FITC: Something New or More of the Same?     

Denny BC 《Science (New York, N.Y.)》1979,203(4384):961

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Export-mediated assembly of mycobacterial glycoproteins parallels eukaryotic pathways     

VanderVen BC  Harder JD  Crick DC  Belisle JT 《Science (New York, N.Y.)》2005,309(5736):941-943

Protein O-mannosylation is an essential and evolutionarily conserved post-translational modification among eukaryotes. This form of protein modification is also described in Mycobacterium tuberculosis; however, the mechanism of mannoprotein assembly remains unclear. Evaluation of differentially translocated chimeric proteins and mass spectrometry to monitor glycosylation demonstrated that specific translocation processes were required for protein O-mannosylation in M. tuberculosis. Additionally, Rv1002c, a M. tuberculosis membrane protein homolog of eukaryotic protein mannosyltransferases, was shown to catalyze the initial step of protein mannosylation. Thus, the process of protein mannosylation is conserved between M. tuberculosis and eukaryotic organisms.  相似文献   

Climate change. Dangerous climate impacts and the Kyoto Protocol     

O'Neill BC  Oppenheimer M 《Science (New York, N.Y.)》2002,296(5575):1971-1972

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The high-technology fix     

Denny BC 《Science (New York, N.Y.)》1982,217(4562):781

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Direct measurement of the reaction front in chemically amplified photoresists     

Lin EK  Soles CL  Goldfarb DL  Trinque BC  Burns SD  Jones RL  Lenhart JL  Angelopoulos M  Willson CG  Satija SK  Wu WL 《Science (New York, N.Y.)》2002,297(5580):372-375

The continuing drive by the semiconductor industry to fabricate smaller structures using photolithography will soon require dimensional control at length scales comparable to the size of the polymeric molecules in the materials used to pattern them. The current technology, chemically amplified photoresists, uses a complex reaction-diffusion process to delineate patterned areas with high spatial resolution. However, nanometer-level control of this critical process is limited by the lack of direct measurements of the reaction front. We demonstrate the use of x-ray and neutron reflectometry as a general method to measure the spatial evolution of the reaction-diffusion process with nanometer resolution. Measuring compositional profiles, provided by deuterium-labeled reactant groups for neutron scattering contrast, we show that the reaction front within the material is broad rather than sharply defined and the compositional profile is altered during development. Measuring the density profile, we directly correlate the developed film structure with that of the reaction front.  相似文献   

Mariner 9 television reconnaissance of Mars and its satellites: preliminary results     

Masursky H  Batson RM  McCauley JF  Soderblom LA  Wildey RL  Carr MH  Milton DJ  Wilhelms DE  Smith BA  Kirby TB  Robinson JC  Leovy CB  Briggs GA  Duxbury TC  Acton CH  Murray BC  Cutts JA  Sharp RP  Smith S  Leighton RB  Sagan C  Veverka J  Noland M  Lederberg J  Levinthal E  Pollack JB  Moore JT  Hartmann WK  Shipley EN  De Vaucouleurs G  Davies ME 《Science (New York, N.Y.)》1972,175(4019):294-305

At orbit insertion on 14 November 1971 the Martian surface was largely obscured by a dust haze with an extinction optical depth that ranged from near unity in the south polar region to probably greater than 2 over most of the planet. The only features clearly visible were the south polar cap, one dark, spot in Nix Olympica, and three dark spots in the Tharsis region. During the third week the atmosphere began to clear and surface visibility improved, but contrasts remained a fraction of their normal value. Each of the dark spots that apparently protrude through most of the dust-filled atmosphere has a crater or crater complex in its center. The craters are rimless and have featureless floors that, in the crater complexes, are at different levels. The largest crater within the southernmost spot is approximately 100 kilometers wide. The craters apparently were formed by subsidence and resemble terrestrial calderas. The south polar cap has a regular margin, suggsting very flat topography. Two craters outside the cap have frost on their floors; an apparent crater rim within the cap is frost free, indicating preferentia loss of frost from elevated ground. If this is so then the curvilinear streaks, which were frost covered in 1969 and are now clear of frost, may be low-relief ridges. Closeup pictures of Phobos and Deimos show that Phobos is about 25 +/-5 by 21 +/-1 kilometers and Deimos is about 13.5 +/- 2 by 12.0 +/-0.5 kilometers. Both have irregular shapes and are highly cratered, with some craters showing raised rims. The satellites are dark objects with geometric albedos of 0.05.  相似文献   

