辐解产物2-十二烷基环丁酮的测定方法   总被引：2，自引：0，他引：2  

张海伟  哈益明  王锋  林若泰 《中国农业科学》2009,42(3):989-995

 【目的】建立1种含脂辐照食品中特异性辐解产物2－十二烷基环丁酮的萃取分离以及定量分析的新方法。【方法】采用硅胶层析柱萃取分离、气相色谱－质谱联用检测,对淋洗剂浓度、硅胶活化程度、收集时间及气相色谱-质谱条件等技术参数进行优化。【结果】该方法对2－十二烷基环丁酮标准品的最低检出限为0.005 mg?L-1,0.1～2.0 mg?L-1的添加回收率为75.9%～103.9%,相对标准偏差（RSD）均小于5.7%。【结论】与欧盟标准方法EN1785进行比较,硅胶柱分离法具有成本低、提取效率高、重现性好、检出限低、承载样品量大等优点。  相似文献   

有机肥防治马铃薯早疫病试验   总被引：2，自引：0，他引：2  

张建平  程玉臣  哈斯 《马铃薯杂志》2012,(5):291-294

2010-2011年通过田间小区方法进行了有机肥防治马铃薯早疫病（Alternaria solani）试验。结果表明：对于品种‘克新1号’和‘费乌瑞它’667 m^2施有机肥1 500-5 000 kg及667 m^2施有机肥1 500 kg＋复合化肥50 kg均对马铃薯早疫病有显著的防治作用,防效为38.8%-68.8%,特别是667 m^2施有机肥5 000 kg、667 m^2施有机肥1 500 kg＋复合化肥50 kg防效更加显著,增产21.2%-27.8%。667 m^2施有机肥1 500-5 000 kg及施有机肥1 500 kg＋复合化肥50 kg比施复合化肥50 kg提早出苗1-4 d不等,品种‘费乌瑞它’提早出苗更明显,但施用或增施有机肥对2个品种出苗率无影响。施用有机肥为马铃薯早疫病的防治提供一个新的途径。  相似文献   

减蛋综合征病毒环介导等温扩增检测方法的建立   总被引：1，自引：0，他引：1  

董春娜  李刚  李伟  张坤  史利军  哈小琴  胡永浩 《甘肃农业大学学报》2011,46(2):1-5

根据GenBank登录的减蛋综合征病毒(EDSV)六邻体基因的保守区,设计了3对环介导等温基因扩增(LAMP)引物,优化反应体系,并对其灵敏性、特异性进行验证.结果表明:LAMP优化后的反应体系为外引物浓度0.1μm01·L-1,内引物浓度0.8 μmol·L-1,环引物浓度0.4μmol·L-1,Mg2+浓度8.0 ...  相似文献   

Toxic effects of neem-based insecticides on Pieris brassicae (Linn.)     

Fazil Hasan  M. Shafiq Ansari 《Crop Protection》2011,30(4):502-507

Four neem-based insecticides, Neemix^® (0.25% EC @ 20 mg azadirachtin/liter), Ecozin^® (3% EC @ 20 mg azadirachtin/liter), Agroneem^® (0.15% EC @ 4.8 mg azadirachtin/liter) and Neem oil (0.25% EC azadirachtin @ 20 mg azadirachtin/liter) and a non-commercial neem leaf powder, were evaluated for oviposition deterrence, antifeedant effect to larvae and toxicity to eggs and larvae of Pieris brassicae (Linn.) on cabbage leaves in the laboratory. The concentrations tested were with in the ranges of recommended field rates. Oviposition deterrence in no-choice, two-choice and six-choice assays, was observed for all the treatments. They exhibited significant (P < 0.01) oviposition deterrence on P. brassicae when compared with a non-treated control. Cabbage leaves treated with the neem-based insecticides were used as an egg-laying substrate. Numbers of eggs oviposited by P. brassicae adults on treated cabbage leaves were significantly lower then those treated with water, but no significant differences were detected among the neem insecticides. They also deterred feeding by Pieris larvae and exhibited significant antifeedant effects. Larvae of P. brassicae on treated leaves stopped feeding and dropped from the leaf, resulting in no or minimal damage. Direct contact with neem-based insecticides decreased the survival of eggs. Survival of larvae fed for 9 days on leaves treated with neem-based insecticides was reduced to 51%, 49%, 48%, 24% and 18% in the Neem oil, Neemix, Agroneem, Ecozin and neem leaf powder treatments, respectively. Therefore, it can be concluded from the present investigations that neem-based insecticides had oviposition deterrence, antifeedant and toxic effect to P. brassicae.  相似文献   

Effects of different brassicaceous host plants on the fitness of Pieris brassicae (L.)     

