Chromosomal Analysis of Cases with Chimerism that Consisted of Normal Cells and Cells with 1/29 Translocation in Homosexual Twin Cows and their Calves     

Y-I Miyake  M Kanemaki  A Chiba 《Reproduction in domestic animals》1997,32(3):175-177

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Trientine, a copper-chelating agent, induced apoptosis in murine fibrosarcoma cells in vivo and in vitro     

Hayashi M  Nishiya H  Chiba T  Endoh D  Kon Y  Okui T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(2):137-142

Anti-copper treatments have been investigated to determine whether they suppress angiogenesis and tumor development since Cu is widely accepted as being required for angiogenesis. We examined the effects of treatment with trientine, a copper-chelating agent, on tumor development in a murine xenograft model using fibrosarcoma-derived transplantable QRsp-11 cells and C57BL/6 mice and induction of apoptosis in tumor cells and endothelial cells in vivo and in vitro. The tumor volumes increased more slowly in trientine-treated mice than in untreated mice. Tumor volumes in the treated mice were significantly smaller than those in the untreated mice at 24 days postinoculation (d.p.i.) of tumor cells. A cluster of pyknotic tumor cells and morphological abnormalities in capillary endothelial cells were observed in the tumors of trientine-treated mice but not in the tumors of untreated mice. The proportions of apoptotic and necrotic cells in the tumors of treated mice were approximately 3.5-fold higher than those in the tumors of untreated mice at 14 d.p.i. When the cells were treated with trientine in vitro, mouse endothelial cells and bovine primary endothelial cells showed an approximately 10-fold higher sensitivity to trientine than QRsp-11 cells in terms of D37. However, the proportion of apoptotic cells in endothelial cells was significantly lower than that in QRsp-11 cells after treatment with trientine. These results show that apoptosis was induced in tumor cells by treatment with trientine in vivo and in vitro.  相似文献   

Cesium radioactivity in peripheral blood is linearly correlated to that in skeletal muscle: Analyses of cattle within the evacuation zone of the Fukushima Daiichi Nuclear Power Plant          下载免费PDF全文

Tomokazu Fukuda  Yasushi Kino  Yasuyuki Abe  Hideaki Yamashiro  Jin Kobayashi  Yoshinaka Shimizu  Atsushi Takahashi  Toshihiko Suzuki  Mirei Chiba  Shintaro Takahashi  Kazuya Inoue  Yoshikazu Kuwahara  Motoko Morimoto  Hisashi Shinoda  Masahiro Hiji  Tsutomu Sekine  Manabu Fukumoto  Emiko Isogai 《Animal Science Journal》2015,86(1):120-124

The accident at the Fukushima Daiichi Nuclear Power Plant (FNPP) released a large amount of radioactive substances into the environment. Furthermore, beef contaminated with radioactive cesium above the 500 Bq/kg safety standard was circulated in the food chain in 2011. Japanese consumers remain concerned about the safety of radioactively contaminated food. In our previous study, we detected a linear correlation between radioactive cesium (¹³⁷Cs) activity in blood and muscle around 500 to 2500 Bq/kg in cattle. However, it was unclear whether the correlation was maintained at a lower radioactivity close to the current safety standard of 100 Bq/kg. In this study, we evaluated 17 cattle in the FNPP evacuation zone that had a ¹³⁷Cs blood level less than 10 Bq/kg. The results showed a linear correlation between blood ¹³⁷Cs and muscle ¹³⁷Cs (Y = 28.0X, R² = 0.590) at low radioactivity concentration, indicating that cesium radioactivity in the muscle can be estimated from blood radioactivity. This technique would be useful in detecting high‐risk cattle before they enter the market, and will contribute to food safety.  相似文献   

Electrical manipulation of magnetization reversal in a ferromagnetic semiconductor     

Chiba D  Yamanouchi M  Matsukura F  Ohno H 《Science (New York, N.Y.)》2003,301(5635):943-945

