Myoclonus and hypercalcemia in a dog with poorly differentiated lymphoproliferative neoplasia     

Cassia H. Z. Hare  Joy Archer  Emilie Cloup  Marie‐Aude Genain  Katherine Hughes  Katie E. McCallum  Lisa Alves 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2019,33(2):856-861

A 1‐year, 8‐month‐old Rhodesian Ridgeback was presented with obtundation, ambulatory tetraparesis, and myoclonus. Initial clinical findings included ionized hypercalcemia with an apparent marked increase in parathyroid hormone, thrombocytopenia, and nonregenerative anemia. Low numbers of circulating atypical cells were noted on blood film evaluation. Brain magnetic resonance imaging identified an extra‐axial contrast enhancing subtentorial lesion, and cerebrospinal fluid (CSF) analysis documented a marked atypical lymphocytic pleocytosis. Flow cytometry performed on the CSF demonstrated expression of only CD45, CD90, and MHC class II, with Pax5 positivity on subsequent immunohistochemistry. The final diagnosis was of B‐cell lymphoblastic lymphoma or acute leukemia, given the distribution of disease and the presence of significant bone marrow infiltration alongside an aggressive clinical course. The unusual immunophenotype of the neoplastic cells and hypercalcemia presented antemortem diagnostic challenges, highlighting the need for a multidisciplinary approach and caution in the interpretation of clinical abnormalities in cases with multiple comorbidities.  相似文献   

Endotracheal tube placement during computed tomography of brachycephalic dogs alters upper airway dimensional measurements          下载免费PDF全文

Nai‐Chieh Liu  Eileen L. Troconis  Matthew McMillan  Marie‐Aude Genain  Lajos Kalmar  David J. Price  David R. Sargan  Jane F. Ladlow 《Veterinary radiology & ultrasound》2018,59(3):289-304

Computed tomography (CT) is used to document upper airway lesions in dogs with brachycephalic obstructive airway syndrome. The presence of an endotracheal tube during CT scanning is often required for general anesthesia. We hypothesized that the endotracheal tube placement would change the soft tissue dimensions of the upper airway. The aims of this prospective, method comparison study were to evaluate the reliability of the previously reported upper airway CT measurements with endotracheal tube placement, and to propose measurements that are minimally affected by the endotracheal tube. Twenty brachycephalic dogs were included in this study. Each dog underwent head/neck CT with an endotracheal tube, followed by a second scan without the endotracheal tube. Ten measurements of the soft palate, nasopharynx, and trachea were performed. Tracheal dimension was significantly larger with the endotracheal tube compared to without, whereas the soft palate cross‐sectional area was significantly smaller with the endotracheal tube than without the endotracheal tube. The influence of the endotracheal tube on the caudal nasopharynx cross‐sectional (transverse‐sectional) area varied with a mean proportional absolute difference of 35%. Rostral soft palate thickness, tracheal perimeter, and cross‐sectional area of the rostral nasopharynx were the measurements least affected by the endotracheal tube (intraclass correlation coefficient = 0.964, 0.967, and 0.951, respectively). Therefore, we proposed that these three measurements may be most useful for future brachycephalic obstructive airway syndrome studies that require CT scanning of intubated animals. However, with endotracheal tube placement, measurements of soft palate length, caudal nasopharyngeal cross‐sectional area, and trachea height and width may not be reliable.  相似文献   

Four odontocete species change hearing levels when warned of impending loud sound          下载免费PDF全文

Paul E. NACHTIGALL  Alexander Ya. SUPIN  Aude F. PACINI  Ronald A. KASTELEIN 《Integrative zoology》2018,13(2):160-165

Hearing sensitivity change was investigated when a warning sound preceded a loud sound in the false killer whale (Pseudorca crassidens), the bottlenose dolphin (Tursiops truncatus), the beluga whale (Delphinaperus leucas) and the harbor porpoise (Phocoena phocoena). Hearing sensitivity was measured using pip‐train test stimuli and auditory evoked potential recording. When the test/warning stimuli preceded a loud sound, hearing thresholds before the loud sound increased relative to the baseline by 13 to 17 dB. Experiments with multiple frequencies of exposure and shift provided evidence of different amounts of hearing change depending on frequency, indicating that the hearing sensation level changes were not likely due to a simple stapedial reflex.  相似文献   

Diversity of dead wood inhabiting fungi and bryophytes in semi-natural beech forests in Europe     

P. Ódor  J. Heilmann-Clausen  E. Aude  A. Piltaver  M.T. Veerkamp  T. Standovár  J. Kosec  H. Kraigher 《Biological conservation》2006,131(1):58-71

