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排序方式: 共有455条查询结果,搜索用时 15 毫秒
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FJ Peña M Plaza Davila BA Ball EL Squires P Martin Muñoz C Ortega Ferrusola C Balao da Silva 《Reproduction in domestic animals》2015,50(4):529-537
The traditional assessment of stallion sperm comprises evaluation of sperm motility and membrane integrity and identification of abnormal morphology of the spermatozoa. More recently, the progressive introduction of flow cytometry is increasing the number of tests available. However, compared with other sperm structures and functions, the evaluation of mitochondria has received less attention in stallion andrology. Recent research indicates that sperm mitochondria are key structures in sperm function suffering major changes during biotechnological procedures such as cryopreservation. In this paper, mitochondrial structure and function will be reviewed in the stallion, when possible specific stallion studies will be discussed, and general findings on mammalian mitochondrial function will be argued when relevant. Especial emphasis will be put on their role as source of reactive oxygen species and in their role regulating sperm lifespan, a possible target to investigate with the aim to improve the quality of frozen–thawed stallion sperm. Later on, the impact of current sperm technologies, principally cryopreservation, on mitochondrial function will be discussed pointing out novel areas of research interest with high potential to improve current sperm technologies. 相似文献
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脑包净脑室内注射治疗羊脑包虫病效果观察 总被引:1,自引:0,他引:1
为了驱治羊脑包虫病,采用自制的“脑包净”注射液进行侧脑室和包囊内注射治疗。病羊41 只,治愈31 只,治愈率75.61% ;其中侧脑室注射的治愈率84.62% ,但需较长时间才痊愈;包囊内注射的治愈率71.43% ,但痊愈较快,较彻底。脑内注射给药治疗羊脑包虫病较手术疗法施治面宽,无复发隐患,安全性好,操作简便;较其它途径给药疗法用药量少,疗效高,副作用小 相似文献
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AIM: To determine the role of the glycocalyx in microvascular permeability. METHODS: Dextran was intravenously injected and quatitatively examined in the rat transient cerebral ischemic model. At the same time, endothelial glycocalyx(anionic sites) was labelled with the probe cationic gold colloid(CGC) using post-embedding technique and examined with electron microscope. RESULTS: The labeling of CGC decreased significantly following ischemia, meanwhile, microvascular permeability to dextran increased. CONCLUSION:Endothelial glycocalyx is very sensitive to ischemia or anoxia. Its disruption may be the initiator of the dysfunction of endothelium and the determinant of increased permeability. 相似文献
388.
J. A. ORSINI dvm ANGELINE WARNER dvm SUSAN DYSON BA VetMB DAVID NUNAMAKER VMD DiplomateACVS 《Veterinary surgery : VS》1985,14(4):307-309
During correction of a dystocia, a fracture occurred involving the right third and fourth metacarpus of a calf. Application of a plaster cast resulted in devitalization of the limb. The distal limb was amputated, and a prosthetic device capable of periodic lengthening was fitted. The calf was able to walk well 1 month following surgery. 相似文献
389.
MA Hayes BA Quinn ND Keirstead P Katavolos RO Waelchli KJ Betteridge 《Reproduction in domestic animals》2008,43(S2):232-237
A critical period of early gestation in the mare involves the immobilization (fixation) of the encapsulated conceptus at around days 16–17. We compared the major proteins in the normal equine embryonic capsule and endometrial secretions around the period of fixation with those from pregnancies in the process of termination induced by administration of an analogue of prostaglandin F2α (PGF2α ). Uterocalin and β2 -microglobulin (β2 M) associated with the embryonic capsule were proteolytically converted to smaller forms during the fixation period. These conversions were similar in conceptuses from control and treated mares. A 17 kDa cationic protein identified as a secretory phospholipase A2 (sPLA2) type IIA was detected bound to normal capsules but increased substantially in response to PGF2α . Two forms of uteroglobin were distinguished by partial amino acid sequences of ∼6 kDa bands in flush fluids from normal pregnant uteri. After administration of PGF2α one immunoreactive form of uteroglobin was preferentially increased. These studies demonstrate that failure of pregnancy in this model is associated with an increase in secretory phospholipase in the capsule and a change in the forms of uteroglobin in the uterine secretions. 相似文献
390.
小麦线粒体与细胞质雄性不育关系的研究进展 总被引:1,自引:0,他引:1
细胞质雄性不育(cytoplasmic male sterility,CMS)是小麦杂种优势利用的重要途径之一,大量研究表明CMS是线粒体基因与细胞核基因互作的结果。线粒体基因组是细胞质育性因子的载体,其突变和重组与CMS有直接关系。本文综述了线粒体基因组的结构特征及线粒体DNA多态性、线粒体基因转录、线粒体多肽差异和线粒体细胞学超微结构等几个方面与CMS的关系,并对小麦CMS分子机制进行探讨,以期为清晰揭示CMS形成的分子机制及最终对CMS三系杂交种选育和作物杂种优势利用提供参考。 相似文献