Molecular biological studies of the pathogenicity of <Emphasis Type="Italic">Xanthomonas oryzae</Emphasis> pv. <Emphasis Type="Italic">oryzae</Emphasis>     

Ayako Furutani 《Journal of General Plant Pathology》2009,75(6):458-459

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Differences in stable isotope ratios of Dall’s porpoises (Phocoenoides dalli) between coastal and oceanic areas of the North Pacific     

HIROSHI OHIZUMI  NOBUYUKI MIYAZAKI 《Fisheries Oceanography》2010,19(4):257-261

Carbon and nitrogen stable isotope ratios in the muscle of Dall’s porpoises were measured. Samples were collected from the catches of the hand harpoon fishery, incidental catches of drift net, and scientific research on the use of drift nets. Samples were from the North Pacific, Sea of Japan, Sea of Okhotsk, and Bering Sea. Although no variation in δ¹⁵N was observed, δ¹³C was significantly different between population groups near Japan and the oceanic North Pacific and Bering Sea. The difference may be due not only to local variation in prey species, but also to an overall difference in carbon stable isotope ratios that originate from coastal benthic or oceanic pelagic‐based food webs. We differentiated Dall’s porpoise population groups from both areas using carbon stable isotope ratios with an error rate of <5%. Although further study is needed, our results suggest that carbon stable isotope ratios could possibly be an indicator of whether a Dall’s porpoise belongs to a coastal benthic or oceanic pelagic food web.  相似文献   

Rapid identification of Ochroconis gallopava by a loop-mediated isothermal amplification (LAMP) method     

Ohori A  Endo S  Sano A  Yokoyama K  Yarita K  Yamaguchi M  Kamei K  Miyaji M  Nishimura K 《Veterinary microbiology》2006,114(3-4):359-365

Ochroconis gallopava is a species of dematiaceous fungi recognized as a causative agent of zoonotic and emerging fungal infections. It affects the central nervous system and respiratory tracts of humans, birds and cats. We designed O. gallopava species-specific primer sets to aid in its identification by a loop-mediated isothermal amplification (LAMP) method based on the D1/D2 domain of the LSU rDNA sequence. The LAMP method successfully detected the gene from both fungal DNA and experimentally infected brains and spleens of mice and will be helpful in the diagnosis of O. gallopava infection.  相似文献   

Relationship between hybridization frequency of Brassica juncea × B. napus and distance from pollen source (B. napus) to recipient (B. juncea) under field conditions in Japan     

Mai Tsuda  Ayako Okuzaki  Yukio Kaneko  Yutaka Tabei 《Breeding Science》2012,62(3):274-281

Several imported transgenic canola (Brassica napus) seeds have been spilled and have grown along roadsides around import ports. B. juncea, a relative of B. napus with which it has high interspecific crossability, is widely distributed throughout Japan. There is public concern about the harmful impacts of feral B. napus plants on biodiversity, but spontaneous hybridization between spilled B. napus and weedy B. juncea populations is hardly revealed. We evaluated the relationship between the hybridization frequency of B. juncea × B. napus and their planting distance in field experiments using the mutagenic herbicide-tolerant B. napus cv. Bn0861 as a pollen source for hybrid screening. The recipient B. juncea cv. Kikarashina was planted in an experimental field with Bn0861 planted in the center. No hybrids were detected under natural flowering conditions in 2009. However, the flowering period was artificially kept overlapping in 2010, leading to a hybridization frequency of 1.62% in the mixed planting area. The hybridization frequency decreased drastically with distance from the pollen source, and was lower under field conditions than estimated from the high crossability, implying that spontaneous hybridization between spilled B. napus and weedy B. juncea is unlikely in the natural environment.  相似文献   

Persistent C genome chromosome regions identified by SSR analysis in backcross progenies between Brassica juncea and B. napus     

Mai Tsuda  Ayako Okuzaki  Yukio Kaneko  Yutaka Tabei 《Breeding Science》2012,62(4):328-333