Saproxylic organisms are among the most threatened species in Europe and constitute a major conservation problem because they depend on the most important forestry product - dead wood. Diversity of fungal and bryophyte communities occurring on dead beech trees was analyzed in five European countries (Slovenia, Hungary, The Netherlands, Belgium and Denmark) considering tree level species richness (TLSR), country level species richness (CLSR), frequency distributions of species, occurrence of threatened species and relations between TLSR and decay stage, tree size and countries. Altogether 1009 trees were inventoried in 19 beech dominated forest reserves.The number of fungi in the full dataset was approximately three times larger (456 versus 161 species) and the proportion of low frequent species was higher than among bryophytes. The species richness of bryophytes and fungi was significantly different among countries considering both TLSR and CLSR. In addition the diversity patterns deviated considerably between the two groups of organisms. Slovenian sites appeared to be biodiversity hotspots for bryophytes characterized by high TLSR and CLSR and a high fraction of threatened species. Hungarian sites had somewhat lower bryophyte diversity, while the Atlantic region had deteriorated assemblages. Fungal species richness was very high in Denmark, but the Hungarian and Slovenian sites were richer in threatened and low frequency species. Tree size was better able to explain variation in TLSR in both organism groups than decay stage. TLSR was found to vary significantly between countries but the difference was most considerable in the case of bryophytes.The diversity patterns of both organism groups along the investigated geographical gradient appear to be influenced by both climatic and management related factors (forest history, dead wood availability and continuity, habitat fragmentation). There is no doubt that an increase in the abundance of dead wood in European beech forests will benefit diversity of saproxylic fungi and bryophytes, especially if a continuous presence of large diameter logs are secured within individual stands.  相似文献   

Spatiotemporal dynamics of the agricultural landscape mosaic drives distribution and abundance of dominant carabid beetles     

Ronan Marrec  Gaël Caro  Paul Miguet  Isabelle Badenhausser  Manuel Plantegenest  Aude Vialatte  Vincent Bretagnolle  Bertrand Gauffre 《Landscape Ecology》2017,32(12):2383-2398

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Are similar landscapes the result of similar histories?     

Aude Ernoult  Sylviano Freiré-Diaz  Estelle Langlois  Didier Alard 《Landscape Ecology》2006,21(5):631-639

This landscape study was based on the sampling of 20 replicated landscape sites (1 km² each) that were located within the floodplain of the river Seine. For each site, 13 landscape variables were measured at three dates (1963–1985–2000). The aim of this study was to investigate the overall landscape variability through its different dimensions (space vs. time) and to assess the relative importance of each dimension. We used a new statistical method, i.e., partial triadic analysis (PTA), which allowed us to assess both (1) the spatial variability of the floodplain landscape and its dynamics in time and (2) the dynamic trajectories of the landscape variables for each site. The results showed, at the floodplain scale, the same landscape pattern has emerged since 1963, although a major trend was observed which consisted in a decrease in meadows resulting from an increase in arable crops. At the site scale, landscape sites, even if they were all influenced by this general trend during the 40-year period, showed contrasting trajectories. These results suggest that similar sites in 2000 do not necessarily share common histories and that contrasting sites in 2000 may have originated from similar patterns in 1963. The issue of biodiversity surrogates is then discussed, suggesting that new landscape metrics should be developed, emphasising spatial variability and (or) temporal dynamics.  相似文献   

Performance of diagnostic tests for the detection and identification of <Emphasis Type="Italic">Pseudomonas syringae</Emphasis> pv. <Emphasis Type="Italic">actinidiae</Emphasis> (Psa) from woody samples     

Stefania Loreti  Amandine Cunty  Nicoletta Pucci  Aude Chabirand  Emilio Stefani  Adela Abelleira  Giorgio M. Balestra  Deirdre A. Cornish  Francesca Gaffuri  Davide Giovanardi  Richard A. Gottsberger  Maria Holeva  Aynur Karahan  Charikleia D. Karafla  Angelo Mazzaglia  Robert Taylor  Leonor Cruz  Maria M. Lopez  Joel L. Vanneste  Françoise Poliakoff 《European journal of plant pathology / European Foundation for Plant Pathology》2018,152(3):657-676