Fazil Hasan  M. Shafiq Ansari 《Crop Protection》2011,30(7):854-862

Pieris brassicae (L.) is a destructive cosmopolitan pest of cruciferous crops. It is present wherever its host plants occur and it is considered to be one of the most widely distributed of all the Lepidoptera. We investigated the affect of various host plants on the fitness of P. brassicae to survive, develop and reproduce. We quantified development time, survival and mortality on cabbage (Brassica oleracea capitata), cauliflower (Brassica oleracea botrytis), radish (Raphanus sativus), broccoli (Brassica oleracea italica), and mustard (Brassica campestris) under laboratory conditions. The developmental time from eggs to adult eclosion was the shortest (30.3 days) on cabbage and the longest (38.0 days) on radish. However, adult longevity was significantly (P < 0.001) greatest on cabbage compared with the other host plants tested. Similarly, the developmental rate of P. brassicae was found to be faster on cabbage than other cole crops. Moreover, the lowest immature mortality was evident on cabbage and highest on mustard and radish. Differences in reproductive parameters of the P. brassicae among host plants were analyzed using pseudo-values, which were produced by jackknife re-sampling. The data showed that the higher intrinsic rate of natural increase (0.116 females/female/day) was found on cabbage. Cabbage was recognized as the most suitable host for P. brassicae because of shorter developmental period, higher percentage survival, lower doubling time (6.00), and higher number of adult emergence (29.7%).  相似文献   

Effects of different levels of sanguinarine on antioxidant indices,immunological responses,ileal microbial counts and jejunal morphology of laying hens fed diets with different levels of crude protein          下载免费PDF全文

M. Bavarsadi  A. H. Mahdavi  S. Ansari‐Mahyari  E. Jahanian 《Journal of animal physiology and animal nutrition》2017,101(5):936-948

This study was carried out to assess the effects of different levels of sanguinarine on antioxidant indices, immunological responses, serum biochemical parameters, ileal microbial counts and jejunal morphology of laying hens fed on diets with different levels of crude protein (CP). A total of 180 laying hens were subjected into nine dietary treatments with four cages of five birds each. Experimental treatments consisted of three levels of CP (85.0, 92.5 and 100% of Hy‐Line W36 manual recommendation) and three levels of sanguinarine (0.00, 3.75 and 7.50 mg/kg) as a 3 × 3 factorial arrangement of laying hens which fed during a 70‐day feeding trial. The in vitro study showed that sanguinarine exhibited sevenfold and threefold decreased antioxidant activities to inhibit 2‐2‐diphenyl‐1‐picric hydrazyl free radical as well as ferric ion reducing rather than butylated hydroxyl toluene. Although using the decremental levels of CP caused the increase in heterophil‐to‐lymphocyte ratio (p < 0.01), dietary administration of sanguinarine could suppress the serum cholesterol and malondialdehyde concentrations as well as heterophil‐to‐lymphocyte ratio (p < 0.05). Additionally, decreasing CP content resulted in the decreased percentage of albumin (p < 0.05); however, it had no negative effects on humoral immunity. Nonetheless, feeding of at least 3.75 mg/kg sanguinarine led to the remarkable increases in serum gamma globulin concentration (p < 0.01) and secondary (p < 0.05) antibody titres against sheep red blood cells. Moreover, a decline in dietary CP content led to higher villi height and crypt depth (p < 0.05; p < 0.001) and consequently decreased villi height‐to‐crypt depth ratio (p < 0.001) than the optimum level (100% CP). In spite of the effects of sanguinarine on the suppression of Escherichia coli and Salmonella counts (p < 0.05), it markedly enhanced villi height‐to‐crypt depth ratio as well as lamina propria lymphatic follicles extent, simultaneously (p < 0.001). Therefore, in spite of the detrimental effects of feeding low‐CP diets on lymphocytes and serum albumin percentages, and villi height‐to‐crypt depth ratio, the administration of incremental levels of sanguinarine could improve cellular and humoral immunity, decrease ileal microbial counts and in turn improve the intestinal health indices in laying hens.  相似文献   

