Characterization of an ecto-5'-nucleotidase activity present on the cell surface of Tritrichomonas foetus     

De Jesus JB  Cosentino-Gomes D  Meyer-Fernandes JR 《Veterinary parasitology》2011,179(1-3):50-56

Tritrichomonas foetus is the causative agent of sexually transmitted trichomoniasis in cattle. In females, the infection can be associated with infertility, vaginitis, endometritis, abortion or pyometra, leading to significant economic losses in cattle raising. T. foetus is devoid of the ability to synthesize purine nucleotides de novo, depending instead on salvaging purines from the host environment. Ecto-5'-nucleotidase catalyzes the final step of extracellular nucleotide degradation, the hydrolysis of nucleoside 5'-monophosphates to the corresponding nucleosides and Pi. In this work we show that living, intact cells of T. foetus were able to hydrolyze 5'AMP at a rate of 12.57 ± 1.23 nmol Pi × h(-1) × 10(-7) cells at pH 7.2 and the 5'AMP hydrolysis is due to a plasma membrane-bound ecto-enzyme activity. The apparent K(m) for 5'AMP was 0.49 ± 0.06 mM. In addition to 5'AMP, the enzyme hydrolyzed all substrate monophosphates tested except 3'AMP. No divalent metals or metal chelators were able to modulate enzyme activity. Phosphatase inhibitors did not have an effect on ecto-5'-nucleotidase activity while ammonium molybdate did inhibit the activity in a dose dependent manner. The presence of adenosine in the culture medium negatively modulated the enzyme. These results indicate the existence of an ecto-5'-nucleotidase that may play a role in the salvage of purines.  相似文献   

In vitro and in vivo evaluation of ram sperm frozen in tris egg-yolk and supplemented with superoxide dismutase and reduced glutathione     

Silva SV  Soares AT  Batista AM  Almeida FC  Nunes JF  Peixoto CA  Guerra MM 《Reproduction in domestic animals》2011,46(5):874-881

The aim of the present study was to evaluate the in vitro and in vivo effect of the addition of superoxide dismutase (SOD) and reduced glutathione (GSH) to ram semen freezing extender. Significant differences (p < 0.05) were detected between groups regarding total motility (TM), straightness (STR) and wobble (WOB), for which the GSH 7 mM group had lesser TM and better STR than the other groups and the GSH 5 and 7 mM groups had higher wobble values than the control, SOD 25 and 100 U/ml groups. The ultrastructural analysis revealed that the acrosome was better preserved after freezing in the SOD 100 U/ml and GSH 2 and 5 mM (p < 0.05) groups than the other groups, whereas mitochondria in both the control group and the 7 mM GSH group suffered the greatest damage. The plasma membrane remained preserved after freezing, regardless of the group. For in vivo fertilization, the SOD group achieved better results than the GSH group (p > 0.05). It can therefore be concluded that the addition of SOD 100 U/ml and GSH 2 and 5 mM preserves the acrosome integrity of frozen ram spermatozoa, while the addition of SOD 100 U/ml to Tris egg-yolk extender offers protection to the membranes of sperm cells after thawing.  相似文献   

Economic and financial model to the mass-rearing of Macrolophus pygmaeus (Rambur) (Heteroptera: Miridae), a biological control agent against the tomato moth Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) in protected culture     

Tiago Mota Dutra  Maria da Graça Batista  João CA Teixeira  Silvia Todorova  Luísa Oliveira  João Tavares  Isabel Borges  António Onofre Soares 《Pest management science》2023,79(10):3712-3720

BACKGROUND

Tuta absoluta (Meyrick) is a major pest of tomato produced in glasshouses and open field, causing severe damages to crops, reducing the quality of tomato fruits. The current maintenance of the pest populations below the economic threshold is not achieved by natural and classical control, thus requiring the continuous application of biological control agents (BCAs), under an augmentative or inoculative approach. The present study aims to develop an economic and financial model to evaluate the commercial viability of a continuous mass production of Macrolophus pygmaeus (Rambur), a BCA commonly used against the tomato moth, Tuta absoluta, in protected culture. The estimations for our model were based on two approaches: the farm-level impact analysis and the benefit–cost analysis.

RESULTS

The results of the farm-level analysis show that the adoption of a more sustainable biological control approach is profitable for farmers and the benefit–cost analysis provides evidence that the investment on a new factory dedicated to the mass rearing of M. pygmaeus to control tomato moth populations generates a positive net present value (NPV) of 7.2 million euros, corresponding to an internal rate of return (IRR) of 28.4% per year.

