Pathogenicity and molecular phylogenetic analysis reveal a distinct position of the banana fingertip rot pathogen among the Burkholderia cenocepacia genomovars     

M. Ansari  S. M. Taghavi  S. Zarei  K. Miri  P. Portier  E. Osdaghi 《Plant pathology》2019,68(4):804-815

Banana (Musa spp.) is one of the most widely cultivated subtropical fruits around the globe. Banana cultivation has been extensively increased in southeastern Iran over the last two decades. Recently, banana fruits possessing rotten and blackened fingertip symptoms were observed in Sistan-Baluchestan, Iran. Isolation and characterization of the causal agent showed that the pathogen belongs to the multifaceted bacterial species Burkholderia cenocepacia. Pathogenicity tests and host range assays showed that the strains were pathogenic on banana, as well as carrot, onion and potato. All the strains were resistant to 50 mg L⁻¹ rifampicin and 200 mg L⁻¹ copper sulphate. Phylogenetic analysis of 16S rRNA and recA gene sequences showed that the strains belong to two different genomovars of B. cenocepacia (III-A and III-B), which also include environmental and cystic fibrosis associated strains of the species. The results obtained from recA phylogeny were confirmed using multilocus sequence analysis (MLSA), although MLSA showed that the banana strains were clustered as a novel phylogroup among the members of both genomovars. Banana-pathogenic B. cenocepacia strains isolated in Iran were different from the strains isolated in Taiwan, as the ‘B. cepacia epidemic strain marker’ reported in the Taiwanese strains was absent from Iranian strains. To the authors’ knowledge, this is the first MLSA-based study on the banana-pathogenic strains of B. cenocepacia. However, further in-depth molecular studies are needed to decipher the relationships between the banana fingertip rot pathogen and the clinical strains of B. cenocepacia.  相似文献   

都市型薄皮甜瓜品种比较试验     

张静  哈雪姣  贾文红  乔媛媛  郝敬虹  李婷 《蔬菜》2021,(4):69-72

为筛选出适宜在北京种植的优质都市型薄皮甜瓜品种,选取8个品种的薄皮甜瓜为试验材料,对比分析其田间长势及果实品质。结果表明:8个参试品种田间长势及果实品质均有显著差异,其中梨形瓜中丘比特果实大,外观好,果肉最厚,心糖最高,综合果实品质最优秀;长筒形瓜中博洋9号果实外观及品质较优秀,单果质量最大,果形指数好,心糖最高。综合看来,丘比特和博洋9号这2个品种可在北京地区进一步推广种植。  相似文献   

富含硒钴锌的苜蓿青干草在牛羊饲喂中的效果     

李明  哈斯  介晓磊  郭孝  李建平  蒋士传 《家畜生态》2011,(4):34-37

通过在荷斯坦奶牛和杜泊羊日粮中添加5%高硒型、高硒钴型、高硒钴锌苜蓿青干草,研究3种苜蓿青干草对牛羊饲料转化和生产特点的影响。结果表明,功能型苜蓿青干草能够显著提高牛羊的饲料转化率,其中高硒钴和高硒钴锌苜蓿青干草有利于提高羊和牛的日增重,而高硒型青干草有利于提高荷斯坦奶牛的产奶量。  相似文献   

鸡公山自然保护区森林植被生物量及活碳蓄积量研究   总被引：3，自引：0，他引：3  

严茂超  杨柳春  李海涛  胡聃  张照喜  杜化堂  哈德龙 《河南林业科技》2004,24(4):1-4,6

运用鸡公山科学考察资料及1999年森林资源清查资料,采用森林材积源生物量推算方法研究了鸡公山森林植被生物量及其活碳蓄积量。结果表明:鸡公山森林植被生物量总值为309 202t,平均森林植被生物量为111.7t／hm2,高于全国平均水平(77.4t／hm2);鸡公山森林植被的总活碳蓄积量为154 601t,平均活碳密度为56MgC／hm2,高于中国森林植被活碳密度的平均水平(38.7 MgC／hm2),但低于全球平均碳密度(86MgC／hm2)。不同林型活碳蓄积密度分析结果表明,马尾松、杉木和栎类林木的活碳蓄积密度分别为30MgC／hm2,39 MgC／hm2和70MgC／hm2,均高于全国同类型森林植物的活碳蓄积密度的平均值,而次生阔叶混交林的碳蓄积密度略低于全国平均水平。鸡公山自然保护区67％的森林为中龄林,27％的森林为幼龄林,在增加碳蓄积方面还有巨大的潜力。  相似文献   

Stockplant Management for Optimized Rhizogenesis in Tectona grandis Stem Cuttings     

