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11.
Nagamine MK Sanches DS Pinello KC Torres LN Mennecier G Latorre AO Fukumasu H Dagli ML 《Veterinary research communications》2011,35(6):391-399
Mast cell tumor (MCT) is one of the most prevalent neoplasms that affect skin and soft tissue in dogs. Because mast cell tumors
present a great variety of clinical appearance and behavior, their treatment becomes a challenge. Trichostatin A (TSA), an
antifungal antibiotic, has shown inhibitory effects on the proliferation and induction of apoptosis in various types of cancer
cells. In order to evaluate the potential of trichostatin A as a therapeutic drug, cells of grade 3 MCT were cultured and
treated with concentrations of 1 nM to 400 nM of TSA. MTT assay and trypan blue exclusion assays were performed to estimate
cell growth and cell viability, and cell cycle analysis was evaluated. TSA treatment showed a reduction in numbers of viable
cells and an increase of cell death by apoptosis. The cell cycle analysis showed an increase of hypodiploid cells and a reduction
of G0/G1 and G2/M –phases. According to these results, trichostatin A may be an interesting potential chemotherapeutic agent
for the treatment of canine MCT. 相似文献
12.
Thaís Mendes Sanches Cavalero Lorenzo Garrido Teixeira M. Segabinazzi Verônica Flores da Cunha Scheeren Camila P. Freitas-Dell'Aqua Frederico Ozanam Papa 《Reproduction in domestic animals》2020,55(12):1808-1811
The present study compared the quality of sperm collected by artificial vagina or pharmacologically induced ejaculation from a 10-year-old thoroughbred stallion with seminal vesiculitis. The pharmacological protocol involved intravenous administration of detomidine (0.01 mg/kg) and oxytocin (20 IU) and successfully induced ejaculation in all attempts of semen collection. Sperm motility, plasma membrane and acrosome integrity (PMAI), reactive oxygen species (ROS) levels, polymorphonuclear neutrophil (PMN) percentage, and bacterial profiles of fresh and cooled semen (5°C for 24 hr) were evaluated. Semen obtained by the pharmacological method presented reduced seminal volume, decreased PMN percentage and superior sperm motility in cooled samples. Moreover, higher PMAI and lower ROS levels were observed in semen collected by the pharmacological method. Therefore, pharmacologically induced ejaculation is an alternative to obtain semen with minimal contamination and with sperm of superior quality and longevity from stallions with seminal vesiculitis. 相似文献
13.
Renato Beozzo Bassanezi Luiz Henrique Montesino Eduardo Sanches Stuchi 《European journal of plant pathology / European Foundation for Plant Pathology》2009,125(4):565-572
Citrus huanglongbing (HLB), associated with Candidatus Liberibacter asiaticus and Ca. L. americanus and transmitted by the Asian psyllid Diaphorina citri, is the most serious disease of citrus worldwide because of crop devastation and difficulty to control. Since 2004, approximately 3 million trees were eliminated in attempts to limit its spread in Brazil. Where HLB becomes endemic, the disease progression in the orchard and the increasing symptom severity throughout the tree canopy can be relatively fast, greatly reducing the economic life of affected orchards because of tree decline and yield reduction. Although the majority of the fruit from symptomatic branches drop before harvest, a significant amount of affected fruit remain attached, are available for harvest, and can affect juice quality. To quantify and compare the effects of HLB on fruit quality of the most important sweet orange cultivars grown in São Paulo State, 4–6 year-old sweet orange trees from 26 blocks (two of ‘Valencia Americana’, eight of ‘Hamlin’, four of ‘Westin’, seven of ‘Pera’, and five of ‘Valencia’) were selected prior to harvest. In each block, 14–21 HLB-symptomatic trees were chosen. In each tree, the quality of 20 fruit normal in appearance from asymptomatic branches and 20 symptomatic fruit from symptomatic branches were assessed. In general, compared to normal fruit, the symptomatic fruit were small, light, more acidic, and had lower juice percentage, Brix, total soluble solids per box, total soluble solids per fruit, and Brix/acidity ratio. These effects of fruit quality were less pronounced on early and mid season sweet orange cultivars than on late season cv. Valencia. 相似文献
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15.
