Experimental Sarcocystis levinei infection in buffalo calves     

A K Jain  S L Gupta  R P Singh  S K Mahajan 《Veterinary parasitology》1986,21(1):51-53

Six buffalo calves were orally inoculated with 3 graded doses of sporocysts of Sarcocystis levinei (0.5, 1.0 and 2.0 million sporocysts; 2 calves for each dose) while two more calves were kept as uninoculated controls. One calf from each group was killed at 30 days post infection (DPI) and the other at 80 DPI. Inoculated calves showed a dose dependent response. The calves inoculated with 0.5 and 1.0 million sporocysts did not manifest any clinical signs of disease up to 80 DPI. One of the two calves inoculated with 2.0 million sporocysts showed clinical signs of weakness, emaciation and anaemia during the 5th week post infection. The other calf remained healthy until it was killed at 30 DPI. Pale liver tissue, gelatinization of fat and haemorrhages in the heart were observed in one calf inoculated with 2.0 million sporocysts; only microscopic lesions were seen in other calves. Schizonts and merozoites were not observed in any calf. Mature sarcocysts were observed in cardiac and skeletal muscle of calves killed at 80 DPI whereas no sarcocysts were seen in calves killed at 30 DPI.  相似文献   

Screening of water-efficient rice genotypes for dry direct seeding in South Asia     

Gulshan Mahajan  Kuldeep Singh  Naveen Singh  Rupinder Kaur  Bhagirath S Chauhan 《Archives of Agronomy and Soil Science》2018,64(1):103-115

Rice genotypes having high crop water productivity in dry direct-seeded rice (DDSR) need to be assessed while promoting labor- and water-saving technology. The objective of this study was to evaluate the ability of several selection indices to identify genotypes with high crop water productivity in DDSR. A set of 16 genotypes [13 recombinant inbred lines (RILs) and three genotypes as check for control] were evaluated under DDSR for two consecutive years (2013 and 2014) using two irrigation regimes [-10 kPa (non-stress) and -20 kPa (stress)]. Grain yield varied from 5.3 to 8.4 t ha^-1 and 5.9 to 8.7 t ha^-1 for irrigation regimes of -20 and -10 kPa, respectively. Yield reductions caused by water stress ranged from 2.9 to 25.1%; being lowest in RIL1649 and highest in hybrid SVH-127. The genotypes with high stress-tolerance index (STI), mean productivity (MP), geometric mean productivity (GMP) and harmonic mean productivity (HAR) were identified as the most productive genotypes for high crop water productivity in DDSR. The results implied that selection based on stress tolerance indices likes STI, MP, GMP and HAR was useful in identifying genotypes with high crop water productivity under DDSR and RIL 1649 and SVH-127 were identified as superior genotypes in this regard.  相似文献   

Construction of a Salmonella Gallinarum ghost as a novel inactivated vaccine candidate and its protective efficacy against fowl typhoid in chickens     

Chaudhari AA  Jawale CV  Kim SW  Lee JH 《Veterinary research》2012,43(1):44

In order to develop a novel, safe and immunogenic fowl typhoid (FT) vaccine candidate, a Salmonella Gallinarum ghost with controlled expression of the bacteriophage PhiX174 lysis gene E was constructed using pMMP99 plasmid in this study. The formation of the Salmonella Gallinarum ghost with tunnel formation and loss of cytoplasmic contents was observed by scanning electron microscopy and transmission electron microscopy. No viable cells were detectable 24 h after the induction of gene E expression by an increase in temperature from 37 °C to 42 °C. The safety and protective efficacy of the Salmonella Gallinarum ghost vaccine was tested in chickens that were divided into four groups: group A (non-immunized control), group B (orally immunized), group C (subcutaneously immunized) and group D (intramuscularly immunized). The birds were immunized at day 7 of age. None of the immunized animals showed any adverse reactions such as abnormal behavior, mortality, or signs of FT such as anorexia, depression, or diarrhea. These birds were subsequently challenged with a virulent Salmonella Gallinarum strain at 3 weeks post-immunization (wpi). Significant protection against the virulent challenge was observed in all immunized groups based on mortality and post-mortem lesions compared to the non-immunized control group. In addition, immunization with the Salmonella Gallinarum ghosts induced significantly high systemic IgG response in all immunized groups. Among the groups, orally-vaccinated group B showed significantly higher levels of secreted IgA. A potent antigen-specific lymphocyte activation response along with significantly increased percentages of CD4⁺ and CD8⁺ T lymphocytes found in all immunized groups clearly indicate the induction of cellular immune responses. Overall, these findings suggest that the newly constructed Salmonella Gallinarum ghost appears to be a safe, highly immunogenic, and efficient non-living bacterial vaccine candidate that protects against FT.  相似文献   

Toll pathway-dependent blockade of CD4+CD25+ T cell-mediated suppression by dendritic cells     

Pasare C  Medzhitov R 《Science (New York, N.Y.)》2003,299(5609):1033-1036

Toll-like receptors (TLRs) control activation of adaptive immune responses by antigen-presenting cells (APCs). However, initiation of adaptive immune responses is also controlled by regulatory T cells (TR cells), which act to prevent activation of autoreactive T cells. Here we describe a second mechanism of immune induction by TLRs, which is independent of effects on costimulation. Microbial induction of the Toll pathway blocked the suppressive effect of CD4+CD25+ TR cells, allowing activation of pathogen-specific adaptive immune responses. This block of suppressor activity was dependent in part on interleukin-6, which was induced by TLRs upon recognition of microbial products.  相似文献   

An allele-specific PCR assay for the rapid and serotype-specific detection of Salmonella pullorum   总被引：2，自引：0，他引：2  