国家湿地公园生态文化景观营造研究——以银川阅海国家湿地公园建设为例     

冯玉璞  哈梅娟 《现代农业科技》2011,(24):266-267

阐述了在银川阅海国家湿地公园规划、建设、管理过程中,对国家级湿地公园保护和利用及与地域生态文化景观特色结合的实践认识,对加快我国国家级湿地公园的建设与管理步伐有一定的借鉴意义。  相似文献   

细胞凋亡信号传导通路的研究进展   总被引：3，自引：2，他引：1  

王力俭  田可川  吴伟伟  徐新明  张廷虎  张艳花  哈尼克孜 《中国畜牧兽医》2011,38(10):132-135

细胞凋亡是多细胞生物调控生物体的发育、细胞更新和维持内环境稳定的一种重要机制。目前发现细胞凋亡存在死亡受体通路、线粒体通路和内质网通路3条通路,且各通路之间相互联系,共同促进细胞凋亡。作者综述了近年来细胞凋亡信号传导通路及其调控的研究进展。  相似文献   

大肠杆菌不耐热肠毒素B亚单位在大肠杆菌中的表达及其特性研究     

葛俊伟  姜艳平  杨敏  哈卓  乔薪瑗  唐丽杰  李一经 《东北农业大学学报》2010,41(6)

将大肠杆菌不耐热性肠毒素B亚单位(LTB)基因eltb克隆到表达载体pET-28a中,转化大肠杆菌BL21/DE3/plyss。采用SDS-PAGE和Western blot分析,结果表明,在大肠杆菌中高效表达了LTB蛋白,其产量约为细菌总蛋白的20%,GM1-ELISA方法鉴定目的重组蛋白可与GM1结合,具有潜在的佐剂活性;由纯化的重组蛋白免疫小鼠制备的抗血清效价为1:16000,并且可以特异性识别天然毒素。结果表明,所表达的LTB有良好的抗原性和佐剂活性,制备的抗血清具有良好的免疫活性,为下一步以LTB作为佐剂的黏膜疫苗研究提供基础材料。  相似文献   

A comparative study on the rumen microbial populations,hydrolytic enzyme activities and dry matter degradability between different species of ruminant     

Yea Hwang MOON  Ji Un OK  Shin Ja LEE  Jong Kyu HA  Sung Sill LEE 《Animal Science Journal》2010,81(6):642-647

A comparative study among Korean native cow (Hanwoo), Holstein dairy cow, Korean native goat and crossbred sheep on the population and marker concentration of ruminal microbes, the activities of carboxymethylcellulase (CMCase), xylanase and amylase, and in situ dry matter (DM) degradability were conducted. Twelve ruminally cannulated animals, three of each species, were used. Animals were fed the same diet containing 40% formula feed and 60% rice straw at the level of 2.5% of body weight. Total viable microbial populations in the rumen fluid were significantly (P < 0.01) greater for bacteria and fungi in goat than those of Holstein. The protozoan population among ruminant species was the reverse from that of bacteria. The concentrations of 2,6‐diaminopimelic acid and chitin as markers for bacteria and fungi in the rumen fluid, respectively, were highest in goat, which is in accordance with the above population data. The concentration of aminoethylphosphonic acid as marker of protozoa was highest in Hanwoo and lowest in sheep (P < 0.01). Goat had the highest (P < 0.01) activities of all the enzymes investigated among ruminants. In situ effective degradation of the DM of rice straw was approximately 19% higher in the rumen of goat compared with other animals.  相似文献