Fabrício Packer Gonçalves Eduardo Sanches Stuchi Simone Rodrigues da Silva Eduardo Toller Reiff Lilian Amorim 《Scientia Horticulturae》2011
The production and commercialization of citrus seedlings inspected and produced in protected screenhouses has become mandatory in São Paulo State, Brazil since January 2003. This law was intended to avoid the dispersion of Citrus Variegated Chlorosis (CVC), disease caused by Xylella fastidiosa. Our objective was to compare the yield over 8 years of ‘Natal’ sweet orange trees grafted onto Rangpur lime obtained from healthy nursery plants and from plants artificially inoculated with X. fastidiosa. Yield was evaluated in an orchard planted in February 1999 with two treatments: (i) trees from healthy nursery plant, and (ii) trees from plants artificially inoculated with X. fastidiosa. The mean yield was 21% higher in trees from healthy nursery plants, as compared to trees from inoculated nursery plants. This difference represents a gain of approximately 203 boxes of 40.8 kg each, considering a planting density of 550 plants per hectare. 相似文献
16.
Roberto A R de Souza Wesley F da Annunciação Stephane M Lins Eduardo G Sanches Maurício L Martins Mônica Y Tsuzuki 《Aquaculture Research》2014,45(4):619-628
The interaction between cultured barber goby Elacatinus figaro (cleaner fish) and the dusky grouper Epinephelus marginatus, and the efficiency of the cleaner fish in removing ectoparasites were evaluated. When the interaction between these two species was observed, cleaner fish showed a preference for the largest groupers. In a second trial, treatments: TWC (Control) – two groupers without a cleaner fish, T1C – two groupers with one cleaner fish, T3C – two groupers with three cleaner fish and T6C – two groupers with six cleaner fish were tested in four replicates. After 8 days, monogeneans were removed and identified as Neobenedenia melleni. The highest mean abundance of parasites was found on the groupers in the TWC group (37 parasites per host) and the lowest on those in the T6C group (4.1 parasites per host). By increasing the number of cleaner fish, a higher cleaning efficiency was obtained, as observed in T6C, where almost 90% of the parasites were removed. Possibly, this removal would have been complete if the number of cleaners had not been reduced in the treatments due to the mortalities observed. This study demonstrates the possibility of using gobies to remove monogeneans and in improving grouper health. 相似文献
17.
Flávia Q. de Oliveira Arlindo L. Boiça Junior Maria de Lourdes Z. Costa Patricia A. Sanches Julio M. M. Walder Jacinto de L. Batista 《Phytoparasitica》2014,42(4):475-483
This study aimed to verify the preference and infestation level of Anastrepha fraterculus (Diptera: Tephritidae) [South American fruit fly] in fruits of guava cultivars and to correlate them to variables such as peel coloration, soluble solids and pH of fruit cultivars. The following cultivars were used: Pedro Sato, Paluma, Cascão and Século XXI. The infestation was evaluated in cages, considering two scenarios: no-choice and multiple choice. In both tests, evaluations of the fruit attraction to insects were conducted for a period of 1′, 3′, 5′, 10′, 20′, 30′, 1 h, 2 h, 6 h, 12 h and 24 h. The visit of A. fraterculus on the assayed cultivars in relation to the time was studied by logistic regression. After 10 days, the number of larvae in each fruit was recorded. In the multiple choice test, the visit proportions were significantly higher in the fruits of cvs. Século XXI and Pedro Sato than in those of cvs. Paluma and Cascão. In the no-choice test, the visit proportions were significantly lower in the Paluma fruits. In both tests, the rate of fruit infestation by A. fraterculus did not differ among cvs. Pedro Sato, Paluma and Cascão, whereas the fruits of cv. Século XXI were more infested. The indexes of pH did not interfere with the infestation of A. fraterculus, whereas a high rate of soluble solids and low color angle appear to be crucial for discriminating the fruits of the most susceptible cultivars. Infestation rate of Século XXI fruits displayed significant correlations with: °Brix (r= 0.7078) and color angle (h) (r= –0.9499) of guava fruits under the multiple choice conditions. 相似文献
18.