Desai AR  Shah DH  Shringi S  Lee MJ  Li YH  Cho MR  Park JH  Eo SK  Lee JH  Chae JS 《Avian diseases》2005,49(4):558-561

Salmonella serovar Pullorum is a causative agent of pullorum disease (PD) in poultry and is responsible for severe economic losses to the poultry industry in many parts of the world. A definitive detection of Pullorum requires culture followed by serotyping and biochemical identification, a process that is tedious and takes several weeks to accomplish. We have developed a rapid allele-specific polymerase chain reaction (PCR) method based on the nucleotide polymorphism in rfbS gene sequence for the serotype-specific detection of Pullorum and its differentiation from the closely related Gallinarum. The specificity of this PCR assay was tested using DNA samples from Pullorum (n = 13), Salmonella serotypes other than Pullorum (n = 19), and closely related non-Salmonella organisms (n = 5). The PCR assay was highly serotype-specific as the PCR amplicon of 147 base pairs was observed only in the case of Pullorum, while all the other DNA samples tested PCR negative. A definitive identification of Pullorum cultures was possible in less than 3 hr. As little as 100 pg of SP DNA was detected. This allele-specific PCR method is highly specific as well as sensitive and may be an effective molecular tool in the rapid and serotype-specific detection of Pullorum and differentiation from other Salmonella species.  相似文献   

Isolation of Chlamydia from Pneumonic Lungs of Buffaloes,Cattle and Sheep     

P. N. Dhingra  L. P. Agarwal  V. M. Mahajan  S. C. Adalakha 《Zoonoses and public health》1980,27(8):680-682

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Parent—Offspring correlations and heritability of some characters in okra     

Yash Paul Mahajan  B.R. Sharma 《Scientia Horticulturae》1979,10(2):135-139

Four testers, 16 lines and 64 F₁'s were used in a line × tester analysis of okra (Abelmoschus esculentus L.). Parent—offspring correlations were high for yield per plant, number of ridges per fruit and fruit diameter with all the 4 testers. The heritability estimates were high for number of fruits, fruit length and fruit diameter. Yield had a positive and significant association with plant height, number of fruits per plant and fruit length, both in parents and hybrids. It was, therefore, suggested that number of fruits per plant and fruit length and diameter be given more consideration in selecting superior types in okra.  相似文献   

Medicine. The ultimate model organism     

Butte AJ 《Science (New York, N.Y.)》2008,320(5874):325-327

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Monitoring nitrogen,phosphorus and sulphur in hybrid rice (<Emphasis Type="Italic">Oryza sativa</Emphasis> L.) using hyperspectral remote sensing     

G. R. Mahajan  R. N. Pandey  R. N. Sahoo  V. K. Gupta  S. C. Datta  Dinesh Kumar 《Precision Agriculture》2017,18(5):736-761

Till date, the remote sensing research on crop nutrient monitoring has focused mainly on biomass and nitrogen (N) estimation and only a few attempts had been made to characterize and monitor macronutrients other than N. Field experiments were undertaken to study the remote detection of macronutrient status of rice using hyperspectral remote sensing. The variability in soil available N, phosphorus (P) and sulphur (S) and their content in plants were created using artificial fertility gradient design. The leaf and canopy hyperspectral reflectance was captured from variable macronutrient status vegetation. Linear correlation analysis between the spectral reflectance and plant nutrient status revealed significantly (p < 0.05) higher correlation coefficient at 670, 700, 730, 1090, 1260, 1460 nm for the nutrient under study. Published and proposed vegetation indices (VIs) were tested for canopy N, P and S prediction. The results of the investigation revealed that, published VIs (NDVI hyper and NDVI broadbands) could retrieve canopy N with higher accuracy, but not P and S. The predictability of the visible and short wave infrared based VI NRI₁₅₁₀ ((R₁₅₁₀ ? R₆₆₀)/(R₁₅₁₀ + R₆₆₀)) was the highest (r = 0.81, p < 0.01) for predicting N. Based on the outcomes of linear correlation analysis new VIs were proposed for remote detection of P and S. Proposed VI P_670_1260 ((R₁₂₆₀ ? R₆₇₀)/(R₁₂₆₀ + R₆₇₀)) retrieved canopy P status with higher prediction accuracy (r = 0.67, p < 0.01), whereas significantly higher canopy S prediction (r = 0.58, p < 0.01) was obtained using VI S_670_1090 ((R₁₀₉₀ ? R₆₇₀)/(R₁₀₉₀ + R₆₇₀)). The proposed spectral algorithms could be used for real time and site-specific N, P and S management in rice. Nutrient specific wavelengths, identified in the present investigation, could be used for developing relatively low-cost sensors of hand-held instruments to monitor N, P and S status of rice plant.  相似文献   

Surgical Management of Enterocutaneous Fistula in a Mare     

Vandana Sangwan  Jitender Mohindroo  Kiranjeet Singh  Mulinti Raghunath  Shashi Kant Mahajan 《Journal of Equine Veterinary Science》2010

Enterocutaneous fistulas are reported to be sequelae of congenital umbilical hernia or penetrating trauma to the equine patient's abdomen. An enterocutaneous fistula is considered to be the least common clinical presentation in a horse with incarcinated umbilical hernia, and may be life-threatening if not managed timely. This case report describes an enterocutaneous fistula in a mare as a sequela to congenital umblical hernia. The mare had an uneventful recovery after surgical repair and delivered a healthy foal on subsequent follow-up. The report highlights the need to repair congenital umbilical hernias (regardless of the size), if they do not resolve by the age of 6 to 12 months.  相似文献