首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   470篇
  免费   42篇
林业   19篇
农学   1篇
基础科学   1篇
  36篇
综合类   62篇
农作物   5篇
水产渔业   13篇
畜牧兽医   341篇
园艺   14篇
植物保护   20篇
  2023年   3篇
  2021年   6篇
  2020年   20篇
  2019年   9篇
  2018年   10篇
  2017年   11篇
  2016年   18篇
  2015年   11篇
  2014年   13篇
  2013年   29篇
  2012年   15篇
  2011年   9篇
  2010年   15篇
  2009年   23篇
  2008年   21篇
  2007年   11篇
  2006年   19篇
  2005年   23篇
  2004年   12篇
  2003年   7篇
  2002年   15篇
  2001年   10篇
  2000年   11篇
  1999年   16篇
  1998年   21篇
  1997年   13篇
  1996年   6篇
  1995年   5篇
  1994年   11篇
  1993年   10篇
  1992年   16篇
  1991年   13篇
  1990年   10篇
  1989年   6篇
  1988年   8篇
  1985年   3篇
  1984年   3篇
  1980年   2篇
  1979年   4篇
  1978年   3篇
  1976年   2篇
  1975年   2篇
  1974年   2篇
  1973年   2篇
  1972年   3篇
  1971年   6篇
  1969年   3篇
  1968年   2篇
  1967年   3篇
  1966年   2篇
排序方式: 共有512条查询结果,搜索用时 15 毫秒
91.
92.
93.
94.
Objective To study the potential role of an Australian corvid, the little raven (Corvus mellori), in the surveillance for exotic West Nile virus (WNV) in Australia. Method In a series of trials, little ravens were infected with WNV (strain 4132 New York 1999) and Kunjin virus (strain K42886) by the intramuscular route. They were observed for 20 days during which blood and swab samples were taken for virus isolation. Tissue samples were taken from ravens humanely killed during the acute infection period, and at the termination of the trials, for virus isolation, histopathology and immunohistochemistry. Results Ravens infected with WNV became mildly ill, but all recovered and seroconverted. Blood virus titres peaked around 3 to 4 days after inoculation at levels between 103.0 to 107.5 plaque forming units/mL. Virus or viral antigen was detected in spleen, liver, lung, kidney, intestine, testis and ovary by virus isolation and/or immunohistochemistry. WNV was detected in oral and cloacal swabs from 2 to 7 days post inoculation. The molecular and pathogenic characteristics of the inocula were consistent with them being of high virulence, as expected for this isolate. Ravens infected with Kunjin virus developed viraemia and seroconverted, although they did not develop disease. Conclusions Little ravens do not develop severe disease in response to virulent WNV infection and for this reason may not be important sentinel hosts in the event of an outbreak of WNV, as in North America. However, as they have relatively high viraemias, they may be able to support virus cycles.  相似文献   
95.
The aim of this study was to determine which cells are the source of production and target for leukotriene (LTs) action within the bovine ovary. Luteal (CL, days 14–16 of the oestrous cycle), steroidogenic cells (LSC) and endothelial cells (LEC) of the bovine corpus luteum (CL), and granulosa cells (GC) were isolated enzymatically, cultured in a monolayer and incubated with LTC4, LTB4, Azelastine (an antagonist of LTC4) or Dapsone (an antagonist of LTB4). Then cells were collected for determination of mRNA expression for LT receptors (LTRs) and 5‐lipoxygenase (5‐LO) by real time RT‐PCR, and media were collected for determination of prostaglandin (PG)E2, F, progesterone (P4; LSC only), endothelin‐1 (ET‐1; LEC only) and 17‐β oestradiol (E2; GC only). The greatest mRNA expression for LTR‐II and 5‐LO were found in LEC, whereas LTR‐I mRNA expression did not differ among cell types. The level of PGE2 increased after LTs treatment in each type of ovarian cell, excluding LTC4 treatment in LEC. The secretion of PGF was also increased by LTs, but decreased after LTB4 treatment of LSC. In GC cultures, both LTs stimulated E2 secretion; in LEC cultures, LTB4 stimulated whereas LTC4 inhibited P4 secretion; in LEC cultures, LTC4 stimulated but LTB4 inhibited ET‐1 secretion. The results show that LTs are produced locally and are involved in PGs production/secretion in all examined cells (LSC, LEC and GC) of bovine ovary. Leukotriene treatment modulate secretion of E2, by GC, P4 by LSC and ET‐1 by LEC, which indicates that LTs are involved in regulation of ovarian secretory functions.  相似文献   
96.
97.
98.
99.
A trial, at Spreyton in north-western Tasmania, studied the effects of Cytolin and NAA sprays on the thinning and shape of ‘Delicious’ apples. Untreated control and hand thinned trees were compared with trees treated with Cytolin or NAA alone, or in combination. Cytolin was applied at 25 mg 1~‘ at the king petal (KP) stage. NAA was applied at 8 mg I“1 at full bloom (FB) and 7,10, or 15 days after full bloom (AFB). Cytolin alone had no effect on thinning, fruit weight or on pip numbers but it did improve fruit shape. The combination of cytolin and NAA at FB overthinned. Cytolin and NAA combinations applied at all times after FB significantly decreased both mean fruit weight and pip numbers and reduced the percentage of fruit > 70 mm. All Cytolin-NAA combinations and the NAA treatments applied at 10 and 15 days AFB resulted in unacceptably high percentages of apples < 45 mm in diameter (pygmy fruit). It is recommended that Cytolin and NAA should not be used in the same spray programme. NAA alone is a most effective thinner at FB or seven days AFB.  相似文献   
100.
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号