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411.
Acaricide resistance is a major problem that hinders the control of the tropical cattle tick, Rhipicephalus (Boophilus) microplus (Canestrini), in many parts of the world where cattle production continues to suffer severe economic losses to tick infestation. Deltamethrin and amitraz have been used alone to control R. microplus in New Caledonia for the past decade, and tick populations have developed resistance to both acaricides. A study was conducted to evaluate the effectiveness of deltamethrin and amitraz mixtures, through in vitro laboratory bioassays and in vivo on-animal efficacy trials, for the control of resistant R. microplus on cattle at two dairy farms in New Caledonia. Results of laboratory bioassays using modified larval packet tests (LPT) revealed up to 16.59-fold resistance to deltamethrin, and up to 5.86-fold resistance to amitraz. Significant synergism was observed when amitraz was used as a synergist in deltamethrin bioassays. Amitraz significantly increased deltamethrin toxicity to tick larvae, while deltamethrin was much less effective on amitraz toxicity. Synergism of amitraz by deltamethrin only occurred when the deltamethrin concentration was relatively high. Results of on animal efficacy trials of deltamethrin and amitraz alone and mixtures of both at different concentrations revealed a similar pattern of synergism. Adding amitraz to a deltamethrin formulation led to dramatic increases of percent reduction of both immature and adult ticks. In contrast, adding deltamethrin to an amitraz formulation did not increase control efficacy. Results from this study may lead to the adoption of an acaricide mixture strategy for the control of pyrethroid-resistant R. microplus in New Caledonia and elsewhere.  相似文献   
412.
Radiographs of vertebral segments from 200 cats were retrospectively assessed to determine the incidence of congenital axial skeletal abnormalities. The axial skeleton was divided into cervical, thoracic, lumbar, sacral, and caudal segments and the presence and types of congenital abnormalities present were recorded for each segment. A total of 598 axial skeletal segments were examined. Congenital abnormalities were found in 46 cats and involved 54 spinal segments. Block vertebrae were identified in three cats. No hemivertebrae or spina bifida were noted. Transitional abnormalities were most common, and were identified in 51 spinal segments. Abnormalities at the sacrocaudal junction were the most common of these; 19 examples were found. The second most common transitional abnormality was thoracicization of L1, with 18 such cats identified. Extra ribs on L1 were often smaller and fragmented and it may be possible for an inexperienced clinician to confuse these with rib fractures. The last rib(s) are also often used for anatomic localization of surgical procedures; the presence of a transitional lumbar vertebra with one or two extra ribs introduces the possibility of error or confusion about choice of surgical site.  相似文献   
413.
Mineralization of paraprostatic cysts is reported to be uncommon. This retrospective study was performed to review the imaging findings of eight histologically confirmed canine paraprostatic cysts. Radiographic patterns of mineralization seen are described. Four of the eight dogs had mineralized cysts. Mineralization seen on radiography was not consistently seen on ultrasonography, probably due to the lack of acoustic shadowing artefact from the small depth of mineralized tissue. It is thus concluded that mineralization of paraprostatic cysts is more common than implied in the literature, and that radiography is superior to ultrasonography in identifying its presence.  相似文献   
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A 2-year-old, captive-born, clinically healthy male, rhesus macaque, was euthanatized as part of an experimental study. At necropsy, diffuse pale streaking of the trunk, lumbar, and limb muscles were noted macroscopically. On histology, numerous elongated cysts that contained crescent-shaped basophilic spores were found in the fibers of skeletal muscles. Scattered affected myofibers were degenerate and accompanied by eosinophilic-to-granulomatous inflammation. Sarcocysts had prominent villus-like projections with the morphology of a type 11 sarcocyst wall similar to Sarcocystis neurona but possessing many more villus microtubules than is reported for S. neurona. In addition, bradyzoites were very long, up to approximately 12 microm in length. The protozoa were consistent with a Sarcocystis sp., based on histology and ultrastructure, however, a definitive identification of the species was not possible. Nonspecific immunohistochemical crossreaction with Sarcocystis cruzi antisera was observed. The 18S ribosomal deoxyribonucleic acid sequence showed 91% similarity to Sarcocystis hominis, 90% similarity to Sarcocystis buffalonis, and 89% similarity to Sarcocystis hirsuta. Interestingly, the ITS1 sequence showed very little homology to any sequence in GenBank, suggesting that this is possibly a unique Sarcocystis sp. Sarcocystosis is often considered an incidental finding, particularly in wild-caught animals, with little clinical significance. However, as demonstrated in this report and others, disseminated sarcocystosis can occur in captive-born rhesus macaques with or without clinical signs. In some cases interference with research results can occur; including death in fulminant cases.  相似文献   
419.
The Khapra beetle, Trogoderma granarium Everts, is a global pest species of stored grain products. Accurate identification of Trogoderma specimens trapped within stores of grain is critical to surveillance and exclusion efforts for the Khapra beetle. To enhance diagnostic capacity, we report PCR-based diagnostic assays for Khapra beetle identification. Three methods such as conventional PCR, real-time PCR, and DNA sequencing are reported for the diagnosis of Trogoderma specimens captured within the U.S. All three methods discern the Khapra beetle based on variation in fragments of 16S mitochondrial DNA. To examine PCR assay stringency, ten native or introduced Trogoderma species and two Megatoma species were also examined. These DNA-based assays provide reliable identification of T. granarium regardless of condition, life stage, or taxonomic expertise of the investigator.  相似文献   
420.
Barr S 《Science (New York, N.Y.)》1982,216(4550):1111-1112
A fortuitous and dramatic photograph of the Soufriere eruption column of 17 April 1979 displays a series of highly structured skirt clouds. The gentle distortion of thin, quasi-horizontal layers of moist air has been documented in meteorological situations. It is proposed that at St. Vincent subhorizontal layers of moist air were intensely deformed by the rapidly rising eruption column and were carried to higher altitudes, where they condensed to form the skirt clouds.  相似文献   
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