Ultrasonographic features and prevalence of presumed gastric wall edema in dogs with hypoalbuminemia     

Masahiro Murakami  Hock Gan Heng  Chee Kin Lim  Nolie K. Parnell  Mario Sola 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2020,34(5):1867-1871

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Echocardiographic predictors of first onset of atrial fibrillation in dogs with myxomatous mitral valve disease     

Marco Baron Toaldo  Chiara Mazzoldi  Giovanni Romito  Helen Poser  Barbara Contiero  Mario Cipone  Carlo Guglielmini 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2020,34(5):1787-1793

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Use of oxytocin to attain cervical dilation for transcervical embryo transfer in sheep     

Vanessa M. B. dos Santos  Pedro Henrique N. Pinto  Mario Felipe A. Balaro  Juliana D. R. Santos  Augusto R. Taira  Caroline G. do Espirito Santo  Fernanda M. Gonçalves  Jeferson F. da Fonseca  Felipe Z. Brandão 《Reproduction in domestic animals》2020,55(10):1446-1454

The aim of this work was to determine whether a cervical dilation protocol (CDP) composed of only oxytocin can be used to perform transcervical (non-surgical) embryo transfer in sheep (NSET) without affecting the viability of the corpus luteum (CL). Likewise, we evaluated whether a cervical transposing test with a Hegar dilator (CT Hegar test), performed at oestrous time, could be used to screen ewes for NSET (greater or lower chances to transpose the cervix). For that, oestrous and ovulation synchronization was performed in 25 Santa Inês ewes to induce the dioestrous condition. Animals went through the following CDP in a crossover design: E + OX, oestradiol benzoate (100 µg intravenously [IV]) and oxytocin (100 IU IV); OX, oxytocin (100 IU IV); and SAL, saline solution (IV). Using a Hegar dilator, cervical transposing attempts were performed at oestrous (D0) and dioestrous time (D8). The viability of the CL (morphology, luteal blood flow and progesterone values) was evaluated by ultrasonography (colour Doppler and B-mode) and by serum progesterone measurement from D7 to D13. The cervical transposing rate was lower for the SAL group (64%; 16/25; p < .05) and did not differ between the E + OX (88%; 22/25, p > .05) and OX (84%; 21/25, p > .05) groups. No treatment affected the CL viability. The CT Hegar test showed a high sensitivity (85.7%–93.3%), satisfactory accuracy (72%–84%), low false-negative rate (6.7%–14.6%), but high false-positive rate (46%–66.7%). In conclusion, a CDP protocol composed exclusively of oxytocin can lead to good cervical transposing rates and does not affect the viability of the CL. In addition, a screening test (CT Hegar) performed at oestrus can identify ewes for which cervical transposing will likely not occur at NSET.  相似文献   

Using flower strips to promote green lacewings to control cabbage insect pests     

Alcalá Herrera  Rafael  Cotes  Belén  Agustí  Nuria  Tasin  Marco  Porcel  Mario 《Journal of pest science》2022,95(2):669-683

Habitat management improves biological control by increasing the abundance and fitness of natural enemies through the provision of floral resources along field edges or between crops. Among the natural enemies reliant on flower resources, green lacewings often stand out due to their abundance, predation capacity and polyphagy. We evaluated the impact of tailored flower strips on the enhancement of natural enemies, especially green lacewings, in three organic cabbage (Brassica oleracea) farms in Southern Sweden. Insects were sampled from the flower strips, and cabbage pests and predators were visually recorded in the crop. In a laboratory assay, the pollen feeding preferences of Chrysoperla carnea (Stephens, 1836) were evaluated in a dual-choice test. The pollen consumed by the Chrysopidae was extracted from laboratory and field specimens, then quantified and identified. Flower strips were found to attract predators and parasitoids, whose abundance increased as flowers bloomed. Cabbage plants next to the flower strips showed lower pest infestation as compared to cabbage plant control, although no significant differences were observed in the number of predators. Chrysopidae used flower strips as feeding, reproduction and shelter sites and mainly consumed pollen from Phacelia tanacetifolia Benth. Under laboratory conditions, C. carnea showed a preference for P. tanacetifolia and Coriandrum sativum L. pollen over Borago officinalis L. and Fagopyrum esculentum Moench. We show that tailored flower strips could be an efficient tool for enhancing beneficial arthropods and should be considered in integrated pest management for cabbage crops.

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A clinical retrospective study of Caparinia tripilis (Psoroptidae) mite dermatitis in pet African pygmy hedgehogs (Ateletrix albiventris) in southern Italy     

Dario d’Ovidio  Mario Santoro  Domenico Santoro 《Veterinary dermatology》2021,32(5):434-e115

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Conservation and use of genetic resources of cacao (Theobroma cacao L.) by gene banks and nurseries in six Latin American countries     

Ceccarelli  Viviana  Lastra  Sphyros  Loor Sol&#;rzano  Rey Gast&#;n  Chac&#;n  Walter Wenceslao  Nolasco  Mario  Sotomayor Cantos  Ignacio Antonio  Plaza Avell&#;n  Luis Fernando  L&#;pez  Diana Aracelly  Fern&#;ndez Anchundia  Fabian Marcelo  Dessauw  Dominique  Orozco-Aguilar  Luis  Thomas  Evert 《Genetic Resources and Crop Evolution》2022,69(3):1283-1302