We report electrical manipulation of magnetization processes in a ferromagnetic semiconductor, in which low-density carriers are responsible for the ferromagnetic interaction. The coercive force HC at which magnetization reversal occurs can be manipulated by modifying the carrier density through application of electric fields in a gated structure. Electrically assisted magnetization reversal, as well as electrical demagnetization, has been demonstrated through the effect. This electrical manipulation offers a functionality not previously accessible in magnetic materials and may become useful for reversing magnetization of nanoscale bits for ultrahigh-density information storage.  相似文献   

Gentian Kobu-sho-associated virus: a tentative, novel double-stranded RNA virus that is relevant to gentian Kobu-sho syndrome     

Kappei Kobayashi  Go Atsumi  Yasuya Iwadate  Reiko Tomita  Ken-ichi Chiba  Shiho Akasaka  Masahiro Nishihara  Hideyuki Takahashi  Naoto Yamaoka  Masamichi Nishiguchi  Ken-Taro Sekine 《Journal of General Plant Pathology》2013,79(1):56-63

A virus-like dsRNA of about 23 kbp was detected in gentian plants showing Kobu-sho syndrome including stunting, shortened internodes, and tumors on stems, nodes and roots. Nucleotide sequence analysis has suggested that this dsRNA is related to Pestivirus species but not to any other plant viruses. It was protected from externally added RNase, suggesting that the dsRNA is encapsidated. The dsRNA was co-extracted in a crude homogenate of glutaraldehyde-fixed tissue with the virus-like particles that have been associated previously with Kobu-sho syndrome in gentian (Usugi et al. Jpn J Phytopathol 76:21–24, 2010). The RNA sequence was detected in more than 99 % of Kobu-sho gentian individuals but in less than 20 % of apparently healthy gentian individuals from fields affected with Kobu-sho. Thus, we propose naming the virus Gentian Kobu-sho-associated virus.  相似文献   

Speciation and monitoring test for inorganic arsenic in white rice flour     

Narukawa T  Hioki A  Chiba K 《Journal of agricultural and food chemistry》2012,60(4):1122-1127

A monitoring test for arsenic species in white rice flour was developed and applied to flours made from 20 samples of polished rice collected from locations all over Japan. The arsenic species in white rice flour made from five samples each of four types of rice were analyzed by HPLC-ICP-MS after a heat-assisted aqueous extraction. The total arsenic and major and minor element concentrations in the white rice flours were measured by ICP-MS and ICP-OES after microwave-assisted digestion. 91 ± 1% of the arsenic in the flours was extractable. Concentrations of arsenite [As(III)], arsenate [As(V)], and dimethylarsinic acid (DMAA) were closely positively correlated with the total arsenic concentrations. The total arsenic concentration in flours made from rice collected around Japan was 0.15 ± 0.07 mg kg(-1) (highest, 0.32 mg kg(-1)), which is very low. It was thus confirmed that the white rice flour samples collected in this experiment were not suffered from noticeable As contamination.  相似文献   

Isolation and characterization of a phenolic antioxidant from the Pacific oyster (Crassostrea gigas)     

Watanabe M  Fuda H  Jin S  Sakurai T  Ohkawa F  Hui SP  Takeda S  Watanabe T  Koike T  Chiba H 《Journal of agricultural and food chemistry》2012,60(3):830-835

Using an oxygen radical absorbance capacity (ORAC) assay, antioxidant activity was detected in the ethanol extract of the Pacific oyster, which was purified by sequential extraction with organic solvents. The ethyl acetate fraction showed the strongest antioxidant activity and was further purified, yielding a single compound [as assessed by thin-layer chromatography (TLC) and reverse-phase high-performance liquid chromatography (HPLC)]. This compound was identified as 3,5-dihydroxy-4-methoxybenzyl alcohol on the basis of (1)H and (13)C nuclear magnetic resonance (NMR), heteronuclear multiple-bond correlation (HMBC), and electrospray ionization-mass spectrometry (ESI-MS) spectral analyses, a conclusion that was confirmed by chemical synthesis. The concentration of the compound was 6.7 mg/100 g of whole oyster meat wet weight. This amphiphilic antioxidant retarded the copper-mediated oxidation of low-density lipoproteins (LDLs) and the generation of thiobarbituric acid reactive substances. Furthermore, the compound showed substantial antioxidant activity using the ORAC and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays compared to natural antioxidants. Although the same compound was previously found in brown algae, its presence in other organisms and antioxidant activity are reported here for the first time.  相似文献   

