Probiotic efficiency of Bacillus sp. in Labeo rohita challenged by Aeromonas hydrophila: assessment of stress profile,haemato‐biochemical parameters and immune responses          下载免费PDF全文

Ankita Nandi  Goutam Banerjee  Suhas Kumar Dan  Koushik Ghosh  Arun Kumar Ray 《Aquaculture Research》2017,48(8):4334-4345

Fish are always susceptible to a wide variety of deadly pathogens which cause a huge loss in aquaculture industries. In this investigation, we have demonstrated the in vivo probiotic efficiency of Bacillus sp. MVF1 (GenBank Acc. No. KP256503) in Labeo rohita challenged with pathogenic strain of Aeromonas hydrophila (MTCC 1739). To check the probiotic potential of the selected bacterial strain, fish were divided into four groups: control, D1, D2 and D3. A total of 100 days (70 days probiotic feeding + 71th day sampling and 28 days challenged test + 29th day sampling) of feeding trial was conducted. To establish the probiotic potential of Bacillus sp. MVF1, certain haematological parameters (haemoglobin, total erythrocyte and leucocyte count), serum biochemical parameters (total protein, albumin and globulin), immune parameters (serum lysozyme and total IgM levels) and hepatic stress profile (malondialdehyde production, superoxide dismutase and catalase activity) have been measured. Our results demonstrated that red blood cell number, white blood cell number and haemoglobin content were much higher in D2 group fish compared to other groups and control fish. Similarly, total protein contents, albumin concentration, globulin concentration, lysozyme activity and IgM production were also recorded to be highest in D2 group fish. This finding clearly indicated the probiotic potential of Bacillus sp. MVF1 in L. rohita. Furthermore, our results also demonstrated that 1 × 10⁷ CFU g^?1 feed (D2) provides better immunity compared to 1 × 10⁵ (D1) and 1 × 10⁹ (D3). Due to beneficial effects, the bacterium Bacillus sp. MVF1 might be useful in aquaculture industries to reduce the disease susceptibility.  相似文献   

Effect of biofilm on water quality and growth of <Emphasis Type="Italic">Etroplus suratensis</Emphasis> (Bloch, 1790)     

M. T. Shilta  Narinder Kumar Chadha  P. K. Pandey  Paramita Banerjee Sawant 《Aquaculture International》2016,24(2):661-674

An experiment was conducted to compare the efficiency of biofilm production in natural and artificial substrates and to study their effect on water quality and growth of Etroplus suratensis. Four different substrates were used for biofilm formation: paddy straw (T1), sugarcane bagasse (T2), polyvinylchloride (PVC) pipe (T3) and plastic sheet (T4). The experiment was carried out in mud-bottomed fibre-reinforced plastic tanks (300 L) in triplicates. About 3000 cm² surface area (600 g) of each substrate was suspended in water supplemented with fertilizers. Only cow dung and urea were applied in control tanks. The tanks were stocked with 25 fishes with average weight of 9.1 ± 0.22 g. The overall mean value of heterotrophic bacteria in substrate was found higher in straw followed by bagasse, plastic and PVC. The dominant genera of bacteria in the substrate were Bacillus, Pseudomonas and Micrococcus in that order of preponderance. The mean phytoplankton and zooplankton density on the substrates were higher in bagasse followed by straw, plastic and PVC. The biofilm developed on the substrate significantly reduced the ammonia nitrogen and nitrite-nitrogen content of water. The growth of fishes was significantly (P < 0.05) higher in substrate-based treatments than that in the control with better results in bagasse followed by straw, plastic and PVC. The conclusions of the present study are that biofilm produced on natural substrates, especially on bagasse, enhanced growth of E. suratensis and reduced the necessity of water exchange during the culture, which certainly decreases the cost of Etroplus production.  相似文献   

Development of Gossypium hirsutum lines with cytoplasmic bollworm tolerance from Gossypium arboreum and Gossypium tomentosum     

