Attempts were made to isolate Neospora caninum from naturally infected white-tailed deer (Odocoileus virginianus). A total of 110 deer killed during the 2003 hunting season in Virginia region were used for the isolation of N. caninum. Of these, brains from 28 deer that had NAT titer of 1:200 were inoculated into interferon-gamma gene knock out (KO) mice. N. caninum was isolated from the tissues of three deer and all three isolates were mildly virulent to KO mice. Only one of the isolates could be adapted to in vitro growth. Protozoa in the tissues of KO mice reacted with N. caninum-specific polyclonal antibodies and N. caninum DNA was demonstrated in infected tissues by PCR assays; sequences of portions of the ITS-1 and gene 5 loci were identical to those in the public database. This is the first record of in vitro isolation of N. caninum from white-tailed deer and lends credence to the white-tailed deer as an intermediate host for this parasite. 相似文献
The objective in the present experiment was to study the effects of melengestrol acetate (0.5 mg for 14 days starting at day 15 of the estrous cycle) on the cytophysiologic character of the bovine adenohypophysis. Twelve Hereford and Angus heifers were used. A combination of thick-thin sectioning of resin-embedded material was used to aid in specific identification of individual hypophyseal cells. Alterations were not observed in the cytophysiologic activity of the presumptive prolactin-, somatotropin-, thyrotropin- or corticotropin-secreting cells. A marked increase in chromophobes was observed in animals given melengestrol acetate. Evidence indicated that these chromophobes originated from gonadotropin-secreting cells. 相似文献
The total hexosamine, or glucosamine and galactosamine contents and hydroxy‐proline content of secretory tissue of the isthmus and uterus were determined.
A low‐Mn diet depressed the glucosamine content of isthmus and uterus but had no effect on galactosamine nor hydroxyproline.
The contents of hexosamine were similar after 3 months of egg production to those at the start of lay, and hexosamine and hydroxyproline values did not differ whether a non‐calcified egg was in the isthmus or one with an almost fully formed shell was in the uterus.
The glucosamine content of the isthmus was significantly greater than that of the uterus; the reverse was found for hydroxyproline and there was no difference between the galactosamine contents. In tissue from both regions the concentrations of glucosamine was several times greater than that of galactosamine. 相似文献
The movement of radiopaque media in the ureters, cloaca, colon and caeca was studied radiographically in 12 in‐lay light hybrid hens. During direct screening of the conscious birds after intravenous administration of sodium diacetamidotriiodobenzoate, the contrast medium was observed to pass along the ureters to their distal extremity under the action of ureteral contractions. It accumulated in the coprodeum, which remained quiescent in some birds, but in others, periods of vigorous contraction of the coprodeum forced the material cranially into the colon. Retroperistaltic contractions of the colon which appeared to originate in the coprodeum were then seen to promote further retrograde movement and on several occasions the contrast medium entered the caeca. Retrograde movement of a suspension of barium sulphate from the coprodeum into the colon and caeca was also observed, but neither of the contrast media entered the ileum. Post‐mortem radiographs of the viscera confirmed the presence of the contrast media in the cloaca, colon and caeca. The findings are illustrated by radiographs and the contribution of retrograde flow to caecal filling is discussed. 相似文献
Summary Cattle in a communal farming area of Zimbabwe were identified and examined in the late dry and mid wet season of 1985/86.
Ovarian activity rates were 17·9% and 38·6% in the dry and wet season respectively. Pregnancy rates were 16·1% and 24·6% respectively.
A major limitation to reproduction was the percentage of anoestrous females. There was no evidence of a seasonal calving pattern.
Median body condition score was 2·0 in the dry season and 1·5 in the wet season. Adult females of body condition score 2·5
and above had ovarian activity rates of 41·9% and pregnancy rates of 41·9 per cent. Those with a body condition score below
2·5 had rates of 26·8% and 16·4% respectively. Serum progesterone levels in pregnant animals were positively related to body
condition score, with some animals having progesterone levels similar to non-pregnant animals. Metabolic profiles suggested
that the major limiting nutrient in both seasons was nitrogen, although other micronutrients might also be involved in low
reproductive rates.
