Shedding routes of Coxiella burnetii in dairy cows: implications for detection and control     

Guatteo R  Beaudeau F  Berri M  Rodolakis A  Joly A  Seegers H 《Veterinary research》2006,37(6):827-833

Reliable detection of Coxiella burnetii shedders is a critical point for the control of the spread of this bacterium among animals and from animals to humans. Coxiella burnetii is shed by ruminants mainly by birth products (placenta, birth fluids), but may also be shed by vaginal mucus, milk, and faeces, urine and semen. However, the informative value of these types of samples to identify shedders under field conditions is unknown. Our aim was then to describe the responses obtained using a real-time PCR technique applied to milk, vaginal mucus and faeces samples taken from 242 dairy cows in commercial dairy herds known to be naturally infected with Coxiella burnetii, and to assess their putative associations. Positive results were found in all types of tested samples even in faeces. No predominant shedding route was identified. Among the shedder cows, 65.4% were detected as shedders by only one route. By contrast, cows with positive results for all three samples were scarce (less than 7%). Testing a cow based on only one type of biological sample may lead to misclassify it with regards to its shedding of Coxiella burnetii and thereby underestimate the risk of bacterial spread within a herd.  相似文献   

Factors Responsible for the Co-occurrence of Forested and Unforested Rock Outcrops in the Boreal Forest     

Hugo Asselin  Annie Belleau  Yves Bergeron 《Landscape Ecology》2006,21(2):271-280

Rock outcrops in the boreal forest of Québec can show either of two different states: a forested state with >25% tree cover, and an unforested state (<25% tree cover). We tested three different hypotheses that might explain the co-occurrence of forested and unforested rock outcrops: (1) differences in bedrock geology, with unforested outcrops associated to bedrock types inimical to tree growth; (2) unforested outcrops as recently disturbed sites undergoing secondary succession towards a forested state; (3) unforested outcrops as an alternative stable state to forested outcrops, induced by post-fire regeneration failure. Digitized forest inventory maps were used along with bedrock geology maps and time-since-fire maps to compare forested and unforested outcrops for bedrock geology type and date of the last fire. Field surveys were conducted on 28 outcrops (14 forested, 14 unforested) to gather information regarding tree species composition and site characteristics (thickness of the organic matter layer, percent cover of lichens, mosses and ericaceous shrubs). None of the three hypotheses explain the co-occurrence of forested and unforested rock outcrops in the boreal forest of Québec. Both outcrop types occur on the same bedrock geology types. Unforested outcrops are not recently disturbed sites in early-successional states, as no clear distinction could be made in tree species composition and date of the last fire between the two outcrop types. Forested and unforested outcrops are not alternative stable states, as unforested outcrops are unstable and cannot maintain themselves through time in the prolonged absence of fire. Hence, unforested rock outcrops could be viewed as degraded, diverging post-fire types maintained by the late Holocene disturbance regime, characterized by high fire frequencies.  相似文献   

No transmission of Potato spindle tuber viroid shown in experiments with thrips (Frankliniella occidentalis, Thrips tabaci), honey bees (Apis mellifera) and bumblebees (Bombus terrestris)     

Steen Lykke Nielsen  Annie Enkegaard  Mogens Nicolaisen  Per Kryger  Mojca Vir??ek Marn  Irena Mavri? Ple?ko  Andreas Kahrer  Richard A. Gottsberger 《European journal of plant pathology / European Foundation for Plant Pathology》2012,133(3):505-509

Experiments were carried out to investigate whether Potato spindle tuber viroid (PSTVd) can be transmitted intra- and inter-species from infected Solanum jasminoides to non-infected S. jasminoides and S. esculentum and from infected Brugmansia sp. to S. esculentum by Frankliniella occidentalis and Thrips tabaci by leaf sucking. The F. occidentalis experiments also included feeding on pollen prior to feeding on PSTVd-infected leaf. No thrips-mediated transmission of PSTVd was recorded. The possibility of PSTVd transmission by Apis mellifera and Bombus terrestris during their feeding/pollinating activities within ornamentals and from ornamentals to S. esculentum was included, and no bee-mediated transmission was recorded.  相似文献   

The Epidemiology of Mountain Bike Park Injuries at the Whistler Bike Park,British Columbia (BC), Canada     

Zachary Ashwell  Mary Pat McKay  Jeffery R. Brubacher  Annie Gareau 《The Forestry Chronicle》2012,23(2):140-145

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Potential Noncutaneous Sites of Chelonid Herpesvirus 5 Persistence and Shedding in Green Sea Turtles Chelonia mydas     

Annie Page-Karjian  Nicole L. Gottdenker  Jordyn Whitfield  Lawrence Herbst  Terry M. Norton  Branson Ritchie 《Journal of aquatic animal health》2013,25(3):136-142

