Artificial insemination of gilts with bovine viral diarrhea virus-contaminated semen     

Gabriel Y. Storino  Marina L. Mechler-Dreibi  Eduarda B. Xavier  Artur S. Fioroto  Maria E. F. Oliveira  Edviges M. Pituco  Luis G. de Oliveira 《The Canadian veterinary journal. La revue veterinaire canadienne》2021,62(1):59

Bovine viral diarrhea virus (BVDV) is a pestivirus that infects swine and other species and has genetic and antigenic similarity to classical swine fever virus. The objective of this study was to mimic the infection of swine by contaminated semen and evaluate the effects on their reproductive tracts and litters. Six gilts were artificially inseminated with semen containing BVDV-2 ncp (LVB 16557/15) and 2 were inseminated with BVDV-free semen. Blood samples from all gilts were collected for polymerase chain reaction and virus neutralization tests. No viremia or neutralizing antibodies were detected, and all the litters were born healthy.  相似文献   

Transplanted hypothalamic neurons restore leptin signaling and ameliorate obesity in db/db mice     

Czupryn A  Zhou YD  Chen X  McNay D  Anderson MP  Flier JS  Macklis JD 《Science (New York, N.Y.)》2011,334(6059):1133-1137

Evolutionarily old and conserved homeostatic systems in the brain, including the hypothalamus, are organized into nuclear structures of heterogeneous and diverse neuron populations. To investigate whether such circuits can be functionally reconstituted by synaptic integration of similarly diverse populations of neurons, we generated physically chimeric hypothalami by microtransplanting small numbers of embryonic enhanced green fluorescent protein-expressing, leptin-responsive hypothalamic cells into hypothalami of postnatal leptin receptor-deficient (db/db) mice that develop morbid obesity. Donor neurons differentiated and integrated as four distinct hypothalamic neuron subtypes, formed functional excitatory and inhibitory synapses, partially restored leptin responsiveness, and ameliorated hyperglycemia and obesity in db/db mice. These experiments serve as a proof of concept that transplanted neurons can functionally reconstitute complex neuronal circuitry in the mammalian brain.  相似文献   

Three novel species of fungi associated with pine species showing needle blight-like disease symptoms     

Monteiro  Pedro  Gonçalves  Micael F. M.  Pinto  Glória  Silva  Beatriz  Martín-García  Jorge  Diez  Julio Javier  Alves  Artur 《European journal of plant pathology / European Foundation for Plant Pathology》2022,162(1):183-202

European Journal of Plant Pathology - Pine needle diseases, such as red band and brown spot needle blight, are serious pine diseases that threatens forests in many countries. Several outbreaks have...  相似文献   

High interferon type I responses in the lung, plasma and spleen during highly pathogenic H5N1 infection of chicken     

Moulin HR  Liniger M  Python S  Guzylack-Piriou L  Ocaña-Macchi M  Ruggli N  Summerfield A 《Veterinary research》2011,42(1):6

ABSTRACT: This study shows that high pathogenic H5N1 influenza virus infection of chicken induced high levels of bioactive interferon type I in the lung (4.3 × 105 U/mg tissue), plasma (1.1 × 105 U/mL), and spleen (9.1 × 105 U/mg tissue). In contrast, a low pathogenic attenuated H5N1 vaccine strain only induced approximately 24 times less IFN in the lung, 441 times less in the spleen and 649 less in the plasma. This was in the same range as a reassortant carrying the HA from the vaccine strain and the remaining genes from the high pathogenic virus. On the other hand, a reassortant virus with the HA from the high pathogenic H5N1 with the remaining genes from the vaccine strain had intermediate levels of IFN. The level of interferon responses related to the viral load, and those in the spleen and blood to the spread of virus to lymphoid tissue, as well as disease severity. In vitro, the viruses did not induce interferon in chicken embryonic fibroblasts, but high levels in splenocytes, with not clear relationship to pathogenicity and virulence. This, and the responses also with inactivated viruses imply the presence of plasmacytoid dendritic cell-like leukocytes within the chicken immune system, possibly responsible for the high interferon responses during H5N1 infection. Our data also indicate that the viral load as well as the cleavability of the HA enabling systemic spread of the virus are two major factors controlling systemic IFN responses in chicken.  相似文献   

The effect of photoperiod and feeding frequency on larval of the Amazonian ornamental fish Pyrrhulina brevis (Steindachner, 1876)          下载免费PDF全文

Galileu Crovatto Veras  Marcos Ferreira Brabo  Joel Artur Dias  Higo Andrade Abe  Zélia Maria Pimentel Nunes  Luis David Solis Murgas 《Aquaculture Research》2016,47(3):797-803

This study aimed to evaluate the performance of Pyrrhulina larvae, Pyrrhulina brevis, under different photoperiods and feeding frequencies. A completely randomized design was used, with four replicates in a 5 × 2 factorial, with five photoperiods (6L:18D, 12L:12D, 16L:8D, 20L:4D, 24L:0D) and two feeding frequencies (two or four times a day). Four hundred larvae with an initial length of 4.6 ± 0.09 mm were randomly distributed in 40, 1‐L containers at a density of 10 larvae/L. Feeding was performed with Artemia nauplii in the ratio of 150 Artemia nauplii/larva/feeding. After 15 days, all larvae were euthanized for measurements of length (mm), final weight (mg), survival rate (%) and larvae uniformity in weight an length (%). The data were subjected to analysis of variance and subsequently, in case of significance, a Tukey test was performed to compare means. There was no interaction between photoperiod and feeding frequency on studied variables (P > 0.05). Likewise, there was no influence of feed rate on the same variables (P > 0.05). There was only influence of photoperiod, where the larvae subjected to longest photoperiods (24L:0D and 20L:4D) showed improved weight and length (P < 0.05). Therefore, a photoperiod of 20L:4E, with feeding twice a day is recommended.  相似文献   

Control of fire blight (Erwinia amylovora) by a novel strain 49M of Pseudomonas graminis from the phyllosphere of apple (Malus spp.)     