The aim of this study was to characterise the performance of new molecular methods for the detection and identification of Pseudomonas syringae pv. actinidiae (Psa) and to provide validation data in comparison to the assays mentioned in official diagnostic protocols and being currently used. Eleven molecular tests for the Psa detection were compared in an inter-laboratory comparison where each laboratory had to analyse the same panel of samples consisting of thirteen Psa-spiked kiwifruit wood extracts. Laboratories had to perform also isolation from the wood extracts. Data from this interlaboratory test performance study (TPS) was statistically analysed to assess the performance of each method. In order to provide complete validation data, both for detection and identification, this TPS was supplemented by a further study of identification from pure culture of phylogenetically closely related Pseudomonas spp., Psa, and bacterial strains associated with kiwifruit. The results of both these studies showed that simplex-PCRs gave good results, whereas duplex-PCR and real-time PCR were the most reliable tools for detection and identification of Psa. Nested and multiplex-PCR gave false-positive results. The use of the most reliable detection test is suggested for routine analyses, but when Psa-free status needs to be accurately assessed, it is recommended that at least two detection tests are used. This work provides a wide comparison of the available diagnostic methods, giving new information for a possible revision of the official diagnostic protocols (e.g. European and Mediterranean Plant Protection Organization (EPPO) protocol PM7/120 for the detection of Psa).  相似文献   

Aggregation of a proline-rich protein induced by epigallocatechin gallate and condensed tannins: effect of protein glycosylation     

Pascal C  Poncet-Legrand C  Cabane B  Vernhet A 《Journal of agricultural and food chemistry》2008,56(15):6724-6732

Astringency is one of the most important organoleptic qualities of numerous beverages, including red wines. It is generally thought to originate from interactions between tannins and salivary proline-rich proteins (PRPs). In this work interactions between a glycosylated PRP, called II-1, and flavan-3-ols were studied in aqueous solutions and at a colloidal level, by dynamic light scattering (DLS) and small-angle X-ray scattering (SAXS). The flavan-3-ols were a monomer, epigallocatechin gallate (EGCG), and polymerized flavan-3-ol fractions extracted from grape seeds. In aqueous solutions containing EGCG and protein II-1, protein aggregation took place when protein concentration and the EGCG/protein ratio exceeded a threshold. The aggregates had a small size, comparable with the dimensions of protein monomers, and formed stable dispersions (no phase separation). Most proteins remained free in solution. This behavior is in sharp contrast with the phase separation observed for nonglycoslated PRP in the same conditions. Moreover, this slight aggregation of II-I in the presence of EGCG was disrupted by the addition of 12% ethanol. Increasing the flavan-3-ol molecular weight strongly enhanced II-I/tannin aggregation: the threshold was at a lower protein concentration (0.2 mg/mL) and a lower tannin/protein ratio. Still, in most cases, and in contrast with that observed with a nonglycosylated PRP, the aggregates remained of discrete size and stable. Only at low ethanol content (2%) did the addition of tannin polymers finally lead to phase separation, which occurred when the molar ratio of tannins to proteins exceeded 12. This systematic effect of ethanol confirmed the strong effect of cosolvents on protein/tannin interactions.  相似文献   

Phase II Study of Biweekly Plitidepsin as Second-Line Therapy for Advanced or Metastatic Transitional Cell Carcinoma of the Urothelium     

Herlinde Dumez  Enrique Gallardo  Stephane Culine  Joan Carles Galceran  Patrick Sch?ffski  Jean P. Droz  Sonia Extremera  Sergio Szyldergemajn  Aude Fléchon 《Marine drugs》2009,7(3):451-463

The objective of this exploratory, open-label, single-arm, phase II clinical trial was to evaluate plitidepsin (5 mg/m²) administered as a 3-hour continuous intravenous infusion every two weeks to patients with locally advanced/metastatic transitional cell carcinoma of the urothelium who relapsed/progressed after first-line chemotherapy. Treatment cycles were repeated for up to 12 cycles or until disease progression, unacceptable toxicity, patient refusal or treatment delay for >2 weeks. The primary efficacy endpoint was objective response rate according to RECIST. Secondary endpoints were the rate of SD lasting ≥ 6 months and time-to-event variables. Toxicity was assessed using NCI-CTC v. 3.0. Twenty-one patients received 57 treatment cycles. No objective tumor responses occurred. SD lasting <6 months was observed in two of 18 evaluable patients. With a median follow-up of 4.6 months, the median PFR and the median OS were 1.4 months and 2.3 months, respectively. The most common AEs were mild to moderate nausea, fatigue, myalgia and anorexia. Anemia, lymphopenia, and increases in transaminases, alkaline phosphatase and creatinine were the most frequent laboratory abnormalities. No severe neutropenia occurred. Treatment was feasible and generally well tolerated in this patient population; however the lack of antitumor activity precludes further studies of plitidepsin in this setting.  相似文献   

Design of intercrop management plans to fulfil production and environmental objectives in vineyards     

Aude Ripoche  Florian Celette  Jean-Pierre Cinna  Christian Gary 《European Journal of Agronomy》2010,32(1):30-39

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