. Treponemes present in periodontal lesions were also visualized by fluorescence in situ hybridization of gingival biopsies showing Treponema spp. not only in the microbial biofilm but also within the gingival tissue. The data presented here indicate that oral Treponema spp. are associated with canine periodontitis. Similar to human periodontitis, treponemes of groups I, II and IV and T. socranskii were found more frequently the higher the degree of periodontitis was.  相似文献   

Evaluation of routine depopulation, cleaning, and disinfection procedures in the live bird markets, New York     

Trock SC  Gaeta M  Gonzalez A  Pederson JC  Senne DA 《Avian diseases》2008,52(1):160-162

During the past years surveillance for avian influenza has been conducted in the live bird markets (LBMs) in New York as well as other states along the east coast. Repeated attempts to eradicate H5 and H7 influenza from the New York markets have focused efforts on the LBMs themselves. Despite repeated mandatory market closures accompanied by cleaning and disinfecting (C/D) procedures, avian influenza virus continued to be isolated. In an effort to assess the adequacy of the C/D procedure, samples were collected in temporal proximity to the depopulation and C/D. Comparison of the pre-C/D (83% virus positive), at C/D approval (1.6% positive) and post-C/D testing (33% positive) indicate that the current procedures of C/D can be effective at eliminating these influenza viruses. However, reinfection via introduction of influenza-virus-positive birds can occur shortly after the market reopens.  相似文献   

Correlations between acute radiation scores and pain scores in canine radiation patients with cancer of the forelimb     

Carsten RE  Hellyer PW  Bachand AM  LaRue SM 《Veterinary anaesthesia and analgesia》2008,35(4):355-362

Objective To determine whether there is a correlation between skin acute radiation score (ARS) and pain scores and to determine if skin ARSs can be used to predict future pain scores and increased need for analgesia in dogs undergoing radiation therapy for cancer of the forelimb. Study design Prospective observational study. Animals Seven middle‐aged dogs of various breeds with cancer of the forelimb. Methods Each neoplasm was surgically removed and a histologic diagnosis was obtained. Curative intent radiation therapy was initiated 2½–4½ weeks after surgery. Curative intent radiation therapy was delivered as prescribed. Two trained observers scored the dogs using a visual analog pain scale (VAS), Glasgow composite measure of pain scale, short form (GCMPS) and skin ARS prior to each day’s therapy. Daily scores were averaged and scatter plots were developed. Generalized estimating equation regressions were used to calculate standard error, 95% confidence interval, and p‐values for each relationship. Confidence and prediction bands were plotted. Results A statistically significant correlation between skin ARS and VAS and GCMPS pain scores was identified indicating that as the skin ARS increased so did the pain scores. A general correlation between VAS and GCMPS scores was observed. Early (fraction days 1–6) GCMPS scores were significantly influenced by anxiety behavior unrelated to pain. Skin ARS was found to predict precisely current and future presence of pain, but could only predict a range of potential future pain scores based on the pain management approach in use during this study. Conclusions Skin ARS can provide valuable information for initiating preemptive analgesia and intensifying pain management during curative intent radiation therapy. Daily pain scoring with an acceptable pain scale should be used in conjunction with the skin ARS to improve patient pain management. Clinical relevance Pain is an anticipated consequence of curative intent radiation therapy. Understanding the correlation between pain and skin ARS may facilitate more effective pain management.  相似文献   

Correlation of plasma coagulation tests and fibrinogenClauss with rotational thromboelastometry parameters and prediction of bleeding in dogs     

Nathalie M. Enk  Annette P. N. Kutter  Claudia Kuemmerle‐Fraune  Nadja E. Sigrist 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2019,33(1):132-140

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Connectivity analysis as a tool for assessing restoration success     

Xenia K. Volk  Johannes P. Gattringer  Annette Otte  Sarah Harvolk-Schöning 《Landscape Ecology》2018,33(3):371-387

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Phosphorus availability,root exudates,and microbial activity in the rhizosphere   总被引：1，自引：0，他引：1  

Günther Schilling  Andreas Gransee  Annette Deuhel  Grit Leovi  Silke Ruppel 《植物养料与土壤学杂志》1998,161(4):465-478

Field and pot experiments showed that the P demand of wheat is highest in early stages of growth (up to 1.67 μg P per cm² root surface and day). The needed orthophosphate ions H₂PO₄? and HPO₄²-move from soil to the root by diffusion. This process is controlled by the concentration gradient of the diffusible phosphate and the effective diffusion coefficient according to Pick's first law. Root excretions (rhizodeposition) are able to affect both characteristics. The water soluble portion of rhizodeposition contains more than 50% of up to 8 different sugars, 10–40% carboxylic acids and 10–15 amino acids and amides. The composition varies in dependence on the age of the root parts and on nutrition (Zea mays L., Brassica napus L., Pisum sativum L.). Diffusion experiments using small soil blocks showed that 50–75% of the root exudates were decomposed by respiration within 3 days. The rest was largely chemically converted. Originally present sugars disappeared. Due to the biosynthesis of different organic acids from the individual sugars the mobilisation of Ca₃(PO₄)₂ by Pantoea agglomerans increased when the sugar mixture was derived from the rhizodeposition of P deficient plants with more pentoses instead of glucose and fructose (mainly effect of anions). In the rhizosphere therefore a mixture of rhizodeposition and its conversion products exists which affects the binding of phosphorus in soil and the P transport to the root. This should be considered both for the development of new soil extractants and for modelling the P supply to plants.  相似文献   

Reconstructing past ocean pH-depth profiles   总被引：1，自引：0，他引：1  

MR Palmer  PN Pearson  SJ Cobb 《Science (New York, N.Y.)》1998,282(5393):1468-1471

Measurement of boron isotope compositions in species of planktonic foraminifera that calcified their tests at different depths in the water column are used to reconstruct the pH profile of the upper water column of the tropical ocean. Results for five time windows from the middle Miocene to the late Pleistocene indicate pH-depth profiles similar to that of the modern ocean in this area, which suggests that this method may greatly aid in our understanding of the global carbon cycle.  相似文献