Vascular leakage induced by histamine, bradykinin, serotonin and prostaglandin E₂ in Greyhounds     

PC MILLS  JC NG  AA SEA WRIGHT  DE AUER 《Australian veterinary journal》1993,70(1):21-24

SUMMARY Vascular leakage induced by intradermal injection of histamine, bradykinin and serotonin alone and co-injected with prostaglandin E₂ was measured in Greyhounds using ¹²⁵Iodine-labelled human serum albumin (¹²⁵I-HSA) as a marker in the blood. Histamine and bradykinin produced dose-dependent vascular leakage. At equimolar concentrations, histamine was more than twice as potent as bradykinin. Serotonin did not induce vascular leakage and was irritant. Prostaglandin E₂ did not induce significant vascular leakage (maximum 5μL) when injected alone, but when co-injected with histamine and bradykinin, the vascular leakage of both histamine and bradykinin was increased. This effect was more pronounced if lower concentrations of histamine and bradykinin were injected. The induced vascular leakage was greatest during the first five minutes of lesion development for histamine, during the second five minutes of lesion development for bradykinin, and the synergistic effect of prostaglandin E₂ was maximal during the third five minute period of lesion development.  相似文献   

Comparative transcriptome analysis of female and hermaphrodite flower buds in bitter gourd (Momordica charantia L.) by RNA sequencing     

T. K. Behera  A. R. Rao  R. Amarnath  R. R. Kumar 《The Journal of Horticultural Science and Biotechnology》2016,91(3):250-257

  相似文献   

Comparative analysis of mechanical properties of size film. II. Effect of blend composition and lubricants     

B. K. Behera  Rajesh Mishra  Rashi Gupta 《Fibers and Polymers》2008,9(4):489-494

The advent of very high speed shuttleless looms has increased the importance of sizing. Starch has been the most popular and economic size material. Synthetic binders are also being blended with starch to improve weaving loom efficiency. Blending may lead to give different properties, which can be suited for particular application. In the present study effect of blend composition in modified starch and synthetic size blends have been evaluated and mechanical properties like cohesion power, adhesion power, abrasion resistance, bending rigidity etc were studied. In general, it is being observed that paste characteristics and film properties of polyester resin blends are better but because of economic reasons PVA and acrylic polymers are preferred. In this study the effect of lubricant is also observed. It is analyzed that lubricant improves the various mechanical properties and hence the weaving performance of the material.  相似文献   

Effects of inflammation-associated acute-phase response on hepatic and renal indices in the horse   总被引：1，自引：0，他引：1  

PC MILLS  DE AUER  H. KRAMER  D. BARRY  JC NG 《Australian veterinary journal》1998,76(3):187-194

Objectives To determine the effect of an acute soft tissue inflammatory response on biochemical and haematological indices of hepatic and renal function in the Thoroughbred horse.
Procedure Soft tissue inflammation was induced in four Thoroughbred horses by intramuscular injections of Freunds complete adjuvant. The horses were clinically examined and blood and urine samples were collected before and after the adjuvant injections. Biochemical and haematological indices were measured in samples collected and used to determine the onset of the acute-phase response and to assess hepatic and renal function at this time.
Results After adjuvant injection, significant increases (P< 0.01) in total white (13.1 ± 1.4 times 10⁹/L) and neutrophil (10.2 ± 1.2 times109/L) cell counts, rectal temperature (39.7 ± 0.5A°C) and various plasma protein concentrations, including fibrinogen (6.6 ± 1.2 g/L), haptoglobin (1.3 ± 0.1 g/L) and total protein (88.1 ± 2.7 g/L), indicated the induction of an acute-phase response. This corresponded with significant reductions (P< 0.01) in the plasma elimination half-lives (t½β) sodium bromo-sulphthalein (3.13 ± 0.05 to 2.82 ± 0.07 min) and sodium sulphanilate (38.29 ± 4.04 to 19.60 ± 5.68 min) and reductions in the plasma activities of aspartate aminotransferase, gluta-mate dehydrogenase, creatine kinase, alkaline phosphatase, gamma glutamyl transferase; the urinary creatinine clearance ratios of sodium, chloride and potassium; and the urinary gamma glutamyl transferase-to-creatinine clearance ratios. (All values mean ± SD.)
Conclusions The effects of the acute-phase response on indices of hepatic and renal function in the horse suggest that the disposition of pharmacological agents administered at this time may be altered and that indices of acute inflammation should be interpreted cautiously.  相似文献   

