Promotive effects of a 5-aminolevulinic acid-based fertilizer on growth of tissue culture-derived date palm plants (Phoenix dactylifera L.) during acclimatization     

Mohamed A. Awad 《Scientia Horticulturae》2008

The low survival rate together with the slow growth rate of the survived tissue culture-derived date palm plants during acclimatization are critical problems facing the tissue culture as a commercial technique of date palm propagation. Growth parameters, pigment contents and nutrient uptake were examined in tissue culture-derived date palm plants (Phoenix dactylifera L.) c.v. ‘Khalas’ subject to different concentration of a 5-aminolevulinic acid-based fertilizer, commercially known as Pentakeep-v, during acclimatization. Pentakeep application at 0.02, 0.04 or 0.08% at 10 days interval significantly enhanced growth at both VP2- and VP3-stage of acclimatization. However, such effects were not detectable at the VP1-stage. At the end of this stage, the successful plants ranged from 66.6 to 78.3% with no significant differences between the Pentakeep treatments and the control. All successful plants at this stage survived both the VP2 and the VP3 stages of acclimatization. Plants treated with Pentakeep, especially at 0.04 and 0.08%, developed faster and were ready for transplanting to the nursery or to the open field conditions about 4–5 months earlier than the control or those treated with 0.02% Pentakeep. Pentakeep significantly increased chlorophyll a contents in all treatments which was subsequently reflected on total chlorophyll and chlorophyll a/chlorophyll b ratio. Pentakeep application, especially at the 0.08%, significantly increased the concentration of N, Cu and Zn in the leaves of treated plants. The concentration of available P and Zn in the soil was significantly higher in the Pentakeep treatments than the control.  相似文献   

Proteolytic activities of suparen and rennilase on buffalo, cow, and goat whole casein and beta-casein.     

S Awad  Q Q Lüthi-Peng  Z Puhan 《Journal of agricultural and food chemistry》1999,47(9):3632-3639

The proteolytic specificity and activity of Mucor miehei protease (Rennilase) and Endothia parasitica protease (Suparen) on buffalo, cow, and goat whole casein and beta-casein (CN) were studied by analyzing the degradation products. The results suggest that Rennilase hydrolyzes casein of the three species in a manner similar to that of chymosin, resulting in the formation of alpha(s1)-I and beta-I, -II, -III as initial degradation fragments of alpha(s1)- and beta-CN. alpha(s1)-I was also the initial breakdown product of alpha(s1)-CN by Suparen. Contrary to Rennilase, Suparen showed a higher affinity toward beta-CN and hydrolyzes beta-CN, giving rise to degradation products characterized by mobility lower than that of beta-CN. Increasing NaCl concentration (>3%) reduced the proteolysis of beta-CN of the three species by Rennilase but not by Suparen. The hydrolysis of alpha(s1)-CN and alpha(s1)-I by the two enzymes was enhanced in the presence of NaCl.  相似文献   

Life history of Proprioseiopsis badri feeding on four eriophyid mite species (Acari: Phytoseiidae and Eriophyidae)     

Faten M. Momen  Abd-Elsatar M. Metwally  Abd Elrady K. Nasr  Awad A. Abdallah  Khaled M. Saleh 《Phytoparasitica》2014,42(1):23-30

The influence of four eriophyid mite species as prey, Aceria dioscoridis, Aceria olivi, Aculops lycopersici and Cisaberoptus kenyae, on the biology and demographic parameters of the predatory mite Proprioseiopsis badri, was studied under laboratory conditions. Feeding on A. dioscoridis and A. olivi enhanced the development of P. badri, resulted in the longest mean generation time and were the most suitable foods for the oviposition of the predator, as exhibited by the highest fecundity, net reproductive rate and gross reproductive rate. Preying on A. lycopersici gave the lowest fecundity, net and gross reproductive rates and finite rate of increase; therefore, this prey was the least suitable for the oviposition of P. badri. Preying on A. dioscoridis and A. olivi produced higher intrinsic rates of increase, reproduction rates and finite rates of increase for the predator in comparison with A. lycopersici and C. kenyae, which had the lowest value. These differences in response to various eriophyid pests should be considered for the production of healthy cultures of P. badri.  相似文献   

Possible effect of lesser galangal (Alpinia officinarum) extracts encapsulated into mesoporous silica nanoparticles on the immune status of rainbow trout (Oncorhynchus n)     

Elham Awad  Ahmed El‐Fiqi  Dawn Austin  Alastair Lyndon 《Aquaculture Research》2020,51(9):3674-3684