Given that feral transgenic canola (Brassica napus) from spilled seeds has been found outside of farmer’s fields and that B. juncea is distributed worldwide, it is possible that introgression to B. juncea from B. napus has occurred. To investigate such introgression, we characterized the persistence of B. napus C genome chromosome (C-chromosome) regions in backcross progenies by B. napus C-chromosome specific simple sequence repeat (SSR) markers. We produced backcross progenies from B. juncea and F₁ hybrid of B. juncea × B. napus to evaluate persistence of C-chromosome region, and screened 83 markers from a set of reported C-chromosome specific SSR markers. Eighty-five percent of the SSR markers were deleted in the BC₁ obtained from B. juncea × F₁ hybrid, and this BC₁ exhibited a plant type like that of B. juncea. Most markers were deleted in BC₂ and BC₃ plants, with only two markers persisting in the BC₃. These results indicate a small possibility of persistence of C-chromosome regions in our backcross progenies. Knowledge about the persistence of B. napus C-chromosome regions in backcross progenies may contribute to shed light on gene introgression.  相似文献   

Deep granulomatous dermatitis of the fin caused by Fusarium solani in a false killer whale (Pseudorca crassidens)     

Tanaka M  Izawa T  Kuwamura M  Nakao T  Maezono Y  Ito S  Murata M  Murakami M  Sano A  Yamate J 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(6):779-782

A 10-year-old female false killer whale (Pseudorca crassidens) developed skin lesions in the left breast fin. Histopathologically, the lesions consisted of multiple granulomas spread diffusely into the deep dermis and bone; characteristically, each granuloma had septate, branching fungal hyphae and chlamydospores surrounded by eosinophilic Splendore-Hoeppli materials. Macrophages, epithelioid cells and multinucleated giant cells in the granulomas reacted mainly to anti-SRA-E5 antibody against human macrophage scavenger receptor type I. Fusarium solani was isolated and its gene was detected from the skin samples. Mycotic skin lesions by Fusarium spp. reported so far in marine mammals were regarded as superficial dermatitis; therefore, the present case is very uncommon in that the lesions spread deeper into the skin.  相似文献   

High-grade myofibroblastic sarcoma of inguinal region in a dog     

Hojo Y  Tsuchiya T  Shiraki A  Suzuki K  Shibutani M  Mitsumori K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(5):625-628

A subcutaneous tumor in the left inguinal region was present in an 11-year-old female bloodhound. Histopathologically, the tumor showed invasive growth and extensive necroses, and it was composed of spindle-shaped, elongated, and stellate neoplastic cells accompanied by occasional giant cells arranged in fascicular, herringbone, or irregular storiform patterns with abundant production of collagen fibers. The cytoplasm of most tumor cells was positive for vimentin, alpha-smooth muscle actin, and calponin, but was negative for desmin, smoothelin, and S-100. Furthermore, most of the tumor cells were negative for Iba1 while some tumor cells were weakly positive. Thus, this tumor was diagnosed as a high-grade myofibroblastic sarcoma according to the diagnostic criteria for human myofibroblastic sarcomas.  相似文献   

Feline demodicosis caused by an unnamed species     

Kano R  Hyuga A  Matsumoto J  Nogami S  Nemoto S  Hasegawa A  Kamata H 《Research in veterinary science》2012,92(2):257-258

A case of feline demodicosis is described in this report. A 13-year-old spayed female domestic short hair cat weighing 4.5 kg was being treated with cefovecin and alternately with prednisone or methylprednisolone. On further physical examination, the cat showed mild erythema and hair loss on the bridge of the nose, around the eyes, on the chin, on the side part of the breast and on the abdomen. A large number of Demodex mites were found in deep skin scrapings from the affected areas. The cat was then treated with ivermectin at 600 μg/kg administered SC daily. After 4 weeks of treatment, the cat was clinically normal with no mites detected in the skin scrapings from the face or breast areas. The mite responsible may represent a previously seen but as yet unnamed new species. This is third report that describes a case of feline demodicosis caused by a different, unnamed mite species that has different morphological characteristics to those of known Demodex mites and may represent a previously seen but as yet unnamed species.  相似文献   

Effect of durotomy in dogs with thoracolumbar disc herniation and without deep pain perception in the hind limbs     

Fumitaka Takahashi BVSc  PhD  Ayaka Honnami BVSc  Minae Toki BVSc  Ayako Dosaka BVSc  Yukihiro Fujita BVSc  MS  PhD  Yasushi Hara BVSc  PhD  Shinya Yamaguchi BVSc  PhD 《Veterinary surgery : VS》2020,49(5):860-869

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External transcutaneous ultrasound technique in the equine cricoarytenoideus dorsalis muscle: Assessment of muscle size and echogenicity with resting endoscopy     

Masato Satoh  Tohru Higuchi  Satoshi Inoue  Daisuke Miyakoshi  Ayako Kajihara  Tadahiro Gotoh  Yasuhito Shimizu 《Equine veterinary journal》2020,52(4):500-508

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