The feral horse foot. Part A: observational study of the effect of environment on the morphometrics of the feet of 100 Australian feral horses     

BA Hampson  MA de Laat  PC Mills  CC Pollitt 《Australian veterinary journal》2013,91(1-2):14-22

  相似文献   

The feral horse foot. Part B: radiographic,gross visual and histopathological parameters of foot health in 100 Australian feral horses     

BA Hampson  MA de Laat  PC Mills  DM Walsh  CC Pollitt 《Australian veterinary journal》2013,91(1-2):23-30

  相似文献   

Development of real-time PCR assay for the detection of Mycoplasma bovis     

Behera  S.  Rana  R.  Gupta  P. K.  Kumar  D.  Sonal  Rekha  V.  Arun  T. R.  Jena  D. 《Tropical animal health and production》2018,50(4):875-882

Mycoplasma bovis is one of the important bovine mycoplasma involved in economically important clinical conditions like respiratory diseases, otitis media, and mastitis. The present study was undertaken with the objective of developing a SYBR Green dye-based real-time PCR assay targeting uvrC gene for the diagnosis of M. bovis. The analytical sensitivity and specificity of the assay were evaluated. The test showed 10³-fold more sensitivity than conventional PCR and detected down to 100 fg level of DNA. It was found to be specific, as no cross reactivity was shown with other related bacteria and Mycoplasma species. The developed assay was able to detect down to 40 copies of uvrC gene from spiked bovine milk samples. At present, this developed assay may be used as a valuable diagnostic tool for the detection of Mycoplasma bovis.

  相似文献   

Relationship between endometritis and oxidative stress in the follicular fluid and luteal function in the buffalo          下载免费PDF全文

BK Behera  CG Sharma  SK Singh  H Kumar  RK Chaudhari  AS Mahla  GK Das  N Krishnaswamy 《Reproduction in domestic animals》2016,51(5):844-847

In this study, alteration in the follicular fluid composition and luteal function was investigated in the buffalo with endometritis. Genitalia were classified into cytological and purulent endometritis on the basis of polymorphonuclear cell cut off while non‐endometritis served as control (n = 10/group). In the follicular phase, the number of surface follicles was counted, diameter of the largest follicle was measured and the follicular fluid was assayed for total protein, cholesterol, malondialdehyde (MDA), total antioxidant capacity (TAC), oestradiol (E₂) and progesterone (P₄). The P₄ content of corpus luteum during mid‐luteal phase was estimated by radioimmunoassay. Ovaries from the follicular phase of oestrous cycle showed no significant difference in the total number of surface follicles, size of the largest follicle and volume of follicular fluid in the buffaloes with and without endometritis (p > .05). However, the antral fluid of the largest follicle from the genitalia of buffalo with cytological and purulent endometritis showed a significant decrease in the concentration of total protein, cholesterol, TAC and E₂ and a significant increase in the concentration of MDA and P₄ (p < .05). The results indicated that there is an association between endometritis and decreased ovarian function.  相似文献   

Characterisation of cultivated breeding lines of eggplant (Solanum melongena L.) and related wild Solanum species from India     

Chingakham Premabati Devi  Anilabha D. Munshi  Tusar Kanti Behera  Harshwardhan Choudhary  Partha Saha 《The Journal of Horticultural Science and Biotechnology》2016,91(1):87-92

Immature fruit of cultivated species of eggplant (Solanum melongena L.) are commonly used as a summer vegetable in India. Rich morphological variation exists among the cultivated species of eggplant in different growing regions of the country. We have characterised 24 breeding lines of Solanum spp., including 20 eggplant cultivars and four wild species of eggplant, based on 13 morphological characters using Mahalonabis D² statistics. All 24 breeding lines of Solanum spp., including the 20 eggplant cultivars and four wild species, were grouped into four clusters by agglomerative clustering. Cluster II and Cluster IV contained the most accessions (eight each), while Cluster I and Cluster III had four accessions each. The highest inter-cluster (D²) distance (158.33) was observed between Cluster I and Cluster II, followed by Cluster I and Cluster III (108.48), and Cluster II and Cluster IV (102.96), which indicated that accessions in Cluster I and Cluster II were more divergent than those in the other clusters. The highest intra-cluster distance (5.80) was observed in Cluster IV, with eight genotypes, and the lowest intra-cluster distance (2.21) was observed in Cluster II, also with eight genotypes. The intra-cluster distances in all four clusters were lower than the inter-cluster distances, which indicated that genotypes within the same cluster were closely related. Genotypes in Cluster IV had the maximum number of flowers per cluster (3.63), the highest number of fruit per cluster (3.25), and number of fruit per plant (208.63), which revealed that genotypes could be selected from Cluster IV for these characters. The first three principal components (PC1, PC2, and PC3) accounted 73.99% of the total variation among the 24 genotypes. These phenotypic data increase the feasibility of prioritising breeding lines in a crossing programme based on the uniqueness of their desirable morphological traits.  相似文献   