Using plant extracts as immunostimulants in aquaculture has prove effectiv in resisting infectious disease, in addition to being safe and inexpensive. The present study is concerned about the prospective mode of action of using lesser galangal extract (Alpinia officinarum) encapsulated into amine surface functionalized mesoporous silica nanoparticles (MSN) in order to elevate the immune status of rainbow trout (Oncorhynchus mykiss). Fish were randomly divided into 6 groups and fed for 4 weeks with commercial diets supplemented with 0% (control), 0.5% and 1.5% of lesser galangal extract, and with previous concentrations encapsulated with MSN (0.5% + MSN, 1.5% + MSN) and MSN (without extract). The effects of the feeding trial on the innate humoural immune parameters (total protein, myeloperoxidase content, lysozyme antiproteases and bactericidal activities) in rainbow trout were examined. Also, the influence of dietary supplement on some immune‐related genes in rainbow trout head kidney (TNF, IL‐8, IL‐1b, LYZ2 and CD4) after challenge with Yersinia ruckeri was examined. The results revealed enhancement in immune parameters in all treatment groups compared to the control, especially in the fish group fed with 1.5% + MSN which showed the highest significant difference (p < .05) in total protein, lysozyme and antiproteases activities. Also, feeding lesser galangal extract encapsulated into MSN led to an increase in myeloperoxidase content and bactericidal activity. An improvement in the expression of immune‐related genes has been recorded in fish groups fed doses of lesser galangal extract and lesser galangal extract encapsulated into MSN compared to the control or to the group fed with MSN only. Particularly, the group fed with 0.5% + MSN showed a significant up‐regulation in most of the immune‐related genes. The current investigation supports using lesser galangal extract encapsulated into MSN in fish diets as a supplement to enhance the immune response of rainbow trout and elevate its resistance to infectious diseases.  相似文献   

Differential replication properties among H9N2 avian influenza viruses of Eurasian origin     

Rokshana Parvin  Awad A. Shehata  Kristin Heenemann  Malgorzata Gac  Antje Rueckner  Mohammad Y. Halami  Thomas W. Vahlenkamp 《Veterinary research》2015,46(1)

Avian influenza H9N2 viruses have become panzootic in Eurasia causing respiratory manifestations, great economic losses and occasionally being transmitted to humans. To evaluate the replication properties and compare the different virus quantification methods, four Eurasian H9N2 viruses from different geographical origins were propagated in embryonated chicken egg (ECE) and Madin-Darby canine kidney epithelial cell systems. The ECE-grown and cell culture-grown viruses were monitored for replication kinetics based on tissue culture infectious dose (TCID₅₀), Hemagglutination (HA) test and quantitative real time RT-PCR (qRT-PCR). The cellular morphology was analyzed using immunofluorescence (IF) and cellular ELISA was used to screen the sensitivity of the viruses to amantadine. The Eurasian wild type-H9N2 virus produced lower titers compared to the three G1-H9N2 viruses at respective time points. Detectable titers were observed earliest at 16 h post inoculation (hpi), significant morphological changes on cells were first observed at 32 hpi. Few nucleotide and amino acid substitutions were noticed in the HA, NA and NS gene sequences but none of them are related to the known conserved region that can alter pathogenesis or virulence following a single passage in cell culture. All studied H9N2 viruses were sensitive to amantadine. The G1-H9N2 viruses have higher replication capabilities compared to the European wild bird-H9N2 probably due to their specific genetic constitutions which is prerequisite for a successful vaccine candidate. Both the ECE and MDCK cell system allowed efficient replication but the ECE system is considered as the better cultivation system for H9N2 viruses in order to get maximum amounts of virus within a short time period.  相似文献   

抗逆高羊茅新品种黔草1号的特征特性   总被引：1，自引：2，他引：1  

吴佳海  牟琼  罗天琼  唐成斌  尚以顺  莫本田  瓦庆荣 《种子》2007,26(12):101-104

介绍了抗逆高羊茅新品种黔草1号(Festuca arundinacea Schreb.cv.Qiancao No.1)的育种过程及其优良特性。在贵州省及四川、广西、新疆等地种植的种子平均产量比对照品种凌志和原始群体高出20%以上。返青期比引进品种凌志提前7天,枯黄期晚3天;是目前我国育成的自主知识产权优良抗逆草坪型新品种,是运动场草坪、环境绿化、生态治理和改良天然草场的理想草种之一。适宜我国长江中下游低山、丘陵等地区种植。  相似文献   

Prevalence and genetic diversity of Babesia and Anaplasma species in cattle in Sudan     

Awad H  Antunes S  Galindo RC  do Rosário VE  de la Fuente J  Domingos A  El Hussein AM 《Veterinary parasitology》2011,181(2-4):146-152

Disease prevalence studies are one of the most valuable tools to demonstrate the risk or impact of certain infections in local and global economies. The data obtained in these studies contribute to develop strategies for disease control. The present study aims to provide information about the prevalence of babesiosis and anaplasmosis in the northern regions of Sudan. Blood samples from four different states of Sudan were collected from apparently healthy cattle (n=692), DNA was extracted and the prevalence of Babesia and Anaplasma species was analyzed by PCR. The results confirmed the presence of Babesia bigemina, Babesia bovis and Anaplasma marginale in cattle in northern Sudan with overall prevalence rates of 4.0%, 1.9% and 6.1%, respectively. Statistical analysis revealed that the prevalence of B. bigemina, B. bovis and A. marginale varies significantly between Sudanese states as well as in different age groups, while gender seems not to have a significant effect on the prevalence of these pathogens among Sudanese cattle. The highest prevalence for B. bigemina was found in the Aljazirah State while the highest number of A. marginale positive samples was reported in River Nile.  相似文献   