Genetics and molecular mapping of gynoecious (F) locus in cucumber (Cucumis sativus L.)     

Gograj Singh Jat  Anilabh Das Munshi  Tusar Kanti Behera  Harshwardhan Choudhary  Prasanta Dash  Amarnath Ravindran 《The Journal of Horticultural Science and Biotechnology》2019,94(1):24-32

Gynoecious is an important economic trait of cucumber for determinant of earliness and yield, yet genetic mechanism is not well understood for this trait. The experiment was conducted using F₂ mapping population by crossing of PPC-2, a gynoecious and parthenocarpic line with Pusa Uday (monoecious and non-parthenocarpic cultivar). Out of 179 SSR markers screened, 39 markers differentiated the gynoecious and monoecious parents. However, only 17 markers were segregating with F₂ mapping population, those were used for genotyping and linkage map analysis and these markers were placed along with F locus on chromosome 6 covering a total distance of 100.4cM. The SSR markers, SSR13251 and UW020605 were found to be closely linked to gynoecious (F) locus at 1.0 and 4.5 cM, respectively. The segregation of F₂ population of PPC-2 × Pusa Uday and GPC-1 × Punjab Naveen and test crosses for sex type herein suggested that single dominant gene controlled the gynoecious sex expression in cucumber particularly in gynoecious genotypes PPC-2 and GPC-1. Therefore, the monogenic dominant nature of gynoecious sex identified in the present experiment and SSR markers closely linked to the F locus will be useful in marker-assisted backcross breeding for transfering gynoecious trait into horticulturally desirable varieties.  相似文献   

Long‐term changes in the floristic composition and soil characteristics of reclaimed sodic land during eco‐restoration     

Pankaj Kumar Srivastava  Baleshwar  Soumit Kumar Behera  Nandita Singh  Radhey Shyam Tripathi 《植物养料与土壤学杂志》2011,174(1):93-102

The study was conducted under the “Uttar Pradesh Sodic Lands Reclamation Project” to examine changes that occurred in the reclaimed sodic land in two districts of Uttar Pradesh, India. The study focuses on long‐term seasonal changes in the floral diversity and soil characteristics of the reclaimed sodic land over a period of 10 y. The changes in the floristic composition, plant density, and soil characteristics (microbial biomass carbon [MBC], pH, exchangeable‐sodium percentage (ESP), and electrical conductivity) were compared among the different study plots after different years of sodic‐land reclamation. The study plots comprised reclaimed land with rice–wheat cultivation; semireclaimed land under rice cultivation only and nonreclaimed barren sodic land. There was a significant variation in the floristic composition of the three study plots. Dominance in the floristic composition was shifted from monocotyledonous weeds in the nonreclaimed sodic land to dicotyledonous weeds in the reclaimed land after 10 y of reclamation. Among the soil characteristics, the most remarkable changes were observed in soil MBC and ESP during the course of sodic‐land reclamation. Soil MBC increased up to 480% and ESP values decreased up to 79% in the reclaimed plots with reference to the nonreclaimed plots. The soil amelioration was more pronounced in the upper layer (0–30 cm) as compared to the lower layer (below 30 cm depth). A positive significant correlation was revealed between soil MBC and floristic composition of the reclaimed plots. These changes in floristic composition and soil characteristics could be used as good indicators of the eco‐restoration of the sodic lands. The present study provides useful insights in understanding the temporal progress of eco‐restoration in the reclaimed sodic lands.  相似文献   

