Effects of Acepromazine on Equine Polymorphonuclear Neutrophil Activation: A Chemiluminescence Study     

D. SERTEYN  H. BENBAREK  G. DEBY-DUPONT  S. GRULKE  I. CAUDRON  C. DE BY  M. LAMY   《Veterinary journal (London, England : 1997)》1999,157(3):332-335

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Covariation in climate, zooplankton biomass and mackerel recruitment in the southern Gulf of St Lawrence   总被引：1，自引：0，他引：1  

RUNGE  CASTONGUAY  Y. DE LAFONTAINE  RINGUETTE  & BEAULIEU 《Fisheries Oceanography》1999,8(2):139-149

Between 1982 and 1991, an annual survey of stage I egg production of Atlantic mackerel ( Scomber scombrus ) was conducted in June/early July in the southern Gulf of St Lawrence. We investigated the relationship between interannual variability in biomass of zooplankton, determined from the archived survey plankton samples, and mackerel recruitment, estimated from the proportion of three-year-olds in the catch of the commercial fishery. Zooplankton biomass varied by a factor of 2.5, primarily owing to fluctuations in the >1000 μm size fraction. The index of mackerel recruitment fluctuated by a factor of ≈20 and was positively related (linear regression: P  < 0.05; n  = 10) to variations in the zooplankton biomass. Both mackerel recruitment and zooplankton biomass were negatively related (linear regression: P  < 0.05) to RIVSUM, a measure of freshwater discharge from the St Lawrence River system and an index of variability in the region's climate. Three hypotheses are put forward to explain these observations: (1) there is a strong link between interannual variation in abundance of copepod females, which produce prey for mackerel larvae, and larval survival; the exceptional recruitment and subsequent year class resulted from an exceptional production of Calanus finmarchicus nauplii; (2) years of high zooplankton biomass provide better feeding conditions and consequently higher survival of mackerel juveniles; and (3) mackerel recruitment and zooplankton biomass are independently under the control of an underlying physical process, without strong trophic linkage. The first hypothesis is supported by a study of copepod species composition and female abundance conducted for four of the survey years. At the present time, none of these hypotheses can be ruled out.  相似文献   

Habitat suitability modelling for sardine juveniles (Sardina pilchardus) in the Mediterranean Sea     

MARIANNA GIANNOULAKI  MARIA M. PYROUNAKI  BERNARD LIORZOU  IOLE LEONORI  VASILIS D. VALAVANIS  KONSTANTINOS TSAGARAKIS  JEAN L. BIGOT  DAVID ROOS  ANDREA DE FELICE  FABIO CAMPANELLA  STYLIANOS SOMARAKIS  ENRICO ARNERI  ATHANASSIOS MACHIAS 《Fisheries Oceanography》2011,20(5):367-382

Identification of potential juvenile grounds of short‐lived species such as European sardine (Sardina pilchardus) in relation to the environment is a crucial issue for effective management. In the current work, habitat suitability modelling was applied to acoustic data derived from both the western and eastern part of the Mediterranean Sea. Early summer acoustic data of sardine juveniles were modelled using generalized additive models along with satellite environmental and bathymetry data. Selected models were used to construct maps that exhibit the probability of presence in the study areas, as well as throughout the entire Mediterranean basin, as a measure of habitat adequacy. Areas with high probability of supporting sardine juvenile presence persistently within the study period were identified throughout the Mediterranean Sea. Furthermore, within the study period, a positive relationship was found between suitable habitat extent and the changes in abundance of sardine juveniles in each study area.  相似文献   

Short-term food deprivation does not improve the efficacy of a fish oil finishing strategy in Murray cod     

