The potential of liposomes as a nutrient supplement in first-feeding marine fish larvae     

Koven  Barr  Hadas  Ben-Atia  Chen  Weiss  & Tandler 《Aquaculture Nutrition》1999,5(4):251-256

The ingestion rate (ng liposome larva^–1 h^–1) of extruded [1–¹⁴C] palmitic acid-labelled liposomes containing physiological saline (PHS) or cod fish extract (CFE), was tested in 5-day-old gilthead seabream Sparus aurata and white grouper Epinephelus aeneus larvae. A follow-up study compared the assimilation of radioactive free fatty acid (FFA) label of these two liposome treatments into six phospholipid and neutral lipid fractions as well as the nonlipid fraction in 5-day-old seabream. In seabream larvae, there was a 50-fold ( P  < 0.05) increase in the net consumption rate when fed CFE liposomes (2305.8 ng liposome larva^–1 h^–1) compared with liposomes containing physiological saline (42.7 ng liposome larva^–1 h^–1). A similarly significant ( P  < 0.05) but less marked pattern was also observed in the grouper larvae where the CFE treatment larvae ingested 238.5 ng liposome larva^–1 h^–1 compared with 54.3 ng liposome larva^–1 h^–1 in larvae fed the PHS liposomes. In seabream larvae ingesting CFE and PHS liposomes, radioactivity was found in all larval fractions analysed. However, marked treatment differences ( P  > 0.05) in assimilation were found only in the triacylglycerol fraction (3.4 and 0.6 dpm larva^–1 h^–1, respectively) and nonlipid fraction (11.2 and 15 dpm larva^–1 h^–1, respectively).  相似文献