Sanjay Singh  A. S. Bhandari  S. A. Ansari 《New Forests》2006,31(1):91-96

A 5-year stand of teak (Tectona grandis L. f.) was coppiced in 1999 and converted into a vegetative multiplication garden. Subsequently, three harvesting regimes for the collection of single node stem cuttings were imposed: (1) once – in March (H₁), (2) twice – in March and September (H₂) and (3) three times in March, July and November (H₃). Cuttings were treated basally with either:- T₀ – control (6 h in water), T₁ – half the recommended dose of a mixture of IBA and thiamine (500 ppm IBA + 400 ppm thiamine) or T₂ – the full dose of the same mixture (1000 ppm IBA + 800 ppm thiamine). Cuttings receiving IBA + thiamine rooted significantly better than untreated cuttings, but even the best treatment only resulted in 38.3 ± 3.8% rooting. This treatment produced the greatest number of roots (5.2–12.1). The full dose treatment appears to have been supra-optimal. Rooting ability was also affected by the frequency of stockplant pruning, with cuttings from stockplants pruned twice per year having the greatest rooting percentage (27.8 ± 3.8%) and the most roots (9.2 ± 4.8). This bi-annual pruning (H₂) resulted in the greatest number of rooted propagules (2.6 and 4.2 times more than H₁ and H₃, respectively). There was a significant interaction between Treatment × Pruning frequency. Bi-annual hedging of teak stockplants is recommended for practical purposes, although further work is required to achieve commercially acceptable levels of rooting from coppiced tree stumps.  相似文献   

Frequency Hydraulic Aperture about Fracture Seepage in Rock Mass     

XU Guang xiang  HA Qiu-ling  ZHANG Yong xing 《保鲜与加工》2001,(5):50-55

The frequency aperture given in this paper is a new method that describes the fracture aperture composition. The aperture curve plays a role in describing the aperture distribution and uniformity. In particular, the frequency hydraulic aperture is of great importance in seepage analysis. It overcomes limitations of hydraulic aperture and mean aperture. Based on existing information, the result of example calculation shows that the hydraulic aperture e h equals to the median aperture e 50 . This paper also obtains that the frequency hydraulic aperture e sh equals to e 48 through finite element numerical modeling for various type of random fracture.  相似文献   

Connect Technique of Laminated Object Manufacturing Using Metallic Materials as Modeling Materials     

YI Shu ping  HA Jin  LIN Li hong  ZHANG Jin 《保鲜与加工》2002,(2):1

To solve some key problems existed in the manufacturing of metallic functional part via Laminated Object Manufacturing (LOM), a new technique using vacuum solid-state pressure diffusion weld technique to connect the metallic slice sheets is presented in this paper. The following conclusions can be draw from the experiments introduced specially: the size in the stack direction shrink at the rate of less than 1% and the shrinking is owed to regularly error, the atom-diffusion between two combined interface occurred clearly and new crystallites shaped meanwhile, shearing intension of well connected diffusion section is more than 100 MPa and micro hardness of well-knit portion is the same to matrix's. It can be proved from the result above that the vacuum solid-state pressure diffusion technique weld is a perfect technique for connecting metallic slice sheets used in the manufacturing of metallic functional part via LOM.  相似文献   

糖水龙葵果罐头制作工艺研究     

韩天龙  金明晓 《保鲜与加工》2006,6(2):33-34

通过单因素多水平、多因素多水平的正交实验对龙葵果实罐藏工艺进行研究,结果表明,加工条件为烫漂温度60℃,烫漂时间10min,糖水浓度60%,排气温度40℃,排气时间15min.在此条件下罐头产品感官与品质最佳.  相似文献   

动物溶菌酶基因的研究进展   总被引：1，自引：0，他引：1  

孙国庆  哈福  苗永旺  李大林 《中国牛业科学》2012,38(3):51-54,59

溶菌酶[Lysozyme(EC3.2.1.17),LYZ]是机体先天免疫系统中一个重要的效应分子,参与机体多种免疫反应,在溶菌过程中形成一个水解体系,破坏和消除侵入动物体内的病原,从而实现机体的免疫防御。它可以有效抑制葡萄球菌、大肠杆菌等一系列病原菌,是重要的奶牛乳房炎抗性因子之一。本文综述了溶菌酶基因的研究进展,包括其结构与进化、变异及应用前景等。  相似文献   

Arginine Levels Affect Growth and CSN3 Gene Expression of Dairy Cows Mammary Epithelial Cells in Vitro     

XU Bolin  WANG Mengzhi  ZHANG Xingfu  HA Si  WANG Chunyan  AO Changjin  WANG Hongrong 《动物营养学报》2012,24(5)

本试验旨在研究培养基中添加不同浓度的精氨酸( Arg)对乳腺上皮细胞体外增殖及κ-酪蛋白(CSN3)基因表达的影响.选用中国荷斯坦奶牛乳腺上皮细胞进行体外培养,以无Arg的培养基(0.00 mg/L)为对照(0组),试验培养基分别添加69.50(0.25组)、139.00(0.50组)、278.00(1.00组)、556.00(2.00组)、1 112.00(4.00组)和2 224.00 mg/L(8.00组)的精氨酸.结果表明:Arg能促进乳腺上皮细胞的增殖,24 h时,0.25～4.00组与0组相比均差异显著(P＜0.05),8.00组与0组相比差异不显著(P＞0.05);48和72 h时,各试验组与0组相比均差异显著(P＜0.05).Arg能促进CSN3基因的表达,各试验组与0组相比均差异显著(P＜0.05),当Arg浓度为556.00 mg/L时,CSN3基因表达量最高.结果提示,Arg对乳腺上皮细胞增殖及CSN3基因表达均具有明显的促进作用,且在Arg浓度为69.50～1112.00 mg/L时促细胞增殖效果较佳,在Arg浓度为556.00 mg/L时促CSN3基因表达作用最强.  相似文献