Modeling of thermoregulation in the skunk cabbage in relation to meteorological factors     

Ken Takahashi  Takanori Ito  Shigeki Chiba  Kikukatsu Ito  Hiroshi Osada 《Agricultural and Forest Meteorology》2009,149(6-7):1032-1036

A model is proposed to calculate the spadix temperature of the skunk cabbage Symplocarpus foetidus which regulates its internal temperature at around 20 °C during the female stage even when the ambient air temperature drops below freezing. The model is based on energy balance of the spadix under field conditions. The input data are standard meteorological data and spadix parameters. The model output was compared with experimental data collected under different climate conditions. The agreement between observed and calculated spadix temperatures was fairly good.  相似文献   

Rhipicephalus (Boophilus) microplus embryo proteins as target for tick vaccine     

Seixas A  Oliveira P  Termignoni C  Logullo C  Masuda A  da Silva Vaz I 《Veterinary immunology and immunopathology》2012,148(1-2):149-156

Rhipicephalus (Boophilus) microplus is one of the most widely distributed tick in the world. The control of the parasite is based mainly on the use of chemical acaricides, which are produced from a limited set of molecules. These drugs induce selection of acaricide-resistant ticks, and are an important source of environmental pollution. An approach based on anti-tick vaccines may circumvent these obstacles. Characterization of the physiological function of tick molecules may be useful to develop new vaccines. Previously, we reported the ability of some tick proteins as inducers of protective immune response. Vaccination studies using tick proteins like native (nBYC), recombinant (rBYC) egg-yolk aspartic endopeptidase and cysteine endopeptidase (VTDCE) from R. microplus and glutathione S-transferase (Hl-GST) from Haemaphysalis longicornis demonstrated the immunogenicity and antigenicity of these proteins in bovines. Eventually, immunization with these proteins triggered a partial immune response against R. microplus infestation in cattle, manifested mainly as a reduction in egg fertility (7.7% and 13.9% for nBYC, 5.9% for rBYC; 4.7% for VTDCE, 7.9% for Hl-GST), and in the number of fully engorged ticks (18.2% for rBYC, 14.6% for VTDCE, 53% for Hl-GST). The data so far obtained suggest that these proteins have potential to be used as antigens in an anti-tick vaccine. Other proteins involved in tick embryogenesis also have this potential, like THAP and BmCl1, which are enzymes with key roles in vitellin and hemoglobin hydrolysis. Moreover, the identification of analogous proteins present in other tick species may bring information about the way to develop a vaccine against multiple tick species which can help to solve the problem faced by numerous countries where animals are parasitized by more than one tick species. The aim of the present review is to comprehensibly summarize the data obtained in the last few years by our collaborative research, discussing the efforts we have made to find antigens efficient enough for a cattle tick-controlling vaccine. This review discusses tick physiology studies aimed at the selection of possible targets, characterization of the selected proteins with emphasis on their biochemical and immunological aspects and results of vaccine trials on bovines.  相似文献   

Recent races of spinach downy mildew pathogen <Emphasis Type="Italic">Peronospora farinosa</Emphasis> f. sp. <Emphasis Type="Italic">spinaciae</Emphasis> in Japan     

Masaharu Kubota  Osamu Tamura  Yasuhiro Nomura  Noriko Orihara  Norihito Yamauchi  Kengo Yonemoto  Kenichiro Chiba  Nobutaka Someya 《Journal of General Plant Pathology》2017,83(2):117-120

Isolates of spinach downy mildew fungus, Peronospora farinosa f. sp. spinaciae, collected at seven sites in Japan from December 2013 to March 2016, were identified as belonging to races 8, 10, 12 and 13, and one was unauthorized. Races 10, 12, 13 and the unauthorized were first found in Japan.  相似文献