Phytosterol-enriched products on the Irish market: examination of intake and consumption patterns     

Hearty A  Duffy E  Joyce J  O'Connor C  Gibney MJ 《Public health nutrition》2009,12(1):51-58

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Medicinal potential from in vivo and acclimatized plants of Cleome rosea     

Simões C  De Mattos JC  Sabino KC  Caldeira-de-Araújo A  Coelho MG  Albarello N  Figueiredo SF 《Fitoterapia》2006,77(2):94-99

Methanolic extracts obtained from different organs of Cleome rosea, collected from its natural habitat and from in vitro-propagated plants, were submitted to in vitro biological assays. Inhibition of nitric oxide (NO) production by J774 macrophages and antioxidant effects by protecting the plasmid DNA from the SnCl(2)-induced damage were evaluated. Extracts from the stem of both origins and leaf of natural plants inhibited NO production. The plasmid DNA strand breaks induced by SnCl(2) were reduced by extracts from either leaf or stem of both sources. On the other hand, root extracts did not show any kind of effects on plasmid DNA, and presented significant toxic effects to J774 cells. The results showed that C. rosea presents medicinal potential and that the acclimatization process reduces the plant toxicity both to plasmid DNA and to J774 cells, suggesting the use of biotechnology tools to obtain elite plants as source of botanical material for pharmacological and phytochemical studies.  相似文献   

Development of NIRS equations for food grain quality traits through exploitation of a core collection of cultivated sorghum     

de Alencar Figueiredo LF  Davrieux F  Fliedel G  Rami JF  Chantereau J  Deu M  Courtois B  Mestres C 《Journal of agricultural and food chemistry》2006,54(22):8501-8509

A sorghum core collection representing a wide range of genetic diversity and used in the framework of a sorghum breeding and genetics program was evaluated by near-infrared reflectance spectroscopy (NIRS) to predict food grain quality traits: amylose content (AM), protein content (PR), lipid content (LI), endosperm texture (ET), and hardness (HD). A total of 278 sorghum samples were scanned as whole and ground grain to develop calibration equations. Laboratory analyses were performed on NIRS sample subsets that preserved the core collection racial distribution. Principal component analysis performed on NIRS spectra evidenced a level of structure following known sorghum races, which underlined the importance of using a wide range of genetic diversity. Performances of calibration equations were evaluated by the coefficient of determination, bias, standard error of laboratory (SEL), and ratio of performance deviation (RPD). Ground grain spectra gave better calibration equations than whole grain. PR equation (RPD of 5.7) can be used for quality control. ET, LI, and HD equations (RPD of 2.9, 2.6, and 2.6, respectively) can be used for screening steps. Even with a small SEL in whole sample analysis, a RPD of 1.8 for AM confirmed that this variable is not easy to predict with NIRS.  相似文献   

The veterinary quarterly: Changing to a review journal on animal diseases     

W. Edel Chairman Editorial Board DVM.PhD 《The Veterinary quarterly》2013,33(4)

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Mutational analysis of type III effector genes from <Emphasis Type="Italic">Xanthomonas citri</Emphasis> subsp. <Emphasis Type="Italic">citri</Emphasis>     

Jose Francisco L. Figueiredo  Gerald V. Minsavage  James H. Graham  Frank F. White  Jeffrey B. Jones 《European journal of plant pathology / European Foundation for Plant Pathology》2011,130(3):339-347

Xanthomonas citri subsp. citri, the causal agent of citrus canker, relies extensively on a type III secretion system for infection by delivering type III effectors into host cells. In the genus Xanthomonas, two major regulators, HrpG and HrpX, are involved in the expression of genes encoding the type III secretion system. Twenty-three candidate type III effectors were identified as targets for analysis. The involvement in pathogenicity of 20 candidate effector genes in X. citri strain 306 (Xcc-306) was investigated using site-directed mutagenesis. Pathogenicity assays in grapefruit of 19 genes using site-directed mutagenesis revealed that none of the mutants demonstrated to have reduced ability to cause canker disease. A mutation in the TAL effector pthA4 ^– resulted in loss of hypertrophy although no changes were observed in bacterial growth in leaves. Mutations in hrpG, hrpX, or hrpA genes displayed a complete loss of pathogenicity. Moreover, all mutants maintained the ability to trigger a hypersensitive response (HR) in non-host tomato. In contrast to previous studies, hrpG ^– , hrpX ^– and hrpA ^– mutants also retained the ability to elicit an HR in tomato, indicating the presence of an Hrp independent elicitor in Xcc-306.  相似文献   

Pharmacology and therapeutics in donkeys     

D. A. Grosenbaugh  C. R. Reinemeyer  M. D. Figueiredo 《Equine Veterinary Education》2011,23(10):523-530

Therapeutics are often administered to donkeys based on dosage and intervals recommended for horses because very few drugs have donkey‐specific label indications. Yet differences between donkeys and horses in drug distribution, metabolism and elimination have been noted for most therapeutic agents studied. These differences can be partially explained by the donkey's unique physiology. Since their ancestors evolved in a desert environment, the modern donkey exhibits qualities that allow them to tolerate dehydration better than the horse and recover more quickly from its effects. Fluid balance and body water compartment partitioning differ from the horse and may have implications regarding drug distribution. Since donkeys are preferential browsers, differences in diet may have influenced evolutionary differences in metabolic disposition of drugs. It is important to acknowledge these differences when designing dose regimes for donkeys based on horse protocols in order to avoid either lack of efficacy or toxicity.  相似文献   