5-Aminolevulinc acid increases tree yield and improves fruit quality of ‘Rabia’ and ‘Sukkariat-Yanbo’ date palm cultivars under hot arid climate     

Adel D. Al-Qurashi  Mohamed A. Awad 《Scientia Horticulturae》2011

Date palms growing in hot arid regions are generally facing several kinds of environmental stresses which limit tree growth and productivity and negatively affect fruit quality. During 2009 and 2010 seasons, the effects of 5-aminolevulininc acid (5-ALA) application at different rates on yield, fruit quality, pigment contents and nutrient uptake of ‘Rabia’ and ‘Sukkariat-Yanbo’ date palm cultivars were studied. In both cultivars, the application of 5-ALA significantly increased bunch weight and yield per tree compared to control. The highest bunch weight and yield per tree was achieved by 5-ALA at 200 ppm followed by 250 ppm applied on both fruit bunches and leaves. These were reflected on higher fruit and flesh weight and fruit length, at the bisir and rutab stages. However, the application of 5-ALA at 250 ppm only on fruit bunches without leaves significantly increased bunch weight and yield per tree compared to control only in ‘Sukkariat-Yanbo’ but not in ‘Rabia’ cultivar. There were significant interaction effects between treatments and seasons on bunch weight and yield in ‘Rabia’ but not in ‘Sukkariat-Yanbo’ cultivar. The rutab % was not significantly affected by any of the applied treatments and it was higher in 2009 than in 2010 season in ‘Rabia’, however, the opposite was true in ‘Sukkariat-Yanbo’ cultivar. Most of the physical parameters of fruit quality at both the bisir and rutab stages were significantly improved in both cultivars by 5-ALA application compared to control, in contrast to the biochemical parameters that were not affected. In both cultivars, chlorophyll a concentration significantly increased by 5-ALA application especially at 200 and 250 ppm on both leaves and fruits compared to control. While chlorophyll b concentration was not significantly affected by the 5-ALA application, total chlorophyll concentration was significantly higher at 250 ppm 5-ALA application than control in both cultivars. There were no significant differences among the different rates of 5-ALA application. Chlorophyll a/b ratio was not significantly affected by 5-ALA application compared to control. The concentrations of N, P and K in the leaves of both cultivars were not significantly affected by the 5-ALA application compared to control. It was concluded that 5-ALA application has promotive effects on yield, fruit quality and chlorophyll concentration of both ‘Rabia’ and ‘Sukkariat-Yanbo’ date palms under hot arid climate.  相似文献   

Metaphyseal osteomyelitis in an immature Abyssinian cat     

WA BRADLEY 《Australian veterinary journal》2003,81(10):608-608

Metaphyseal osteomyelitis is a rarely described condition in small animals, especially the cat. Infection, most commonly Staphylococcus sp, is considered to occur in the metaphyseal region of the immature animal due to vascular anomalies that predispose to the haematogenous seeding of bacteria in this area. There is also speculation that the characteristics of the bacteria that allow them to adhere to cartilage matrix, rather than vascular linings and erythrocytes, may provide an advantage for colonisation in the metaphysis, resulting in infection. This case describes the successful management of a case of distal radial metaphyseal osteomyelitis in an immature cat using surgical intervention and antibacterial therapy.  相似文献   

鸡新城疫病毒HN基因亚单位疫苗诱导免疫保护的实验研究   总被引：3，自引：0，他引：3  

丁壮  金宁一  王兴龙  米志强  夏志平  王承宇 《动物医学进展》2002,23(1):49-51

实验分别将应用Bac to Bac系统表达的新城疫病毒四平株和长春株血凝素-神经氨酸酶基因（HN基因）蛋白的重组杆状病毒感染Sf-9昆虫细胞后28℃培养72h后，洗涤、收集昆虫细胞，离心浓缩，-20℃冻融3次，用HA-HI实验和HN单抗中和试验测定表达产物的活性，将表达的重组蛋白作为亚单位疫苗免疫鸡，用间接ELISA和HI试验测定鸡体内抗体效价，免疫后第15d用国家标准强毒NDVF48E8攻毒，统计免疫保护率，结果表明，表达的NDVHN重组蛋白能够诱导鸡体产生抗NDV特异性IgG抗体和HI抗体，实验Ⅰ组（四平株）雏鸡保护率为65%，实验Ⅱ组（长春株）雏鸡保护率为100%。  相似文献