Strain variation in Dermatophilus congolensis demonstrated by cross-protection studies     

T M Ellis  S S Sutherland  G Davies 《Veterinary microbiology》1991,28(4):377-383

Cross-protection studies were conducted with vaccines prepared from two isolates of Dermatophilus congolensis (designated strain 1 and strain 2). The vaccines were prepared as either heat-inactivated, washed, formalized filamentous phase bacterium, mixed with alum as an adjuvant, and inoculated intramuscularly (type A vaccine) or sedimented live filaments inoculated intradermally (type B vaccine). The vaccinated sheep were challenged with D. congolensis zoospores of one or other strain. Challenge sites were observed for the presence and severity of lesions. Serum antibody levels to D. congolensis were monitored after vaccination and challenge. Type A and B vaccines from both strains produced some reduction in the severity of lesions when sheep were challenged with strain 1 but not with strain 2. Unvaccinated control sheep developed more severe and persistent lesions when challenged with strain 2 than controls challenged with strain 1. Serum antibody levels to the type B vaccine prepared from strain 1 were significantly higher (P less than 0.05) than antibody levels to type B vaccine from strain 2. These findings showed there was significant variation in virulence and antigenicity between these two isolates of D. congolensis.  相似文献   

Development of an enzyme-linked immunosorbent assay for the detection and measurement of the trypanocidal drug isometamidium chloride in cattle     

D D Whitelaw  E A Gault  P H Holmes  I A Sutherland  F J Rowell  A Phillips  G M Urquhart 《Research in veterinary science》1991,50(2):185-189

An enzyme-linked immunosorbent assay (ELISA) was developed to measure accurately levels of the trypanocidal drug isometamidium in the serum of treated cattle. The assay requires only 5 microliters of test serum, is sensitive to a level of 0.5 pg ml-1 and is highly specific. Cross reactivity does not occur with the two other widely used trypanocidal drugs diminazene aceturate and homidium bromide. Serum drug levels are detectable for up to six months in cattle after a single dose of 1 mg kg-1 intramuscularly, the maximum period under field conditions for which effective prophylaxis can be maintained against tsetse challenge. Application of the assay will aid the rationalisation of treatment campaigns and assist in assessing the occurrence of drug-resistant trypanosome populations.  相似文献   

Letter: Elaphostrongylus cervi in red deer.     

P C Mason  N R Kiddey  R J Sutherland  D M Rutherford  A G Green 《New Zealand veterinary journal》1976,24(1-2):22-23

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Antigenic relationship of the picorna-like virus of larval barramundi, Lates calcarifer Bloch to the nodavirus of larval striped jack, Pseudocaranx dentex (Bloch & Schneider)     

BL MUNDAY  T NAKAI  HD NGUYEN 《Australian veterinary journal》1994,71(11):384-385

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A census of the prevalence of vaginal prolapse in sheep flocks in the Borders region of Scotland     

J C Low  H K Sutherland 《The Veterinary record》1987,120(24):571-575

A postal census of vaginal prolapse in sheep flocks in the Borders region of Scotland yielded 540 replies from 963 owners (56 per cent). There were 262,250 ewes in 976 flocks and 2573 vaginal prolapses were reported. Analysis of the data revealed that 390 (40 per cent) of the flocks had no vaginal prolapses and in 237 (24.3 per cent) the reported prevalence was between 0.1 per cent and 1.0 per cent. Only 63 (6.5 per cent) of flocks had a greater than 5 per cent prevalence of vaginal prolapses. The greatest number of prolapses occurred in an upland flock of greyface ewes mated with Suffolk tups with 50 cases among 700 ewes (7.1 per cent) and the highest prevalence was in an upland Scottish blackface flock of ewes bred with Suffolk tups with 15.2 per cent (35 cases among 230 ewes). There were marked breed differences; very few hill breeds were affected and most cases occurred in greyface ewes mated with Suffolk tups.  相似文献   

AN OUTBREAK OF BOVINE TRICHOMONIASIS     

A. K. Sutherland  B.V.Sc.  M.S.  G. C. Simmonds  B.Sc. A. T. Bell  B.V.Sc. 《Australian veterinary journal》1953,29(3):67-69

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Body composition of mice selected for rapid growth rate   总被引：2，自引：0，他引：2  

P E Biondini  T M Sutherland  L H Haverland 《Journal of animal science》1968,27(1):5-12

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Serological response of cattle after vaccination and challenge with Brucella abortus     

S.S. Sutherland  D.V. Le Cras  A.G. Robertson  J.M. Johnston  R.J. Evans 《Veterinary microbiology》1982,7(2):165-175

New and currently used serological procedures were evaluated using sera from cattle that were challenged with B. abortus S544 (S544) after vaccination with either B. abortus S19 (S19) or B. abortus 45/20 (S45/20) as calves or adults. In animals vaccinated with S19, titres to the indirect haemolysis test (IHLT) rose more slowly, declined more rapidly and involved fewer animals than did titres to the complement fixation test (CFT). In animals vaccinated with S45/20 the rough antigen complement fixation test (RCFT) showed persistent titres. At slaughter the IHLT and CFT were found to be more specific and more sensitive than the Rose Bengal Plate Test (RBPT) and Serum Agglutination Test (SAT) in the detection of cattle infected with B. abortus.  相似文献   

PARENTERAL COPPER THERAPY IN RUMINANTS     

J. M.  Harvey  M.Sc. A. K.  Sutherland  B.V.Sc.  M.S. 《Australian veterinary journal》1953,29(10):261-268

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Preparation, assessment and preservation of antigen from Brucella abortus strain 45/20 for use in the rough antigen complement fixation test     

D V Cousins  S S Sutherland 《Veterinary microbiology》1984,10(1):87-90

Two methods of extraction were used to prepare antigens from Brucella abortus rough strain 45/20. The antigens were assessed for use in the complement fixation test. A suitable antigen was prepared using the saline extraction method of Miller et al. (1976) and used extensively in CF tests. Four methods of preservation were compared; -20 degrees C, -196 degrees C, 0.5% phenol at 4 degrees C, and lyophilisation. The antigen could be stored at -20 degrees C or -196 degrees C for up to 2 years.  相似文献