Canine visceral leishmaniasis in urban and rural areas of Northeast Brazil     

Queiroz PV  Monteiro GR  Macedo VP  Rocha MA  Batista LM  Queiroz JW  Jerônimo SM  Ximenes MF 《Research in veterinary science》2009,86(2):267-273

The purpose of this study was to determine the clinical and laboratory profiles of canine leishmaniasis in two distinct areas. Dogs from urban and rural areas were examined. The population studied in the metropolitan area included 54 dogs. Of these, 20 (37%) animals did not present with any signs suggestive of visceral leishmaniasis (VL). Among these, only eight were confirmed negative by ELISA (rK39 and CE) and 12 dogs, clinically negative for leishmaniasis, were seropositive by ELISA (rK39 and CE). Thinness, conjunctivitis and onychogryphosis were the most frequent clinical signs in the urban areas, followed by crusty lesions, alopecia, ulcerated lesions, hyperkeratosis and exfoliation. In the metropolitan area human VL cases occurred mainly in 1991, 1992, 1999 and 2000. In the rural areas the ELISA rK39 test detected a seroprevalence of 11.3% and ELISA CE (Leishmania crude extract) of 20.6%. Thirty-nine dogs were examined 6 months after the first visit. Serological exams using rK39 antigen showed seroconversion of only one dog, whereas Leishmania CE showed seroconversion of 13 (33.4%) dogs. In this rural environment 83.3% of the positive dogs were asymptomatic. Lutzomyia intermedia and Lu. longipalpis were the most predominant sandfly vector species. Amastigotes were identified in spleen and liver fragments of symptomatic necropsied animals. PCR amplification of DNA isolated from promastigote culture indicated that the species was Leishmania chagasi. This finding suggests that delayed diagnosis and euthanasia of potentially infectious animals may occur with an increased transmission risk to sandflies and subsequently to humans.  相似文献   

The use of preserved equine renal capsule to repair lamellar corneal lesions in normal dogs   总被引：1，自引：0，他引：1  

Andrade AL  Laus JL  Figueiredo F  Batista CM 《Veterinary ophthalmology》1999,2(2):79-82

The purpose of this study was to evaluate the use of equine renal capsule preserved in 98% glycerine to repair lamellar corneal lesions in normal dogs. For this purpose, 12 dogs, divided into six groups ( n  = 2), were used to evaluate the 1st to 7th day, 15th day and 30th to 60th postoperative day. In order to perform the histologic study, the clinical procedures were analyzed, while the recipient's corneas were collected. The photophobia and blepharospasm also were more intense in the 1st to 7th postoperative day, and regressed in the 15th postoperative day. Therefore, the edema and the vascular events were both more frequent in the intermediary phases and regressed in the late periods. On the other hand, the morphological evaluation demonstrated an inflamatory exudate, also in the intermediary and late periods. These results suggested that the equine renal preserved capsule could be a useful alternative tissue to repair lamellar corneal lesions in dogs.  相似文献   

Canine visceral leishmaniasis in São José de Ribamar, Maranhão State, Brazil     

Guimarães KS  Batista ZS  Dias EL  Guerra RM  Costa AD  Oliveira AS  Calabrese KS  Cardoso FO  Souza CS  do Vale TZ  Gonçalves da Costa SC  Abreu-Silva AL 《Veterinary parasitology》2005,131(3-4):305-309

Here, we describe the situation of canine visceral leishmaniasis in two villages of S?o José de Ribamar in Maranh?o State/Brazil, where human cases have been registered. Blood samples of 36 household crossbred dogs from Sergio Tamer village and 43 dogs from Quinta village were collected and the serum used for serological diagnosis. An Indirect Fluorescent Antibody Test (IFAT) and enzyme-linked immunosorbent assay (ELISA) were used to detect antibodies against Leishmania. The clinical examination showed that 25% of the canine population of Quinta presented a poor body condition and in 39%, ectoparasites (ticks and fleas) were detected. In both tests, serology revealed that 21% (9 out of 43) of the dogs presented antibodies against Leishmania (55% were asymptomatic and 45% were symptomatic). In the Vila Sérgio Tamer, 25% (9 out of 36) of the dogs were seropositive for Leishmania (66.67% were asymptomatic and 33.33% were symptomatic), 33% presented poor body condition, and 22% have ectoparasites. The clinical signs more frequent were skin lesions. The statistical analysis showed that there was no statistical difference (p>0.05) between the seropositivity of the dogs from the two villages. The same was observed when the clinical signs were compared (p>0.05). Both villages have favorable conditions to maintain the cycle of leishmaniasis.  相似文献   

Virological and immunological responses to porcine reproductive and respiratory syndrome virus in a large population of gilts   总被引：7，自引：0，他引：7       下载免费PDF全文