Pyroclastic activity at Home Plate in Gusev Crater, Mars     

Squyres SW  Aharonson O  Clark BC  Cohen BA  Crumpler L  de Souza PA  Farrand WH  Gellert R  Grant J  Grotzinger JP  Haldemann AF  Johnson JR  Klingelhöfer G  Lewis KW  Li R  McCoy T  McEwen AS  McSween HY  Ming DW  Moore JM  Morris RV  Parker TJ  Rice JW  Ruff S  Schmidt M  Schröder C  Soderblom LA  Yen A 《Science (New York, N.Y.)》2007,316(5825):738-742

Home Plate is a layered plateau in Gusev crater on Mars. It is composed of clastic rocks of moderately altered alkali basalt composition, enriched in some highly volatile elements. A coarsegrained lower unit lies under a finer-grained upper unit. Textural observations indicate that the lower strata were emplaced in an explosive event, and geochemical considerations favor an explosive volcanic origin over an impact origin. The lower unit likely represents accumulation of pyroclastic materials, whereas the upper unit may represent eolian reworking of the same pyroclastic materials.  相似文献   

Differences between tight and loose cultures: a 33-nation study     

Gelfand MJ  Raver JL  Nishii L  Leslie LM  Lun J  Lim BC  Duan L  Almaliach A  Ang S  Arnadottir J  Aycan Z  Boehnke K  Boski P  Cabecinhas R  Chan D  Chhokar J  D'Amato A  Ferrer M  Fischlmayr IC  Fischer R  Fülöp M  Georgas J  Kashima ES  Kashima Y  Kim K  Lempereur A  Marquez P  Othman R  Overlaet B  Panagiotopoulou P  Peltzer K  Perez-Florizno LR  Ponomarenko L  Realo A  Schei V  Schmitt M  Smith PB  Soomro N  Szabo E  Taveesin N  Toyama M  Van de Vliert E  Vohra N  Ward C  Yamaguchi S 《Science (New York, N.Y.)》2011,332(6033):1100-1104

With data from 33 nations, we illustrate the differences between cultures that are tight (have many strong norms and a low tolerance of deviant behavior) versus loose (have weak social norms and a high tolerance of deviant behavior). Tightness-looseness is part of a complex, loosely integrated multilevel system that comprises distal ecological and historical threats (e.g., high population density, resource scarcity, a history of territorial conflict, and disease and environmental threats), broad versus narrow socialization in societal institutions (e.g., autocracy, media regulations), the strength of everyday recurring situations, and micro-level psychological affordances (e.g., prevention self-guides, high regulatory strength, need for structure). This research advances knowledge that can foster cross-cultural understanding in a world of increasing global interdependence and has implications for modeling cultural change.  相似文献   

The bonding electron density in aluminum     

Nakashima PN  Smith AE  Etheridge J  Muddle BC 《Science (New York, N.Y.)》2011,331(6024):1583-1586

Aluminum is considered to approach an "ideal" metal or free electron gas. The valence electrons move freely, as if unaffected by the presence of the metal ions. Therefore, the electron redistribution due to chemical bonding is subtle and has proven extremely difficult to determine. Experimental measurements and ab initio calculations have yielded substantially different results. We applied quantitative convergent-beam electron diffraction to aluminum to provide an experimental determination of the bonding electron distribution. Calculation of the electron distribution based on density functional theory is shown to be in close agreement. Our results yield an accurate quantitative correlation between the anisotropic elastic properties of aluminum and the bonding electron and electrostatic potential distributions.  相似文献   

Kinetic isotope effects for the reactions of muonic helium and muonium with H2     

Fleming DG  Arseneau DJ  Sukhorukov O  Brewer JH  Mielke SL  Schatz GC  Garrett BC  Peterson KA  Truhlar DG 《Science (New York, N.Y.)》2011,331(6016):448-450

The neutral muonic helium atom may be regarded as the heaviest isotope of the hydrogen atom, with a mass of ~4.1 atomic mass units ((4.1)H), because the negative muon almost perfectly screens one proton charge. We report the reaction rate of (4.1)H with (1)H(2) to produce (4.1)H(1)H + (1)H at 295 to 500 kelvin. The experimental rate constants are compared with the predictions of accurate quantum-mechanical dynamics calculations carried out on an accurate Born-Huang potential energy surface and with previously measured rate constants of (0.11)H (where (0.11)H is shorthand for muonium). Kinetic isotope effects can be compared for the unprecedentedly large mass ratio of 36. The agreement with accurate quantum dynamics is quantitative at 500 kelvin, and variational transition-state theory is used to interpret the extremely low (large inverse) kinetic isotope effects in the 10(-4) to 10(-2) range.  相似文献