No effect of exogenous melatonin on development of cryopreserved metaphase II oocytes in mouse     

Wei Li  Keren Cheng  Yue Zhang  Qinggang Meng  Shi’en Zhu  Guangbin Zhou 《畜牧与生物技术杂志(英文版)》2015,6(1)

Background

This study was conducted to investigate effect of exogenous melatonin on the development of mouse mature oocytes after cryopreservation.

Results

First, mouse metaphase II (MII) oocytes were vitrified in the open-pulled straws (OPS). After warming, they were cultured for 1 h in M₂ medium containing melatonin at different concentrations (0, 10⁻⁹, 10⁻⁷, 10⁻⁵, 10⁻³ mol/L). Then the oocytes were used to detect reactive oxygen species (ROS) and glutathione (GSH) levels (fluorescence microscopy), and the developmental potential after parthenogenetic activation. The experimental results showed that the ROS level and cleavage rate in 10⁻³ mol/L melatonin group was significantly lower than that in melatonin-free group (control). The GSH levels and blastocyst rates in all melatonin-treated groups were similar to that in control. Based on the above results, we detected the expression of gene Hsp90aa1, Hsf1, Hspa1b, Nrf2 and Bcl-x1 with qRT-PCR in oocytes treated with 10⁻⁷, or 10⁻³ mol/L melatonin and untreated control. After warming and culture for 1 h, the oocytes showed higher Hsp90aa1 expression in 10⁻⁷ mol/L melatonin-treated group than in the control (P < 0.05); the Hsf1, Hsp90aa1 and Bcl-x1 expression were significantly decreased in 10⁻³ mol/L melatonin-treated group when compared to the control. Based on the above results and previous research, we detected the development of vitrified-warmed oocytes treated with either 10⁻⁷ or 0 mol/L melatonin by in vitro fertilization. No difference was observed between them.

Conclusions

Our results indicate that the supplementation of melatonin (10⁻⁹ to 10⁻³ mol/L) in culture medium and incubation for 1 h did not improve the subsequent developmental potential of vitrified-warmed mouse MII oocytes, even if there were alteration in gene expression.  相似文献   

Correlation between the serum and follicular fluid vascular endothelial growth factor A and nitric oxide levels and follicular vascularity in Arabian mares     

M.C. Gündüz  Ö. Turna  M. Uçmak  G. Kaşıkçı  S.Ö. Enginler  M. Erzengin  İ. Kurban  G. Evkuran  I. Akış  A. Ateş  A. Aktaş  İ. Sandal  Ö.B. Özdaş 《Journal of Equine Veterinary Science》2014

  相似文献   

Comparison of Cryoprotective Effects of Lycopene and Cysteamine in Different Cryoprotectants on Bull Semen and Fertility Results     

PB Tuncer  S Büyükleblebici  A Eken  U Taşdemir  E Durmaz  O Büyükleblebici  E Çoşkun 《Reproduction in domestic animals》2014,49(5):746-752

The objectives of this study were to compare glycerol and ethylene glycol at different concentrations as cryoprotectants and lycopene or cysteamine (with/without) as antioxidants in Tris extender for bull semen. Twenty‐four ejaculates were obtained from three bulls. Each ejaculate was split into four equal aliquots and diluted using both of the Tris extenders with glycerol (5% or 7%) or ethylene glycol (3% or 5%). After that, each extenders were split into three equal aliquots and added using both of the cysteamine 5 mm or lycopene 500 μg/ml, and control (without additives). The addition of 7% glycerol with cysteamine, 5% ethylene glycol with cysteamine and 3% ethylene glycol with cysteamine groups gave the lowest CASA motility than the other groups. However, 7% glycerol and 7% glycerol with lycopene resulted in a better rate of CASA progressive motility compared with that of other groups. Generally, all the lycopene groups signed better protective effects on acrosome and total morphology than the other groups. Glycerol 7% and 3% ethylene glycol with lycopene groups yielded to slight higher percentages of membrane integrity assessed by HOST than that of the other groups, but 7% glycerol with cysteamine and 3% ethylene glycol with cysteamine showed the worst percentages of membrane integrity. Glycerol 7% and 5% glycerol with lycopene gave rise to a higher value of VAP, VSL and VCL compared with that of the other groups. On the contrary, adding to 5% glycerol with cysteamine showed negative effect for VAP, VSL, VCL and ALH values. All cryoprotectant groups with lycopene decreased chromatin damage than the other groups. Ethylene glycol 3% led to lower non‐return rates of inseminated cows. However, this result was not considered to be statistically important.  相似文献   

Morphological and molecular characterization of garlic (Allium sativum L.) genotypes sampled from Turkey     

K&#;ra&#;  Hayrettin  Dalda &#;ekerci  Akife  Co&#;kun  &#;mer Faruk  G&#;l&#;en  Osman 《Genetic Resources and Crop Evolution》2022,69(5):1833-1841

Genetic Resources and Crop Evolution - Garlic is a vegetable widely used both in food and as a pharmaceutical raw material in the world due to its contents. Although morphological differences are...  相似文献   

