全文获取类型
收费全文 | 368篇 |
免费 | 37篇 |
专业分类
10篇 | |
综合类 | 6篇 |
农作物 | 1篇 |
水产渔业 | 1篇 |
畜牧兽医 | 386篇 |
植物保护 | 1篇 |
出版年
2023年 | 8篇 |
2020年 | 18篇 |
2018年 | 13篇 |
2017年 | 12篇 |
2016年 | 5篇 |
2015年 | 7篇 |
2014年 | 17篇 |
2013年 | 15篇 |
2012年 | 14篇 |
2011年 | 10篇 |
2010年 | 20篇 |
2009年 | 19篇 |
2008年 | 19篇 |
2007年 | 7篇 |
2006年 | 8篇 |
2005年 | 8篇 |
2004年 | 8篇 |
2003年 | 8篇 |
2002年 | 10篇 |
2001年 | 6篇 |
2000年 | 7篇 |
1999年 | 5篇 |
1998年 | 16篇 |
1997年 | 12篇 |
1996年 | 14篇 |
1995年 | 9篇 |
1994年 | 5篇 |
1993年 | 11篇 |
1992年 | 11篇 |
1991年 | 15篇 |
1990年 | 16篇 |
1989年 | 16篇 |
1988年 | 8篇 |
1987年 | 8篇 |
1986年 | 3篇 |
1985年 | 3篇 |
1984年 | 3篇 |
1983年 | 7篇 |
1982年 | 2篇 |
1975年 | 1篇 |
1968年 | 1篇 |
排序方式: 共有405条查询结果,搜索用时 31 毫秒
401.
Zachary J. Goodrich VMD Bo Norby CMV MPVM PhD Bunita M. Eichelberger DVM MS Diplomate ACVR Wade O. Friedeck BS Hollye N. Callis BS Don A. Hulse DVM Diplomate ACVS Sharon C. Kerwin DVM MS Diplomate ACVS Derek B. Fox DVM PhD Diplomate ACVS W. Brian Saunders DVM PhD Diplomate ACVS 《Veterinary surgery : VS》2014,43(7):791-803
402.
403.
404.
405.
Single layer centrifugation of fresh dromedary camel semen improves sperm quality and in vitro fertilization capacity compared with simple sperm washing
下载免费PDF全文
![点击此处可从《Reproduction in domestic animals》网站下载免费的PDF全文](/ch/ext_images/free.gif)
C Malo EG Crichton JM Morrell BS Pukazhenthi JA Skidmore 《Reproduction in domestic animals》2017,52(6):1097-1103
Single layer centrifugation (SLC) through a colloid is a tool for selecting viable mammalian spermatozoa but has not been used previously for fresh dromedary camel sperm. Semen from six camels (2 ejaculates/male) was diluted 1:5 (v:v) or 1:10 (v:v) in a Tris–citrate–fructose buffer for mechanical liquefaction by gentle pipetting. Following liquefaction, semen was processed either by SLC or by centrifugation without a colloid (control). Total and progressive motilities, CASA kinematics, vitality and acrosome integrity (eosin–nigrosin) and plasma membrane integrity (Hypo‐osmotic swelling test; HOST), and fertilizing ability in a heterologous assay (zona‐free goat oocytes) were evaluated. Both total (p = .003) and progressive motilities (p = .003) were higher in SLC‐processed than in control semen samples, irrespective of dilution. Positive HOST values increased when using colloid in 1:5 (p = .001) and 1:10 dilution (p = .010). Colloid‐selected sperm had higher penetration rates than controls (p < .001 and p = .02 for 1:5 and 1:10 dilutions, respectively). However, only the SLC sperm at 1:5 dilution showed higher percentages of pronuclear formation (p = .02) than controls. Dilution effect was only significant for total motility before in vitro fertilization, with higher values for the 1:5 dilution (p = .033). The recovery rates of motile sperm between dilutions were similar (26.1% vs 35.4%; p = .226). In conclusion, SLC is a promising tool for selecting functional dromedary camel sperm and warrants more research. 相似文献