S. L. Ahuja  S. K. Banerjee  J. Singh  P. Singh  V. V. Singh  D. Monga  O. P. Tuteja 《Plant Breeding》2009,128(6):712-715

American cotton [Gossypium hirsutum (L.)] grown in India belongs to the race Latifolium. It is prone to bollworms [Helicoverpa armigera (Hubner), Erias vitella (Fabricius), Pectinophora gossypiella (Saunders)] infestation which causes considerable crop loss. Two breeding lines ‘Bikaneri (BN)‐arboreum (ARB)‐16’ and ‘BN‐tomentosum (TOM)‐277’ are cytoplasmic diverse genetic stocks. They were developed by crossing experimental lines ‘Delta Branch Experiment Station (DES)‐ARB16’ and ‘DES‐TOM277’ (non‐recurrent parents) with G. hirsutum‘BN’ (recurrent parent). Compared with BN these stocks possessed higher amount of gossypol, flavonol and phenol contents in leaves, stem and square. Plants were tolerant to bollworms and inherited comparable agronomic properties (yield, boll weight, plant height, number of monopods and sympods and fibre quality). Cotton breeders can use these lines for breeding cotton resistant to bollworm.  相似文献   

Molecular controls of tuberization     

David J. Hannapel  Hao Chen  Faye M. Rosin  Anjan K. Banerjee  Peter J. Davies 《American Journal of Potato Research》2004,81(4):263-274

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Comparison of four antibiotics with indigenous marine Bacillus spp. in controlling pathogenic bacteria from shrimp and Artemia     

Banerjee S  Devaraja TN  Shariff M  Yusoff FM 《Journal of fish diseases》2007,30(7):383-389

Use of antibiotics for the control of bacterial diseases in shrimp culture has caused several adverse impacts to the industry. This has resulted in the search for alternative environment friendly approaches to overcome bacterial infections. This study was conducted to investigate the use of beneficial bacteria as an alternative to antibiotics. Ten pathogenic bacterial species isolated from shrimp, Penaeus monodon, and Artemia cysts were tested for susceptibility to indigenous marine Bacillus subtilis AB65, Bacillus pumilus AB58, Bacillus licheniformis AB69 and compared with oxytetracycline, chloramphenicol, gentamicin and bacitracin, which are common antibiotics used in Asian aquaculture. The Bacillus spp. were isolated from the local marine environment for bioremediation use in shrimp hatcheries and were proven to reduce total ammonium nitrogen. The pathogenic bacterial isolates were 90% susceptible to B. subtilis AB65, 70% susceptible to B. pumilus AB58 and B. licheniformis AB69 and 100% susceptible to oxytetracycline, chloramphenicol and gentamicin but only 40% to bacitracin. Two representative isolates of the vibrio group, Vibrio alginolyticus VaM11 and Vibrio parahaemolyticus VpM1, when tested for competitive exclusion by a common broth method using the marine Bacillus spp., showed decreased viable counts from 10(8) to 10(2) cfu mL(-1). The results suggest that the action of the marine bacteria appears to be significant in protecting the host shrimp against pathogenic bacteria. In addition to the alternative use of antibiotics, the selected marine bacteria had additional bioremediation properties of reducing ammonia.  相似文献   

Identification and Pathogenicity of Pseudomonas aeruginosa DJ1990 on Tail and Fin Rot Disease in Spotted Snakehead          下载免费PDF全文

Dibya J. Saikia  Pritam Chattopadhyay  Goutam Banerjee  Bandita Talukdar  Dandadhar Sarma 《Journal of the World Aquaculture Society》2018,49(4):703-714

Tail and fin rot disease (TFRD) is a big issue in the production of spotted snakehead, Channa punctata Bloch. The aims of the present study were to isolate and identify the bacterial pathogen causing TFRD, to detect histopathological changes in tissues (fin, tail, liver, and kidney), and to ascertain the antibiotic sensitivity pattern of the isolate. Out of six bacterial isolates, only the isolate DJ1990 was found to be the causal candidate of TFRD in C. punctata. Identical histopathological changes were detected in tail, fin, liver, and kidney under light and scanning electron microscope in both collected diseased fish and artificially infected fish. The isolate was identified as Pseudomonas aeruginosa strain DJ1990 (National Center for Biotechnology Information Ace. No. KX709967) based on the biochemical characterization tests and 16S rDNA sequence‐based phylogeny analysis. Artificial challenge test demonstrated that the strain DJ1990 was highly virulent (100% mortality at 48 h of postinjection period at the concentration of 1.5 × 10⁷ CFU/g of body weight) for C. punctata. The isolate exhibited sensitivity to the broad‐spectrum antibiotics but was resistant against aztreonum. To the best of our knowledge, this is the first report of P. aeruginosa as a TFRD‐causing candidate in C. punctata.  相似文献   