Survival and early growth of mixed forest stands installed in a Mediterranean Region: Effects of site preparation intensity     

Felícia Fonseca  Tomás de Figueiredo  Afonso Martins 《Forest Ecology and Management》2011,262(10):1905-1912

In Mediterranean environments, availability of water and nutrients are the main factors limiting the success of afforestation. As part of a wider project, an experiment was established in Northeast Portugal, aiming at testing the effect of several site preparation techniques on plant survival and growth (height and diameter) in a newly installed mixed forest stand. Results presented regard plant response during 42 months after plantation. The experimental protocol consisted in seven treatments described by mechanical operations that rank soil disturbance intensity from none to high, set in plots of 375 m², randomly distributed in three blocks, in different topographic positions (gentle slope plateau, moderate slope shoulder, and steep mid-slope). Pseudotsuga menziesii (PM) and Castanea sativa (CS) forest species were planted in a 4 m × 2 m scheme and in alternate rows with 12 plants on each row per plot, summing up 72 plant per specie and treatment at start of the experiment. The results show that: (i) the highest mortality was observed immediately after the plantation and before the dry season, on the lowest intensity treatments; (ii) after the dry season, the highest mortality was also observed in treatments with the lowest intensity of soil disturbance, while the lowest values were found on the intermediate intensity treatments; (iii) during the experimental period, the effect of treatments on plant growth (height and diameter) was statistically significant; however, experimental results do not lead yet to a clear quantitative relationship between soil disturbance intensity due to site preparation and plant response under the conditions tested.  相似文献   

Fresh and stored copra meal in meat quail diets     

Farias  Nadja Naiara Pereira  Freitas  Ednardo Rodrigues  do Nascimento  Germano Augusto Jerônimo  Xavier  Regina Patrícia Souza  de Melo Braz  Nádia  Dantas  Francisco Diego Teixeira  Figueiredo  Carlos Weiber Silva  Gomes  Valter Luis Maia  Watanabe  Pedro Henrique 《Tropical animal health and production》2019,51(1):179-185

Tropical Animal Health and Production - The aim of the current study was to evaluate the addition of fresh and stored copra meal to the diet of meat quails. Two hundred eighty-seven-day-old male...  相似文献   

Cervical, mandibular, and parotid lymph nodes of dogs naturally infected with Leishmania infantum: a histopathologic and immunohistochemistry study and its correlation with facial skin lesions     

Costa MM  Lima WG  Figueiredo MM  Michalick MS  Tafuri WL  Tafuri WL 《Veterinary pathology》2008,45(5):613-616

The parasite load in cervical, mandibular, and parotid lymph nodes and in the skin of the nose and the pinna from dogs infected with Leishmania infantum were investigated by histologic and immunohistochemical studies. Twenty-two infected dogs with and without signs of infection were examined to demonstrate correlation of signs with parasite load and the correlation of facial skin lesions with parasites in regional lymph nodes. Chronic inflammation of the skin was demonstrated in infected dogs that had no gross skin lesions, confirming that normal-appearing skin can harbor the parasite, likely playing a role in transmission. Dogs with facial skin lesions showed a higher parasite load in parotid lymph nodes than dogs without lesions of the facial skin, based on Leishman-Donovan unit analysis. Based on immunohistochemical analysis, parasite load in parotid and cervical nodes was correlated with that of skin of the nose and pinna, as was the parasite load in mandibular lymph nodes and skin of the external nose. We demonstrated a logical involvement of the lymphatic vessels and their specific anatomic draining sites.  相似文献   

Antiviral Sulfoquinovosyldiacylglycerols (SQDGs) from the Brazilian Brown Seaweed Sargassum vulgare     

Erwan Plouguerné  Lauro M. de Souza  Guilherme L. Sassaki  Jéssica Figueiredo Cavalcanti  Maria Teresa Villela Romanos  Bernardo A. P. da Gama  Renato Crespo Pereira  Eliana Barreto-Bergter 《Marine drugs》2013,11(11):4628-4640

Total lipids from the Brazilian brown seaweed Sargassum vulgare were extracted with chloroform/methanol 2:1 and 1:2 (v/v) at room temperature. After performing Folch partition of the crude lipid extract, the lipids recovered from the Folch lower layer were fractionated on a silica gel column eluted with chloroform, acetone and methanol. The fraction eluted with methanol, presented a strong orcinol-positive band characteristic of the presence of sulfatides when examined by TLC. This fraction was then purified by two successive silica gel column chromatography giving rise to fractions F4I86 and F4II90 that exhibited strong activity against herpes simplex virus type 1 and 2. The chemical structures present in both fractions were elucidated by ESI-MS and ¹H/¹³C NMR analysis HSQC fingerprints based on their tandem–MS behavior as sulfoquinovosildiacylglycerols (SQDGs). The main SQDG present in both fractions and responsible for the anti-herpes activity observed was identified as 1,2-di-O-palmitoyl-3-O-(6-sulfo-α-d-quinovopyranosyl)-glycerol.  相似文献