Colonization and growth promotion characteristics of Enterobacter sp. and Herbaspirillum sp. on Brassica oleracea   总被引：1，自引：0，他引：1  

Muhammad ZAKRIA  Asuka OHSAKO  Yuichi SAEKI  Akihiro YAMAMOTO  Shoichiro AKAO 《Soil Science and Plant Nutrition》2008,54(4):507-516

We investigated the effects of inoculating a dicot plant, Brassica oleracea , with nitrogen-fixing endophytic bacteria isolated from monocots. The bacteria used were Enterobacter sp. strain 35 isolated from sugarcane and Herbaspirillum sp. strain B501 isolated from wild rice. Under glasshouse conditions, B. oleracea inoculated with strain 35 had a significantly greater fresh weight than uninoculated plants, and the fresh weight of plants inoculated with strain B501 tended to be higher than that of uninoculated plants. We conducted a laboratory-scale experiment to confirm the above results and to investigate the colonization and nitrogen-fixing abilities of these bacteria. Plants inoculated with Enterobacter sp. strain 35-1, a green fluorescent protein (GFP)-labeled strain derived from strain 35, had larger bacterial populations and greater acetylene reduction activity than those inoculated with Herbaspirillum sp. strain B501 gfp 1. Strain 35-1 colonized the intercellular spaces and the junctions of the lateral roots with the parent root. The results indicated that isolates from monocots can successfully colonize B. oleracea (a dicot) and promote plant growth.  相似文献   

Transpiration and canopy conductance at two slope positions in a Japanese cedar forest watershed   总被引：1，自引：0，他引：1  

Tomo&#x;omi Kumagai  Makiko Tateishi  Takanori Shimizu  Kyoichi Otsuki 《Agricultural and Forest Meteorology》2008,148(10):1444-1455

Plant–soil system patterns and processes along a slope are among the greatest causes of uncertainty in estimating watershed-scale transpiration (E). Tree-to-tree and radial variations in xylem sap flux density (F_d), in addition to tree biometrics, were measured over a 2-year period (2005–2006) in two slope stand positions. The areas of interest consisted of an upper slope plot (UP) and a lower slope plot (LP) in a Japanese cedar (Cryptomeria japonica D. Don) forest watershed and the environmental controls of stand E for each plot were compared. Canopy stand E (E_C) and canopy stomatal conductance (G_C) in the UP were less than those in the LP during the growing season, while those in the UP were greater than those in the LP over winter. In addition, mean stand F_d (J_S) in the UP was greater than that in the LP over winter, but J_S values were similar in the UP and LP except in the winter, which allows us to extrapolate watershed-scale E based on J_S estimated from F_d measurements of a partial stand in the watershed. However, this relationship contains a bias and differed between 2005 and 2006. Although there were significant differences in soil moisture conditions between the UP and LP in both years, a systematic relationship between the similarity in J_S and soil moisture conditions was not found. The bias was due to a tendency for J_S in the LP to be greater than that in the UP in 2006. This tendency was amplified because J_S in the LP was greater than that in the UP around an atmospheric humidity deficit (D) of 1–1.5 kPa and frequencies of this D range were higher in 2006 than in 2005. The greater J_S in the LP at D  1–1.5 kPa could be explained by the difference in the response of G_C to D between the UP and LP. Our results suggest this to be the cause of the similarity in J_S values for the UP and LP and for the occasional abortion of its similarity. However, even when the bias or the occasional deviation is disregarded, the error in estimating stand E from a partial stand is so small that it is comparable to an F_d measurement error. For example, the error when using only the LP was 6.6% for stand E.  相似文献   

The multiplication of transmissible gastroenteritis viruses in several cell lines originated from porcine kidney and effects of trypsin on the growth of the viruses   总被引：1，自引：0，他引：1  

E Honda  H Takahashi  K Okazaki  T Minetoma  T Kumagai 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1990,52(2):217-224

Plaque formation, replication and related cytopathic function of 9 strains of transmissible gastroenteritis (TGE) virus were examined in primary cells and cell lines such as CPK, IB-RS-2, ESK, and PK-15 originated from porcine kidney and the effects of trypsin on the replication of TGE virus were examined in CPK cells. All strains produced a cytopathic effect and grew well in CPK cells as well as in primary porcine kidney cells. The effect of trypsin on the plaque formation was different from strains. The number of plaques produced by strains TO-163, Ukiha and Niigata increased from 2.6 to 3.52 times when trypsin was present in the medium during incubation at 37 degrees C for 1 hr after adsorption of the virus at 4 degrees C for 2 hr. The plaque sizes of TO-163, h-5, Ukiha and Niigata became larger from 1.4 to 1.7 times, when trypsin was present in the agar MEM overlay.  相似文献   

