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LM Antunes MSc DVM JV Roughan BSc PhD & PA Flecknell MA Vet MB PhD DLAS Dip ECVA 《Veterinary anaesthesia and analgesia》2001,28(4):196-203
Objectives To assess a method for monitoring depth of anaesthesia using components of middle latency auditory evoked potential (AEP) waveforms during anaesthesia with fentanyl/fluanisone and midazolam. Study design Prospective observational study. Animals Five female Wistar rats weighing between 210 and 250 g. Methods Implanted electrodes were used to record AEPs in animals receiving five doses of anaesthetic. Recordings were made at 5 minutes post‐injection (deep anaesthesia; no pedal withdrawal response, PWR) and then at 25 minutes (light anaesthesia; strong PWR). Responses showed five characteristic peaks occurring at 11, 14, 23, 42 and 68 ms that were measured for latency of occurrence and peak amplitude. Results Auditory evoked potential peaks P14, N23 and P42 were increased significantly in latency with successive anaesthetic injections [avg. F(1,4) = 12.53, p < 0.001; avg. F(1,4) = 10.6, p < 0.001; avg. F(1,4) = 3.9, p = 0.02, respectively]. Peak N23 showed a significant reduction in latency during the 20 minute recovery period following both the first and second anaesthetic injections (t(3) = 7.52, p = 0.005; t(4) = 5.17, p = 0.007, respectively). Peak P42 occurred significantly earlier 20 minutes following the second anaesthetic injection (t(4) = 4.75, p = 0.009). The mean overall depth of anaesthesia assessed using PWR scores was significantly correlated with the mean latency of peak N23, such that as the strength of PWR increased, N23 occurred significantly earlier (r = ?0.99, p = 0.01). The amplitude difference between peaks N23 and P42 increased after the second and third drug administrations [avg. F(1,4) = 10.65, p = 0.031 and avg. F(1,4) = 11.24, p = 0.028, respectively]. Conclusion The characteristics of these peaks, and in particular latency of peak N23, may provide a useful tool for assessing depth of anaesthesia produced by this, and possibly other anaesthetic agents. 相似文献
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Victor JV Rossetto Lígia SLS da Mota Noeme S Rocha Hélio A Miot Fabrizio Grandi Cláudia VS Brand?o 《Acta veterinaria Scandinavica》2013,55(1):39
Background
Due to numerous complications associated to gastrointestinal augmented cystoplasty, this study aimed to analyze the anatomic repair of the bladder of 10 female dogs using grafts of porcine small intestinal submucosa (SIS) seeded with cultured homologous smooth muscle cells, and compare them with the acellular SIS grafts.Results
We assessed the possible side effects and complications of each type of graft by clinical examination, abdominal ultrasound and laboratory findings. Anatomic repair of neoformed bladder was assessed by histological staining for H/E and Masson''s Trichrome, analyzed with a Nikon Photomicroscope connected to the system of image analysis Image J.Conclusions
We propose that SIS associated to homologous smooth cells can improve the quality of tissue repair, and consequently decrease the potential complications inherent to acellular SIS. 相似文献17.
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NATHAN M. BACHELER LORENZO CIANNELLI KEVIN M. BAILEY JANET T. DUFFY‐ANDERSON 《Fisheries Oceanography》2010,19(2):107-120
Walleye pollock Theragra chalcogramma (pollock hereafter) is a key ecological and economic species in the eastern Bering Sea, yet detailed synthesis of the spatial and temporal patterns of pollock ichthyoplankton in this important region is lacking. This knowledge gap is particularly severe considering that egg and larval distribution are essential to reconstructing spawning locations and early life stages drift pathways. We used 19 yr of ichthyoplankton collections to determine the spatial and temporal patterns of egg and larval distribution. Generalized additive models (GAMs) identified two primary temporal pulses of pollock eggs, the first occurring from 20 February to 31 March and the second from 20 April to 20 May; larvae showed similar, but slightly lagged, pulses. Based on generalized cross‐validation and information theory, a GAM model that allowed for different seasonal patterns in egg density within three unique areas outperformed a GAM that assumed a single fixed seasonal pattern across the entire eastern Bering Sea. This ‘area‐dependent’ GAM predicted the highest densities of eggs (i.e., potential spawning locations) in three major areas of the eastern Bering Sea: near Bogoslof Island (February–April), north of Unimak Island and the Alaska Peninsula (March–April), and around the Pribilof Islands (April–August). Unique temporal patterns of egg density were observed for each area, suggesting that pollock spawning may be more spatially and temporally complex than previously assumed. Moreover, this work provides a valuable baseline of pollock spawning to which future changes, such as those resulting from climate variability, may be compared. 相似文献
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D. R. BAILEY 《Weed Research》1969,9(1):9-19
Summary. 2,4-D-amine, 2,4-D ethyl ester, 2.4-DB-potassium. MCPA-sodium, diquat and a 1:1 mixture of 2,4,5-T butyl ester and 2,4-D ethyl ester were applied to the tropical pasture legume Centrosema pubescens Benth. (centro) grown either alone or with Panicum maximum Jacq. (guinea grass). Even at 0.5 Ib/ac (total) the 2,4,5-T/2,4-D mixture caused severe damage to seedling and mature centro. 2,4-D-amine, 2,4-D ethyl ester and 2,4-DB-potassium were less damaging and there were only slight differences in response to these. Diquat at 0.25 Ib/ac desiccated centro seedlings, but most plants survived and grew satisfactorily. There was some evidence that the growth of mature centro was depressed least when the herbicides were applied during vegetative quiescence.
Application just after the start of flowering altered the flowering pattern but did not reduce seed yields significantly. 2,4-D-amine at 0.75 and 1–5 lb/ac and 2,4-D ethyl ester at 1.5 Ib/ac decreased the percentage germination of abraded seed. Diquat 0.25 Ib/ac applied at the start of flowering caused significant increases in top growth and seed pod numbers, possibly the result of modification of the normal flowering pattern. 相似文献
Application just after the start of flowering altered the flowering pattern but did not reduce seed yields significantly. 2,4-D-amine at 0.75 and 1–5 lb/ac and 2,4-D ethyl ester at 1.5 Ib/ac decreased the percentage germination of abraded seed. Diquat 0.25 Ib/ac applied at the start of flowering caused significant increases in top growth and seed pod numbers, possibly the result of modification of the normal flowering pattern. 相似文献