Relationship between vitamin B₁₂ and cobalt concentrations in bovine liver     

A. MITSIOULIS  PC BANSEMER  T-S KOH 《Australian veterinary journal》1995,72(2):70-70

  相似文献   

The Origin of Membrane Vesicles in Ram Seminal Plasma     

R El-Hajj Ghaoui  PC Thomson  G Evans  WMC Maxwell 《Reproduction in domestic animals》2006,41(2):98-105

The hypothesis tested in this study was that the membrane vesicles present in ram seminal plasma are of testicular origin, rather than being secreted by the accessory sex glands as has been previously reported for a number of species. Membrane vesicles were present in cellular extracts from reproductive organs and accessory sex glands of six rams, and in the seminal plasma of a further eight rams. When four of the latter rams were subjected to vasectomy, to isolate ejaculate contents to only the secretions of the accessory sex glands, the vesicles were largely eliminated from their ejaculates, while vesicles were still present in the ejaculates of the four control rams. The constituents of the cytoplasmic droplets and membrane vesicles derived from the seminal plasma were compared by transmission electron microscopy (TEM). Vesicles present in the cytoplasmic droplets were similar in morphology but smaller on average than those in the seminal plasma. It was concluded that the membrane vesicles in ram seminal plasma originate from either the cytoplasmic droplets, or a combination of vesicles from the droplets and the epididymis.  相似文献   

Autologous Whole Ram Seminal Plasma and its Vesicle-free Fraction Improve Motility Characteristics and Membrane Status but not In Vivo Fertility of Frozen–Thawed Ram Spermatozoa     

R El-Hajj Ghaoui  PC Thomson  T Leahy  G Evans  WMC Maxwell 《Reproduction in domestic animals》2007,42(5):541-549

Motility characteristics (assessed subjectively and with computer-assisted semen analysis) and membrane status (after staining with chlortetracycline) of washed and non-washed frozen-thawed ram spermatozoa were evaluated after incubation in buffer and buffer containing autologous whole seminal plasma or one of its two fractions: the pellet of membrane vesicles obtained by ultracentrifugation (and used at three times normal protein concentration) or the vesicle-free supernatant fraction. Whole seminal plasma and supernatant, but not membrane vesicles, improved the motility characteristics of spermatozoa after 3 and 6 h of post-thaw incubation compared with the control buffer. Resuspension and incubation with whole seminal plasma, supernatant or membrane vesicles lowered the proportion of acrosome-reacted frozen-thawed spermatozoa compared with the control buffer. Unwashed frozen-thawed semen from three rams, incubated with autologous whole seminal plasma or its fractions and inseminated using cervical or intrauterine artificial insemination, had no effect on pregnancy rates of ewes in synchronized oestrus. However, fertility was higher after laparoscopic than cervical insemination (44.9 vs 12.3%, p < 0.001). In conclusion, resuspension and incubation of frozen-thawed ram spermatozoa in autologous whole seminal plasma or its vesicle-free supernatant fraction improved their motility characteristics and, with membrane vesicles, membrane status, but these benefits were not reflected in improved fertility after cervical or intrauterine insemination.  相似文献   

Haemato-biochemical alterations and oxidative stress associated with naturally occurring porcine circovirus2 infection in pigs     

Rajesh  J. B.  Rajkhowa  S.  Dimri  U.  Prasad  H.  Mohan  N. H.  Hmar  L.  Sarma  K.  Chethan  G. E.  Behera  P.  Jaganmohanarao  G.  Behera  S.  Zosangpuii 《Tropical animal health and production》2020,52(5):2243-2250

Tropical Animal Health and Production - Porcine circovirus2 (PCV2) infection in pigs is one of the major causes of economic loss to the farmers in terms of low production, slow growth and increase...  相似文献