Effect of an adenoviral vector that expresses the canine p53 gene on cell growth of canine osteosarcoma and mammary adenocarcinoma cell lines     

Yazawa M  Setoguchi A  Hong SH  Uyama R  Nakagawa T  Kanaya N  Nishimura R  Sasaki N  Masuda K  Ohno K  Tsujimoto H 《American journal of veterinary research》2003,64(7):880-888

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Effects of bovine lactoferrin by the intramammary infusion in cows with staphylococcal mastitis during the early non-lactating period     

Kai K  Komine Y  Komine K  Asai K  Kuroishi T  Kozutsumi T  Itagaki M  Ohta M  Kumagai K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(10):873-878

To evaluate the clinical effects of bovine lactoferrin on staphylococcal mastitis in Holstein cows during the early non-lactating period, 41 mammary quarters were selected randomly from 36 cows on 3 dairy farms. Twelve quarters were infused intramammarily with bovine lactoferrin. Twenty-nine quarters were infused with antibiotic as a control. In the bovine lactoferrin-infused group, 91.7% of mastitic quarters were cured at 7 days after calving, compared with 48.3% in the control group. Furthermore, the changes in mammary secretion induced by the infusion of bovine lactoferrin were investigated. Mean numbers of staphylococci in mammary gland secretions were significantly decreased in both 5 bovine lactoferrin-infused quarters and 5 antibiotic-infused control quarters (p<0.05). Unlike in the control quarters, the mean total cell concentration in the mammary gland secretions increased in bovine lactoferrin-infused quarters. Similar results were obtained in 6 healthy quarters which were infused with bovine lactoferrin. In these quarters, the cell population contained mainly phagocytes such as polymorphonuclear leukocytes and cells positive for CD11b which is known as a complement receptor. The mean concentration of C3 in mammary gland secretions was significantly increased in 5 mastitic quarters infused with bovine lactoferrin (p<0.05), but showed no significant change in 5 mastitic control quarters. These results suggested that bovine lactoferrin treatment for staphylococcal mastitis in the early non-lactating period might increase the rate of cure through the induction of innate immunity in the host.  相似文献   

Residues of aflatoxins in the liver,muscle and eggs of domestic fowls     

Bintvihok A  Thiengnin S  Doi K  Kumagai S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(11):1037-1039

The residue of aflatoxins in the liver, muscle and eggs of laying ducks, hens and quails and in broiler chickens was examined by conducting 7-day feeding experiments with a diet containing 3 ppm Aflatoxin B1 (AFB1). Birds were sacrificed on the 8th or 11th day of AFB1 feeding. AFB1 and its metabolites in the tissues and eggs were determined by HPLC. The tissue levels of AFB1 and its metabolites were higher in quail than in the other birds. The levels of AFB1 and its metabolites, including acid-hydrolyzable metabolites, were more than 10-fold higher in the liver than in the muscle in all the species. The ratio of AFB1 in the feed to the residual level in the liver was 383 in quail, but was > or = 5769 in the other birds. The corresponding ratios in the egg yolk and albumen of AFB1 were 4615 and 3846, respectively, in chicken hens, and these values were higher than those in the other birds.  相似文献   

Effects of feeding polyphenol‐rich winery wastes on digestibility,nitrogen utilization,ruminal fermentation,antioxidant status and oxidative stress in wethers          下载免费PDF全文

Kyohei Ishida  Yosuke Kishi  Kazato Oishi  Hiroyuki Hirooka  Hajime Kumagai 《Animal Science Journal》2015,86(3):260-269