CONCLUSION

Our results are in line with (i) the more recent European Commission proposals for a new Regulation on sustainable use of plant protection products, which includes the reduction of 50% the use and risk of chemical pesticides by 2030 and (ii) most of the existing literature which conclude that new projects on BCA production are worth investments. © 2023 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.  相似文献   

Effects of bovine leukemia virus infection on milk neutrophil function and the milk lymphocyte profile     

Alice Maria Melville Paiva Della Libera  Fernando Nogueira de Souza  Camila Freitas Batista  Bruna Parapinski Santos  Luis Fernando Fernandes de Azevedo  Eduardo Milton Ramos Sanchez  Soraia Araújo Diniz  Marcos Xavier Silva  Jo?o Paulo Haddad  Maiara Garcia Blagitz 《Veterinary research》2015,46(1)

The effects of bovine leukemia virus (BLV) on the immune response have been extensively investigated; however, its effects on mammary gland immunity are only speculative. Although BLV has a tropism for B cells, it can affect both adaptive and innate immunities because these systems share many effector mechanisms. This scenario is the basis of this investigation of the effects of BLV on mammary gland immunity, which is largely dependent upon neutrophilic functions. Thus, the present study sought to examine neutrophilic functions and the lymphocyte profile in the milk of naturally BLV-infected cows. The viability of the milk neutrophils and the percentage of milk neutrophils that produced reactive oxygen species (ROS) or phagocytosed Staphylococcus aureus were similar between BLV-infected and BLV-uninfected dairy cows. Furthermore, the expression of CD62L and CD11b by the milk neutrophils and the percentage of milk neutrophils (CH138⁺ cells) that were obtained from the udder quarters of the BLV-infected cows were not altered. Conversely, the median fluorescence intensity (MFI) representing intracellular ROS production and the phagocytosis of S. aureus, the expression of CD44 by the milk neutrophils and the percentage of apoptotic B cells were lower in the milk cells from BLV-infected dairy cows, particularly those from animals with persistent lymphocytosis (PL). The lymphocyte subsets were not different among the groups, with the exception of the percentage of CD5⁻/CD11b⁻ B cells, which was higher in the milk cells from BLV-infected cows, particularly those with PL. Thus, the present study provides novel insight into the implications of BLV infection for mammary gland immunity.  相似文献   

Pseudopestalotiopsis elaeidis associated with leaf spots on Cycas revoluta in Brazil     

André Wilson Campos Rosado  Alessandra de Jesus Boari  Danilo Oliveira Ramos  Fábio Alex Custódio  Ayane Fernanda Ferreira Quadros  Izabel Cristina Alves Batista  Olinto Liparini Pereira 《Forest Pathology》2023,53(2):e12808

Cycas revoluta (Cycadaceae) is commonly cultivated as an ornamental tree in several countries. In April 2015, C. revoluta leaves showing brownish spots with a yellow halo that progressed to leaflet necrosis were observed in Belém, Pará state, Brazil. This study aimed to determine the aetiology of this disease on sago palm in Brazil. Based on DNA sequence-based phylogeny, morphology, and colony characterization, the fungus was identified as Pseudopestalotiopsis elaeidis. To the best of our knowledge, this is the first report of P. elaeidis associated with leaf spots on C. revoluta worldwide.  相似文献   

Vascular Endothelial (VEGF) and Epithelial Growth Factor (EGF) as Well as Platelet‐Activating Factor (PAF) and Receptors are Expressed in the Early Pregnant Canine Uterus     

S Schäfer‐Somi  S Sabitzer  D Klein  E Reinbacher  H Kanca  HB Beceriklisoy  OA Aksoy  I Kucukaslan  HC Macun  S Aslan 《Reproduction in domestic animals》2013,48(1):20-26

The aim of this study was to investigate the course of expression of platelet‐activating factor (PAF), PAF‐receptor (PAF‐R), epidermal growth factor (EGF), EGF‐R, vascular endothelial growth factor (VEGF), VEGF‐R1 and VEGF‐R2 in uterine tissue during canine pregnancy. For this purpose, 20 bitches were ovariohysterectomized at days 10–12 (n = 10), 18–25 (n = 5) and 28–45 (n = 5) days after mating, respectively. The pre‐implantation group was proven pregnant by embryo flushing of the uterus after the operation, the others by sonography. Five embryo negative, that is, non‐pregnant, bitches in diestrus (day 10–12) served as controls. Tissue samples from the uterus (placentation sites and horn width, respectively) were excised and snap‐frozen in liquid nitrogen after embedding in Tissue Tec^®. Extraction of mRNA for RT‐PCR was performed with Tri‐Reagent. In the embryos, mRNA from all factors except VEGF was detected. In the course of pregnancy, significantly higher expression of PAF and PAFR as well as VEGF and VEGFR2 during the pre‐implantation stage than in all other stages and a strong upregulation of EGF during implantation were characteristic. The course of EGF was in diametrical opposition to the course of the receptor. These results point towards an increased demand for VEGF, EGF and PAF during the earliest stages of canine pregnancy.  相似文献   

Suture exostosis causing obstruction of the nasolacrimal duct in three horses     

HB Carslake 《New Zealand veterinary journal》2013,61(4):229-234

CASE HISTORY Three horses were presented with facial swelling and epiphora.

CLINICAL FINDINGS: All horses had facial swellings and radiographic findings consistent with exostosis at the lacrimomaxillary suture, and ipsilateral epiphora. Positive contrast dacryocystorhinography demonstrated that the site of obstruction of the nasolacrimal duct was located where the duct traverses the lacrimomaxillary suture.

DIAGNOSIS: Lacrimomaxillary suture exostosis.