Laura Batista  Carlos Pijoan  Scott Dee  Michael Olin  Thomas Molitor  Han Soo Joo  Zhenguo Xiao    Michael Murtaugh 《Canadian journal of veterinary research》2004,68(4):267-273

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Structural changes in the testes and epididymides of bucks 16 weeks after bilateral vasectomy     

Batista M  Calero P  Rodriguez F  González F  Cabrera F  Gracia A 《The Veterinary record》2002,151(24):740-741

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Response of juvenile pacu (Piaractus mesopotamicus Holmberg, 1887) to balanced digestible protein     

Kifayat Ullah Khan  Robert Mervyn Gous  Cleber Fernando M. Mansano  Thiago Matias T. Nascimento  Rafael de S. Romaneli  Andressa Tellechea Rodrigues  Nilva Kazue Sakomura  Joo Batista K. Fernandes 《Aquaculture Research》2020,51(9):3729-3740

This 45‐day work aimed to determine the response of juvenile pacu (Piaractus mesopotamicus) to balanced digestible protein (BDP) and to use these responses to determine whether the optimum economic levels of BDP would differ depending on the form in which the fish is sold. Six isoenergetic diets containing 163, 201, 238, 272, 315 and 348 g/kg BDP (dry‐matter basis) were prepared through the serial dilutions of a high‐protein diet with the low‐protein diet. Fish (initial average body weight, 10.82 ± 0.14 g) were fed with respective experimental diets three times a day until apparent satiation. The optimum biological level of BDP was calculated as 326 g/kg (dry‐matter basis) by the quadratic regression model for maximum body weight gain of juvenile pacu. To maximize economic returns (US$/kg) for different end products, the optimum economic levels of BDP were calculated as 311, 317 and 319 g/kg (dry‐matter basis) by an economic model for whole‐body, eviscerated and sliced juvenile pacu respectively. This finding revealed that obtaining maximum biological performance of fish in the case of high feed costs or low prices of the end product in consumer market would substantially reduce the economic returns.  相似文献   

Evaluation of systems for reducing the transmission of Porcine reproductive and respiratory syndrome virus by aerosol   总被引：1，自引：0，他引：1       下载免费PDF全文

Scott A. Dee  Laura Batista  John Deen    Carlos Pijoan 《Canadian journal of veterinary research》2006,70(1):28-33

The purpose of this study was to compare 3 methods for the reduction of aerosol transmission of Porcine reproductive and respiratory syndrome virus (PRRSV): high-efficiency particulate air (HEPA) filtration, low-cost filtration, and ultraviolet light (UV) irradiation. The HEPA-filtration system involved a pre-filter screen, a bag filter (EU8 rating), and a HEPA filter (EU13 rating). The low-cost-filtration system contained mosquito netting (pre-filter), a fiberglass furnace filter, and an electrostatic furnace filter. For UV irradiation, a lamp emitted UVC radiation at 253.7 nm. No form of intervention was used in the control group. The experimental facilities consisted of 2 chambers connected by a 1.3-m-long duct. Recipient pigs, housed in chamber 2, were exposed to artificial aerosols created by a mechanically operated mister containing modified live PRRSV vaccine located in chamber 1. Aerosol transmission of PRRSV occurred in 9 of the 10 control replicates, 8 of the 10 UVC-irradiation replicates, 4 of the 10 low-cost-filtration replicates, and 0 of the 10 HEPA-filtration replicates. When compared with no intervention, HEPA filtration and low-cost filtration significantly reduced PRRSV transmission (P < 0.0005 and = 0.0286, respectively), whereas UV irradiation had no effect (P = 0.5). However, low-cost filtration and UV irradiation were significantly less effective (P = 0.043 and P < 0.0005, respectively) than HEPA filtration. In conclusion, under the conditions of this study, HEPA filtration was significantly more effective at reducing aerosol transmission of PRRSV than the other methods evaluated.  相似文献   

Further evaluation of alternative air-filtration systems for reducing the transmission of Porcine reproductive and respiratory syndrome virus by aerosol          下载免费PDF全文

Scott A. Dee  John Deen  Jean Paul Cano  Laura Batista    Carlos Pijoan 《Canadian journal of veterinary research》2006,70(3):168-175