Resumen Se identificó un grupo de ganado en un área comunal pastoril en Zimbabwe, el que se estudió al final de la estación seca y
en la mitad de la estación lluviosa. El estudio se llevó a cabo de 1985 a 1986. La tasas de actividad ovárica fueron de 17·9%
y 38·6% y las de pre?ez de 16·1% y 24·6%, en la estación seca y lluviosa, respectivamente. La mayor limitación para la reproducción,
fue el porcentaje alto de hembras en anestro. No hubo un patrón claro de partos estacionales. La condición corporal media
fue de 2·0 en la estación seca y de 1·5 en la estación lluviosa. Las hembras adultas que tenian una condición corporal de
2·5 ó mayor, tuvieron tasas de actividad ovárica de 41·9% y tasas de pre?ez de 41·9 por vaca. Aquellas con una condición corporal
por debajo de 2·5, tuvieron tasas de actividad ovárica de 26·8% y tasas de pre?ez de 16·4%, respectivamente. Los niveles de
progesterona en animales pre?ados, estuvieron positivamente relacionados con el puntaje obtenido en el análisis de la condición
corporal, aunque algunos animales tuvieron niveles de progesterona bajos, como de animales no pre?ados. Los perfiles matabólicos
revelaron, que el nutriente más deficitario fue el nitrógeno, aunque otros nutrientes tambien fueron deficitarios.
Résumé Dans une zone agricole de type communautaire, le bétail a été identifié et examiné à la fin de la saison humide et jusqu’au
milieu de la saison sèche en 1985/1986. Les taux d’activité ovarienne étaient respectivement de 17,9 et 38,6 p. 100 au cours
de la saison sèche et de la saison humide. Les taux de gestation étaient de 16,1 et 24,6 p. 100 respectivement. Un obstacle
essentiel à la reproduction s’est révélé être le nombre de femmelles en anoestrus. Aucune preuve n’a pu être apportée d’un
mode de mise bas lié à la saison. L’indice de condition corporelle moyenne mesuré à l’aide d’une échelle de points était de
2,0 en saison sèche et de 1,5 en saison humide. Les femelles adultes dont la condition corporelle était de 2,5 et au dessus,
avaient des taux d’activité ovarienne de 41,9 p. 100 et des taux de gestation par co?t identiques. Celles dont l’indice était
inférieur à 2,5 avaient des taux de 26,8 et de 16,4 p. 100 respectivement. Les taux de progestérone sérique chez les animaux
en gestation étaient positivement liés à l’indice de condition corporelle, avec quelques sujets dont le taux de progestérone
était identique à celui d’animaux non-gestants. Les profils métaboliques font penser que le facteur alimentaire limitant majeur
pour les deux saisons était l’azote, quoique d’autres micronutriments aient pu être également en cause dans les taux de reproduction
bas.
Four-week-old pigs of the Chester White, Duroc, Hampshire, Landrace, and Yorkshire breeds (n = 518) were vaccinated with a pseudorabies modified live-virus vaccine to determine whether genetic differences existed for immune response after vaccination. All pigs and their dams (sows) were tested before vaccination to determine preimmunization antibody titers, using a microtitration serum-neutralization (SN) test. The SN test results of sows were negative, as were preimmunization tests of the pigs. At 4 weeks after pigs were vaccinated, additional blood samples were collected from the pigs, and end-point SN titers were determined, using a 2-fold dilution scheme. Small, but statistically significant, breed differences existed for antibody response, with Yorkshire and Chester White pigs having the highest response, and Duroc and Landrace pigs, the lowest. Differences among sire progeny groups were small, but there were significant differences among dams. Genetic differences as seen by differences among breeds indicates that the efficacy of vaccines may vary from breed to breed and that vaccine trials should not neglect this potential source of variation. 相似文献