Statement on scientific publication and security     

Atlas R  Campbell P  Cozzarelli NR  Curfman G  Enquist L  Fink G  Flanagin A  Fletcher J  George E  Hammes G  Heyman D  Inglesby T  Kaplan S  Kennedy D  Krug J  Levinson R  Marcus E  Metzger H  Morse SS  O'Brien A  Onderdonk A  Poste G  Renault B  Rich R  Rosengard A  Salzburg S  Scanlan M  Shenk T  Tabor H  Varmus H  Wimmer E  Yamamoto K;Journal Editors  Authors Group 《Science (New York, N.Y.)》2003,299(5610):1149

  相似文献   

Public health. A sound rationale needed for phase III HIV-1 vaccine trials     

Burton DR  Desrosiers RC  Doms RW  Feinberg MB  Gallo RC  Hahn B  Hoxie JA  Hunter E  Korber B  Landay A  Lederman MM  Lieberman J  McCune JM  Moore JP  Nathanson N  Picker L  Richman D  Rinaldo C  Stevenson M  Watkins DI  Wolinsky SM  Zack JA 《Science (New York, N.Y.)》2004,303(5656):316

  相似文献   

Aesthetic preferences of non-farmers and farmers for different land-use types and proportions of ecological compensation areas in the Swiss lowlands   总被引：1，自引：0，他引：1  

Xenia Junge  Petra Lindemann-Matthies  Marcel Hunziker  Beatrice Schüpbach 《Biological conservation》2011,(5):1430-1440

Beyond its traditional function of food production, agricultural land offers public amenities such as the protection of natural resources and landscape scenery. This study investigates the preferences of non-farmers and farmers for nine landscape scenarios in the Swiss lowlands. The nine landscapes were the result of a photo editing process combining three land-use types (arable crops, grassland and a mixture of both) and three proportions of ecological compensation areas (0%, 10% and 30%). The landscape photographs were randomly arranged on one page of a paper-based questionnaire which was sent to a random sample of 4000 Swiss households (non-farmers) and 500 farmers. The respondents (1376 non-farmers and 276 farmers) rated each landscape by attractiveness. Both non-farmers and farmers preferred a mixed land-use type or one dominated by arable crops over one dominated by grassland. Non-farmers’ preference ratings were highly influenced by the proportion of ecological compensation areas (ECAs) in the rated landscape: Non-farmers rated a landscape with a mixed land-use type and 30% ECAs highest, whereas farmers rated a landscape dominated by arable crops and 10% ECAs highest. The results indicate that heterogeneous landscapes (mixed land use, high proportion of ECAs) influence scenic beauty positively. Thus, farming practices and agro-environment schemes such as ECAs can have an impact on the visual attractiveness of a landscape.  相似文献   

The newly proposed EU risk assessment concept for the sediment compartment     

Caroline Riedhammer  Beatrice Schwarz-Schulz 《Journal of Soils and Sediments》2001,1(2):105-110

Within the frame of the EU Existing Chemicals Regulation (793/93/EEC), a new concept for the assessment of the sediment compartment has been developed. During the assessment process it has become clear that for substances that strongly adsorb or bind to sediment the equilibrium partitioning method (EPM) presently employed is not sufficient. The new approach is therefore based on long-term sediment tests with benthic organisms. A testing strategy has been developed which considers species which represent different habitats and feeding strategies in order to adequately address all possible routes of exposure. The new concept has proven to be valuable in risk assessments, two examples (3,4-DCA, DODMAC) of which are shown in more detail.  相似文献   

Sexual dimorphism of the kidney in the NMRI mouse as shown by Dolichos biflorus agglutinin labelling     

Johanna  Plendl  Barbara  Schoenlejber  W. Schmahl    A. Beatrice  Murray F. Sinowatz 《Anatomia, histologia, embryologia》1992,21(2):118-126