Four wethers were used in a 4 × 4 Latin square design experiment to evaluate the availability of two types of winery wastes, winery sediment and grape pomace, as ruminant feeds possessing antioxidant activities. Each wether was assigned to one of the following four treatments: (i) 75 g/kg winery sediment (WS) on a dry matter (DM) basis; (ii) 166 g/kg DM winery grape pomace (WP); (iii) control diet (CD; 17 g/kg DM soybean meal);and (iv) only tall fescue hay (TFH; no additive). Winery sediment and grape pomace had high levels of polyphenols and of radical scavenging activities. Feeding with winery sediment and grape pomace did not negatively affect the intake, but it depressed crude protein (CP) digestibility compared with CD (P = 0.052 and P < 0.01 for WS and WP, respectively). Polyphenols in winery wastes decreased ruminal ammonia production (P = 0.089 and P < 0.05), likely due to their inhibitive effect on microbial activities in the rumen. The addition of winery sediment and grape pomace decreased urinary 8‐hydroxy‐2'‐deoxyguanosine (8‐OHdG; an index of oxidative damages) excretion per day (P < 0.05 and P = 0.059). The results indicated that winery sediment and grape pomace could alter nitrogen metabolism and/or act as new antioxidants for ruminants.  相似文献   

Measurement of intestinal mucosal permeability in dogs with lymphocytic-plasmacytic enteritis     

Kobayashi S  Ohno K  Uetsuka K  Nakashima K  Setoguchi A  Fujino Y  Tsujimoto H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(7):745-749

Lymphocytic-plasmacytic enteritis (LPE) is a type of canine inflammatory bowel disease (IBD). One of its most probable causes is a defect in the mucosal permeability barrier. In the present study, intestinal permeability in LPE dogs was examinated to evaluate its clinical value. Twenty-nine dogs with LPE diagnosed by clinical and histological examinations were included in this study. Intestinal permeability was evaluated by measuring the ratio of the concentrations of two sugars (lactulose (L) and rhamnose (R)) with different molecular weights in urine samples after oral administration of a solution containing them. Biopsy specimens of duodenum were evaluated according to histological criteria. The urinary L:R ratio in the 29 LPE dogs (1.68 +/- 1.17, mean +/- SD) was significantly higher than that in the 10 healthy control dogs (0.75 +/- 0.38, P<0.01). In the LPE dogs, a weak correlation was observed between the histopathological grading score of the duodenum and the urinary L:R ratio (r=0.408, P<0.05). The urinary L:R ratio in the 20 dogs showing hypoalbuminemia (< 2.5 g/dl) was significantly higher than that in the 9 dogs with normal serum albumin levels > 2.5 g/dl (P<0.01). In conclusion, permeability of the intestinal mucosa as determined by the urinary L:R ratio could be a useful laboratory parameter for evaluating intestinal damage in LPE dogs.  相似文献   

An epidemic of swine influenza in Japan   总被引：1，自引：0，他引：1  

T Sugimura  T Ogawa  Y Tanaka  K Nishimori  T Kumagai  N Komatsu 《Research in veterinary science》1981,31(3):345-349

In a serological survey of pigs in Japan haemagglutination inhibition (HI) antibody to swine influenza virus (Hsw1N1) was first detected in 721 of 3313 (21.1 per cent) sera in 1977 and 1578 of 4946 (32.1 per cent) in 1978. The virus was prevalent in all districts of Japan but to a different extent. In contrast to the high incidence of antibody, there were few cases of infection with clinical signs and only 13 outbreaks of swine influenza were recognised in Japan from 1978 to 1979. Thirty-seven influenza viruses were isolated from nasal swabs of diseased pigs in 13 outbreaks. The subtype of most isolates was Hsw1N1 but two strains were Hsw1N2.  相似文献   

Cutaneous rhabdoid tumor in a cat     

Izawa T  Yamate J  Takeda S  Kumagai D  Kuwamura M 《Veterinary pathology》2008,45(6):897-900

Rhabdoid tumor is a highly aggressive neoplasm of unknown cellular origin in humans, usually occurring in the kidney and central nervous system of infants or children. In older patients, it occurs rarely in other organs, including the skin and soft tissues. A subcutaneous mass in a 13-year-old male mixed-breed cat was composed of nests or sheets of round to polygonal cells with glassy eosinophilic cytoplasmic inclusions. Immunohistochemically, many neoplastic cells expressed vimentin, localized to the cytoplasmic inclusions, whereas the cytoplasm of some neoplastic cells was diffusely positive for neuron-specific enolase, neurofilament, or S-100 protein. By electron microscopy, the cytoplasmic inclusions were found to be composed of aggregates of intermediate filaments. These findings are quite similar to the histologic, immunohistochemical, and ultrastructural features of human rhabdoid tumors and the few rhabdoid tumors reported in animals.  相似文献