Gastric inflammatory pseudotumour secondary to <Emphasis Type="Italic">Actinomyces hordeovulneris</Emphasis> infection in a cat     

Marco Pietra  Renato Giulio Zanoni  Angelo Peli  Barbara Brunetti  Nikolina Linta  Ombretta Capitani  Giuseppe Spinella 《Irish veterinary journal》2015,69(1):12

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Comparison of pulmonary artery and transpulmonary thermodilution cardiac output measurements in unsedated newborn calves          下载免费PDF全文

Annette PN Kutter  Rahel S Jud Schefer  Barbara Bircher  Uli Bleul  Regula Bettschart‐Wolfensberger 《Veterinary anaesthesia and analgesia》2015,42(6):614-622

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Assessment of coagulation utilizing thromboelastometry in dogs undergoing orthopedic surgery          下载免费PDF全文

Barbara Bruno DVM  Cristiana Maurella DVM  Sara Falco DVM  Alberto Tarducci DVM  Renato Zanatta DVM  PhD  Paola Gianella DVM  PhD  DACVIM  Antonio D'Angelo DVM  PhD  DECVN  DECBHM  Lisa Piras DVM  PhD  Andrea Di Bella DVM  MRCVS  Antonio Borrelli DVM  PhD  MS 《Journal of Veterinary Emergency and Critical Care》2015,25(3):358-363

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Brazilian savanna re-establishment in a monoculture forest:diversity and environmental relations of native regenerating understory in Pinus caribaea Morelet. stands     

Gastao Viegas de Pinho Ju nior  Andre R Terra Nascimento  Barbara Tahara Valverde  Lucas H Clemente 《林业研究》2015,(3)

In this paper we analyze and compare natural regeneration in shrub-tree community in three areas below Pinus caribaea stands and a natural area (cerrado sensu stricto). We also analyze the influence of...  相似文献   

Real-time TaqMan polymerase chain reaction detection and quantification of cow DNA in pure water buffalo mozzarella cheese: method validation and its application on commercial samples     

Lopparelli RM  Cardazzo B  Balzan S  Giaccone V  Novelli E 《Journal of agricultural and food chemistry》2007,55(9):3429-3434

Mozzarella cheese obtained from buffalo (Bubalus bubalis) milk is a typical Italian product certificated by means of the European Protected Designation of Origin (PDO). Mozzarella cheese can also be obtained from bovine milk or bovine/buffalo milk mixtures, but in this case, it cannot be sold as PDO product, and its label must report the actual ingredients. However, bovine milk in PDO products was frequently detected in the past, suggesting fraudulent addition or accidental contamination. Several methods based on end-point polymerase chain reaction (PCR) have been profitably applied in a large number of tests to detect the presence of undeclared ingredients, also in dairy products. In the present study we report a real-time PCR method able to quantify bovine milk addition to pure buffalo cheese products. We validated a normalized procedure based on two targets: bovine mitochondrial cytochrome b (cyt b) to detect and quantify the bovine DNA and nuclear growth hormone (GH) gene used as a universal reference marker. With the use of this real-time PCR assay, 64 commercial mozzarella di bufala cheese samples purchased at local supermarkets, dairy shops, or directly from cheese manufacturers were analyzed. The results obtained demonstrate that most of the commercial samples were contaminated with bovine milk. Therefore, this assay could be conveniently employed to carry out routine and accurate controls aimed not only to discourage any fraudulent behavior but also to reduce risks for consumer health.  相似文献   

Evaluation of harmful algal bloom outreach activities          下载免费PDF全文

Fleming LE  Jerez E  Stephan WB  Cassedy A  Bean JA  Reich A  Kirkpatrick B  Backer L  Nierenberg K  Watkins S  Hollenbeck J  Weisman R 《Marine drugs》2007,5(4):208-219

With an apparent increase of harmful algal blooms (HABs) worldwide, healthcare providers, public health personnel and coastal managers are struggling to provide scientifically-based appropriately-targeted HAB outreach and education. Since 1998, the Florida Poison Information Center-Miami, with its 24 hour/365 day/year free Aquatic Toxins Hotline (1–888–232–8635) available in several languages, has received over 25,000 HAB-related calls. As part of HAB surveillance, all possible cases of HAB-related illness among callers are reported to the Florida Health Department. This pilot study evaluated an automated call processing menu system that allows callers to access bilingual HAB information, and to speak directly with a trained Poison Information Specialist. The majority (68%) of callers reported satisfaction with the information, and many provided specific suggestions for improvement. This pilot study, the first known evaluation of use and satisfaction with HAB educational outreach materials, demonstrated that the automated system provided useful HAB-related information for the majority of callers, and decreased the routine informational call workload for the Poison Information Specialists, allowing them to focus on callers needing immediate assistance and their healthcare providers. These results will lead to improvement of this valuable HAB outreach, education and surveillance tool. Formal evaluation is recommended for future HAB outreach and educational materials.  相似文献   

Characterization of aerosols containing microcystin          下载免费PDF全文

Cheng YS  Zhou Y  Irvin CM  Kirkpatrick B  Backer LC 《Marine drugs》2007,5(4):136-150