The histological affinity pattern of Dolichos biflorus agglutinin (DBA) in kidneys from mice (NMRI, Balb/c, CBA) and rats (Wistar) fixed by perfusion with formalin, Bouin, or HgCl2 was investigated with a horseradish peroxidase conjugate. The animals were examined from fetal stage to adulthood. Adult female NMRI mice exhibited constant DBA labelling, with DBA binding to cells of the proximal and collecting tubules. Moreover the vascular endothelium of the renal papilla was found to be DBA-positive in 50% of adult female animals. In contrast, there was only very little DBA binding in the kidneys of male adult NMRI mice. There was no sexual dimorphism in lectin labelling in kidneys from other strains of mice or from rats.  相似文献   

Validity and reliability of the Block98 food-frequency questionnaire in a sample of Canadian women     

Boucher B  Cotterchio M  Kreiger N  Nadalin V  Block T  Block G 《Public health nutrition》2006,9(1):84-93

OBJECTIVE: To assess the validity and reliability of the most recent adaptation of Block's full-diet food-frequency questionnaire (FFQ) among a sample of Canadian women. DESIGN: Participants completed a self-administered FFQ (FFQ1), two unannounced 24-hour recalls (weekday and weekend) and a second FFQ (FFQ2) between October 2003 and February 2004. FFQs and recalls were analysed for 32 nutrients using Block Dietary Data Systems and the University of Minnesota's Nutrient Data System. Mean and median intakes were computed, along with crude and deattenuated Pearson correlation coefficients between FFQ1 and the average of two recalls (validity) and between FFQ1 and FFQ2 (reliability). SETTING: Ontario, Canada. SUBJECTS: A random population-based sample (n = 166) of women aged 25 to 74 years. RESULTS: One hundred and fifteen (69%) women completed FFQ1, 96 completed FFQ1 and both recalls, and 93 completed both FFQs, about 56 days apart. Mean intakes were similar for most nutrients. FFQ reliability was high, with Pearson correlation coefficients having a median of 0.75, ranging from 0.57 to 0.90 (macronutrients) and from 0.65 to 0.88 (micronutrients from supplements and food). FFQ validity was moderate to high, with deattenuated Pearson correlation coefficients having a median of 0.59, ranging from 0.11 to 0.73 (macronutrients) and from 0.50 to 0.76 (micronutrients from supplements and food). Our micronutrient correlations were similar to or higher than those of other studies that included supplements. Two correlations <0.40 were associated with fats. CONCLUSIONS: The validity and reliability of this full-diet version of the Block FFQ were moderate to high, supporting its use in future studies among Canadian women.  相似文献   

Genomic characterization of H1N2 swine influenza viruses in Italy     

Moreno A  Chiapponi C  Boniotti MB  Sozzi E  Foni E  Barbieri I  Zanoni MG  Faccini S  Lelli D  Cordioli P 《Veterinary microbiology》2012,156(3-4):265-276

Three subtypes (H1N1, H1N2, and H3N2) are currently diffused worldwide in pigs. The H1N2 subtype was detected for the first time in Italian pigs in 1998. To investigate the genetic characteristics and the molecular evolution of this subtype in Italy, we conducted a phylogenetic analysis of whole genome sequences of 26 strains isolated from 1998 to 2010. Phylogenetic analysis of HA and NA genes showed differences between the older (1998-2003) and the more recent strains (2003-2010). The older isolates were closely related to the established European H1N2 lineage, whereas the more recent isolates possessed a different NA deriving from recent human H3N2 viruses. Two other reassortant H1N2 strains have been detected: A/sw/It/22530/02 has the HA gene that is closely related to H1N1 viruses; A/sw/It/58769/10 is an uncommon strain with an HA that is closely related to H1N1 and an NA similar to H3N2 SIVs. Amino acid analysis revealed interesting features: a deletion of two amino acids (146-147) in the HA gene of the recent isolates and two strains isolated in 1998; the presence of the uncommon aa change (N66S), in the PB1-F2 protein in strains isolated from 2009 to 2010, which is said to have contributed to the increased virulence. These results demonstrate the importance of pigs as mixing vessels for animal and human influenza and show the presence and establishment of reassortant strains involving human viruses in pigs in Italy. These findings also highlighted different genomic characteristics of the NA gene the recent Italian strains compared to circulating European viruses.  相似文献   

Association of wild bird density and farm management factors with the prevalence of E. coli O157 in dairy herds in Ohio (2007-2009)     