Abstract

Chelonid herpesvirus 5 (ChHV5), the likely etiologic agent of sea turtle fibropapillomatosis (FP), is predicted to be unevenly distributed within an infected turtle, in which productive virus replication and virion shedding occurs in cutaneous tumor keratinocytes. In this study, we measured and compared ChHV5 DNA quantities in tumors, skin, urine, major organs, and nervous tissue samples from green turtles Chelonia mydas. These samples were taken from the carcasses of 10 juvenile green turtles with and without clinical signs of FP that stranded in Florida during 2014. Quantitative PCR for ChHV5 UL30 was used to identify ChHV5 DNA in tumors, skin, heart, kidney, nerves, and urine sampled from five out of five FP-positive and three out of five FP-free turtles. The most frequently co-occurring sites were cutaneous tumor and kidney (n = 4). Novel data presented here include the identification of ChHV5 DNA in kidney, heart, and nerve samples from three FP-free turtles. These data support candidate nontumored anatomic sites of ChHV5 DNA localization and mobilization during two different disease states that may be involved in the ChHV5 infection cycle.

Received September 8, 2016; accepted April 17, 2017 Published online July 26, 2017  相似文献   

Sulfated beta-glucan derived from oat bran with potent anti-HIV activity     

Wang SC  Bligh SW  Zhu CL  Shi SS  Wang ZT  Hu ZB  Crowder J  Branford-White C  Vella C 《Journal of agricultural and food chemistry》2008,56(8):2624-2629

China is a major producer of oats; the annual harvested area of 350,000 ha yields approximately 465,000 tons, giving an average yield of 1.33 tons/ha. The bran is not used for animal feed as it is of poor digestibility and low nutritive content and is considered a waste byproduct. Therefore, it is advantageous to produce a value-added product from the bran. We extracted the native polysaccharide, a linear (1-3)-, (1-4)-linked beta-glucan (OBG) from the oat bran and synthesized a sulfated derivative OBGS containing 36.5% sulfate. OBGS had potent activity against a primary isolate of human immunodeficiency virus (HIV-1) in peripheral blood mononuclear cells at a concentration (EC(50)=5.98 x 10(-4) microM) approximately 15,000 times below its cytotoxic concentration. OBGS was also active postinfection (EC(50)=5.3 x 10(-4) microM) and protected pretreated peripheral mononuclear cells (EC(50)=5.2 x 10(-2) microM) washed free of the compounds prior to infection. Thus, OBGS has potential as a vaginal microbicide and is the first such report for oat bran derived sulfated beta-glucan.  相似文献   

Diagnostic utility of clinical and laboratory test parameters for differentiating between sudden acquired retinal degeneration syndrome and pituitary‐dependent hyperadrenocorticism in dogs     

Annie Oh  Melanie L. Foster  Jonathan G. Williams  Chaowen Zheng  Hongyu Ru  Katharine F. Lunn  Freya M. Mowat 《Veterinary ophthalmology》2019,22(6):842-858

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Surgical decompression,with or without adjunctive therapy,for palliative treatment of primary vertebral osteosarcoma in dogs     

Alexandra Dixon  Annie Chen  John H. Rossmeisl  Beverly Sturges  Karen Vernau  Jonathan M. Levine  Arturo Otamendi  Peter Early  Alix Partnow  Lara Curtis  Stephanie Thomovsky  Rebecca A. Packer  Daniela A. Mauler 《Veterinary and comparative oncology》2019,17(4):472-478

Vertebral osteosarcoma (OSA) is the most common primary vertebral tumor in dogs, however studies examining the survival time after surgical decompression of these tumors are limited. There is also limited information regarding the benefit of adjunctive treatments such as radiation therapy or chemotherapy in these patients. The goal of this study was to determine survival time of dogs with primary vertebral OSA after palliative decompressive surgery alone and combined with radiation therapy and/or chemotherapy. Records from 22 client‐owned dogs diagnosed with primary vertebral OSA and treated with decompressive surgery were collected retrospectively from eight referral institutions. Survival time was assessed for dogs treated with surgery alone as well as dogs who received adjunctive radiation therapy and/or chemotherapy. Median survival time in the 12 dogs treated with surgery alone was 42 days (range: 3‐1333 days). The three dogs treated with surgery and chemotherapy had a median survival time of 82 days (range: 56‐305 days). Only one dog was treated with surgery and radiation therapy; this dog survived 101 days. Six dogs were treated with surgery, radiation therapy and chemotherapy; these dogs had a median survival time of 261 days (range: 223‐653 days). Cause of death in all cases that survived the initial postoperative period was euthanasia secondary to confirmed or suspected tumor regrowth. The results of this study suggest that definitive radiation therapy, possibly combined with concurrent chemotherapy, significantly improves survival in dogs treated with palliative decompressive surgery for vertebral OSA and should be the treatment of choice in selected cases.  相似文献   

Usage patterns of carbapenem antimicrobials in dogs and cats at a veterinary tertiary care hospital     

Alison Smith  Annie S. Wayne  Claire L. Fellman  Marieke H. Rosenbaum 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2019,33(4):1677-1685

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Circulating neurohormone imbalances in canine sudden acquired retinal degeneration syndrome and canine pituitary‐dependent hypercortisolism     

Annie Oh  Melanie L. Foster  Katharine F. Lunn  Freya M. Mowat 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2019,33(6):2587-2594

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