Genetic Resources and Crop Evolution - Cacao (Theobroma cacao L.) is among the most important cash crops in tropical countries. The existing cacao genetic diversity represents a key resource to...  相似文献   

Use of Artificial Zeolites to Reduce Copper Toxicity to Two Marine Microalgae   总被引：1，自引：0，他引：1  

Herlinda Gómez  Villa Domenico  Voltolina Mario  Nieves Pablo  Pínta Jose Lopez  Ruiz 《Journal of the World Aquaculture Society》2002,33(2):214-219

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Bovine mammary epithelial cells retain stem-like phenotype in long-term cultures     

Cravero Diego  Martignani EugenioMiretti Silvia  Macchi ElisabettaAccornero Paolo  Baratta Mario 《Research in veterinary science》2014

The detection and characterization of bovine mammary stem cells may give a better understanding of the cyclic characteristic of mammary gland development. In turn, this could potentially offer techniques to manipulate lactation yield and for regenerative medicine. We previously demonstrated that adult stem cells reside in the bovine mammary gland and possess an intrinsic regenerative potential. In vitro maintenance and expansion of this primitive population is a challenging task that could make easier the study of adult mammary stem cells. The aim of this study is to investigate this possibility. Different subpopulations of mammary epithelial cells emerge when they are cultured in two defined culture conditions. Specific cell differentiation markers as cytokeratin 18 (CK18) and cytokeratin 14 (CK14) were expressed with significant differences according to culture conditions. Vimentin, a well-known fibroblast marker was observed to increase significantly (P < 0.5) only after day 20. In both conditions, after prolonged culture (25 days) a subset of cells still retained regenerative capabilities. These cells were able to form organized pseudo-alveoli when transplanted in immunodeficient mice as shown by the expression of cytokeratin 14 (CK14), cytokeratin 18 (CK18), p63 (a mammary basal cell layer marker) and Epithelial Cell Adhesion Molecule (EpCAM). We also were able to observe the presence of milk proteins signal in these regenerated structures, which is a specific marker of functional mammary alveoli. Progenitor content was also analyzed in vitro through Colony-Forming Cell (CFC) assays with no substantial differences among culture conditions and time points. These results demonstrate that long-term culture of a multipotent cell subpopulation with intrinsic regenerative potential is possible.  相似文献   

Calcium potentiates the effect of estradiol on PGF2α production in the bovine endometrium     

Claudia Maria Bertan Membrive  Pauline Martins da Cunha  Flávio Vieira Meirelles  Mario Binelli 《畜牧与生物技术杂志(英文版)》2014,5(1):25

Background

Estradiol (E₂) is required for luteolysis in cows and its injection stimulates prostaglandin F2α (PGF2α) release. The main goal of our study was to investigate the ability of endometrial explants and cells treated with E₂ and the calcium ionophore (CI) A23187 to synthesize PGF2α.

Results

Treatment with E₂in vivo resulted in a 48.4% increase of PGF2α production by endometrial explants treated in vitro with A23187. Production of PGF2α was better stimulated with A23187 at concentrations of 10^-6 and 10^-5 mol/L compared with other concentrations used. The concentration of PGF2α for untreated bovine endometrial cell cultures was 33.1 pg/mL, while for cultures treated with E₂, A23187, or a combination of E₂ and A23187, the PGF2α concentration was 32.5, 92.4 and 145.6 pg/mL, respectively.

Conclusions

Treatment with A23187 tended to stimulate PGF2α production. In the presence of E2, A23187 significantly stimulated PGF2α synthesis. It appears that A23187 potentiates the effects of E₂ with respect to synthesis of endometrial PGF2α in cattle.  相似文献   

MULTIDETECTOR ROW COMPUTED TOMOGRAPHY AND ULTRASOUND CHARACTERISTICS OF CAUDAL VENA CAVA DUPLICATION IN DOGS     

Giovanna Bertolini  Alessia Diana  Mario Cipone  Michele Drigo  Marco Caldin 《Veterinary radiology & ultrasound》2014,55(5):521-530

Caudal vena cava duplication has been rarely reported in small animals. The purpose of this retrospective study was to describe characteristics of duplicated caudal vena cava in a large group of dogs. Computed tomography (CT) and ultrasound databases from two hospitals were searched for canine reports having the diagnosis “double caudal vena cava.” One observer reviewed CT images for 71 dogs and two observers reviewed ultrasound images for 21 dogs. In all CT cases, the duplication comprised two vessels that were bilaterally symmetrical and approximately the same calibre (similar to Type I complete duplication in humans). In all ultrasound cases, the duplicated caudal vena cava appeared as a distinct vessel running on the left side of the abdominal segment of the descending aorta and extending from the left common iliac vein to the left renal vein. The prevalence of caudal vena cava duplication was 0.46% for canine ultrasound studies and 2.08% for canine CT studies performed at these hospitals. Median body weight for affected dogs was significantly lower than that of unaffected dogs (P < 0.0001). Breeds with increased risk for duplicated caudal vena cava were Yorkshire Terrier (odds ratio [OR] = 6.41), Poodle (OR = 7.46), West Highland White Terrier (OR = 6.33), and Maltese (OR = 3.87). Presence of a duplicated caudal vena cava was significantly associated with presence of extrahepatic portosystemic shunt(s) (P < 0.004). While uncommon in dogs, caudal vena cava duplication should be differentiated from other vascular anomalies when planning surgeries and for avoiding misdiagnoses.  相似文献