CLINICAL RELEVANCE: Lacrimomaxillary suture exostosis should be considered as a differential diagnosis in cases of epiphora in horses. Dacryocystorhinography, preferably retrograde and normograde, may be used as an aid to diagnosis.  相似文献   

Nitrite toxicity to Litopenaeus schmitti (Burkenroad, 1936, Crustacea) at different salinity levels          下载免费PDF全文

Edison Barbieri  Ana Cristina Vigliar Bondioli  Camila Batista de Melo  Marcelo Barbosa Henriques 《Aquaculture Research》2016,47(4):1260-1268

Litopenaeus schmitti juveniles (total length 15 ± 0.4 cm) were exposed to different concentrations of nitrite using the static renewal method at different salinity levels (5‰, 20‰ and 35‰) at pH 8.0 and 20 °C. The 24, 48, 72 and 96 h LC50 values of nitrite in L. schmitti juveniles were 40.72, 32.63, 24.63, and 19.12 mg L⁻¹ at 5‰; 53.52, 38.60, 27.76, 25.55 mg L⁻¹ at 20‰; 54.32, 47.87, 41.67 and 38.88 mg L⁻¹ at 35‰ salinity. As the salinity decreased from 35‰ to 5‰, susceptibility to nitrite increased by 33.4%, 46.7%, 69.2% and 103.3%, after 24, 48, 72 and 96 h of exposure respectively. Furthermore, we found that exposure of shrimp to nitrite caused an increase in oxygen consumption by 137.3%, 99.2% and 81.4% and an increase in the ammonia excretion level by 112.5%, 87% and 64.3% with respect to the control with decreasing salinity levels.  相似文献   

Growth and fatty acid composition of juvenile Cerastoderma edule (L.) fed live microalgae diets with different fatty acid profiles     

I. Reis Batista  P. Kamermans  M.C.J. Verdegem  A.C. Smaal 《Aquaculture Nutrition》2014,20(2):132-142

The importance of dietary 20:5n‐3 (EPA), 22:6n‐3 (DHA) and 20:4n‐6 (ARA) for growth, survival and fatty acid composition of juvenile cockles (Cerastoderma edule) was investigated. Cockles of 6.24 ± 0.04 mm and 66.14 ± 0.34 mg (live weight) were distributed into three treatments where live microalgae diets were fed constantly below the pseudofaeces production threshold, for three weeks. Diets had distinct fatty acid profiles: high EPA (53% Chaetoceros muelleri + 47% Pyramimonas parkeae), no DHA (47% Brachiomonas submarina + 53% Tetraselmis suecica) and low ARA concentrations (73% P. parkeae + 27% Phaeodactylum tricornutum). Growth was positively affected by high EPA and low ARA diets, whereas no significant growth was observed for the no DHA diet. High mortality of cockles fed no DHA diet raises questions about its suitability for cockles. In balanced diets with EPA and DHA, lower concentrations of ARA do not limit growth. The impact of dietary fatty acids was evident in the fatty acids of neutral and polar lipids of cockles. In polar lipids of all cockles, there was a decrease in EPA, in contrast to an increase in DHA. The combination of EPA and DHA in a live microalgae diet was beneficial for the growth and survival of juvenile cockles.  相似文献   

Preference and infestation level of Anastrepha fraterculus (Wiedemann, 1830) on fruits of some Psidium guajava L. cultivars and relation to their physicochemical parameters     

Flávia Q. de Oliveira  Arlindo L. Boiça Junior  Maria de Lourdes Z. Costa  Patricia A. Sanches  Julio M. M. Walder  Jacinto de L. Batista 《Phytoparasitica》2014,42(4):475-483

This study aimed to verify the preference and infestation level of Anastrepha fraterculus (Diptera: Tephritidae) [South American fruit fly] in fruits of guava cultivars and to correlate them to variables such as peel coloration, soluble solids and pH of fruit cultivars. The following cultivars were used: Pedro Sato, Paluma, Cascão and Século XXI. The infestation was evaluated in cages, considering two scenarios: no-choice and multiple choice. In both tests, evaluations of the fruit attraction to insects were conducted for a period of 1′, 3′, 5′, 10′, 20′, 30′, 1 h, 2 h, 6 h, 12 h and 24 h. The visit of A. fraterculus on the assayed cultivars in relation to the time was studied by logistic regression. After 10 days, the number of larvae in each fruit was recorded. In the multiple choice test, the visit proportions were significantly higher in the fruits of cvs. Século XXI and Pedro Sato than in those of cvs. Paluma and Cascão. In the no-choice test, the visit proportions were significantly lower in the Paluma fruits. In both tests, the rate of fruit infestation by A. fraterculus did not differ among cvs. Pedro Sato, Paluma and Cascão, whereas the fruits of cv. Século XXI were more infested. The indexes of pH did not interfere with the infestation of A. fraterculus, whereas a high rate of soluble solids and low color angle appear to be crucial for discriminating the fruits of the most susceptible cultivars. Infestation rate of Século XXI fruits displayed significant correlations with: °Brix (r= 0.7078) and color angle (h) (r= –0.9499) of guava fruits under the multiple choice conditions.  相似文献