Effect of sward height on the fermentability coefficient and chemical composition of Guinea grass silage 
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下载免费PDF全文 P. K. Tomaz L. C. de Araujo L. A. Sanches S. N. dos Santos‐Araujo T. O. de Lima A. de A. Lino E. M. Ferreira 《Grass and Forage Science》2018,73(3):588-598
There is a high correlation between sward height and pasture sward structure. Therefore, in tropical grasslands, taking sward height into account has been a much better strategy in rotational stocking management than considering pre‐defined days of growth. Similarly, sward height could be used to determine the moment when tropical grasses present the best ensilability parameters. This study aimed to identify the sward height at which Panicum maximum cv. Mombaça (Guinea grass) provides the highest fermentability coefficient (FC) and to define the combination of additives that best improves the chemical composition of silage. Two trials were carried out in Selvíria, MS, Brazil, from 2015 to 2016. The first year was used to identify the highest FC, and the second year was used to identify the best combination of eight additives (citrus pulp [CIP], homofermentative and heterofermentative LAB, their combinations and control). Statistical analyses were performed using SAS (p < .05), and one contrast was defined as silage with CIP vs. silage without CIP. The height of 130 cm resulted in the highest FC (31.01). Silages inoculated with CIP had better quality than silages without CIP, due to the high crude protein (8.3 vs. 7.3% DM), DM recovery (98.6 vs. 93.3% DM), low pH (3.92 vs. 4.91) and NH3‐N values (2.49 vs. 14.73% total N). Sward height is a consistent parameter for determining the time of ensiling Guinea grass, and the inclusion of CIP is necessary to raise the silage quality. 相似文献
19.
J. W. Bossolani E. Lazarini F. L. Santos I. R. Sanches H. H. A. Meneghette L. F. Parra 《Communications in Soil Science and Plant Analysis》2018,49(15):1855-1868
The aim of this experiment was to study the lime and gypsum reapplied on surface and their impacts on maize crop under the sole and intercropped systems. The experiment is being developed in a dystrophic Oxisol, since 2011, and arranged in a randomized blocks design, in split-split plots, with four replications. As treatments in the plots, the maize was installed in two cropping systems: sole and intercropped with Urochloa. For the subplots, we used four doses of lime (0, 2000, 4000, and 6000 kg ha?1) and four doses of gypsum (0, 1500, 3000, and 4500 kg ha?1) distributed at random in the sub-subplots. The combination of intermediate doses of lime and gypsum resulted in higher foliar Ca content. The grain yield increased with the combination of lime and gypsum. The maize when cultivated in intercropped system is more productive, with a point of maximum for dolomitic lime of 3031 kg ha?1. 相似文献
20.
Using the same fish with different rules: A science‐based approach for improving management of recreational fisheries in a biodiversity hotspot of the Western South Atlantic 下载免费PDF全文
下载免费PDF全文 Domingos Garrone‐Neto Eduardo Antonio Sanches Felippe Alexandre Lisboa de Miranda Daros Cleber Mikio Rosa Imanobu Pietro Simon Moro 《Fisheries Management and Ecology》2018,25(4):253-260
In this study we tested the hypothesis that Centropomus parallelus Poey moves between areas where fishing tourism is important, but the management rules for recreational fisheries are different. Fieldwork was conducted from May 2014 to June 2017 in an estuary located in the border between the states of São Paulo and Paraná, South‐Southeastern Brazil. Through a mark and recapture experiment, 2.000 individuals were tagged, of which 42 were recaptured. Among these, six fish were recaptured in Paraná, after being tagged in São Paulo, and a fish was recaptured in São Paulo, after being tagged in Paraná. These results support the hypothesis tested and indicate an uneven use of C. parallelus by anglers in both states. Thus, the use of the species and the recreational fisheries regulations need to be reviewed in the study area, taking into account the ability of C. parallelus to move across political boundaries and the importance of a fish‐oriented fisheries management regime. 相似文献