Outbreak of avian influenza virus H5N1 in India     

Tosh C  Murugkar HV  Nagarajan S  Bhatia S  Pateriya AK  Behera P  Jain R  Kumar S  Khandia R  Vanamayya PR  Dubey SC  Ahlawat SP 《The Veterinary record》2007,161(8):279

  相似文献   

Molecular characterization of RNA and protein synthesis during a one-step growth curve of bovine viral diarrhoea virus in ovine (SFT-R) cells     

N. Mishra  B.S. Mathapati  K. Rajukumar  R.K. Nema  S.P. Behera  S.C. Dubey 《Research in veterinary science》2010,89(1):130-132

The aim of this study was to determine the kinetics of noncytopathic bovine viral diarrhoea virus (BVDV) multiplication and synthesis of BVDV specific RNA and proteins in ovine cells (SFT-R) during a one-step growth curve. The virus titre and RNA level were determined by focus-forming assay and real time RT-PCR. The RNA synthesis was detected by Northern blot while synthesis of E2 and NS3 proteins was assayed by immunohistochemistry and Western blot. The results showed that synthesis of viral RNA is initiated at 4 h, NS3 and E2 proteins are detectable at 6-7 h and the replication cycle is complete at 10-12 h. Additionally, we provide evidence that NS2-3 protein was cleaved in ovine cells early during infection and in proliferated leukocytes of acutely infected sheep. This study showed that synthesis of BVDV RNA and proteins in ovine cells occurs at similar times as found in bovine cells.  相似文献   

Retrospective study on the occurrence of canine lymphoma and associated breed risks in a population of dogs in NSW (2001–2009)          下载免费PDF全文

PPY Yau  NK Dhand  PC Thomson  RM Taylor 《Australian veterinary journal》2017,95(5):149-155

  相似文献   

Nano zinc vis‐à‐vis inorganic Zinc as feed additives: Effects on growth,activity of hepatic enzymes and non‐specific immunity in rohu,Labeo rohita (Hamilton) fingerlings     

Aziz Hasan Mondal  Truptimayee Behera  Priyabrat Swain  Rakesh Das  Satya Narayan Sahoo  Sudhansu Sekhar Mishra  Jayakrushna Das  Koushik Ghosh 《Aquaculture Nutrition》2020,26(4):1211-1222

The effects of dietary administration of inorganic zinc (zinc sulphate, ZnSO₄) and nano zinc (zinc oxide nanoparticles, ZnO‐NP) were evaluated in rohu, Labeo rohita fingerlings. Fish were fed with a basal diet (Control) supplemented with ZnSO₄ (T1, T2 and T3) and ZnO‐NP (T4, T5 and T6) at 10, 20 and 30 mg/kg, respectively, for a duration of 45 days. The results revealed that fish fed diet containing 20 mg ZnO‐NP per kg (T5) had the highest weight gain and specific growth rate (SGR, % per day), which was significantly different (p < .05) from the other experimental diets. Significantly (p < .05), higher activities of the digestive and metabolic enzymes were recorded in the fish fed ZnO‐NP containing diets as compared to the diets containing inorganic Zn or control diet. The maximum serum glucose and protein levels were noted in fish reared on diet T5. Both SGOT and SGPT activities were significantly increased in fish fed Zn‐supplemented diets (T1 to T6), as compared to the control group. Similarly, innate immune parameters were improved with feeding Zn incorporated diets. The highest phagocytic (40.74 ± 0.65%) and respiratory burst (0.33 ± 0.001, OD 630nm) activities were recorded in the fish fed diet containing ZnO‐NPs at 20 mg/kg (T5). The maximum superoxide production and serum peroxidase activity were detected in the fish fed T5 and T6 diets. Overall, results indicated that short‐duration feeding (≤45 days) of dietary ZnO‐NP (20 mg/kg) improved growth, enzyme activity, serum biochemical parameters and immune function in rohu fingerlings.  相似文献   

Conventional and contemporary approaches for drought tolerance rice breeding: Progress and prospects     

C Anilkumar  Rameswar Prasad Sah  Radha Beena  Muhammed Azharudheen TP  Awadesh Kumar  Sasmita Behera  NC Sunitha  SK Pradhan  KR Reshmi Raj  Parameswaran C  BC Marndi  Anil Kumar Singh 《Plant Breeding》2023,142(4):418-438

Drought is becoming a major threat to rice farming across the globe owing to the depletion of water tables in rice-growing belts. Drought affects rice plants at multiple stages, causing damage at morphological and physio-biochemical levels, leading to severe losses that exceed losses from all other stresses. The amalgamation of conventional breeding methods with modern molecular biology tools and biometrical methods could help accelerate the genetic gain for drought tolerance in rice. Many drought-tolerance traits with genetic determinants have been identified and exploited for tolerance rice variety breeding. The integration of genome-wide association study and genomic selection tools with speed breeding shortened the breeding cycle and aided in rapid improvement of genetic gain. In this review, we emphasized the progress made through classical breeding as well as the limitations and usefulness of current genomic methods in improving drought tolerance. We briefly addressed methods for identifying genetic determinants for drought tolerance and deploying them through genomics-assisted breeding programmes to develop high-yielding drought-tolerant rice cultivars.  相似文献