The purpose of this study was to compare 4 methods for the reduction of aerosol transmission of Porcine reproductive and respiratory syndrome virus (PRRSV): high-efficiency particulate air (HEPA) filtration, 2x-low-cost filtration, bag filtration, and use of a filter tested against particles derived from dioctylphthalate (DOP). The HEPA-filtration system used a prefilter screen, a bag filter (Eurovent [EU] 8 rating), and a HEPA filter (EU13 rating). The low-cost-filtration system contained mosquito netting (prefilter), 2 fiberglass furnace filters, and 2 electrostatic furnace filters. Bag filtration involved the use of a filter rated EU8 and a minimum efficiency reporting value (MERV) of 14. The 95%-DOP, 0.3-microm-filtration system involved a pleat-in-pleat V-bank disposable filter with a 95% efficiency rating for particles 0.3 microm or greater in diameter and ratings of EU9 and MERV 15. No form of intervention was used in the control group. The experimental facilities consisted of 2 chambers connected by a 1.3-m-long duct containing the treatments. Recipient pigs, housed in chamber 2, were exposed to artificial aerosols created by a mechanically operated mister containing modified live PRRSV vaccine located in chamber 1. Aerosol transmission of PRRSV occurred in 0 of the 10 HEPA-filtration replicates, 2 of the 10 bag-filtration replicates, 4 of the 10 low-cost-filtration replicates, 0 of the 10 95%-DOP, 0.3-microm-filtration replicates, and all 10 of the control replicates. Using a similar approach, we further evaluated the HEPA- and 95%-DOP, 0.3-microm-filtration systems. Infection was not observed in any of the 76 HEPA-filtration replicates but was observed in 2 of the 76 95%-DOP, 0.3-microm replicates and 42 of the 50 control replicates. Although the difference between the 95%-DOP, 0.3-microm and control replicates was significant (P < 0.0005), so was the level of failure of the 95%-DOP, 0.3-microm system (P = 0.02). In conclusion, under the conditions of this study, some methods of air filtration were significantly better than others in reducing aerosol transmission of PRRSV, and HEPA filtration was the only system that completely prevented transmission.  相似文献   

Influence of the Freezing Technique (Nitrogen Liquid vs Ultrafreezer of −152°C) and Male-to-Male Variation Over the Semen Quality in Canarian Mastiff Breed Dogs     

M Batista  D Alamo  F González  MG Cruz  A Gracia 《Reproduction in domestic animals》2006,41(5):423-428

This study was carried out to assess the in vitro quality of canine semen frozen in an ultrafreezer at -152 degrees C and to evaluate the male-to-male variation of frozen semen in five male dogs of the Canarian Mastiff breed. Four ejaculates of each dog were processed individually (5% glycerol and 0.5% Equex) to reach a final concentration of 100 x 10(6) spermatozoa/ml. Then, two freezing techniques were tested to assess the seminal quality (sperm motility, live spermatozoa and abnormal sperm cell percentages) at 1, 30, 60, 120 and 360 days after freezing: (i) semen was frozen and stored in liquid nitrogen; (ii) semen was frozen and stored in the ultrafreezer at -152 degrees C. After freezing-thawing, both freezing protocols showed no significant differences in sperm motility and the percentages of live and abnormal spermatozoa. On the other hand, the microscopic characteristics of spermatozoa in fresh semen were practically similar among males; however, after the semen processing and freezing, significant differences were observed (p < 0.05) among males, especially as regards sperm motility. This inter-individual variability was detected in both freezing protocols, showing that the male-to-male variation in the seminal quality post-freezing was independent of the freezing technique used. The in vitro results obtained in the Canarian Mastiff breed confirmed that the use of ultra-freezers at -152 degrees C is a potential alternative to liquid nitrogen for storing canine semen for long periods of time.  相似文献   

OC13Induction of Parturition in Canary Goats with Luprostiol     

M Batista  T Niño  D Alamo  F Cabrera  N Castro 《Reproduction in domestic animals》2006,41(S2):106-106

Twenty-two pregnant goats of the Canarian breed were administered 10 mg of the prostaglandin analogue luprostiol intramuscular (IM) on day 145 of pregnancy; seventeen pregnant goats were injected IM with saline solution the same day. Twenty hours after luprostiol application, goats were continuously observed in order to record the following parameters: parturition, dystocia incidence, placental delivery and live births. Parturition was successfully induced in all goats; the mean interval to parturition (first kid delivery) was of 32.9 ± 1.0 (SEM) hours (h) and the range varied from 21 to 41 h; in addition, no significant differences (p > 0.1) were detected between nuliparous and multiparous goats (31.3 ± 1.3 h and 34.7 ± 1.2 h, respectively). In the control group, the interval from saline injection to parturition was 75.9 ± 12.5 h (mean ± SEM), showing a range between 34 and 138 h. All the goats expelled the fetal membranes in the first 6 h after the induced or natural parturition. The incidence of dystocia due to fetal maldisposition was not significantly different between induced and control goats (9.0% and 11.8%, respectively). In addition, the percentage of live kids was practically similar between both groups (90.7% and 88.0%, induced vs control goats). This study confirms the effectiveness of the luprostiol to induce the parturition in goats, showing a narrow range (30–37 h) in most of the induced females (86.3%; 19/22 goats). The administration of prostaglandin analogue is a basic tool to plan kidding, allowing a effective concentration of effort and resources at the time of parturition.  相似文献