Cernicchiaro N  Pearl DL  McEwen SA  Harpster L  Homan HJ  Linz GM  Lejeune JT 《Zoonoses and public health》2012,59(5):320-329

Our objective was to determine the role that European starlings (Sturnus vulgaris) play in the epidemiology of Escherichia coli O157:H7 in dairy cattle. We visited 150 dairy farms in Ohio twice during summer and fall months from 2007 to 2009. Fresh faecal pats from 30 lactating cows were collected during each visit. Information on farm management and environmental variables was gathered through a questionnaire administered to the farm owner. The number of starlings observed on the farm was also recorded. Approximately 1% of dairy cattle and 24% of farms were positive for E. coli O157. Risk factors associated with the presence of E. coli O157 in faecal pats included contact between adult cattle and calves, types or number of ventilation and manure management systems and number of birds per milking cow.  相似文献   

Coxofemoral joint radiography in standing cattle     

Wenzinger B  Hagen R  Schmid T  Nuss K 《Veterinary radiology & ultrasound》2012,53(4):424-429

The objective of this study was to establish a technique for radiographic examination of the coxofemoral joint and adjacent bony structures in standing cattle. Left (or right) 30° dorsal-right (or left) ventral radiographic views of the coxofemoral joint region of standing cattle (n = 10) with hind limb lameness were evaluated retrospectively. In addition, an experimental study of oblique laterolateral views of the coxofemoral joint region of a bovine skeleton at angles of 15-45° was carried out to determine the optimal position for visualization of the hip region. In the 10 clinical patients, the bodies of the ilium and ischium, the acetabulum and proximal third of the femur could be assessed. Six of these cattle had fractures of the body of the ilium and body of the ischium, five with and one without involvement of the acetabulum, two had craniodorsal and one caudoventral luxation of the femur and one had a femoral neck fracture. The described laterodorsal-lateroventral radiographs of the hip region in standing cattle were suitable for assessing the coxofemoral joint, the proximal aspect of the femur and parts of the ischium, ilium and pubis. After testing the optimal angle on the skeleton, it was seen that distortion and superimposition were minimized by positioning the X-ray beam at an angle of 25° to the horizontal plane. It can be concluded that the described technique improves the evaluation of injuries of the coxofemoral region in cattle. With the appropriate angle, the technique can also be applied in recumbent cattle.  相似文献   

Description of a new procedure to estimate the carbon stocks of all forest pools and impact assessment of methodological choices on the estimates     

Nicolas Latte  Gilles Colinet  Adeline Fayolle  Philippe Lejeune  Jacques Hébert  Hugues Claessens  Sébastien Bauwens 《European Journal of Forest Research》2013,132(4):565-577

Forest ecosystems play a major role in atmospheric carbon sequestration and emission. Comparable organic carbon stock estimates at temporal and spatial scales for all forest pools are needed for scientific investigations and political purposes. Therefore, we developed a new carbon stock (CS) estimation procedure that combines forest inventory and soil and litter geodatabases at a regional scale (southern Belgium). This procedure can be implemented in other regions and countries on condition that available external carbon soil and litter data can be linked to forest inventory plots. The presented procedure includes a specific CS estimation method for each of the following forest pools and subpools (in brackets): living biomass (aboveground and belowground), deadwood (dead trees and snags, coarse woody debris and stumps), litter, and soil. The total CS of the forest was estimated at 86 Tg (185 Mg ha^?1). Soil up to 0.2 m depth, living biomass, litter, and deadwood CSs account, respectively, for 48, 47, 4, and 1 % of the total CS. The analysis of the CS variation within the pools across ecoregions and forest types revealed in particular that: (1) the living biomass CS of broadleaved forests exceeds that of coniferous forests, (2) the soil and litter CSs of coniferous forest exceed those of broadleaved forests, and (3) beech stands come at the top in carbon stocking capacity. Because our estimates differ sometimes significantly from the previous studies, we compared different methods and their impacts on the estimates. We demonstrated that estimates may vary highly, from ?16 to +12 %, depending on the selected methods. Methodological choices are thus essential especially for estimating CO₂ fluxes by the stock change approach. The sources of error and the accuracy of the estimates were discussed extensively.  相似文献