Genetic variation in susceptibility of Atlantic salmon, Salmo salar L., to furunculosis, BKD and cold water vibriosis     

T Gjedrem  H M Gjøen 《Aquaculture Research》1995,26(2):129-134

Genetic variation in susceptibility of Atlantic salmon, Salmo salar L., to furunculosis, bacterial kidney disease (BKD) and cold water vibriosis was studied by challenge testing one-year-old fingerlings. Fish from 81 full-sib families within 32 sire progeny groups were infected with Aeromonas salmonicida, Renibacterium salmoninarum and Vibrio salmonicida. Estimated heritabilities were relatively low, being highest for BKD (h²= 0.23) and lowest for cold water vibriosis (h²= 0.13). Genetic correlations between the ability to survive the diseases were all positive, but the magnitude of the genetic correlation between furunculosis and BKD may be biased upwards because some of the dead BKD fish were also infected with furunculosis. The application of selection to develop resistant populations of Atlantic salmon is advocated. Challenge testing seems to be a feasible method, with relatively low costs and easy management. The future response to selection will depend on the relationships between results from a challenge test and mortalities under farming conditions and between disease resistance and other traits in the breeding goal.  相似文献   

A study of genetic diversity in bottle gourd [Lagenaria siceraria (Molina) Standl.] population,and implication for the historical origins on bottle gourds in Turkey     

Kahraman Gürcan  Ahmet Say  Halit Yetişir  Nihal Denli 《Genetic Resources and Crop Evolution》2015,62(3):321-333

  相似文献   

Mineral Composition of Leaves and Fruit in Some Myrtle (Myrtus communis L.) Genotypes     

F. Yildirim  B. Şan  A. N. Yildirim  M. Polat  S. Ercişli 《Erwerbs-Obstbau》2015,57(3):149-152

  相似文献   

Dirty Soil and Clean Consciences: Examining Communication of Contaminated Soil     

Tina Wiséen  Misse Wester-Herber 《Water, air, and soil pollution》2007,181(1-4):173-182

The identification and remediation of contaminated sites in Europe is a continuous undertaking that includes different aspects. There are many variables to take into consideration such as the nature of the contaminants, the risks they pose, the location of the site and possible future usages. Also, possible negative effects on the local residents or the environment have to be considered. Within this context, it is necessary to establish a communication between different actors, such as industry, authorities and municipalities, as well as with the surrounding public. This can be done in a variety of ways, where some are more useful and constructive than others. In the present study, eight different construction companies and municipalities were interviewed in order to elicit their views on and experiences of risk communication. The results show that even though most actors were seriously committed to involve and respond to the local populations’ concerns and fears, there is certainly room for improvement in many areas. Concluding remarks call for an increased exchange of experiences with all actors involved in risk research and to develop better official guidelines for communicating risks that are specific for contaminated soil.  相似文献   

Antimicrobial screening of Mentha piperita essential oils     

Işcan G  Kirimer N  Kürkcüoğlu M  Başer KH  Demirci F 《Journal of agricultural and food chemistry》2002,50(14):3943-3946

Essential oils of peppermint Mentha piperita L. (Lamiaceae), which are used in flavors, fragrances, and pharmaceuticals, were investigated for their antimicrobial properties against 21 human and plant pathogenic microorganisms. The bioactivity of the oils menthol and menthone was compared using the combination of in vitro techniques such as microdilution, agar diffusion, and bioautography. It was shown that all of the peppermint oils screened strongly inhibited plant pathogenic microorganisms, whereas human pathogens were only moderately inhibited. Chemical compositions of the oils were analyzed by GC and GC/MS. Using the bioautography assay, menthol was found to be responsible for the antimicrobial activity of these oils.  相似文献   

吸水链霉菌桂林新变种的分类研究     

夏湛恩  黄文彩  施伟良  姚杭丽  吴文娟 《浙江大学学报(农业与生命科学版)》1995,(3)

从广西桂林的土壤中分离到一株链霉菌（编号为２３４５），它的发酵产物对线虫、菜青虫、松毛虫等畜禽寄生虫及作物害虫等的防治效果显著，它对一些格兰氏阳性菌也有明显的抑制作用，对其进行分类学研究的结果如下：按照链霉菌的分类法，该菌细胞成份属于细胞壁Ｉ型，糖Ｃ型。该菌在高氏培养基、ＩＳＰ－４等培养基上产生丰富的孢子，在斜面上则呈现一片褐色斑。该菌在培养特征、碳源利用以及形态特征等方面均近似于淡紫灰吸水链霉菌，但对淀粉利用能力很强。另外，它对枯草杆菌、藤黄八叠球菌等阳性菌的抗菌作用强，尤其是它的发酵产物可以杀死禽畜体内的线虫，故属淡紫灰吸水链霉菌桂林变种Ｓｔｒｅｐｔｏｍｙｃｅｓｌａｖｅｎｄｕｌｏｈｙｇｒｏｓｃｏｐｉｃｕｓｖａｒ．ｇｕｉｌｉｎｎ．Ｘｉａ．  相似文献