G. PALMERI  G.M. TURCHINI  & S.S. DE SILVA 《Aquaculture Nutrition》2009,15(6):657-666

Two groups of fish ( Maccullochella peelii peelii ) were fed for a 90-day conditioning period on a canola oil diet (CO) or a fish oil diet (FO). Canola oil diet fed fish were then shifted to the FO diet for a 90-day finishing period. A variable period of starvation (0, 5, 10 and 15 days) was introduced to reduce the initial lipid level of CO fed fish at the beginning of the finishing period and therefore accelerate the rate of recovery of FO-like fatty acids. During starvation, fish did not show significant reduction in total lipid content, either in the fillet or whole body. At the end of the conditioning period, fatty acid composition of the diet was mirrored in fish tissues. These differences came close to levelling out following re-feeding, with the exception of n  - 6 polyunsaturated fatty acids (PUFA). However, no effects of the starvation periods on the final fatty acid make-up of fish were recorded. The results of this trial show that Murray cod, when subjected to a starvation period of up to 15 days, does not lose an appreciable quantity of lipid and, therefore, the tested starvation approach to reduce the initial level of lipid has to be considered unsuccessful.  相似文献   

Monthly assessments of proteins, fatty acids and amino acids in Artemia franciscana cultivated in 'Las Salinas de Hidalgo', state of San Luis Potosí, Mexico     

T. CASTRO  H. SANDOVAL  A. CASTRO  J. CASTRO  G. CASTRO  R. DE LARA  & L.H. HERNANDEZ 《Aquaculture Nutrition》2009,15(2):123-128

This study presents the biochemical contents of Artemia biomass produced in a pond in 'Las Salinas de Hidalgo', State of San Luis Potosí, Mexico. The culture was realized over a period of 1 year in an 80 × 50 × 0.65 m (200 m³) pond. The feed consisted of a mixture of four micro algae. Samples of 1 kg of Artemia biomass (wet basis) were taken monthly and total protein, total lipid, amino acids and fatty acids were assessed. The contents of total protein and lipids showed a similar tendency from July to December (maintained values of about 300 mg g⁻¹ for protein and 90 mg g⁻¹ for lipids). With the exception of methionine and arginine, other seven indispensable amino acids were detected in the monthly samples, having similar values during the period from July to December. The most common fatty acids determined were the C16, C18, C18:1 and C18:3 n 6. Both, C20:4 n 6 and C20:5 n 3, were observed occasionally, but in high quantities. The use of the four micro algae as food for the Artemia cultured extensively, improved the biochemical composition and allows using this crustacean as feed for several species of aquatic organisms used for ornamental or human consumption.  相似文献   

播种方式对燕麦和饲用豌豆饲草产量及品质的影响     

郭常英  王伟  德科加  蒲小剑  魏晓丽  段娜宁  彭丹  徐成体 《草地学报》2022,30(7):1882-1890

为研究青海省适宜的禾豆混播方式，本研究以行距为30 cm,25 cm,20 cm和15 cm混播、间作、单播燕麦(Avena sativa)和饲用豌豆(Pisum sativum)两种饲草，分析研究不同混播方式下饲草产量及营养品质，以期为青藏高原高寒地区燕麦与饲用豌豆混播草地的建植提供参考。结果表明：间混作对饲草相对生长速率及饲草总产量均具有显著影响，其中混播处理的草产量高于间作处理。不同播种行距对饲草产量具有显著影响，饲草产量从高到低依次排序为：30 cm>25 cm>15 cm>20 cm。间混作对饲草营养成分具有显著影响，其中混播处理的粗蛋白含量高于间作处理，中性洗涤纤维含量低于间作处理，说明同行混播更有利于提高饲草品质；行距为30 cm与25 cm时，其各营养指标相对优于其他两个行距。通过合理的群体空间结构配置可以提高草产量，其中混播间距30 cm处理综合表现最优，其次为间作行距30 cm播种。  相似文献   