Oxidative DNA damage preventive activity and antioxidant potential of Stevia rebaudiana (Bertoni) Bertoni, a natural sweetener     

Ghanta S  Banerjee A  Poddar A  Chattopadhyay S 《Journal of agricultural and food chemistry》2007,55(26):10962-10967

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Narrow sense heritability estimates of bacterial leaf spot resistance in pseudo F2 (F1) population of mulberry (Morus spp.)     

Rita Banerjee  N. K. Das  S. G. Doss  A. K. Saha  A. K. Bajpai  B. B. Bindroo 《European journal of plant pathology / European Foundation for Plant Pathology》2012,133(3):537-544

Bacterial leaf spot incited by Xanthomonas campestris pv. mori is a devastating foliar disease of mulberry reported globally. Host plant resistance is the most sustainable and economic control measure but so far unexplored. Highly heterozygous plant behaviour and scant genetic information of bacterial leaf spot resistance limits a targetted breeding approach in mulberry. In the present research eight pseudo-F₂(F₁)full-sib progenies derived from selected resistant and susceptible sources were evaluated symptomatically for bacterial leaf spot resistance under natural disease occurrence in 2008 and 2009. Significant variation for bacterial leaf spot resistance was observed in the parents and progenies. Broad sense heritability estimate (0.9) indicates that selection of resistant genotypes can be useful for exploitation in future advanced breeding programs for mulberry. High narrow sense heritability estimates (0.76)[2008] and (0.79)[2009] suggest additive gene effects for the disease resistant trait. The continuous frequency distribution of diseases severity across the progenies indicates that bacterial leaf spot resistance in mulberry may be inherited quantitatively.  相似文献   

Action of the fungicide tridemorph on the glucose,lactate and succinate dehydrogenase activities of some tridemorph-sensitive and -resistant bacteria     

Abul Kalam  Ajit K. Banerjee 《Pest management science》1995,43(1):41-45

The systemic morpholine fungicide tridemorph, which is known to exert its antifungal action through inhibition of ergosterol biosynthesis, can also inhibit the growth of organisms which are incapable of sterol biosynthesis. It was found to inhibit strongly glucose and lactate dehydrogenase activities in cultures of four Gram( +) bacteria, Rhodococcus sp. AK 1, Bacillus cereus Frankland & Frankland, Bacillus subtilis (Ehrenberg) Cohn, Nocardia asteroides and a Gram(?) bacterium, Rhizobium leguminosarum. Growth of these bacteria was inhibited by tridemorph at concentrations between 7 and 60 mg litre^?1. In contrast, similar dehydrogenase activities in other Gram(?) organisms, Escherichia coli Cast. & Chalm. and Azotobacter vinelandii, which showed no growth inhibition at 200 mg litre ^?1 tridemorph, were either not inhibited or inhibited only slightly. Similarly, succinate dehydrogenase activity in Rhodococcus sp. AK 1 was more strongly inhibited by tridemorph than that in E. coli. In cell-free extracts of Rhodococcus sp. AK1 and E. coli, lactate dehydrogenase activity was also inhibited by tridemorph to a much greater extent in the sensitive strain (63%) than in the resistant ones (8%).  相似文献   

Prevalence of latent cases of Babesia equi infection in some parts of North West India as measured by the capillary agglutination test     

D V Malhotra  D P Banerjee  O P Gautam 《Equine veterinary journal》1978,10(1):24-26

The prevalence of Babesia equi infection in north west India was assessed by means of the capillary tube agglutination (CA) test. The particulate antigen used in the test was potent and no cross reaction with other related haemaprotozoa was observed. The serological survey showed that from 323 horses from 3 localities there was an overall incidence of 50.1 per cent. In Haryana the incidence was 38.3 per cent in the 196 horses tested, in Uttar Pradesh it was 47.2 per cent from 72 animals and in Rajasthan it was 96.4 per cent from 55 horses.  相似文献