Toxic blooms of cyanobacteria are ubiquitous in both freshwater and brackish water sources throughout the world. One class of cyanobacterial toxins, called microcystins, is cyclic peptides. In addition to ingestion and dermal, inhalation is a likely route of human exposure. A significant increase in reporting of minor symptoms, particularly respiratory symptoms was associated with exposure to higher levels of cyanobacteria during recreational activities. Algae cells, bacteria, and waterborne toxins can be aerosolized by a bubble-bursting process with a wind-driven white-capped wave mechanism. The purposes of this study were to: evaluate sampling and analysis techniques for microcystin aerosol, produce aerosol droplets containing microcystin in the laboratory, and deploy the sampling instruments in field studies. A high-volume impactor and an IOM filter sampler were tried first in the laboratory to collect droplets containing microcystins. Samples were extracted and analyzed for microcystin using an ELISA method. The laboratory study showed that cyanotoxins in water could be transferred to air via a bubble-bursting process. The droplets containing microcystins showed a bimodal size distribution with the mass median aerodynamic diameter (MMAD) of 1.4 and 27.8 mum. The sampling and analysis methods were successfully used in a pilot field study to measure microcystin aerosol in situ.  相似文献   

Isolation and determination of alpha-dicarbonyl compounds by RP-HPLC-DAD in green and roasted coffee     

Daglia M  Papetti A  Aceti C  Sordelli B  Spini V  Gazzani G 《Journal of agricultural and food chemistry》2007,55(22):8877-8882

Glyoxal, methylglyoxal, and diacetyl formed as Maillard reaction products in heat-treated food were determined in coffee extracts (coffee brews) obtained from green beans and beans with different degrees of roast. The compounds have been reported to be mutagenic in vitro and genotoxic in experimental animals in a number of papers. More recently, alpha-dicarbonyl compounds have been implicated in the glycation process. Our data show that small amounts of glyoxal and methylglyoxal occur naturally in green coffee beans. Their concentrations increase in the early phases of the roasting process and then decline. Conversely, diacetyl is not found in green beans and forms later in the roasting process. Therefore, light and medium roasted coffees had the highest glyoxal and methylglyoxal content, whereas dark roasted coffee contained smaller amounts of glyoxal, methylglyoxal, and diacetyl. For the determination of coffee alpha-dicarbonyl compounds, a reversed-phase high performance liquid chromatography with a diode array detector (RP-HPLC-DAD) method was devised that involved the elimination of interfering compounds, such as chlorogenic acids, by solid phase extraction (SPE) and their derivatization with 1,2-diaminobenzene to give quinoxaline derivatives. Checks of SPE and derivatization conditions to verify recovery and yield, respectively, resulted in rates of 100%. The results of the validation procedure showed that the proposed method is selective, precise, accurate, and sensitive.  相似文献   

Apple peel as a contributor to whole fruit quantity of potentially healthful bioactive compounds. Cultivar and year implication     

Łata B  Tomala K 《Journal of agricultural and food chemistry》2007,55(26):10795-10802

On the basis of the fresh weight of apple fruit and its peel and the concentration of bioactive compounds, the total quantity of L-cysteine, glutathione, ascorbate, flavonols, and anthocyanins as well as phenolics was evaluated in a wide range of cultivars and two growing seasons. Apple peel as a contributor to the entire apple quantity of the examined components considerably differed in relation to the investigated compounds and was also highly cultivar dependent. A great amount of flavonols was found in apple peel (approximately 40%), followed by ascorbate (approximately 30%) and total phenolics (approximately 20%), while the lowest contribution was assessed for thiols (approximately 11% and 14% for L-cysteine and total quantity of glutathione, respectively), based on average values for both years. Seasonal variations in the quantity of antioxidants was more pronounced in apple peel, whereas the contribution of apple peel to the whole fruit was predominantly affected by the genotype. A very high positive correlation existed between apple peel and the whole fruit quantity of antioxidants.  相似文献   

The conservation of the endangered Mediterranean common dolphin (Delphinus delphis): Current knowledge and research priorities     

Adriana Vella  Sinéad Murphy  Joan Giménez  Renaud de Stephanis  Barbara Mussi  Joseph G. Vella  Kamel Larbi Doukara  Daniela Silvia Pace 《水产资源保护:海洋与淡水生态系统》2021,31(Z1):110-136

1. Preservation of endangered species is now recognized as a relevant component of regional and global conservation actions achieved through the maintenance of the remaining populations in a favourable conservation status. The Mediterranean common dolphin (Delphinus delphis) is listed as an Endangered subpopulation in the International Union for Conservation of Nature Red List, with declining numbers in some of the areas where it has been studied for numerous years. Prey depletion, fisheries bycatch, pollution, health risks, and climate change are among the recognized threats that the species is facing in the region.
2. The existing national, regional, and international legislation pertinent to Mediterranean countries should aid greater science–policy–management integration and support innovative research and monitoring towards effective conservation. The challenges inherent in studying the pelagic D. delphis should not preclude the use of long-term, year-round, multiplatform surveys, side by side with the latest molecular tools, which aid our understanding of its populations' genetic diversity, resistance to diseases, and resilience in a semi-enclosed fast-changing sea.
3. Addressing the multiplicity of problems confronting the species requires collaborative effort at all levels to share and merge resources, data, and expertise more efficiently. In this review, we provide an overview of the current knowledge on the Mediterranean common dolphin, the main threats, and knowledge gaps that are priorities for addressing its conservation, while providing clear recommendations for effectively safeguarding the species and its habitat at both local and regional levels.

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