基于单细胞转录组测序技术对鄂尔多斯细毛羊次级毛囊形态发生诱导期的研究     

赫雪  谷英  斯登丹巴  德德玛  窦骁宁  吴怡  雷志惠  潘和平  岳耀敬 《中国畜牧兽医》2022,49(11):4296-4306

【目的】 试验旨在解析鄂尔多斯细毛羊胚胎期次级毛囊诱导期形态发生过程中主要细胞类型的分子特征和分化过程。【方法】 对采集的3只鄂尔多斯细毛羊（胎龄87 d）体侧部（肩胛骨后缘处）的皮肤样本进行HE染色，鉴定毛囊的发育时期；部分皮肤样本经过混样后进行单细胞转录组测序（scRNA-Seq），应用t-分布随机近邻嵌入（tSNE）分析细胞簇，分别使用胶原Ⅰ型α1链（Col1a1）和角蛋白15（Krt15）鉴定真皮谱系细胞和表皮谱系细胞，并使用皮肤组织不同细胞的标记基因进行细胞类型分析；对测序数据进行拟时序分析，探究其分化过程中差异基因的表达；通过GO功能富集分析进一步验证基因的功能。【结果】 HE染色结果发现，鄂尔多斯细毛羊在胎龄87 d处于次级毛囊诱导期。通过scRNA-Seq在胎龄87 d的细毛羊体侧部皮肤细胞样品中获得10 603个细胞和18 704个基因的scRNA-Seq数据可用于下游分析。tSNE分析发现，皮肤组织中共有15个细胞簇；Col1a1和Krt15标记基因鉴定表明，真皮细胞和表皮细胞具有高度异质性。拟时序分析构建毛囊形态发生过程中真皮/表皮细胞谱系细胞的分化轨迹和基因动态表达图谱表明，在真皮谱系细胞由成纤维细胞（Fb）向成熟真皮聚凝物（DC）的分化过程中，多个不同阶段的标记基因FST重组蛋白（Fst）、抑制素亚基βα（Inhba）和转录阻遏物GATA结合1（Trps1）均在拟时序轨道上特异性表达，并富集了Wnt、Noggin和骨形态发生蛋白（BMP）等与毛囊形态发生相关的信号通路；在表皮谱系细胞分化过程中，基质和毛囊间表皮（IFE）的标记基因角蛋白10（Krt10）、同源基因C13（HOXC13）和音猬因子信号（SHH）在表皮谱系细胞拟时序轨道的2个分支上均特异性表达，且富集在细胞增殖和细胞黏附等相关通路。【结论】 在细毛羊次级毛囊诱导期，真皮谱系细胞由成纤维细胞分化至真皮聚凝物，表皮谱系细胞处于基质和毛囊间表皮细胞增殖分化阶段，结果可加深人们对细毛羊次级毛囊形态发生过程的了解，为鄂尔多斯细毛羊育种研究提供有力的理论和技术支持。  相似文献   

三江源区小黑麦与豆科饲草混播最佳组合及比例研究          下载免费PDF全文

冯廷旭  德科加  向雪梅  魏希杰  徐成体  王伟  钱诗祎  林伟山 《西北农业学报》2023,(2):232-241

研究不同饲草禾豆混播组合及比例对三江源玉树地区人工草地的建植具有重要的科学意义。为筛选出最佳混播组合及比例，以小黑麦(Triticale)、箭筈豌豆(Vicia sativa)、饲用豌豆(Pisum sativa)为试验对象，研究不同混播组合及比例对饲草产量及品质的影响。结果表明：小黑麦+箭筈豌豆、小黑麦+饲用豌豆两种混播处理下，饲草干草产量比单播小黑麦分别提高19.50%、5.49%,比单播豆科饲草产量分别提高55.17%、111.97%。随着各混播处理中豆科饲草播量增加，饲草粗蛋白含量逐渐升高，而中性洗涤纤维、酸性洗涤纤维含量均逐渐降低。土壤全磷、全氮、有机碳养分含量均呈现增加趋势。运用灰色关联度对饲草进行综合评价，小黑麦+箭筈豌豆(7∶3)较其他处理的混播处理具有更好的产草量以及更优的饲草品质，小黑麦+饲用豌豆(5∶5)与其余混播处理相比较在产草量及品质方面有更大潜力。  相似文献   

高寒地区燕麦与豌豆不同混播组合和比例对饲草产量及品质的影响     

冯廷旭  德科加  向雪梅  魏希杰  徐成体  王伟  林伟山  钱诗祎 《草地学报》2022,30(2):487-494

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高寒地区燕麦与豌豆不同混播组合和比例对饲草产量及品质的影响     

冯廷旭  德科加  向雪梅  魏希杰  徐成体  王伟  林伟山  钱诗祎 《草地学报》2022,30(2):487-494

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