Artur Mikiciński  Piotr Sobiczewski  Joanna Puławska  Robert Maciorowski 《European journal of plant pathology / European Foundation for Plant Pathology》2016,145(2):265-276

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Anthraquinones,Diphenyl Ethers,and Their Derivatives from the Culture of the Marine Sponge-Associated Fungus Neosartorya spinosa KUFA 1047     

Joana D. M. de S  Jos A. Pereira  Tida Dethoup  Honorina Cidade  Maria Emília Sousa  Inês C. Rodrigues  Paulo M. Costa  Sharad Mistry  Artur M. S. Silva  Anake Kijjoa 《Marine drugs》2021,19(8)

Previously unreported anthraquinone, acetylpenipurdin A (4), biphenyl ether, neospinosic acid (6), dibenzodioxepinone, and spinolactone (7) were isolated, together with (R)-6-hydroxymellein (1), penipurdin A (2), acetylquestinol (3), tenellic acid C (5), and vermixocin A (8) from the culture of a marine sponge-associated fungus Neosartorya spinosa KUFA1047. The structures of the previously unreported compounds were established based on an extensive analysis of 1D and 2D NMR spectra as well as HRMS data. The absolute configurations of the stereogenic centers of 5 and 7 were established unambiguously by comparing their calculated and experimental electronic circular dichroism (ECD) spectra. Compounds 2 and 5–8 were tested for their in vitro acetylcholinesterase and tyrosinase inhibitory activities as well as their antibacterial activity against Gram-positive and Gram-negative reference, and multidrug-resistant strains isolated from the environment. The tested compounds were also evaluated for their capacity to inhibit biofilm formation in the reference strains.  相似文献   

Regulatory T cells are decreased in acute RHDV lethal infection of adult rabbits     

Teixeira L  Marques RM  Aguas AP  Ferreira PG 《Veterinary immunology and immunopathology》2012,148(3-4):343-347

Rabbit hemorrhagic disease virus (RHDV) is the etiologic agent of rabbit hemorrhagic disease (RHD), an acute lethal infection that kills 90% of adult rabbits due to severe acute liver inflammation. Interestingly, young rabbits are naturally resistant to RHDV infection. Here, we have compared naturally occurring CD4(+)Foxp3(+) regulatory T cells (Tregs) between young and adult rabbits after infection by RHDV. The number and frequency of Tregs was decreased in the spleen of adult rabbits 24h after the RHDV infection; this was in contrast with the unchanged number and frequency of splenic Tregs found in young rabbits after the same infection. Also, serum levels of IL-10 and TGF-β were enhanced in the infected adult rabbits whereas no alteration was observed in infected young rabbits. However, this increase is accompanied by a burst of pro-inflammatory cytokines, but seems not able to prevent the death of the animals with severe acute liver inflammation in few days after infection. Since Tregs downregulate inflammation, we conclude that their decrease may contribute to the natural susceptibility of adult rabbits to RHDV infection.  相似文献   

The influence of road transport on the activities of glutathione reductase, glutathione peroxidase, and glutathione-S-transferase in equine erythrocytes     

Niedźwiedź A  Nicpoń J  Zawadzki M  Służewska-Niedźwiedź M  Januszewska L 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2012,41(1):123-126

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Yellow melon yield in response to mineral or organic fertilization     

Jaciane Rosa Maria de Souza  Adriana Guirado Artur  José Israel Pinheiro 《Journal of plant nutrition》2018,41(9):1197-1204

Although current recommendations in Brazil suggest the application of mineral and organic fertilizers, there is little information regarding the interaction between them in melon plants. This study aimed to evaluate the effects of mineral and organic fertilization in the plant development, nutritional status, and fruit yield of yellow melon. The following fertilizations were evaluated: mineral fertilization; bovine manure; bovine manure associated with mineral fertilization; poultry litter; and poultry litter associated with mineral fertilization. Bovine manure and poultry litter were applied at rates equivalent to 15 m³ ha^?1 and 5 m³ ha^?1, respectively. Phosphorus and potassium were applied based on the results of the soil analysis and nitrogen based on the expected yield of 20 to 30 t ha^?1 of fruits. Phosphate and organic fertilizers were applied at the time of preparation of the beds, while nitrogen and potassium were supplied daily through irrigation water. Goldex F1 melon hybrid seedlings were used in the experiment. Plants were collected to evaluate the dry matter production at beginning of flowering, beginning of fruiting, fruit growth, and fruit harvest. Leaves were collected at flowering stage to evaluate the nutritional status of plants. Harvest was made when the fruits reached the intense yellow coloration. Organic fertilizers alone and combined to mineral fertilizer did not affect the dry matter yield of leaves, stems, fruits and shoot, the concentrations of macro and micronutrients in melon leaves, the nitrogen accumulation in the plant, and the yield of the yellow melon. The application of bovine manure associated to mineral fertilization showed the lowest values of nitrogen recovery applied. Bovine manure application resulted in increase of nitrogen organic forms in soil.  相似文献